ARSENIC None Random or 24hr. Room Temp. Avoid all seafood for 72 hrs. prior to collection.

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1 1 Boric Acid tablet = 1 gram. PLEASE NOTE: URINE TESTING (RANDOM/24HR) SPECIMEN REQUIREMENTS 1. ANY INSTRUCTIONS TO FREEZE ARE IN REFERENCE TO ALIQUOTS MADE AFTER COLLECTION IS COMPLETE. 2. REFRIGERATE DURING COLLECTION UNLESS ROOM TEMP. 30 ml vol. of several preservatives is for 24 hr. collection only. Random urines- add preservative necessary to achieve ph. TEST Preservative Collect Time Storage Other Instructions ALDACTONE None Random or 24hr. Refrigerate- Protect from light ALDOSTERONE 1 g Boric Acid/L 24 hour Refrigerate HCL not acceptable AMINO ACIDS, QNT None Random or 24hr. Freeze AMINOLEVULINIC 30 ml 30% Glacial Acetic 24 hr. Freeze, Protect from light Acidify to ph < 6.0 ACID (ALA).5ml 30% Glacial Acetic Acid Random Freeze, Protect from light to 10 ml random urine. AMYLASE None 24 hr. Room Temp. AMMONIA None Random or 24 hr. Freeze in 2 hrs. (4hrs. Max) ARSENIC None Random or 24hr. Room Temp. Avoid all seafood for 72 hrs. prior to collection. BETA-2 MICRO- None Random only Refrigerate Final ph 6-8 (with 1N NaOH or 6N HCL) GLOBULIN See book for patient instructions. BENZENE METABOLITE None Random Room Temp. Industrial exposure monitoring. end of employee's shift. BICARBONATE None Random Refrigerate BK VIRUS None Random Freeze immediately

2 CADMIUM None Random or 24hr. Room Temp. CALCIUM 10 ml 6N HCL- *In-House* 24 hr. Refrigerate See specimen instructions- Chemistry. Must add acid 10 ml 6N HCL- *Referred* 24 hr. Refrigerate prior to start of collection. acidify by drop to desired ph Random Refrigerate ph < 4. CANNABINOIDS None Random Refrigerate CARBOHYDRATES None Random Freeze Immediately CATECHOLAMINES, 30 ml 6N HCL 24 hr. Refrigerate Final ph 1-3 with 6N HCL. No Boric or Acetic Acid. FRACTIONATED Acidify by drop to desired ph CHLORIDE None- *In-House* Random or 24 hr. Refrigerate None- *Referred* Random or 24 hr. Refrigerate CHROMIUM None Random Room Temp. CITRIC ACID 30 ml 6N HCL 24 hr. Refrigerate Final ph 1-3 w/6n HCL. No Boric or Acetic Acid. Freeze if no acid added. COBALT None Random or 24 hr Room Temp. COLLAGEN CROSS- LINKED N. TELOPEPTIDE See N-Telopeptide COPPER None Random or 24 hr. Room Temp. CORTISOL, FREE BY 1g Boric Acid/Liter 24 hr. Refrigerate Acid must be added at start or w/i 4hrs. Of completion ICMA OR HPLC 30 ml 6N HCL also accept. of specimen. CREATINE None 24 hr. Freeze Immediately Acid converts Creatine to Creatinine. CREATININE None. Boric Acid or 6N HCL Random or 24 hr Refrigerate Creatinine Clearance requires blood collection as also ok- *In-house* well as height and weight. None or 6N HCL- *Referred* Random or 24 hr. Refrigerate CYCLIC AMP None Random Freeze Immediately

3 CYSTINE 30 ml 6N HCL 24 hr. Freeze Immediately Final ph 1-3 with 6N HCL, No Boric Acid None Random Freeze Immediately DOPAMINE EPINEPHRINE See Catecholamines-Fract. See Catecholamines-Fract. GLUCOSE 1 g Boric Acid or NaF 24 hr Refrigerate HEAVY METAL EVAL None Random or 24 hr. Room Temp. Includes Arsenic, Lead and Mercury 5 HIAA (HYDROXY- None. 24 hr. Refrigerate See book for Patient Instructions. INDOLEACETIC ACID) Boric Acid or 6N HCL ok. HISTAMINE None 24 hr. Freeze Immediately HOMOCYSTEINE None Random or 24 hr. Freeze Immediately HOMOGENTISIC ACID None Random Freeze Immediately HOMOVANILLIC ACID 30 ml 6HCL 24 hr. Refrigerate Final ph 1-3 W 6N HCL. Refrigerateacidify drop by drop to Random See book for pt. instructions. desired ph. 17-HYDROXYCORTICO- STEROIDS 1g Boric Acid or 30 ml 6N HCL 24 hr. MUST ADD PRESERVATIVE AT START OF COLLECTION. FREEZE No exception. With preservative added at start can refrigerate up to 72 hrs. before freezing. See Patient instructions. Consult Physician. HYDROXYPROLINE 30 ml 6N HCL 24 hr. Refrigerate Final ph <3 with 6N HCL. FREE &/OR TOTAL See book for pt. instructions. IMMUNOFIXATION None Random or 24 hr Refrigerate IODINE None Random or 24 hr. Refrigerate IRON None 24 hr. only Refrigerate Metal/Glue Free Container. 17 KETOGENIC 1 g Boric Acid/L or 30 ml 24 hr. Refrigerate See book for Pt. Instructions. (Consult physician) STEROIDS 6N HCL.

4 17 KETOSTEROIDS 1 g Boric Acid/L or 30 ml 24 hr. Refrigerate 6N HCL LEAD None Random or 24 hr. Room Temp. LIGHT CHAINS None Random or 24 hr. Refrigerate (kappa &/or Lambda) LIPASE None Refrigerate LYSOZYME None Random Freeze Immediately MAGNESIUM 10 ml 6N HCL 24 hr Refrigerate Final ph with 6N HCL. MANGANESE None Random Room Temp. MERCURY None Random or 24 hr Room Temp. METANEPHRINES 30 ml 6N HCL 24 hr. Refrigerate Final ph < 3 with 6N HCL. No Boric or Acetic Acid. See patient preparation instructions- Consult Physician. METHYLPHENIDATE None Random Freeze Immediately MICROALBUMIN None Random or 24 hr Refrigerate MUCOPOLYSACCHARI DES None Freeze Immediately MYOGLOBIN None Random Refrigerate NICKEL None Random or 24 hr Room Temp. NOREPINEPHRINE See Catecholamines, Fractionated N-METHYLHISTAMINE None Refrigerate N-TELOPEPTIDE None-2nd am void or 24 hr. Freeze Can refrig. up to 72 hrs. Do not submit first a.m. void. (NTX) OLIGOSACCHARIDES None Freeze immediately

5 ORGANIC ACIDS None Random Freeze immediately OSMOLALITY None- *In-house* Random or 24hr Refrigerate None- *Referred* Random or 24hr Refrigerate OSTEOMARK See N-Telopeptide OXALATE 30 ml 6N HCL 24 hr. Room Temp. or Refr. ph <2.0. Boric acid not acceptable. Avoid Vit. C 24hr. Prior PHENOL None Random, end of Room Temp. day/work/shift. PHENYLALANINE None Random or 24 hr. Freeze immediately PHOSPHOROUS 30 ml 6N HCL Random or 24 hr. Refrigerate PORPHOBILINOGEN 30 ml 30% Glacial Acetic 24 hr. Freeze- protect from light. Can use Na Carbonate, but not if ALA is also ordered. (PBG).5 ml 30% Glacial Acetic Random to 10 ml Random Urine PORPHYRINS None Random or 24 hr. Refrigerate- protect from light. Na Carbonate, 5 mg is also acceptable for preservative. POTASSIUM None- *In-house* 24 hr. Refrigerate. None- *Referred* 24 hr. Refrigerate. PROSTAGLANDIN D2 None 24 hr. Freeze immediately No aspirin, Indomethicin or anti-inflammatory meds if possible for 48 hrs. prior. Consult physician. PROSTAGLANDIN E1 None Freeze No aspirin/anti-inflammatory meds. PROSTAGLANDIN E2 None Freeze No aspirin/anti-inflammatory meds. PROSTAGLANDIN F2 None 24 hr. Freeze PROTEIN None- *In-house* Random or 24 hr. Refrigerate None- *Referred* Random or 24 hr. Refrigerate PROTEIN ELECTROPHORESIS None Random or 24hr Room Temp.

6 RITALIN See Methylphenidate SELENIUM None Random or 24hr Room Temp. SEROTONIN See 5-HIAA 5-Hydroxytryptamine is no longer available. SODIUM None- *In-house* Random or 24 hr. Refrigerate None- *Referred* Random or 24 hr. Refrigerate THALLIUM None Random or 24hr Room Temp. UREA NITROGEN None Random or 24 hr. Refrigerate URIC ACID Add 10 ml 5% NaOH In-house Random or 24 hr. Refrigerate ph should be >6.5. See instructions in Chem. Proc. prior to start of collection. Random or 24 hr. Refrigerate Reference- None Random or 24 hr. Refrigerate UROBILINOGEN See UA dipstick or see porphyrins. VANILLYMANDELIC 30 ML 6N HCL 24 hr. Refrigerate Final ph 1-3 with 6N HCL. (VMA) Acidify drop by drop to Random (may be kept refrigerated without preservative up to desired ph. 5 days then acidified, per LCA) XYLENES None Room Temp. XYLOSE TOLERANCE None 5hr. Collection Room Temp. See instructions- Depending on Dr. order- blood collection may also be requested. ZINC None Random or 24hr. Room Temp.

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