Urine Preservatives Collection and Transportation for 24-Hour Urine Specimens

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1 Preservatives Collection and Transportation for 24-Hour Specimens The Department of Laboratory Medicine and Pathology at Mayo Clinic initiated a study to determine stability of analytes under different preservative conditions. This reference chart, built from that study, will enable you to select either temperature conditions or preservatives to maximize the number of tests that can be performed on a single 24-hour collection. Note: See the Test Catalog for test-specific collection requirements. *Protect from light. Refrigerate Frozen Boric Aldosterone, 24 ALDU OK OK OK OK Preferred OK No No No OK No Aldosterone with Sodium, 24 Alpha-1-Microglobulin, 24 ALDNA OK OK OK OK Preferred OK No No No OK No A1M24 OK Preferred OK OK OK OK No No No OK OK Aluminum, 24 ALU OK Preferred OK No OK OK No OK OK No OK Ammonium, 24 AMMO OK Preferred OK No No Preferred No No No No No Arsenic, 24 ASU OK Preferred OK No OK OK No OK No No OK Arsenic Fractionation, 24 Arylsulfatase A, 24 Hour, ASFR OK Preferred OK No OK OK No OK No No OK ARSU No Required No No No No No No No No No Cadmium, 24 CDU OK Preferred OK No OK OK No OK OK No OK Calcium, 24 CALU OK Preferred OK OK OK OK No OK OK OK OK Catecholamine Fractionation, Free, 24 CATU No OK OK OK Preferred No No OK OK OK No Chloride, 24 CLU OK Preferred OK OK OK OK No No No OK Preferred Chromium, 24 CRU OK Preferred OK No No No No No No No OK 14 days 28 days 72 hours Page 1 of 5 04/ days Frozen NO 28 days 72 hours 28 days

2 Preservatives Collection and Transportation for 24-Hour Specimens Citrate Excretion, 24 Hour, Refrigerate Frozen Boric CITR No OK OK OK No Preferred No No No OK No Cobalt, 24 COU OK Preferred OK No OK OK No OK OK No OK Copper, 24 CUU OK Preferred OK No OK OK No OK OK No No Cortisol/Cortisone, Free, 24 COCOU No OK OK Preferred OK OK OK No No OK No Cortisol, Free, 24 CORTU No OK OK Preferred OK OK OK No No OK No Creatinine, 24 CTU OK Preferred OK OK OK OK OK OK OK OK Preferred Creatinine Clearance, Serum and 24-Hour Cystinuria Profile, Quantitative, 24 CRC Preferred OK OK OK OK OK OK OK OK OK Preferred CYSQN No OK OK No No Preferred No No No No No 28 days 28 days Refrig Preferred 30 days 30 days 30 days Refrig Preferred Frozen Preferred 14 days Refrig with Toluene OK 2,3-Dinor-11Beta- Prostaglandin F2 Alpha, 23BPG No Preferred OK OK OK OK OK No No No OK 8 hours 30 days Electrophoresis, Protein, 24 Electrophoresis, Protein, 24 INTERNAL ONLY EPU OK Preferred OK No No OK No No No OK Preferred PEU OK Preferred OK No No OK No No No No Preferred Gadolinium, 24 GDU OK Preferred OK No No OK No OK OK No OK Glucose, 24 GLUR No OK OK Preferred OK OK No OK OK OK No Heavy Metals Screen, 24 Homovanillic (HVA), 24 5-Hydroxyindoleacetic (5- HIAA), 24 HMSU No Preferred OK No OK OK No OK No No No HVA No No No No Preferred No No OK No No No HIAA No OK OK OK Preferred OK OK OK OK OK No 72 hours 72 hours 28 days Refrig Preferred 56 days 365 days Page 2 of 5 04/2016

3 Preservatives Collection and Transportation for 24-Hour Specimens Immunofixation, 24 Hour, Immunoglobulin Total Light Chains, Inherited Conjugated Hyperbilirubinemias, 24 Hour, Refrigerate Frozen Boric IFXU OK Preferred OK No No OK No No No OK Preferred TLCU OK <72 Hours Preferred OK No No No No No No No <72 Hours OK CI No No No No No No Required* No No No No Iodine, 24 UIOD OK Preferred OK No OK No No No No No OK Lead, 24 PBU OK Preferred OK No OK OK No OK OK No OK Magnesium, 24 MAGU OK Preferred OK OK OK OK No OK OK OK OK Manganese, 24 MNU OK Preferred OK No No No No No No No OK Mercury, 24 HGU No Preferred OK No OK OK No OK OK No No Metanephrines, Fractionated, 24 N-Methylhistamine, 24 Hour, Microalbuminuria, 24 Hour, METAF No OK OK Preferred Preferred OK OK OK OK OK OK NMHIN OK Preferred OK OK OK OK OK OK OK OK OK MA24 OK Preferred OK OK No OK No No No OK OK Molybdenum, 24 MOLU OK Preferred OK No OK OK No OK No No OK Monoclonal Protein Study, 24 MPSU OK Preferred OK No No OK No No No OK Preferred Nickel, 24 NIU OK Preferred OK No OK No No No OK No OK Nitrogen, Total, 24 NITU OK OK Preferred No OK OK OK OK No OK OK NTX-Telopeptide, 24 Hour, NTXPR No OK <5 days Preferred OK No OK No No No No No Oxalate, 24 OXU No OK OK No No Preferred No No No OK No 72 hours 20 days 72 hours Frozen Preferred Refrig NO Refrig Preferred 146 days 146 days 146 days 28 days 72 hours Refrig Preferred 8 days 24 hours 28 days 72 hours 28 days Frozen Preferred 30 days 5 days Page 3 of 5 04/2016 Protect from light

4 Preservatives Collection and Transportation for 24-Hour Specimens Refrigerate Frozen Boric Phosphorus, 24 POU OK Preferred OK OK OK OK No OK OK OK Preferred ph, 24 UPH24 No OK OK No No Preferred No No No OK No Porphyrins, Quantitative, 24 PQNU No No No No No No Required* No No No No Potassium, 24 KUR OK Preferred OK OK OK OK OK OK OK OK Preferred Protein, Total, 24 PTU OK Preferred OK OK No OK No No No OK Preferred Retinol-Binding Protein, 24 RBP24 Preferred OK OK OK No OK No No No OK Preferred Selenium, 24 SEU OK Preferred OK No OK OK No OK OK No OK Serotonin, 24 SERU No Preferred OK No Preferred OK No No OK OK OK Sodium, 24 NAU OK Preferred OK OK OK OK No OK OK OK Preferred Sulfate, 24 SULFU No Preferred OK OK No OK OK No No OK Preferred Supersaturation Profile, 24 SAT24 No OK OK No No Preferred No No No No No Thallium, 24 TLU OK Preferred OK No OK OK No OK OK No OK Urea, 24 URAU No OK OK Preferred OK OK OK OK OK OK No Uric, 24 URCU No OK OK Preferred No OK OK No No OK No Vanadium, 24 VU OK Preferred OK No No No No No No No No Vanillylmandelic (VMA), 24 Xanthine and Hypoxanthine, 24 VMA No No No OK Preferred No No OK OK No No XAN No OK Preferred No No No No No No No No Frozen Refrig NO 28 days Frozen Preferred 14 days 28 days Frozen Preferred Page 4 of 5 04/2016 Protect from light

5 Preservatives Collection and Transportation for 24-Hour Specimens Refrigerate Frozen Boric Zinc, 24 ZNU OK Preferred OK No OK OK No OK OK No OK 28 days The conditions and concentrations of urine preservatives are defined for 24-hour collections as follows: Room temperature (21-25 degrees C) Refrigerated 4 degrees C Frozen -20 degrees C 6N HCl 30 ml per 24-hour collection 25 ml per 24-hour collection Na 2 CO 3 (crystals) 5 g per 24-hour collection Toluene 30 ml per 24-hour collection 6N HNO 3 15 ml per 24-hour collection Boric (crystals) 10 g per 24-hour collection Thymol (10% in isopropanol) 10 ml per 24-hour collection Preservative and condition guidelines have been evaluated specifically for Mayo Medical Laboratories assays. If you have questions regarding the use of preservatives, contact Mayo Medical Laboratories at or Page 5 of 5 04/2016