CAMÉRAS EMCCD POUR LA QUANTIFICATION DE FLUORESCENCE IN VIVO
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2 CAMÉRAS EMCCD POUR LA QUANTIFICATION DE FLUORESCENCE IN VIVO Yoann Gosselin, B.Ing., M.Sc.A. Ingénieur en application 2
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10 TECHNOLOGIE BREVETÉE POUR LA CAMÉRA LA PLUS SENSIBLE 6
11 TECHNOLOGIE BREVETÉE POUR LA CAMÉRA LA PLUS SENSIBLE 7
12 TECHNOLOGIE BREVETÉE POUR LA CAMÉRA LA PLUS SENSIBLE GRACIEUSETÉ DE LABEO TECH 8
13 QU EST-CE QU UNE CAMÉRA EMCCD? 9
14 PRINCIPALES CARACTÉRISTIQUES D UN DÉTECTEUR EMCCD 10
15 PRINCIPALES CARACTÉRISTIQUES D UN DÉTECTEUR EMCCD EMCCDs are mostly sensitive in the visible spectral range Quantum Efficiency over 90% from 500 nm to 650 nm 11
16 PRINCIPALES CARACTÉRISTIQUES D UN DÉTECTEUR EMCCD Detector architecture similar to frame-transfer CCDs Nüvü Caméras 12
17 PRINCIPALES SOURCES DE BRUIT DANS UNE EMCCD Bruit thermique Bruit d injection de charges (CIC) Excess Noise Factor 13
18 REFROIDISSEMENT DU DÉTECTEUR -85 C Nüvü Caméras 14
19 REFROIDISSEMENT DU DÉTECTEUR Diminuer la température du détecteur vient aussi affecter la capacité du détecteur à déplacer les charges de pixel en pixel. Nüvü Caméras EMCCD technology Typical EMCCDs -85 C cooling, 10 MHz readout speed EM gain of 5000 for Nüvü Caméras EM gain of 1000 for other EMCCDs 15
20 LINEAR MODE VS PHOTON COUNTING Qu est-ce que l excess noise factor? Quel est son impact sur la qualité de l image? Même effet sur le RSB que si l efficacité quantique diminuait de moitié COMMENT AUGMENTER ENCORE DAVANTAGE LE RSB? SOLUTION : UTILISER LE MODE COMPTAGE DE PHOTON (PC) 16
21 LINEAR MODE VS PHOTON COUNTING COMMENT ÉLIMINER L ENF? Threshold Lost events 17
22 PRINCIPALES SOURCES DE BRUIT DANS UNE EMCCD Bruit thermique -> Refroidissement avec cryostat unique Bruit d injection de charges (CIC) -> Électronique brevetée Excess Noise Factor -> Comptage de photons efficace 18
23 CAMÉRAS ET APPLICATIONS 19
24 CAMÉRAS LN2 & TEC 20
25 RECHERCHE BIOMÉDICALE AVEC NÜVÜ 21 NUVU CAMERAS INC 2015
26 BIOMEDICAL RESEARCH WITH NÜVÜ SMALL ANIMAL IMAGING The CCy5.5 fluorophore was injected intravenously to a concentration of 0.1 mg/ml. The colored zone shows the scanned area obtained by superposing fluorescence images to controls. NUVU CAMERAS INC
27 BIOMEDICAL RESEARCH WITH NÜVÜ RAMAN IMAGING Raman imaging using a tunable Bragg filter with Nüvü s EMCCD technology is 60 times faster than conventional point-by-point mapping with standard CCD cameras. In-vivo and ex-vivo applications are now at reach, paving the way for real time tumor detection during surgery NUVU CAMERAS INC
28 BIOMEDICAL RESEARCH WITH NÜVÜ PHARMACEUTICAL HYPERSPECTRAL IMAGING Detection of four membrane proteins with Quantum Dots and a synaptic marker on fixed hippocampal neurons (each image 75 X 45 µm) The possibility of acquiring hyperspectral data at the rate of 67 fps makes the Multiplex Microscopy Hyperspectral Imaging Platform a great tool to study rapid exchanges as those that occur in the central nervous system. Nüvü Caméras enhances rapid neuronal imaging platform for drug effectiveness assessment NUVU CAMERAS INC
29 BIOMEDICAL RESEARCH WITH NÜVÜ FLUORESCENCE-GUIDED SURGERY NUVU CAMERAS INC
30 BIOMEDICAL RESEARCH WITH NÜVÜ FLUORESCENCE-GUIDED SURGERY NUVU CAMERAS INC
31 BIOMEDICAL RESEARCH WITH NÜVÜ SINGLE MOLECULE IMAGING Using an EMCCD camera in photon-counting mode enables visualization of single molecules and photobleaching behaviour at a much lower laser power compared with analog mode and thus may provide a better time resolution NUVU CAMERAS INC
32 RECHERCHE BIOMÉDICALE AVEC NÜVÜ RECHERCHE À VENIR MICROSCOPY Super resolution FRET / BRET Simultaneous flow cytometry / single cell imaging Bayesian analysis of blinking and bleaching (3B) Cerenkov luminescence imaging GENETICS / PHARMA High-throughput readers Genome sequencing Microfluidic systems 28 NUVU CAMERAS INC 2015
33 ASTRONOMIE & PHYSIQUE AVEC NÜVÜ 29 NUVU CAMERAS INC 2015
34 ASTRONOMIE & PHYSIQUE AVEC NÜVÜ ÉCOSYSTÈME D INNOVATION PUISSANT NUVU CAMERAS INC
35 ASTRONOMIE & PHYSIQUE AVEC NÜVÜ ÉCOSYSTÈME D INNOVATION PUISSANT FUTURE SPACE APPLICATIONS Direct exoplanet observation Asteroids tracking Space debris monitoring UV spectroscopy Astero-seismology Ocean and coastal monitoring Environmental and climatic behaviour observation PHOTON STARVING APPLICATIONS Exoplanet transits Lucky imaging Adaptive optics High-resolution spectroscopy Fabry-Pérot imaging spectroscopy Integral field spectroscopy Real-time photometry 31
36 À PROPOS DE NÜVÜ CAMÉRAS 32
37 TRAVAIL D ÉQUIPE 15 employees in May 2015 founded in 2010 by Marie-Eve Ducharme (CEO) and Dr Olivier Daigle (CTO) more than a decade of Research & Development technology 100% designed and made in Canada CCD Controller for Counting Photons patented technology unique EMCCD camera packaging deserving the global market 33 NUVU CAMERAS INC 2015
38 TRAVAIL D ÉQUIPE 2011 Regional Award for New Technology granted by Canadian Manufacturers & Exporters (CME) and the National Research Council of Canada Total of 4 prizes at the 13th annual Québec Entrepreneurship Contest in 2011 including the GURU Entrepreneur Grand Prize Dr Olivier Daigle was named Personality of the Year 2011, category Pure Sciences & Technology by La Presse & Radio-Canada Dr Olivier Daigle was awarded the 2009 Discovery of the Year by Québec Science magazine Extensive local and international media coverage 34 NUVU CAMERAS INC 2015
39 CONCLUSION COMPAGNIE INNOVANTE AVEC UNE TECHNOLOGIE UNIQUE SUR UN MARCHÉ MONDIAL COLLABORE ÉTROITEMENT AVEC SES CLIENTS PRIORITÉS AU NIVEAU SCIENTIFIQUE, PUBLICATIONS ET CONFÉRENCES 35
40 QUESTIONS? NUVU CAMERAS INC
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