ELITE Custom Antibody Services

ELITE Custom Antibody Services

ELITE Custom Antibody Services Experience, confidence, and understanding

As a manufacturer and service provider, we have the experience, confidence, and understanding to develop an antibody that meets your specific requirements. From our polyclonal antibody services through high throughput clone screening, let us put our expertise to work for you.

Overview of Antibody Development Workflow Antigen selection and design Peptide purification & conjugation Immunization Primary Screen Delivery Validation & optimization Antibody purification Secondary screen

Antigen selection and design Abgent has vast experience with antigen selection and design we ll work with you to develop the antigen best for your antibody target and application/s. Compounds Custom designed peptides Recombinant proteins Whole cells

Immunization Whether it s our improved immunization process (below) or your customized immunization protocol, we ll work with you to reduce project time and improve project success rate. A novel approach to adjuvant preparation and immunization Current immunization protocols involve a greater than two month process, tedious adjuvant preparation, undue stress on the animals, and a relatively low success rate. Compared with the conventional Freund s adjuvant, our new procedure allows for fewer immunizations, a reduced antigen dosage, and quick antibody induction. Resulting antibodies show a high titer and high affinity. Our new process does not alter the antigen conformation and is easy to use.

Screening We ll work to screen, identify, and validate rare clones. Our project team will suggest the screening process best for your specific antibody requirements. We have 300 cell lines and abundant cynomolgus and human tissues. Primary Screening: High capacity ELISA High capacity flow cytometry Secondary screening: Western Blot Immunofluorescence Immunohistochemistry Functional assay Customer s preferred assay

Validation and optimization With over a decade of experience developing 20,000 antibodies for research and drug discovery, we understand your application/s requirements. We ll deliver a validated antibody optimized for your specific application/s. Immunofluorescence Flow cytometry Antibody labeling Immunohistochemistry Immunoprecipitation Scale up

Validation and optimization Our validation approach has the highest Quality Standard. By choosing proper cell lines and tissues, and applying correct experimental design we assure that your antibody has biological and functional significance LC3 Antibody (APG8B) (N-term) TTR Antibody (C-term)

Basic ELITE Custom Service Packages Abgent offers basic packages for monoclonal and polyclonal antibody development. These packages optimize project delivery time by offering limited customization. More complex customization is available based upon you specific needs.

Modification-state Antibodies We ll work with you, from antigen design through scale up, to deliver antibodies validated and optimized for your application/s of interest. Methylation and acetylation events are essential steps in epigenetic gene activation/silencing. These modifications are increasing in importance within drug discovery Phosphorylation plays a critical role in signaling cascade Many oncology drug discovery targets are known to be active/inactive upon phosphorylation event

ELITE Custom Services for Drug Discovery We understand the diversity of your antibody needs. Because of this, we offer an industry leading range of custom antibody services. HTS ELISA screen Flow cytometry based high throughput phenotypic screening Biochemical characterization of antibody Recombinant antibody production Cell line generation and cell based assays for validation Sandwich ELISA antibody pairing Antibody sequencing

ELITE Services for Drug Discovery Recombinant Antibody Production Platform mrna isolation, cdna and gene synthesis Transfection to CHO or 293 cells In case that you may require scale or further antibody optimization, we suggest recombinant monoclonal antibody production. We have had great success developing recombinant antibodies to allow for scale, consistency, and quality. Recombinant antibody purification kd 150 80 58 45 Marker Supernatants Purified Herceptin 1 2 5 10 μg Heavy chain 30 25 Light chain Functionality assessment Reduced 15% SDS-PAGE of Herceptin

ELITE Services for Drug Discovery High Capacity ELISA Clone Screening Platform To increase project success by identifying rare clones, we offer high-throughput clone screening using ELISA with a primary screening capability of 10,000 to 50,000 samples per assay.

ELITE Services for Drug Discovery High Capacity Flow-based Screening Platform The Intellicyt Screener a revolution in screening in solution. Flow-cytometry based, high-throughput, phenotypic screening of suspension cells can now be performed by Abgent. For antibody discovery studies, the ability to acquire both antigen binding and cross-reactivity simultaneously allows for the critical collection of data. Moreover, targets can now be screened in the context of the cell.

ELITE Services for Drug Discovery FACS (Fluorescence-activated cell sorting ) and MACS (Magnetic-Activated Cell Sorting ) services Measure cell surface markers by FACS Cell sorting by MACS Splenocyte Cell based assays B B MACS Resting B cell B B

ELITE Services for Drug Discovery HTS cell-based & beads-based assay by Intellicyte High throughput and Multiplex Cell based assays : compound screening, compound characterization, and toxicology evaluation Bead based assays : compound screening and phamaco-dynamic assessment

ELITE Services for Drug Discovery The Acumen Explorer microplate cytometer offers researchers a flexible, high-throughput, high-content screening approach for many biochemical screens. Acumen ex3 Cell cycle analysis by Acumen

ELITE Services for Drug Discovery additional services Cell line generation and cell based assays for validation Antibody sequencing Surface Plasmon Resonance ELISA antibody pairing Biochemical characterization of antibody ChIP

How ELITE Service Fits into Drug Discovery Pipeline Anywhere an RUO custom antibody is needed in the drug discovery and development process or companion diagnostics

Summary of Applications Areas Where ELITE Custom Services Can Be Applied

Conclusion Experience, Confidence and Understanding Unsurpassed hybridoma screening capabilities allows for identification of rare clones and increased project success rate. Comprehensive validation and optimization in customer-defined applications ensures that the antibody will work the first and each time used in your specific application. 300 ATCC cell lines; abundant mouse, rat, rabbit, cynomolgus and human tissues. Rapid turnaround time leveraging our experience developing over 20,000 antibodies. Project support from industry veterans ensures rapid and concise communication. Abgent s animal facilities with recognized AAALAC credentials.

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