PperCHIP. High-Content Peptide Microarrays. Epitope Mapping & Serum Profiling Services

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1 PepperChip High-Content Peptide Microarrays PepperMap Epitope Mapping & Serum Profiling Services PperCHIP access to custom and dard peptide microarrays up to 9,000 features per in a uniquely flexible and -effective manner.

2 PepperChip PEPperCHIP Specifications custom and off-the-shelf peptide microarrays up to 9,000 features per chip thorough customization covering any protein or peptide collection fully adjusted to your research standard slide formats with wide chip reader compatibility GenePix Pro and PepSlide Analyzer support most cost effective solutions PEPperCHIP - PEPperPRINT s new peptide microarray platform with custom and standard contents PEPperCHIP Variants Benefit from custom contents adjusted to your research: display your antigen(s) as overlapping peptides for epitope mapping use more than 4,100 random peptides for mimotope discovery and serum signaturing decode known peptides and epitopes with full permutation scans translate custom peptide lists into peptide microarrays include multiple array copies per chip for one-by-one tests Custom solutions from single antigens to full proteome sets. numerous standard contents available (antigens from EBV, HBV, H1N1, HCV, Dengue virus, HPV, melanoma etc.) PEPperCHIP Accessories PEPperCHIP Incubation Trays with 3/1, 3/2, 3/3, 3/4, 3/5, 3/16 and 3/(3,4,5) well formats 33one-by-one assays with multiple array copies 33minimal sample consumption 33enables shaking incubation PEPperCHIP Staining Kit with labeled anti-ha and anti-flag antibodies for control assays PepSlide Analyzer for software-assisted image analysis of peptide microarray data GenePix Pro support with GAL files PEPperCHIP Incubation Tray (3/4 well format)

3 PepperChip Applications Epitope Mapping characterization of mono- & polyclonal antibodies and sera proteins are translated into overlapping peptides with maximum peptide-peptide overlap custom peptide mapping contents from single to multiple antigens variable peptide lengths and overlap Epitope mapping of a polyclonal antibody against two antigens as overlapping peptides in double spots (red; stained control peptides frame the antigen-derived peptides) up to five one-by-one tests per chip possible minimal sample consumption Immune Profiling of Blood Sera fingerprint analysis of immune responses in sera linked with infection, vaccination, oncogenesis, autoimmune diseases identification of differential signatures in patient and control groups custom and off-the-shelf contents available covering various indications, organisms, strains, subtypes and antigen collection high reproducibility and data consistency for statistical analysis of microarray data material saving with suited PEPperCHIP Incubation Trays Custom peptide microarrays with more than 8,000 features for the discovery of serum signatures (left) and full permutation scans of a known antibody epitope (right) Antigen/Epitope/Mimotope Discovery Access high content microarrays with up to 25,000 peptides for the discovery and development of: new epitopes and mimotopes serum biomarker and antibody signatures active antigens in complex protein collections protein target binders and affinity tags peptide hit discovery and lead optimization High content PEPperCHIP with 25,000 peptides for antigen, epitope, mimotope and target binder discovery (right)

4 PepperMAP Services PEPperMAP Specifications Benefit from PEPperPRINT s state-of-the-art peptide microarray platform PEPperCHIP without any need for time, manpower or technical infrastructure. Just send us your antibody or serum sample and let PEPperPRINT s experts get the results for you. We are pleased to provide you with standard epitope mapping services and custom serum profiling solutions for your research. PEPperMAP - The most convenient way to benefit from PEPperPRINT s new peptide microarray platform. Epitope mapping of a monoclonal antibody with a single epitope (left) compared to a polyclonal sample (bottom) with two epitopes. PEPperMAP Elements a custom peptide microarray with your antigen as overlapping peptides all immunoassays with your antibody or serum sample and secondary antibodies control assays of Flag and HA control peptides read-out of microarray data data quantification and evaluation Green/red frames: Staining of routinely implemented HA and Flag control peptides. detailed report with protocols, experimental details and results PEPperMAP Options According to your demand, we offer various epitope mapping options from only 675 on as well as completely customized PEPperMAP solutions. Type 1 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 13mer peptides with a peptide-peptide overlap of 12 amino acids Type 2 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 10, 12 and 15mer peptides with a peptide-peptide overlap of 9, 11 and 14 amino acids Type 1+/2+ Mapping with success-related fees Type 3 Mapping: Full epitope scans with thorough sequence permutations Please inquire or download our Product Catalog ( PEPperPRINT_Catalog.pdf) for a comprehensive product overview and pricing information.

5 PepSlide Analyzer PepSlide Analyzer Specifications Quantify and analyze your peptide microarray data with our intuitive and easy-to-use software solution: use microarray scanners TIFF images and load design data from GAL files or PepSlide PSF files efficient tools for image processing, array alignment and spot finding perfectly adapted to the PEPperCHIP platform full compatibility with Excel recognition of custom spot sizes and morphologies state of the art background correction cost-effective alternative to GenePix Pro Custom Peptide Synthesis Peptide Specifications HPLC purified custom peptides (95% purity on demand) 1 mg ( 11/amino acid), 5 mg ( 22/amino acid) and 10 mg ( 33/amino acid) batches 16, 96 and 384-well small scale peptide libraries linear and cyclic peptides various modifications available (phosphorylation, PEGylation, biotinylation, acetylation, methylation etc.) PEPperCHIP useful for competition and enzymatic assays, peptide Get access to custom and immobilization and peptide array spotting standard peptide microarrays with up to 9,000 features per Please inquire for other peptide purities, quantities and modifications. chip in a uniquely flexible and cost-effective manner.

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