SCIENCE WHERE YOU ARE

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1 Newsletter. November 2013 SCIENCE WHERE YOU ARE Dealing with BioNordika includes a number of first class benefits: Next day delivery.... and some times even faster! Free delivery. Focus on your application. Instant technical support. Personal customer service. High quality products and suppliers.

2 Dear customer This edition will primarily focus on antibodies for different applications from the leading manufactures such as Cell Signaling Technology, Rockland and Eurogentec. We are also pleased to introduce Vector Laboratories as our new supplier of innovative labeling and detection systems for IHC and IF. In the following pages you can also find great discounts on several new products. Our team is always ready to help you make the right choice of products for your research. We look forward to a great cooperation. On behalf of BioNordika Denmark A/S. Heinrich Biehl CEO SOLUTIONS FOR LIFE SCIENCE BioNordika provides a wide range of innovantive and high quality solutions for your research. Cloning systems. PCR/qPCR systems. Next generation sequencing. Antibody detection systems. Custom made production. Expert technical support, delivery and service.

3 HOT NEWS! New supplier at BioNordika! We are pleased to inform you that it is now possible to buy products from VECTOR LABORATORIES here at BioNordika. Vector Laboratories is an expert in labeling and detection systems. They revolutionized the field of histological staining with development of the versatile and sensitive Biotin-Avidin detection method leading to the VECTASTAIN ABC family of products. These reagents provide consistent, reliable, sensitive, and low background staining for immunohistochemistry and other applications. Latest product news! Vector ImmPRESS Polymer Reagents Now available with Alkaline Phosphatase. - ImmPRESS-AP Anti-Mouse Ig (alkaline phosphatase) Polymer Detection Kit (Cat. No. MP-5402). - ImmPRESS-AP Anti-Rabbit Ig (alkaline phosphatase) Polymer Detection Kit (Cat. No. MP-5401). Vector Mouse on Mouse (M.O.M.) ImmPRESS Peroxidase Polymer Kit - Mouse on Mouse (M.O.M.) ImmPRESS Peroxidase Polymer Kit. (Cat. No. MP2400)

4 Offer valid until 31st December 2013 The XP Benefit + exceptional Specificity. + exceptional Sensitivity. + exceptional Stability and Reproducibility. = exceptional Performance! One antibody - Multiple applictions XP monoclonal antibodies are a line of high quality rabbit monoclonal antibodies exclusively available from Cell Signaling Technology (CST). Any product labeled with XP has been carefully selected based on superior performance in the most relevant research applications. PI3K/Akt Signaling, using the XP mab Phospho-Akt (Ser473) #4060 Buy 2 L-size Abs and get -25% on the cheapest one*. XP monoclonal antibodies are generated using XMT technology, a proprietary method developed at CST. This technology provides access to a broad range of antibodies unattainable with traditional monoclonal technologies, allowing more comprehensive screening and the identification of XP monoclonal antibodies. MAP Kinase Signaling using the XP mab Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) #4370 *Use the code CST25 when ordering. Can not be combined with other discounts.

5 Conjugated Antibodies for IF Imaging. November 2013 Cell Signaling Technology conjugates Alexa Fluor dyes with our highest quality antibodies to give you the brightest signal and lowest background. Conjugation procedures are carefully adapted in-house for each antibody to yield the best fluorescent imaging. Alexa Fluor Alexa Fluor 488, 555, 594, 647 dyes. Brighter fluorescence output than similar fluorochromes, e.g. FITC, Cy3, Texas Red -X or Cy5. Highly photostable. Spectrum covered by most fluorescence microscopes. CST Conjugated Antibodies Optimized in-house conjugation procedure for each antibody. Tested and validated for fluorescent imaging research. Primary and secondary conjugated antibodies available. Convenience of one-step staining. Alexa Fluor conjugated secondary antibodies are F (ab )2 fragments, which exhibit reduced background staining. Offer valid until 31st December % on products below* XP Monoclonal Antibodies - Top Targets for IF Number Name Validated Applications Species #4060 Phospho-Akt (Ser473) (D9E) XP Rabbit mab W IP IHC-P IHC-F IF-IC F H M R Hm Mk Dm Z B (C) (X) (Dg) (Pg) #8768 Stat3α (D1A5) XP Rabbit mab W IP IHC-P IF-IC ChIP H M R Hm Mk (Pg) #5018 Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP Rabbit mab (Alexa Fluor 488 conjugate) IF-IC F H M R Mk #12741 LC3A/B (D3U4C) XP Rabbit mab W IHC-P IF-IC H M R (Mk) (X) (B) (Dg) (Pg) #4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D E) XP Rabbit mab W IP IHC-P IF-IC F H M R Hm Mk Mi Dm Z B Dg Pg Sc (C) (Ce) #2914 Phospho-Aurora A (Thr288)/Aurora B (Thr232)/ Aurora C (Thr198) D13A11) XP Rabbit mab W IF-IC F H M R *Use the code IF20 when ordering. Can not be combined with other discounts.

6 Offer valid until 31st December 2013 TrueBlot Immunoprecipitation, Western Blot and IP Western Obtain higher resolution data and compromise elsewhere. * TrueBlot products Cat. no. Rabbit TrueBlot Western Blot Kit Mouse Trueblot Western Blot Kit Goat TrueBlot Western Blot Kit Use the code ROCK35 when ordering. Can not be combined with other discounts. Immunoprecipitation protocols and Western Blots provide highly specific results, yet often suffer from heavy/light chain blotting, contamination, and ongoing interference. Intro price -35% on selected TrueBlot products* TrueBlot products solve nearly all of these problems through increased sensitivity, less background noise, and enhanced accuracy. TrueBlot reagents enable you to generate clear, best-quality data in individual Immunoprecipitation and Western Blots (Nfkb Western Blots as well as GFP Immunoprecipitation Western Blots), but can also be used when the two protocols are combined. TrueBlot is referenced in over 300 publications! ( Rockland secondary antibodies Can quickly and accurately detect, sort, and purify target antigens based on your specifications. Choose from a very broad range of species and conjugates for different applications. IRDye Secondary Antibodies HRP Secondary Antibodies ATTO Dye Secondary Antibodies

7 Speedy 28-day The reference for custom polyclonal antibodies designed to meet you stringent requirements in record time! Just 28 days instead of the standard 87 days. What is included? Speedy 28-day Protocol Free ELISA at day 21 for each anti-peptide program (Goat immunizations and Epiproteomics programs excluded). Peptide design from your antigen sequence. Peptide Synthesis (>70%, mg, 16 aa) and peptide coupling (KLH, BSA, OVA). 2 Rabbits, Guinea pigs or Goat. 28-day immunization protocol. All immunizations, blood collections and animal care. Delivery of free remaining peptide. A single shipment on dry ice. Speedy packages Antigen Host Reference Your antigen Rabbit (2 animals) AS-SUPR-ANTIGEN Guinea pig (2 anim.) AS-SUPR-GPANTIGEN Rat (2 animals) AS-SUPR-RATANTIGEN Goat (1 animal) AS-SUPR-GOATAG Prices from DKK 8574,- Per production 1 peptide Rabbit (2 animals) AS-SUPR-SINGLE Guinea pig (2 anim.) AS-SUPR-GPSINGLE Rat (2 animals) AS-SUPR-RATSINGLE Goat (1 animal) AS-SUPR-GOATSING DoubleX- 2 peptides Rabbit (2 animals) AS-SUPR-DX Guinea pig (2 anim.) AS-SUPR-GPDX Rat (2 animals) AS-SUPR-RATGX Goat (1 animal) AS-SUPR-GOATDX DoubleXP-2 peptides + Rabbit (2 animals) AS-SUPR-DXP affinity purification Guinea pig (2 anim.) AS-SUPR-GPDXP Rat (2 animals) AS-SUPR-RATDXP Goat (1 animal) AS-SUPR-GOATDXP Phospho-specific Rabbit (2 animals) AS-SUPR-PSPEC

8 SOLUTIONS FOR LIFE SCIENCE BioNordika provides a wide range of innovate and high quality products for your research including next gen seq solutions, cloning systems, restriction enzymes, PCR reagents, antibodies incl. custom production, biochemicals, and much more. Our selection of suppliers include:

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