MAB Solut. MABSolys Génopole Campus 1 5 rue Henri Desbruères Evry Cedex. is involved at each stage of your project
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1 Mabsolus-2015-UK:Mise en page 1 03/07/15 14:13 Page1 Services provider Department of MABSolys from conception to validation MAB Solut is involved at each stage of your project Creation of antibodies Production & Purification Bespoke modification In vitro/in vivo characterization MABSolys Génopole Campus 1 5 rue Henri Desbruères Evry Cedex phone : +33 (0) contact@mabsolut.com
2 MAB Solut, the R&D department services provider of MABSolys, a major player in the antibody area, specializes in bespoke antibodies R&D. MAB Solut Customers Our services are dedicated to companies and laboratories which want to develop antibodies against specific targets for therapeutics, diagnosis or research tools, such as: Research laboratories Pharmaceutical and biotechnology industries Professionals in diagnostics and theranostics fields MAB Solut offers global and customized solutions, which will enable the achievement of your projects. We play a crucial role in antigen engineering up to the in vitro characterization of antibodies as well as in completion of toxicological tests and efficacy validation on animals. All MAB Solut services are provided with an accurate and detailed report focusing on the set up for the completion of your project as well as on the characteristics of your deliverables. MAB Solut leads your projects in a highly confidential way and in accordance with the Industrial Property rules.
3 Antibodies Creation Tailor made creation Preliminary phase Specifications In order to better meet your requirements, for each project, we precisely define together the specifications, based on: the type of target against which you want your antibodies to focus on the final applications of your project the specific constraints related to the future use of your product Target set up and validation We prepare the target (virus, bacteria, proteins, peptides, chemical compounds) taking into account its nature and its immunogenic properties (hapten conjugation, fragmentations...). Antibody development steps Immunization Injection of antigens according to standard or specific protocols Evaluation of the response by titration of serum Fusion Screening Extraction of spleen cells Fusion of cells with a mouse myeloma cell line Cell culture Boost of the response if necessary Recovery and titration of serum Polyclonal antibodies Primary screening tests of supernatants in a screening plate Hybridomas Cloning Selection of hybridomas producing antibodies Cloning by limiting dilution of the positive hybridomas Screening of supernatants in screening plates Selection of hybridoma cell lines secreting monoclonal antibodies Monoclonal antibodies produced by hybridomas MABSolys - Genopole Campus 1-5, rue Henri Desbruères Evry Cedex - contact@mabsolut.com Phone : +33 (0)
4 Int_2_def_UK:Mise en page 1 03/07/15 14:33 Page1 Production & purification Production & purification of antibodies Production MAB Solut offers different in vitro production methods (in high or low density) depending on the criteria defined during the set-up of the specifications: Intrinsic properties of the hybridoma Production scale expected In vitro Production scale 1mg Culture in flasks (Routine production) 100mg 2g Research step (Blot, ELISA, immunohistochemistry cytometry)) Culture in CELLineTM and in HYPERflaskTM (high density production) 10mg Development step (tests, in vivo studies, validation process) Purification MAB'Solut implements, in its FPLC equipment, different methods of purification for your antibodies, based on: their physico-chemical properties their isotype their origin Main purifications methodologies used - Affinity chromatography (peptide, protein A, protein G, protein L, target protein) nal Monoclo Antibodies - Thiophilic adsorption (used for IgM) MABSolys - Génopole Campus 1-5, rue Henri Desbruères Evry Cedex - contact@mabsolut.com Phone : +33 (0)
5 Bespoke modification Bespoke monoclonal antibodies modification MAB Solut offers numerous options to modify your antibody, in order to obtain efficient solutions which will be useful in all your projects and applications. Antibody engineering Conjugation Conjugation or labelling an antibody with a molecule of interest can be made with different kinds of molecules, as for example biotin, enzymes, fluorochromes or cytotoxic molecules. Chemical fragmentation Two types of chemical fragmentation can be made: Fragmentation by papain or ficin, which enables to cut and release the two Fab fragments Fragmentation by pepsin or ficin, which enables the selection of the F(ab)' 2 fragment { } Fab cleavage site papain / ficin cleavage site F(ab)' 2 pepsin / ficin Molecular biology MAB Solut also offers other tailor-made services for your antibodies: Mutagenesis (construction of mutant data library) Genes synthesis (codons optimization, Fab fragments ) Subcloning into prokaryotic or eukaryotic expression vectors Transient transfection or development of stable cell lines Chimerization / humanization MABSolys - Genopole Campus 1-5, rue Henri Desbruères Evry Cedex - contact@mabsolut.com Phone : +33 (0)
6 in vitro/in vivo characterization Characterization tests MAB'Solut offers a panel of tests to characterize your antibodies, standardized or tailor-made, to meet your requirements (note that for oncology applications, we provide several established animal models). In vitro characterization Analytical process Affinity, isotyping, specificity (western blot, SDS PAGE, ELISA, cytometry ) Sequencing (light and heavy chains) Functional process Apoptosis Differentiation Proliferation ADCC/CDC Migration Bespoke immunoassay development Preliminary study and development (feasibility study, choice of strategy for the test development) ELISA (direct, indirect, competitive), cytometry Validation (limit of detection / quantization, precision, robustness, reproducibility, specificity, etc.) In vivo characterization Efficacy studies in animals models (especially in oncology - with xenograft models - and in autoimmunity) Toxicity / anatomopathology studies For any other requests regarding the development of proof of concept to test the efficacy of your antibodies on animals, please contact us; our team of scientists will bring you their expertise. MABSolys - Genopole Campus 1-5, rue Henri Desbruères Evry Cedex - contact@mabsolut.com Phone : +33 (0)
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