GenScript Antibody Services

Transcription

1 GenScript Antibody Services Scientific experts, innovative technologies, proven performance We specialize in custom antibody production to empower your research Custom Polyclonal Antibody Production Custom Monoclonal Antibody Production Phospho-Specific Antibody Production Antibody Drug Development

2 GenScript Technology Advantage Op muman gen TM design tool, powered by the industry s most advanced an gen design algorithm, designs superior pep des for an body produc on ImmPlus TM an gen modifica on technology to break immune tolerance to produce surrogate an bodies Proprietary adjuvant technology to elicit stronger and faster cellular immune reponse and longer immune stability, making produc on of high- ter, high-affinity an bodies possible GANP transgenic mice are licensed from Transgenic Inc. and allow for produc on of high-affinity and high-specificity an bodies through overexpression of Germinal Center Associated Nuclear Protein (GANP) At GenScript, our team of scientists possess unparalleled expertise in custom antibody and drug development. Since 2004, we have provided over 13,000 high quality custom antibodies to clients all over the world. Our streamlined process takes you from gene sequence to antigen design and synthesis, antibody development, assay development, and beyond. Partnering with GenScript ensures that you will always benefit from: Advanced technologies Rigorous quality control Scientific experts offering support and advice throughout your project Fast turnaround time State-of-the-art animal facility (AAALAC International accredited and OLAW assured animal facility) Flexible customization options Competitive pricing 1

3 One-Stop Solution for Antibody Production PolyExpress TM polyclonal antibody Standard polyclonal antibody Custom Polyclonal Antibody Production Custom Monoclonal Antibody Production MonoExpress TM monoclonal antibody Customized monoclonal antibody MamPower TM recombinant monoclonal antibody FragPower TM antibody production Phospho-specific polyclonal antibody Phospho-specific monoclonal antibody Phospho- Specific Antibody Production Antibody Drug Development DNA immunization Ab development Anti-idiotype Ab production Premium hybridoma development HT Gene to Ab Ab library & phage display The GenScrlpt logo resembles a drop of water and symbolizes the source of life. It inspires our dedication to evolve with our customers and push the limits of technology to save more lives by providing early drug discovery and development. Founded in 2002, GenScript has grown together with the synthetic biology industry and is now the world's largest gene synthesis supplier. Headquartered in New Jersey with subsidiaries in Europe, Japan, and China, GenScript consistently provides the best research to the scientific community worldwide. Built on our assembly-line mode, one-stop solutions, continuous improvement, and stringent IP protection, GenScript provides a comprehensive portfolio of. Besides our world-renowned gene synthesis service, GenScript offers comprehensive for biological research and early-phase drug discovery, such as bio-reagents, agricultural research, food industry research, assay development & screening, antibody drug development and research, and animal model. With an impressive track record of success, GenScrlpt is your ideal, reliable innovation partner in advancing and transforming biology research to make your research easy. 2

4 GenScript Antibody Production Services Peptide vs. protein antigen selection based on application Blot IHC ELISA IP/ChIP Sandwich ELISA IF/Flow cytometry Functional Assay Linear epitope peptide antigen acceptable protein antigen preferred Folded epitope Antibody Service Selection Guide Polyclonal Ab Monoclonal Ab Recombinant protein or peptide Others Recombinant protein or peptide Others PolyExpress TM Ab antigen MonoExpress TM Ab antigen No Standard packages No Anti-idiotype No Custom packages Anti-idiotype Custom packages What to expect from our process Submit protein sequence Antigen design by sophisticated design program Peptide or protein antigen synthesis Ab production and purification QC by WB/ELISA Delivery of final product ELISA Western blot IHC IP FACS Others 3

5 Highlights of Our Major Antibody Services Express 45-day Ab service packages Anti-idiotype and DNA immunization Ab development Fully customizable options Quantity, purity, and quality guarantees Custom Polyclonal Antibody Production PolyExpress TM Fast pab Services PolyExpress TM Antibody Packages offer an unprecedented guarantee to our customers ensuring delivery of 1-5 mg of purified antibody with a final ELISA titer of at least 1:64,000. With our Silver and Gold packages delivering more pab for your target protein, you can stretch your research dollars, further increasing your opportunity for success by 2-3 times over other commercial pabs. Delivery comes at record-breaking speed in only 45 days (from order to delivery), faster than any other vendors and at the competitive price of only $1,099 for 2 custom antibodies. All of this comes with a free-if-fail guarantee that eliminates the risk to you. This package cannot be replicated by others. PolyExpress TM Rabbit Polyclonal Antibody Packages* Package Antigen Antibody Production Features Deliverables PolyExpress TM Premium SC1676 Recombinant protein** to make antibody 1 Ab generated against protein WB detection of immunogen protein guaranteed 1-5 mg purified Ab PolyExpress TM Gold SC peptides to make antibodies 3 Abs generated against 3 individual epitopes WB detection of native protein success >95% 3 affinity-purified Abs (1-5 mg each) PolyExpress TM Silver SC peptides to make antibodies 2 Abs generated against 2 individual epitopes WB detection of native protein success >85% 2 affinity-purified Abs (1-5 mg each) PolyExpress TM Basic SC peptide to make antibody 1 Ab generated against 1 epitope Thousands of successful cases 1-5 mg purified Ab *Silver and Gold packages only apply to a single target protein sequence. Cannot be combined from different protein targets. **Protein may be full length or a protein fragment 10 KD depending on which is more likely to generate a successful antibody. 4

6 Standard pab Services* Complete Affinity-Purified Pep de Supplied pab Package (Rabbit) SC1031 Customer Provides Deliverables Delivery Time An gen sequence mg affinity-purified an bodies (ELISA ter of 1:64,000) 5 mg unconjugated pep de 1-2 ml pre-immune serum Cer ficate of Analysis report, MS and HPLC reports Total: 13 weeks Pep de Supplied pab Package (Rabbit) SC1015 Customer Provides Deliverables Delivery Time An gen sequence ml an sera from 2 rabbits (ELISA ter of 1:64,000) 5 mg unconjugated pep de 1-2 ml pre-immune serum Cer ficate of Analysis report, MS and HPLC reports Total: 16 weeks Protein A-Purified Pep de Supplied pab Package (Rabbit) SC1045 Customer Provides Deliverables Delivery Time An gen sequence mg protein A-purified an bodies (ELISA ter of 1:64,000) 5 mg unconjugated pep de 1-2 ml pre-immune serum Cer ficate of Analysis report, MS and HPLC reports Total: 13 weeks Phospho-Specific pab Package (Rabbit) SC1044 Customer Provides Deliverables Delivery Time An gen sequence 1-30 mg phospho-specific an bodies from 2 rabbits (ELISA ter of 1:64,000) 5 mg phospho-pep de 5 mg non-phospho-pep de 1-2 ml pre-immune serum Cer ficate of Analysis report, MS and HPLC reports Total: 17 weeks *Produc on from mice, rats, chickens, or goats/sheep also available 5

7 Custom Monoclonal Antibody Production MonoExpressTM Fast mab Services MonoExpress custom monoclonal antibody production allow you to choose either peptide or recombinant protein as antigen. You supply the protein sequence and we will deliver your specific hybridoma supernatants within 45 days of antigen preparation. MonoExpress Silver and Bronze custom monoclonal antibody production packages use peptide antigen and each starts with peptide antigen design using our proprietary OptimumAntigen design tool, followed by peptide synthesis, mouse or rat immunization, all the way to final specific hybridoma screening and selection. MonoExpress Gold and Basic packages use protein antigen: the Gold package includes protein antigen production, while the Basic package requires you to provide your own protein antigen. In addition to the delivery of specific hybridomas, both packages also deliver 2 mg affinity-purified antibody with Western Blot guarantee. MonoExpressTM Monoclonal Antibody Packages Package Antigen MonoExpress Premium SC1711 TM MonoExpressTM Gold SC1710 MonoExpressTM Silver SC1661 MonoExpressTM Bronze SC1660 MonoExpressTM Basic SC1717 MonoExpressTM Phospho-specific SC1659 * Recombinant protein to make antibody Antibody Production Up to 5 Ab-producing hybridomas 3 peptides to make antibodies 6 Ab-producing hybridomas 2 peptides to make antibodies 4 Ab-producing hybridomas 1 peptide to make antibody 2-5 Ab-producing hybridomas Customer s protein antigen to make antibody Up to 5 Ab-producing hybridomas Using 1 peptide to make antibody 2-5 Ab-producing hybridomas Guarantees At least one clone works for customer's application & ELISA titer of 1:64,000 Deliverables First: 10 supernatants for evaluation** Final: Up to 5 hybridomas, 2 mg purified Ab, 200 µg immunogen protein ELISA positive for immunogen 6 hybridoma cell lines (2 clones/ peptide) 6 supernatants (5 ml/clone) 2 mg unconjugated peptide ELISA positive for immunogen 4 hybridoma cell lines (2 clones/ peptide) 4 supernatants (5 ml/clone) 2 mg unconjugated peptide ELISA positive for immunogen 2 hybridoma cell lines (2 clones/ peptide) 2 supernatants (5 ml/clone) 2 mg unconjugated peptide WB positive for the immunogen and ELISA titer of 1:64,000 <10% cross-reactivity with non-phosphopeptide First: Up to 5 supernatants Final: Up to 5 hybridomas, 2 mg purified Ab First: 2-5 supernatants, 2 mg phosphopeptide, 2 mg non-phosphopeptide* Final: 2-5 hybridomas, 2 mg purified Ab <15aa and 90% purity If customer cancels the project after evaluating supernatants, they will only be charged for the first deliverables, which is $1,000. ** 6

8 VL VH CL CH Customized Monoclonal Antibody Package Service Options Deliverables Phase I Immunization Conventional protocol or MonoExpress TM protocol Animals: Balb/c mice, C57BL6 mice or rats Number of animals Test bleed report or samples Phase II Cell fusion and Screening Conventional indirect ELISA screening and WB validation with immunogen Capture ELISA, Competitive ELISA, FACS screening, or Counter screening Additional screening option available Parental supernatant report or samples sent to client. Client may select best candidates to enter Phase III Phase III Subcloning, Screening, and Expansion Hybridoma cell lines Recombinant Antibody Production Services MamPower TM Recombinant Monoclonal Antibody Production Service SC1259A Features Deliverables Timeline When to use this service Codon optimization & gene synthesis Guaranteed service Synthetic gene 50 mg purified antibody QC data 8-12 weeks When you need hassle-free, high quality, synthetic gene and guaranteed transient mammalian protein expression FragPower TM Guaranteed Antibody Fragment Services Fragment Expression Amount Purity Timeline Deliverables VL VH scfv E.coli 10 mg 90% 7 weeks VH V HH /V H E.coli 10 mg 90% 7 weeks Fab Insect 3 mg 90% 10 weeks Ab fragment in buffered solution Optimized gene sequence report QC data 7

9 Post-Translational Modification-Specific Antibody Production Phospho-Specific Monoclonal and Polyclonal Antibody Packages Phospho-Specific Antibody Packages Packages You Provide Deliverables and Guarantees Timeline Polyclonal Phospho-specific Antibody Package SC1044 Target antigen/protein sequence Final Deliverables 1-30 mg purified antibody 5 mg phosphopeptide* 5 mg non-phosphopeptide* 1-2 ml of pre-immune serum ELISA results** 17 weeks Guarantees ELISA titer of 1:64,000 and <10% cross-reactivity with non-phospho peptide Monoclonal MonoExpress TM Phospho-specific Antibody Package (mouse or rat) SC1659 Target antigen/protein sequence First Deliverables 2-5 supernatants Final Deliverables 2-5 hybridoma cell lines 2 mg purified Ab 2 mg phosphopeptide* 2 mg non-phosphopeptide ELISA results** First Delivery weeks Guarantees 2-5 hybridomas and at least 1 with ELISA titer of 1:64,000 and <10% cross-reactivity with non-phosphopeptide Custom pab or mab phospho-specific package Your own physical antigen Varies with custom package design Varies with custom design Other modification-specific antibodies are also available upon request * <15aa and 90% purity ** both for phosphopeptide and non-phosphopeptide, MS and HPLC reports Our customers have used our antibody products and to publish in Journals including: Nature EMBO PNAS J. of Am Med Assoc Mol Biol Cell Cancer Research Journal of Cell Science Journal of Bio Chemistry Plant Cell Nature Commun 8

10 Drug Development Hybridoma Development Sequencing in vitro Assay and Screening /Protein Characteriza on Chimeric Genera on Produc on Drug Development Humaniza on Stable Cell Line Affinity Matura on Transient Expression Epitope Mapping in vivo Efficacy and Safety This one-stop service package starts with Antigen Production and Hybridoma Development, and is followed by Antibody Sequencing, which provides the basis for antibody optimization. In addition, Chimeric Antibody Generation offers a fast and inexpensive approach for generating therapeutic antibodies, and Antibody Humanization provides a more advanced way of reducing immunogenicity of antibodies to human antigens. Affinity Maturation gives your antibody desired affinity to achieve its function. Epitope Mapping not only provides important information on the nature of your antibody, but also facilitates patent application to protect it. Properties of your antibody and antigen such as affinity and thermostability can be measured through Antibody/Protein Characterization. Biological functions and activities of your antibody and antigen can be assessed via in vitro Assay and Screening as well as an in vivo Efficacy and Safety study. At the end, Antibody Transient Expression, Antibody Stable Cell Line and Antibody Production produce sufficient amount of antibodies for your research and development project. Drug Development Therapeu c Hybridoma Engineering Bio-Assay Animal Model Bio-process An gen produc on Immuniza on in vivo transfec on Cell fusion Hybridoma screening Hybridoma stabiliza on mab sequencing Chimeriza on Humaniza on Affinity matura on Epitope mapping Phage/yeast display FASEBA screening Characteriza on BioAnalysis Camelid single domain an body Stable cell line for assay development ADCC CDC Tumor cell line profiling HTRF assay development in vivo efficacy evalua on Tumour models Inflammatory disease models Pharmacokine cs Toxicity Biomarker Transient expression Stable expression Cell line development Process development Scale-up 9

11 DNA Immunization Services for Antibody Development DNA Immunization Technology is a powerful tool to aid in custom antibody production against membrane proteins, other problematic antigens, as well as early DNA vaccine development studies. This innovative technique allows antigen production in vivo, bypassing the need to produce and purify protein antigen in vitro. Whether your antigen is a membrane protein or other challenging antigen, such as a toxic, unstable or insoluble protein that is difficult to express or purify, our experienced team of scientists will utilize a fully customizable DNA immunization approach to meet your specific research goals. You provide the DNA sequence DNA Immunization Services Details GenScript DNA Immunization Step Specification Timeline Gene Synthesis & Validation Condon optimization Gene synthesis & plasmid preparation In vitro cell transfection for expression validation 2-3 weeks Protein antigen expression in vivo DNA Immunization Cell Fusion & Screening DNA immunization bia gold particle bombardment with gene gun Test bleed by ELISA Animal selected for fusion based on titer Primary screening by whole cell based ELISA Customer can ecaluate hybridomas supernatants and select the top clones for their application Additional screening options available 6-10 weeks 4-6 weeks Custom antibody production Subcloning, Expansion & cryopreservation Hybridomas are subcloned by limiting dilution according to the evalutaion result from the customer, then expanded & frozen Monoclonal Antibody Production (optional) Production of mabs for each cell line with roller bottle cultura Purification ELISA results Based on project Anti-Idiotype Antibody Production Anti-Idiotype Antibody Packages Anti-Idiotype Antibody Packages Services Anti-idiotype monoclonal antibody Anti-idiotype polyclonal antibody Starting material QC Deliverables Target antibody drug 2-3 mg Cross-reactivity with control IgG <10% Hybridoma cell lines, supernatants and purified anti-id antibody (optional) Target antibody drug 20 mg or more Cross-reactivity with control IgG <10% mg purified anti-id antibody/rabbit 10

12 GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ USA Toll-Free: Tel: Fax: SBP141026