Predict Reactivity Note Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%)

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1 Datasheet GeneTex, Inc : Toll Free GeneTex ( ) Fax: [email protected] GeneTex International Corporation : Tel: Fax: [email protected] Date : 2015/10/8 Catalog Number GTX Package:100 μl ( 5 ) ( 5 ) Product Name Full Name Product Description Background Host Clonality Clone Name Isotype Immunogen Antigen Species Species Reactivity Predicted Cross Reactivity species beta Actin antibody [GT5512] actin, beta Mouse Monoclonal antibody to beta Actin (actin, beta) This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] Mouse Monoclonal GT5512 IgG1 Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary. Human Human, Mouse, Drosophila, Rat, Zebrafish, Yeast, Goat, Rabbit Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine, Guinea pig Predict Reactivity Note Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%) Applications ICC/IF, WB Application Note Suggested dilution ICC/IF 1:100-1:1000* Western blot 1:1000-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher. Reference Positive Controls Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, NIH-3T3, mouse brain, PC-12, rat brain, drosophila, yeast, 30 hpf zebrafish, rabbit blood, goat blood, *L6, *Ishikawa, *U87-MG Predicted Target Size 42 Cellular Localization Conjugation Form Supplied Cytoplasm, cytoskeleton Unconjugated Liquid Purification Affinity purified by Protein G. Concentration Storage Buffer Storage Instruction Notes ResearchArea 1 mg/ml PBS Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Cell Biology > Cytoskeleton Signal Transduction > ECM/Cytoskeleton > Cytoskeleton Zebrafish > Validated Product

2 Application Reference 1. Zhong X (2015) Endocrinology Chang WC (2015) Food Res Int 3. Huang SY (2014) PLoS One e Noomhorm N (2014) J Pharmacol Sci Alberio T (2014) Biochim Biophys Acta A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg 293T whole cell lysate/extract D. 30 µg A431 whole cell lysate/extract E. 30 µg HeLa whole cell lysate/extract F. 30 µg HepG2 whole cell lysate/extract G. 30 µg H1299 whole cell lysate/extract H. 30 µg HCT116 whole cell lysate/extract I. 30 µg MCF-7 whole cell lysate/extract J. 30 µg NT2D1 whole cell lysate/extract K. 30 µg PC-3 whole cell lysate/extract L. 30 µg U87-MG whole cell lysate/extract Beta Actin antibody [GT5412] (GTX629630) dilution: 1:20000 A. 30 µg 293T whole cell lysate/extract

3 A. 30 µg 293T whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg mouse brain lysate/extract D. 30 µg PC-12 whole cell lysate/extract E. 30 µg rat brain lysate/extract Beta Actin antibody [GT5412] (GTX629630) dilution: 1:10000 A. 10 µg yeast lysate/extract beta Actin antibody [GT5412] (GTX629630) dilution: 1:1000 A. 30 µg drosophila lysate/extract Beta Actin antibody [GT5412] (GTX629630) dilution: 1:1000

4 A. 30 µg rabbit blood lysate/extract beta Actin antibody [GT5412] (GTX629630) dilution: 1:10000 A. 30 µg goat blood lysate/extract beta Actin antibody [GT5412] (GTX629630) dilution: 1:10000 GTX ICC/IF Image beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: beta Actin protein stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500. Blue: Hoechst staining.

5 A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract D. 1 µg 293T whole cell lysate/extract Beta Actin antibody [GT5412] (GTX629630) dilution: 1:10000 A. 30 µg 30 hpf zebrafish lysate/extract beta Actin antibody [GT5412] (GTX629630) dilution: 1:1000