KMS-Specialist & Customized Biosimilar Service 1. Polyclonal Antibody Development Service KMS offering a variety of Polyclonal Antibody Services to fit your research and production needs. we develop polyclonal antibodies to targets including parasites, cancer-specific surface antigens, hormones, growth factors and their receptors, glycoproteins, peptides, proteins and their modification sites (mutation site-specific, phosphorylation site-specific, cleavage site-specific, etc), and other antigens such as small-molecule compounds and DNA. We have created antibodies for various applications, such as functional assays, antibodies for immunoprecipitation, IHC, IF, flow cytometry, western blot, and EIA. 4-6 weeks We will deliver approximately 1 ml of mice serum or 5 ml of Rabbit serum with guaranteed titer at least 1:10,000 (Range 1:10,000 to 1:100,000). 2. Monoclonal Antibody Development Services KMS offer mouse hybridomas development technology for producing custom monoclonal antibodies from synthesized peptide or recombinant protein antigens. Novel immunization strategies, antigen design, and screening technologies are essential for the successful generation of monoclonal hybridoma cells lines. Our immunization protocols take advantage of using altered immunization routes, combinations of ligand carriers and dosing strategies to produce the most diverse monoclonal antibody population possible. Antigens must be carefully designed, especially when dealing with peptides, to generate a robust reagent that is able to recognize conformational changes of the native protein in assay conditions.
Highlights: Monoclonal antibodies - Robust protocol options for immunizing mice, ELISA-titer test bleeds, creating fusions, screening hybridomas, and delivering cell lines Customizable and adaptable - each protocol can be expanded or modified in a variety of ways to service specific needs for final scale, specificity or assay validation 3-4months We will deliver two positive Hynridoma clone and 5ml of culture supernatant. 3. Antibody and Protein Purification Services Services and Offerings KMS provide purification service antibodies and protein utilizing a wide array of protein purification methods; from research (mg) to scale-up (grams) quantities. We also offer low endotoxin production for in-vivo use. Our goal is to find or develop the antibody purification procedure which provides the best balance between yield, purity, and cost. Available Antibody Purification services and methods include: Ammonium Sulphate Precipitation This method is useful for concentration and partial purification of antibodies from most sources and all species. Although, on its own the yields of antibody are impure, it is the method of choice when combined with ion exchange for large volumes of anti-sera. It is not recommended for purification of tissue culture supernatant. Protein A This process binds the Fc region of major sub-classes of mouse and rat IgG to varying degrees. The results with protein A are usually high purity and high yields. It is useful for large-scale purifications of tissue culture supernatants.
Protein G Protein G can be used for purifying antibody from most sources. Like protein A, the results are usually of high purity and high yield. It is applicable to a wider range of immunoglobulins than protein A, but elution procedures can be much harsher. Ion Exchange Chromatography This method is used for all sources of antibodies. It is applicable to all lggisotypes. It is usually the method of choice when purifying antibodies from ascites, as the monoclonal can be resolved from the host immunoglobulins. Antigen Affinity This is the method of choice when specific antibody is required from polyclonal antisera. It requires pure antigen for the best results and can sometimes result in inactive antibody. Size Exclusion This method is appropriate for IgM antibodies from all sources. It is used when IgMAntibodies need to be separated from polyclonal anti-sera. It is not recommended as a single stage. 2-3weeks Purified antibody 4. Immunoassay Development Services KMS immunoassay development services are project-based and designed for diagnostics and drug discovery applications. Our scientists and project managers will discuss with each client the project design, cost estimates, and delivery specifications.
We offer three basic categories of services Immunoassay development service for diagnostic Kits. Development and Validation of ELISA of ELISA for pharmacokinetic assay of Bio similar protein, Antibody and peptide. Development and Validation of ELISA for Immunogenicity assay for Bio similar protein, antibody and peptide 3-4months Deliverables vary depending on the types of services requested and the requirements of the assays. In general, we deliver cell lines, antibodies and conjugates, assay protocols, assay reagents, assay kits, experiment records, and specific data derived from a given project. 5. Labeling of Antibody and Protein KMS offer conjugation service for antibody, protein conjugate and peptide with following Traditional fluorophores such as FITC and Texas Red dye Biotin HRP Alkaline phosphatase, and other enzymes KLH and BSA for peptide Sample requirements: Synthetic peptide or amino acid sequence of the peptide 2-3weeks Peptide carrier conjugate
6. Molecular and cell Biology Services KMS offer reliable and economical molecular biology outsourcing, including de novo gene synthesis, gene cloning, DNA construct, plasmid preparation, and site directed mutagenesis. The services cover the entire range of gene analysis from identification and cloning to functional studies. Clone gene in desired vector. 7. Cell-based Bioassay Services KMS offer service for developing the cell based assays for the determination of specific activity and biological characterization. We have expertise and technology for the following assay Cell Proliferation Assays Cell Based camp Release Assay Competitive Receptor Binding (Flow Cytometry) Reporter Gene Assay CDC Assay ADCC Assay Antiproliferation Assay Immunogenecity Assays Neutralizing Antibody Assays