1) Which of the following pairs is mismatched? A) Pasteur proof of biogenesis B) Ehrlich chemotherapy C) Jenner vaccination D) Koch aseptic surgery 2) Which of the following is a scientific name? A) Streptococcus pyogenes B) Flesh-eating bacteria C) IGAS D) Streptococci E) Group A streptococcus 3) Which of the following is NOT a domain in the three-domain system? A) Animalia B) Eukarya C) Archaea D) Bacteria 4) Which of the following statements about fungi is true? A) All are prokaryotic. B) All are multicellular. C) All are plants. D) All grow using sunlight and carbon dioxide. E) All require organic material for growth. 5) Which of the following statements about protozoa is false? A) All are eukaryotic. B) All have complex cells. C) They are classified by their method of locomotion. D) All are unicellular. E) They have rigid cell walls. 6) Which of the following statements about viruses is false? A) They have both DNA and RNA. B) They lack cells. C) They are parasites. D) They cannot metabolize nutrients. E) They cannot reproduce themselves. 1
7) Regarding the experiments that "proved" spontaneous generation, which of the following statements is probably true? A) Microorganisms were already present. B) Air was lacking. C) Too much heat was applied. D) The food source could not support life. E) Excess carbon dioxide was present. 8) Regarding Pasteur's experiments with the S-neck flask, which of the following statements is true? A) All microorganisms were killed before beginning. B) Any possibility of contamination was removed. C) There was air involved. D) There was a food source involved. E) All of the above. 9) Which of the following statements about insect control by microorganisms is true? A) This technique is just as dangerous as the use of chemical pesticides. B) The insects develop resistance to the microorganisms. C) The microorganisms are specific for the insect pest. D) The microorganisms are permanent in the environment. E) The microorganisms may cause disease in other animals. 10) Which of the following pairs is mismatched? A) Hooke cell theory B) van Leeuwenhoek germ theory C) Jenner vaccines D) Pasteur fermentation E) Lister aseptic surgery 11) Who disproved the theory of spontaneous generation? A) Hooke B) Pasteur C) Koch D) Virchow E) van Leeuwenhoek 12) Who observed cells in plant material? A) Koch B) Pasteur C) Lister D) Hooke E) van Leeuwenhoek 2
13) Who was the first to observe microorganisms with a microscope? A) van Leeuwenhoek B) Pasteur C) Koch D) Weizmann E) Hooke 14) Who proved that microorganisms cause disease? A) Semmelweis B) Koch C) Fleming D) Pasteur E) van Leeuwenhoek 15) Which of the following is a scientific name? A) Leprosy bacterium B) Hansen's bacillus C) Acid-fast bacteria D) Mycobacterium leprae 16) Classification of organisms in the three domains is based on A) Number of cells. B) Cell wall. C) Type of cell. D) Nutritional type. E) Nucleus. 17) Which of the following statements is false? A) All viruses are parasites. B) All fungi are multicellular. C) All fungi have nuclear membranes. D) All bacteria lack nuclear membranes. E) All protozoa are unicellular. 18) Which of the following statements is true? A) Protozoa have rigid cell walls. B) Fungi are plants. C) Bacteria cannot move. D) Viruses cannot reproduce outside a host cell. E) Algae are parasites. 3
19) Which of the following findings was essential for Jenner's vaccination process? A) Disease is caused by viruses. B) A weakened microorganism may produce immunity. C) A weakened microorganism will not cause disease. D) Someone who recovers from a disease will not acquire that disease again. E) Vaccination provides immunity. 20) Which of the following requirements was NOT necessary for Pasteur to disprove spontaneous generation? A) Identifying bacteria B) Providing a food source that would support growth C) Keeping microorganisms out D) Removing microorganisms that were initially present E) Supplying air 21) Which of the following pairs is mismatched? A) Virologist studies human immunodeficiency virus B) Immunologist studies ecology of Legionella pneumophila C) Molecular biologist studies recombinant DNA D) Microbial physiologist studies fermentation of sourdough bread E) Microbial ecologist studies bacteria that degrade oil 22) Which of the following pairs is mismatched? A) Penicillin antibiotic B) Chemotherapy treatment of disease C) Vaccine a preparation of microorganisms D) Normal microbiota harmful E) Pathogen disease causing 23) Which one of the following does NOT belong with the others? A) Eukarya B) Fungi C) Archaea D) Bacteria 24) Which of the following is NOT an example of biotechnology? A) Bacterial production of vinegar B) Bacterial production of French bread C) Bacterial production of yogurt D) Bacterial degradation of a dead animal E) Bacterial production of human insulin 4
25) Which of the following CANNOT be made by using recombinant DNA technology? A) Pharmaceutical drugs B) Vaccines C) Pest-resistant crops D) Life E) Human hormones 26) Which of the following is the best definition of biotechnology? A) The development of recombinant DNA technology B) Bacterial production of foods C) The curing of diseases D) The use of microorganisms in sewage treatment E) The use of living organisms to make desired products 27) You are observing a cell through a microscope and note that it has no apparent nucleus. You conclude that it most likely A) Is a plant cell. B) Has a peptidoglycan cell wall. C) Is part of a multicellular animal. D) Moves by pseudopods. E) Has a cellulose cell wall. 28) A nucleated, green cell that moves by means of flagella is a(n) A) Fungus. B) Alga. C) Helminth. D) Virus. E) Bacterium. 29) An agent that reproduces in cells but is NOT composed of cells and contains RNA as its genetic material is a(n) A) Bacterium. B) Virus. C) Fungus. D) Helminth. E) Alga. 30) A multicellular organism that has chitin cell walls and absorbs organic material is a(n) A) Fungus. B) Bacterium. C) Alga. D) Virus. E) Helminth. 31) A multicellular organism that has a mouth and lives in an animal host is a(n) A) Fungus. B) Virus. C) Helminth. D) Bacterium. E) Alga. 32) In the name Escherichia coli, coli is the A) Genus. B) Specific epithet. C) Kingdom. D) Discoverer. E) Domain. 5
33) Which of the following pairs is mismatched? A) Jacob and Monod microbial genetics B) Winogradsky microbial ecology C) Jenner immunology D) Weizmann virology E) Lancefield immunology 34) You are looking at a white cottony growth on a culture medium. Microscopic examination reveals it is multicellular. Which of the following conclusions about this organism is false? A) It has cell walls. B) It has DNA enclosed in a nucleus. C) It absorbs organic nutrients. D) It is eukaryotic. E) It is a bacterium. 35) Which of the following groups include members that lack DNA? A) Fungi B) Viruses C) Helminths D) Protozoa E) Bacteria 36) Which one of the following does NOT belong with the others? A) Cellulose B) Nucleus C) Chitin D) Peptidoglycan 37) Which of the following organisms lack cell walls? A) Plantae B) Animalia C) Bacteria D) Fungi 38) Which of the following does NOT belong with the others? A) Helminthes B) Animalia C) Protista D) Fungi E) Plantae 39) Fungi differ from bacteria in that fungi A) Have cell walls. B) Spoil food. C) Are cellular. D) Have DNA. E) Have a nucleus. 40) Archaea differ from bacteria in that archaea A) Use organic compounds for food. B) Reproduce by binary fission. C) Are prokaryotic. D) Lack peptidoglycan. E) Lack nuclei. 6
41) Bacteria differ from viruses in that bacteria A) Can live without a host. B) Have DNA and RNA. C) Are composed of cells. D) All of the above. 42) Which of the following lack a nucleus? A) Bacteria B) Fungi C) Protozoa D) Animalia E) None of the above 43) Regular use of antibacterial cleaning products A) Prevents bacteria from growing. B) Is necessary for good health. C) Prevents occurrence of infections. D) Promotes survival of bacteria that are resistant. E) A, B, and D 44) Which of the following statements about biofilms is false? A) Compared to free-living bacteria, biofilms are more sensitive to antibiotics. B) Biofilms on rocks provide food for animals. C) Biofilms on medical implants cause infections. D) Biofilms in pipes block the flow of water. E) Biofilms in your body protect mucous membranes from harmful microbes. 45) Which of the following processes in making blue jeans is incorrectly matched? A) Cotton fungal hyphae B) Blue dye E. coli indigo C) Debleaching bacterial enzyme D) Plastic bacterial PHA E) Stone-washed fungal enzyme 46) In 1835 Bassi showed that a fungus caused a silkworm disease, and in 1865 Pasteur discovered that a protozoan caused another silkworm disease. Why do we use Koch's postulates instead of "Bassi's" or "Pasteur's" postulates? Answer: 47) List two examples of biotechnology that involve recombinant DNA technology and two examples that do not. Answer: 48) Paul Berg received the Nobel Prize for developing the procedure for incorporating fragments of animal DNA into bacteria. List some reasons why his work was a major contribution to science. Answer: 7