impact II Get the full picture the first time Innovation with Integrity UHR-TOF MS
Sensational Capabilities of impact II Optimize your LC-MS methods without compromising performance: impact II delivers the full range of specified performance parameters simultaneously to solve your analytical challenges. The Ultra-High Resolution (UHR) QTOF technology pioneered by Bruker once again defines the standards of what can be achieved using accurate mass LC-MS/MS. New innovations in time-of-flight instrumentation are now available in a robust and market-leading benchtop system. Your Success with impact II Outstanding Hardware Performance Enhanced dynamic range 50 Gbit/sec data sampling technology 10 bit ADC technology New TOF with improved resolving power Instant Expertise Software IDAS Intensity Dependent Acquisition Speed RT 2 RealTime Re-Think SmartFormula 3D Market Leading Sensitivity Dual ion funnel IonBooster Source CaptiveSpray nanobooster New collision cell with broad mass transfer Deepest insight into your sample Easy-to-use Robust & Quantitative
Robustness And Simplicity All Day, Every Day Dynamic range five orders of magnitude 50 GBit/sec sampling technology enables high Dynamic range acquisition on an LC timescale Greatly increased robustness to sample variation allowing reduced sample pretreatment especially desirable in high throughput quantitative applications. Definitive trace analysis from complex, high-background matrices makes your analytical work more productive. Increased dynamic range, excellent robustness, and full sensitivity gives you the deepest insight into your sample and what might be hiding underneath. Sensitivity One shot plug & play acquisition with market leading standard sensitivity Ensuring qualitative and quantitative results in one LC run delivers fastest possible time-to-success. Whether running with standard ESI, ionbooster or even nano-flow separation coupled with the patented CaptiveSpray nano- Booster the impact II with its dual ion funnel delivers extreme sensitivity for best qualitative and quantitative results in one run. Instant Expertise Intelligent self-optimizing MS/MS routines deliver expert-caliber results first time from your complex biomarker discovery or small molecule unknown screening experiments where spectral fidelity is key. This also includes de novo molecular formula determination. Let the impact II achieve your goals in: Biomarker discovery and validation in proteomics and metabolomics Drug metabolite, degradant and impurity identification and quantitation Synthetic chemistry support Intact protein analysis and characterization of biopharmaceuticals Forensic and doping control Food and water testing And all in an economical and benchtop design. Full sensitivity resolution Having to choose between resolution and sensitivity on other instruments restricts the depth to which you can understand your sample. Instead of beam shaping, the impact II features a new time-of-flight tube with improved resolving power for optimized detection.
More Dynamic Range, More Accuracy, More Confidence Versatility Sensitive mass transfer from smallest fragment ions to monoclonal antibodies Intens. 2500 39.0229 Deconvoluted mass: 148 kda 2000 1500 1000 500 0 41.0379 38 40 42 44 46 m/z Low mass fragment ion of vanillic acid precursor at 0.1mDa Mass accuracy 2200 2400 2600 2800 3000 3200 3400 3600 Mass Spectrum obtained from intact Adalimumab which has a molecule weight of 148k Da Sensitivity Patented dual Ion-Funnel: Ruggedness and sensitivity Choice of rugged and sensitive ion sources The robustness, sensitivity and spectral accuracy of the impact QTOF-MS has accelerated the process to identify unknown compounds. This is an integral part of long-term goal to sequence the Medicago truncatula metabolome Professor Lloyd W. Sumner, Analytical Biochemistry Plant Biology Division, The Samuel Roberts Noble Foundation Ardmore, OK, USA
TOF with improved Full Sensitivity Resolution (FSR) Spectral accuracy 3800 m/z Robustness Market-leading highdynamic range 10 bit detection system New broad mass transfer Quadrupole CID cell with DC gradient Intens. x10 7 Much higher dynamic range in concentration reachable 195.087608 +MS, 1.0min #55 Intens. +MS, 1.0min #55 0.8 50 61.039693 0.6 0.4 40 30 20 10 0 Urea 0.8 ppm I = 52 counts 60.0 60.5 61.0 61.5 62.0 62.5 m/z 1.7 x 10 5 Caffeine 0.6ppm I = 9,230,400 counts 0.2 0.0 219.106753 60 80 100 120 140 160 180 200 220 m/z In-spectrum dynamic range @ real LC speed don t miss low abundant peaks.
Productivity and. Robust profiling of large batches of complex biological samples ppm mass accuracy isotopic pattern gma = 4.4) 97 168 169 m/z 166.0863-0.2 ppm mass accuracy True isotopic pattern (msigma = 4.4) 167.0897 166 167 168 169 m/z 100 injections of human Urine sample msigma msigma msigma msigma 45,0 30,0 45,0 15,0 30,0 0,0 15,0 0 20 40 60 80 100 15,0 0,0 0 20 40 60 80 100 msigma Injection No. 30,0 msigma Value for phenylalanine Injection No. in 100 urine samples measured msigma < 15 = unambiguous molecular formula generation even for 45,0 higher m/z values msigma 0,0 0 20 40 60 80 100 Injection No. Peak shapes in urine sample remain constant: Instrument robustness enables comprehensive metabolic profiling studies of large sample sets. Looking at a selected compound: Phenylalanine. SmartFormula provides the correct molecular formula based on accurate mass and isotopic pattern fit: C 9 H 12 N 1 O 2. Fully reproducible isotopic fidelity for phenylalanine across 100 samples injected unambiguous molecular formula generation also for higher m/z values all day, every day. Simplicity: Fast and accurate acquisition of fragment spectra m/z 800 600 MS/MS Spectrum acquired Several thousand high quality MS/MS spectra ready for spectral library search 400 200 2 4 6 8 10 Time [min] impact II enables up to 50 Hz instrument scan speed combined with Instant Expertise TM software: All precursor ions fragmented in one shot acquisition of human urine sample spiked with vanillic acid. The low abundant target mass 169.0495 m/z of the vanillic acid precursor was fragmented successfully. Query in custom human metabolite MS/MS Library, provides correct identification. The library was generated in a different laboratory on a different impact QTOF instrument.
Versatility for OMICS research Intens. Intact Protein Profiling The combination of Isotopic fidelity and mass accuracy that makes the impact II capable of delivering molecular formulae for metabolites is preserved over the mass range. Consequently, even proteins out of a complex mixture (here : overlapping compounds resolved with the Dissect algorithm in an E. coli extract) can be extracted and measured with an unrivalled mass accuracy and an exact isotopic pattern. Accurate Bottom-up proteomics quantitation The robustness of the impact II with its impressive dynamic range enables accurate quantitation. As an example the CV s obtained for a selected subset of 10 proteins out of 55 consecutive injections of an Hela Cell digest, separated with a 90 min gradient and acquired with the Instant Expertise ID/Quant method: that illustrates the capabilities of the instrument for label-free discovery approaches. +MS, 5.34-5.52min, Deconvoluted (MaxEnt, 512.09-1549.29, *0.0482143, 35000) x10 5 ppm/theory (injection 3) % abundancy/theory (injection3) 1.25-0,02 0,01 0,0-0,02 1,9 10298.6903-1,2 1.00-0,06 10297.6881 10299.6927-0,5-0,0 10296.6848 Intens. -0,8 +MS, 5.34-5.52min, Deconvoluted (MaxEnt, 512.09-1549.29, *0.0482143, 35000) x10 10300.6953 0.75 0,2 5 ppm/theory (injection 3) -2,2-0,2 % abundancy/theory (injection3) -0,5 10295.6845 1.25 10301.6964-0,1-0,02 0.50 0,2 0,01 0,0-0,02 0,1 1,9 10298.6903-0,6-1,2 1.00-0,06 10297.6881 10302.6996-0,2 10299.6927 10294.6825-0,5-0,0 0.25 0,3 0,5 0,3 10296.6848-0,8 10303.7001 0,6 --0,1 0,1 10300.6953 0.75 0,2 10304.70770,0 10293.6804-2,2 0,3-0,2 10305.7083 10295.6845-0,1-0,5 0.00 10301.6964 10292 10294 10296 10298 0.50 10300 10302 10304 10306 10308-0,1 0,2 10310 m/z 0,1-0,6 10302.6996-0,2 10294.6825 0.25 0,3 0,5 0,3 10303.7001 0,6 --0,1 0,1 88 10304.70770,0 10293.6804 0,3 10305.7083-0,1 0.00 10292 10294 10296 10298 10300 10302 10304 10306 10308 10310 m/z 69 Mr of monoisotopic peak in 4 consecutive injections: 1 : 0.08 ppm 2 : -0.16 ppm 3 : 0.04 ppm 4 : 0.32 ppm 88 24 69 53 63 24 84 53 40 57 66 62 28 63 29 14 33 86 77 80 34 84 51 91 72 8587 40 57 66 45 61 38 81 62 15 67 119 28 76 112 29 12 52 107 14 33 86 890 77 80 34 60 71 79 21 8251 44 75 93 16 104 91 49 83 105 85111 47 78 72 23 87 45 61 38 81 10 98 67 55 121 10103 76 20 35 118 15 119 97 4243 94 50 112 39 68 70 96 92 109 110 19 27 54 12 52 107 36 65 32 73 74 4 102 22 4816 60 71 79 21 82 890 18 93117120 26 37 113 114 44 75 23 58 59 2 99 100 104105 49 113 17 25 95 115 116 31 30 41 56 3 1083 47 111 67 89 5 64 1 10 46 78106 98 55 121 10103 20 35 118 50 97 39 68 70 4243 94 96 92 109 110 19 27 54 26 36 65 4 102 32 73 74 18 117120 22 48 25 37 113 114 115 2 113 17 2 4 36 8 1041 58 59 12 14 Time 95 [min] 99 100 108 116 30 31 56 67 89 5 64 1 46 106 14 2 4 6 8 10 12 Time [min] Intact protein profiling: > 1000 proteoforms detected in 15 min Peak area x 10000000 Peak area % CV 10 5 Read more in Application Notes LCMS-81: Introducing New Proteomics Acquisiton Strategies with the compact Towards the Universal Proteomics Acquisition Method Actin, cytoplasmic 2 AVFPSIVGRPR Tubulin beta chain, ISVYYNEATGGK Carbamoyl-phosphate synthase, IALGIPLPEIK Stress-70 protein, TTPSVVAFTADGER 0 Ubiquitin-like modifier-activating enzyme 1, KPLLESGTLGTK LCMS-89: High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Glycomics and Glycoproteomics An illustration of versatility. The impact II accurate mass and data for glycans and glycopeptide can be fully exploited in Protein Scape for screening, identification and characterization operations.
Qualitative and Quantitative Drug Metabolism Characterization Metabolite Detection Metabolite Detect software compares the data file for the drug (in this case t 60 ) with the corresponding control sample. Metabolite ID Auto MS/MS Spectrum Intens. x10 5!'($%)!% *+,"-#(.$!%'#/01"2$&3401"$"5"$"67819.&%5(%" Intens. x10 5 1.5!"#$%"#& 354 4 376 1.0 3 354 0.5 2 392 0.0 100 150 200 250 300 350 m/z 1 212 0 0.5 1.0 1.5 2.0 2.5 3.0 Time [min] SmartFormula3D A base peak chromatogram of the difference is created allowing metabolites m/z 354, 212 and even 392 to be easily observed. Drug and Metabolite Profiles Integration is carried out on the EIC for the measured m/z of each metabolite +/- 0.005 Da. Plotting the ratio of metabolite to internal standard (MIS) vs. time produces the metabolite profiles. Half-life and clearance values are determined from the natural log (ln) of the drug profile vs time plot. Formula and Structure C 21 H 18 NOFCl O N + Cl F
Your Partner in Biopharmaceutical Analysis A powerful benchtop solution with comprehensive capabilities for biological therapeutics screening, characterization and confirmation Intact Mass Measurement of the mab Adalimumab Rapidly and routinely acquire monoisotopic antibody subunit data, with resolved isotopes and superb mass accuracy. This yields greater confidence in sequence confirmation and easy detection of heterogeneities. Acquire high coverage peptide maps and confidently detect and identify low-level sequence variants with increased MS/MS acquisition speed and wider dynamic range. Effective screening and quantitation of released glycans and small molecule impurities due to broadband transmission and wide dynamic range. Mr / Mr = 3 ppm 147400 147600 147800 148000 148200 148400 148600 148800 149000 m/z Deconvoluted Spectrum Mr (expected) = 148080.1 2200 2400 2600 2800 3000 3200 3400 3600 3800 m/z Spectrum of intact monoclonal antibody Adalimumab Subunit analysis Mr (mono) = 24093.0826 Mr (mono) Da = 24093.0826 Da Theoretical isotopes for Fc/2 Theoretical isotopes for Fc/2 Mr (mono) = 24093.0826 Da Theoretical isotopes for Fc/2 M/M = -0.48 ppm M/M = -0.48 ppm M/M = -0.48 ppm 24090 24100 24110 24120 24130 m/z 24090 24100 24110 24120 24130 m/z Peptide Maps Annotated BPC Multiple enzymes Library matching Subunit analysis Comparison with reference material 24090 24100 24110 24120 24130 Mr(mono) = 23397.5899 Da m/z Mr(mono) = 23397.5899 Theoretical Da isotopes for LC Theoretical isotopes for LC Mr(mono) = 23397.5899 Da Theoretical isotopes for LC M/M = -0.80 ppm M/M = -0.80 ppm Intact Mass Analysis M/M = -0.80 ppm Comparison with reference material 23400 23410 23420 23430 23440 m/z Glycoform ratio calculation 23400 m/z Mr (mono) = 25442.5176 Da 23400 23410 23420 23430 23440 m/z Theoretical isotopes for Fd Mr (mono) = 25442.5176 Da Mr (mono) = 25442.5176 Theoretical Da isotopes for Fd Theoretical isotopes for Fd BioPharma Compass Screening impurities released glycans M/M = -0.19 ppm M/M = -0.19 ppm M/M = -0.19 ppm 25450 25460 25470 25480 25490 m/z 25450 25460 25470 25480 25490 m/z 25450 25460 25470 25480 25490 m/z
One-Shot Full Picture of the Sample... Full Sensitivity Resolution and high dynamic range are prerequisites to reach extreme low level of detection. High-resolution Extracted ion Chromatogram identifying pesticides with full scan MS and bbcid acquisition in single LC-TOF run. All analytes across the mass range from 70 to 900 m/z are detected. The new HDC collision cell with un-compromised mass-transfer and sensitivity allows for trace level residue screening, confirmation and quantitation. Pesticide screening at 10 ng/l in surface waters 10 ppt Direct injection of 100 μl water: All analytes from 70 to 900 m/z are detected at 10 ng/l Highest Confidence in Confirmation of Elemental Composition of Pesticides 1 st Isotope +MS 50 ppt Dimethoate C 5 H 12 N 1 O 3 P 1 S 2 m/z 228.9996 err 0.55 mda 2 nd Isotope 3 rd Isotope Excellent Isotopic fidelity at very low concentration level
... for Rapid Profiling and Screening of Unknowns Comprehensive forensic drug screening with maximum confidence in the results with impact II. Accurate mass measurement of diagnostic ions, including the pseudo-molecular ion, Amphetamine adducts, isotopes as well as broad band CID (bbcid) fragment ions reduces or eliminates false positive findings, even in Amphetamine (^13C) complex matrices such as serum. At trace levels, buried in the grass at RT=4.3 minutes, diagnostic fragment ions m/z 119 and m/z Amphetamine 91 and Fragm the 119 13 C isotope for amphetamine are observed. Amphetamine is truly detected as positive finding from the Amphetamine screening Fragm 91result. To facilitate comprehensive screening for hundreds of target compounds, the 3.8 4.0 4.2 4.4 4.6 Time [min] Bruker ToxScreener and Pesticide Screener solutions are supported by high quality accurate mass databases, enabling users to readily process the data and obtain accurate, reliable screening results. Amphetamine Amphetamine (^13C) COTININE Caffeine O-DESMETHYLVENLAFAXINE V Example of Positive Amphetamine Finding at Trace Level Amphetamine Fragm 119 Amphetamine Fragm 91 3.8 4.0 4.2 4.4 4.6 Time [min] ECGONINE METHYL ESTER ECGONINE METHYL ESTER ( 13 C) COTININE Caffeine O-DESMETHYLVENLAFAXINE VENLAFAXINE METHADONE EDDP (METHADONE METABOLITE) METHADONE O-DESMETHYLVENLAFAXINE ( 13 C) EDDP (ME Caffeine ( 13 C) COTININE ( 13 C) O-DESMETHYLVENLAFAXINE ECGONINE ( 13 C) METHYL ESTER ECGONINE METHYL ESTER ( 13 C) 1 2 3 4 5 EDDP (METHADONE METABOLITE) ( 13 C) Caffeine ( 13 C) COTININE ( 13 C) Amphetamine 1 2 3 4 5 6 7 8 Time [min] EDDP We use the Bruker impact QTOF with bbcid to attain enhanced sensitivity for drugs-of-abuse and designer drug screening in a forensic setting, covering critical low concentration compounds like THC acid, buprenorphine, LSD, and synthetic cannabinoids. The bbcid workflow has been carefully validated against spectral library comparison, and it has proven to deliver equal confidence, but with a much higher efficiency, significantly increasing our productivity. Our forensic screening method has now been accredited by the Finnish Accreditation Service Dr. Anna Pelander, Laboratory of Forensic Toxicology at the University of Helsinki High resolution, accurate mass MS and bbcid MS/MS data acquisition enables rapid reaction to emerging challenges in food safety and doping even before reference standards are available Facilitates retrospective in-silico analysis for post-hoc identification of true unknown contaminants Accurate mass screening databases for Food Testing and Forensic Toxicology Food/ Water/Forensic sample UHPLC impact II One shot qual /quant analysis with no method development MS, Total Ion CID, MSMS Processing with high quality database In silico retrospective analysis of new targets Identification, confirmation, quantitation, ID of new compounds and reporting
Bruker Daltonics is continually improving its products and reserves the right to change specifications without notice. BDAL 06-2014, 1829433 Dynamic Source Configuration In addition to ESI sources, Bruker life science MS systems support a wide range of source options from Bruker and third-party vendors, all switchable within seconds. CaptiveSpray nanobooster CaptiveSpray nanobooster is the proteomics ion source that brings your MS to the next performance level The operation is as easy as electrospray can be. The nanobooster enables Glycoanalysis, supercharging and pushes up ID rates. ionbooster The ionbooster offers a 5 100x gain in sensitivity for many compounds of interest in the fields of environmental analysis, food testing and therapeutic drug monitoring. APCI Atmospheric Pressure Chemical Ionization is used in metabolomics as well as for drug or pesticide screening for less polar molecules where ESI fails to deliver reasonable quantities of ions. APPI Atmospheric Pressure Photo Ionization is used for less polar or non-polar molecules that can not be ionized in either ESI or APCI. DIP The DirectProbe add-on for the Bruker APCI II and APPI II ion sources allows direct analysis of liquid and solid samples without tedious sample preparation. GC-APCI II The GC-APCI II source with unique flexible, heated transfer line and calibrant delivery enables GC coupling to any Bruker TOF or QTOF, trap or FTMS system originally designed for LC coupling. GC-APCI II APPI APCI with DIP CaptiveSpray nanobooster We have been using the impact since almost a year for routine shotgun bottom up proteomics. In association with the CaptiveSpray nanobooster, the instrument has provided an excellent level of sustainable performances, being capable of delivering untouched performances for over 6-8 weeks of 24/7 use. This is of tremendous importance for the success of our label-free measurement campaigns Prof. Alain Van Dorsselaer, LSMBO, Strasbourg For research use only. Not for use in diagnostic procedures. Bruker Daltonik GmbH Bremen Germany Phone +49 (0)421-2205-0 Fax +49 (0)421-2205-103 sales@bdal.de Bruker Daltonics Inc. Billerica, MA USA Fremont, CA USA Phone +1 (978) 663-3660 Phone +1 (510) 683-4300 Fax +1 (978) 667-5993 Fax +1 (510) 687-1217 ms-sales@bdal.com cam-sales@bruker.com www.bruker.com