Aviva Custom Antibody Services and Prices Rabbit Polyclonal Antibody Service Package Number Description Package Contents Time Price Polyclonal package 1 (From protein to antiserum) Polyclonal package 2 (From protein to antigen purified ) Polyclonal package 3 (From peptide design to antiserum) Polyclonal package 4 (From peptide design to antigen purified antibodies) Customer provides antigen protein Protein will be checked by SDS-PAGE Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection We guarantee the ELISA titer of 1:32,000 or better. Customer provides antigen protein Protein will be checked by SDS-PAGE Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification Dot blot confirmation We guarantee the ELISA titer of 1:32,000 or better, and dot blot is positive at dilution Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection We guarantee the ELISA titer of 1:20,000 or better. Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification Dot blot confirmation We guarantee the ELISA titer of 1:20,000 or better, 10-12 10-12 $805 $1,050 $1,176 $1,421 Tel: 858-552-6979 Fax: 858-552-6975 www.avivasysbio.com 1
Polyclonal package 5 (From plasmid to antiserum) Polyclonal package 6 (From plasmid to antigen purified ) Polyclonal package 7 (From cdna to antiserum) Polyclonal package 8 (From cdna to antigen purified ) Polyclonal package 9 (Phospho-specific polyclonal ) and dot blot is positive at dilution Customer provides expression vector Express and purify the antigen protein Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection We guarantee the ELISA titer of 1:32,000 or better. Customer provides expression vector Express and purify the antigen protein Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification Dot blot confirmation We guarantee the ELISA titer of 1:32,000 or better, and dot blot is positive at dilution Customer provides protein information We clone the gene and construct the expression vector for fusion protein Express and purify the antigen protein Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection We guarantee the ELISA titer of 1:32,000 or better. Customer provides gene and protein information We clone the gene and construct the expression vector for fusion protein Express and purify the antigen protein Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification Dot blot confirmation We guarantee the ELISA titer of 1:32,000 or better, and dot blot is positive at dilution Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA 1mg antigen protein 1mg antigen protein Recombinant vector 1mg protein Recombinant vector 1mg protein (non- & phosphor 2-5mg against unphospho-peptide 2-5mg phosphospecific 14-16 14-16 $1,484 $1,729 $2,345 $2,590 Tel: 858-552-6979 Fax: 858-552-6975 www.avivasysbio.com 2
Polyclonal package 10 (Lys mono-methylated specific polyclonal ) Polyclonal package 11 (Lys dimethylated specific polyclonal ) Polyclonal package 12 (Lys trimethylated specific polyclonal ) Post-bleed collection Antigen affinity purification dot blot is positive at dilution 1:20,000, phospho-peptide 100 ng, but negative at dilution 1:100, unphospho-peptide 100ng. Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification dot blot is positive at dilution 1:20,000, Lys(Me) peptide 100 ng, but negative at Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification dot blot is positive at dilution 1:20,000, Lys(Me2) peptide 100 ng, but negative at Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection (non- & monomethylated 2-5mg against unmethylated peptide 2-5mg Lys(Me) specific (non- & di-methylated 2-5mg against unmethylated peptide 2-5mg Lys(Me2) specific (non- & tri-methylated 2-5mg against unmethylated peptide 2-5mg Lys(Me3) specific Tel: 858-552-6979 Fax: 858-552-6975 www.avivasysbio.com 3
Polyclonal package13 (Arg methylation-specific polyclonal ) Polyclonal package14 (Arg di-methylation (Symmetry)-specific polyclonal ) Polyclonal package15 (Arg di-methylation (Asymmetric)-specific polyclonal ) Antigen affinity purification dot blot is positive at dilution 1:20,000, Lys(Me3) peptide 100 ng, but negative at Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification dot blot is positive at dilution 1:20,000, Arg(Me) peptide 100 ng, but negative at Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification dot blot is positive at dilution 1:20,000, Arg(Me2) peptide 100 ng, but negative at Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification (non- & methylated 2-5mg against unmethylated peptide 2-5mg Arg(Me) specific dot (non- & dimethylated 2-5mg against unmethylated peptide 2-5mg Arg(Me2) specific dot (non- & dimethylated 2-5mg against unmethylated peptide 2-5mg Arg(Me2) specific dot Tel: 858-552-6979 Fax: 858-552-6975 www.avivasysbio.com 4
Polyclonal package 16 (Western guaranteed polyclonal ) Polyclonal package17 (Western and IHC guaranteed polyclonal ) dot blot is positive at dilution 1:20,000, Arg(Me2) peptide 100 ng, but negative at Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification Western blot confirmation We guarantee the ELISA titer of 1:20,000 or better, and the is suitable for Western blot. Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification WB and IHC confirmation We guarantee the ELISA titer of 1:20,000 or better, and the is suitable for Western blot and IHC. Western including ELISA, Western blot and IHC data 15-18 15-18 $3,024 $3,332 Tel: 858-552-6979 Fax: 858-552-6975 www.avivasysbio.com 5
Miscellaneous Services Peptide service Peptide design (suggestion) 1-2 days Free Peptide synthesis 2 Inquire Peptide conjugation (KLH/BSA/OVA, 5mg) $245 Protein service Gene synthesis 3-4 $2.52/aa Gene amplification (Template provided by customer, 1000bp) 2-3 Vector construction 2-3 $616 recombinant selection protein expression and purification 2-3 $616 Polyclonal service Protein checked by SDS-PAGE Pre-immune serum collection Immunization & serum collection (2 rabbits) Immunization & serum collection (2 rabbits, Fast) ELISA identification Prescreen (ELISA) Prescreen (Western Blot) Prescreen (Dot Blot) Additional rabbit (1 rabbit) Animal maintenance (1 rabbit) Additional bleed (10ml, 1 rabbit) Extension boost and bleed (10ml, 1 rabbit) Immunization & serum collection (1 goat) Immunization & serum collection (2 rats) Immunization & serum collection (4 mice) Immunization & serum collection (2 guinea pigs) 1 day 10 6-8 6-10 1week 20 10 10 11 $91 $14 $805 $924 $154 $47 $1,575 $700 $805 $910 Tel: 858-552-6979 Fax: 858-552-6975 www.avivasysbio.com 6
Antibody purification service Saturated ammonium sulfate precipitation ( 60ml) 3-5days $126 Protein A/G affinity purification ( 60ml) 3-5 days $182 Antigen affinity column preparation $245 Antigen affinity purification ( 60ml) 3-5 days Antibody identification service ELISA identification (1-10 samples) Cell extraction (1 sample) Tissue extraction (1 sample) Protein concentration assay (1 sample) $14 Western Blot identification (1-8 samples) $245 Dot Blot identification (1-10 samples) $62 IHC identification (section provided by customer,1 photo) Antibody labeling service HRP labeling ( 5mg) 1-2 Biotin labeling ( 5mg) 1-2 $560 Tel: 858-552-6979 Fax: 858-552-6975 www.avivasysbio.com 7