Why Being Small? Savings in time & cost. Disposable Parallel processing Integration/Automation. Gain from the unique microscopic features
|
|
- Esmond Carson
- 8 years ago
- Views:
Transcription
1 Miniaturization in Electronic Technology ENIAC: the "Electronic Numerical Integrator and Calculator, 1943 ENIAC filled a 20 by 40 feet room, weighed 30 tons, and used more than 18,000 vacuum tubes. iphone 6 A8 Chip, 2014 >10 9 transistor
2 Savings in time & cost Less materials and samples Short processing time Disposable Parallel processing Integration/Automation Gain from the unique microscopic features Laminar Flow Why Being Small? High surface to volume ratio High single-to-noise ratio in transuding signals Small thermal mass Strong fields such as electric fields 2
3 Why Being Small? Length Scale Matching Manipulation of molecules and cells High resolution / sensitivity (Kim) e.g. to facilitate single-molecule diagnostics, study of single-cell biology (Wang) >100 m 1 m 10-2 m 10-6 m 10-9 m Spacecraft Human Organ Tissue Cell DNA Micro/Nano Technology 3
4 Classes of BioMEMS (Bio-MicroElectroMechanicalSystem) Microfluidics & Microfluidic Devices Biosensors and Bioelectronics Neural Interface Devices Chromatography /Electrophoresis Devices Microsurgical Tools Bioreactors Tissue Engineering Devices Molecule /Cell Handling Devices Implantable Devices, Drug Delivery Devices 4
5 Examples of MEMS Devices Micro-mirrors Spider mite on gears (Sandia) 5
6 Examples of Industrial MEMS Devices Air Bag Sensor Bubble Inkjet Projector (Analog Device) (HP) Motion & Orientation sensor (Wii) (Texas Instruments) (Nintendo) 6
7 Apple iphone 6 6-axis Gyro/Accel (Invensense) 3-axis Accelerometer(Bosch) 3-axis Magnetometer (AKM) 3 microphones (Knowles) Pressure Sensors (Bosch) 7
8 MEMS Is Everywhere in Your Daily Life Internet of Things (IoT) Trillions of MEMS sensors coming soon! 8
9 Capillary Electrophoresis 9
10 Micromachined Capillary Electrophoresis (m-ce) 10 High throughput Low volume Rapid analysis Integrated with thermal cycling and CE for Sanger sequencing Off-chip optical detection (Ra Mathies, PNAS 2006)
11 11 Thermal Cycling for Polymerase Chain Reaction (PCR) PCR is an expensive and time-consuming technique
12 12 Continuous-flow Micro PCR Integration of CE and m-pcr (A. Manz, Science 2002) (M.A. Burns U Mich, Science PCR reaction Gel electrophoresis Microfluidics On-line electrical detector
13 Microfluidic Digital PCR : Nanoliter-sized PCR arrays 1176 chamber 6.25 nl each chamber (J.R. Leadbetter, Science 2006) 13
14 Droplet Digital PCR: Picoliter-sized PCR arrays Photon Counts per 100usec Photon Counts per 100usec 2000 Calcein Channel ROX channel Time (sec) 1500 Calcein Channel ROX channel Time (sec) (T. Rane, Lab Chip, 2015) 14
15 Analysis of Single Cells 15 Preparation of a single cell Single-Molecule Detection (R. Zare, Scinece 2006)
16 Laminar Flow-Based Assay 16 T-Sensor Separation (P. Yager) Laminar flow initiate reaction Diffusion-based analysis Rapid Mixing
17 Flied Flow Fraction-DEP cell sorter 17 Field flow fraction using DEP force ( U. Texas, Houston)
18 Free Solution Hydrodynamic Separation
19 Nano Fluidics nm-wide nanochannel array Stretch of l DNA (48.6 kbp)fragment DNA is driven by E-field (R. Austin)
20 20 Digital Microfluidics Pump-free and valve-free Each sample and reagent is individually addressable Array-based analysis
21 Integrated DNA Preparation and PCR Detection Centralized & manual tube based PCR detection Sample In Answer Out Cell lysis DNA purification & concentration PCR reaction DNA amplification (thermal cycling) Lysis/Binding buffer Washing buffer 1 Sample 2 Sample 1 Using Silica Superparamagnetic Particles (SSP) as a solid phase within droplets
22 Surface elevation SSP plug Surface topology assisted SSP and droplet manipulation Drops in Air a) a) Drops in Oil b) SSP plug Surface elevation BioSensing & BioMEMS 530/ Jeff Wang (Y Zhang, Johns Lab Hopkins Chip 2011) University
23 Fluorescence Intensity (AU) Fluorescence Intensity (AU) Fluorescence Intensity (AU) On-Chip Real-Time PCR Detection Normalized Fluorescence Intensity Normalized Fluorescence Intensity -df/dt -df/dt Detection of E coli 16S gene from cell culture bp 250bp 200bp 150bp 100bp Marker Amplicon Cycle (n) a) b) a) b) Detection of Rsf-1 marker from whole blood Fluorescence Fluorescence 0.10 Negative first derivative Negative first derivative BioSensing & BioMEMS 530/ Cycle (n) Temperature Jeff Wang (Celsius) Johns Hopkins University Cycle (n) Temperature (Celsius)
24 Microfluidic Droplet Technology for High-Throughput Analysis Features Monodisperse droplets of sizes ranging from nl-pl High speed droplet generation of > khz Potential applications Low-cost & High throughput screening Biochemical synthesis Digital PCR Single-cell analysis 24
25 Droplet Microfluidics for Monitoring of Kinetics
26 DNA Microarrays 26 Affymetrix Fabricated with lithographic technique cdna array Gene expression profiling Relative fluorescence measurement
27 Neural Probe/ Neuro Implant 27 Neuro-circuit interaction neuro-recoding Prosthesis research Chemical delivery Issues with long term implant bio compatibility
28 28 Tissue Engineering / Cell Patterning Patterning cells using microfluidics Control of microenvironments using microfluidics Single-cell (controlled small number of cells) patterning High-throughput search for right cell conditions for controlling cell growth, differentiation, apoptosis) ( Whitesides)
29 Corneal Microtissue Culture ( C. Puleo, Lab Chip. 2009) 29
Introduction To Real Time Quantitative PCR (qpcr)
Introduction To Real Time Quantitative PCR (qpcr) SABiosciences, A QIAGEN Company www.sabiosciences.com The Seminar Topics The advantages of qpcr versus conventional PCR Work flow & applications Factors
More informationIntroduction to Microfluidics. Date: 2013/04/26. Dr. Yi-Chung Tung. Outline
Introduction to Microfluidics Date: 2013/04/26 Dr. Yi-Chung Tung Outline Introduction to Microfluidics Basic Fluid Mechanics Concepts Equivalent Fluidic Circuit Model Conclusion What is Microfluidics Microfluidics
More informationUndergraduate Biomedical Engineering Curriculum University of North Carolina at Chapel Hill, 2014-2015
Undergraduate Biomedical Engineering Curriculum University of North Carolina at Chapel Hill, 2014-2015 First two years Fall: BMME 101 Frontiers of Biomedical Engineering (strongly recommended but not required;
More informationNext Generation Sequencing
Next Generation Sequencing Technology and applications 10/1/2015 Jeroen Van Houdt - Genomics Core - KU Leuven - UZ Leuven 1 Landmarks in DNA sequencing 1953 Discovery of DNA double helix structure 1977
More informationReal-Time PCR Vs. Traditional PCR
Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives
More informationTaqMan Fast Advanced Master Mix. Protocol
TaqMan Fast Advanced Master Mix Protocol For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice. APPLIED
More informationGenomics Services @ GENterprise
Genomics Services @ GENterprise since 1998 Mainz University spin-off privately financed 6-10 employees since 2006 Genomics Services @ GENterprise Sequencing Service (Sanger/3730, 454) Genome Projects (Bacteria,
More informationDNA Sequence Analysis
DNA Sequence Analysis Two general kinds of analysis Screen for one of a set of known sequences Determine the sequence even if it is novel Screening for a known sequence usually involves an oligonucleotide
More informationEssentials of Real Time PCR. About Sequence Detection Chemistries
Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected
More informationHBV PCR detection Kit USER MANUAL
For professional use only HBV PCR detection Kit (PREP-NA DNA/RNA Extraction Kit included) USER MANUAL "DNA-Technology, Research & Production" LLC Russia, 142281, Moscow Region, Protvino, 2 Zheleznodorozhnaya
More informationFabrication Challenges for Point-ofcare Diagnostics and Organ-on-chip
Fabrication Challenges for Point-ofcare Diagnostics and Organ-on-chip Zulfiqur Ali (z.ali@tees.ac.uk) Project DIGINOVA: Biomedical Applications for Digital Fabrication, 6 th November 2013, CPI s National
More informationThermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual
Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual F- 470S 20 cdna synthesis reactions (20 µl each) F- 470L 100 cdna synthesis reactions (20 µl each) Table of contents 1. Description...
More informationElectronic Biosensors Henrique Leonel Gomes hgomes@ualg.pt Office: 2.82 Phone: +351 289 800 900 ext. 7763 Fax: +351 289 8000 066
Electronic Biosensors Henrique Leonel Gomes hgomes@ualg.pt Office: 2.82 Phone: +351 289 800 900 ext. 7763 Fax: +351 289 8000 066 Welcome Welcome to electronic biosensors course. This is a useful course
More informationMolecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION
Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION Susan Carson Heather B. Miller D.Scott Witherow ELSEVIER AMSTERDAM BOSTON HEIDELBERG LONDON NEW YORK OXFORD PARIS SAN DIEGO SAN
More informationIntroduction to next-generation sequencing data
Introduction to next-generation sequencing data David Simpson Centre for Experimental Medicine Queens University Belfast http://www.qub.ac.uk/research-centres/cem/ Outline History of DNA sequencing NGS
More informationAppendix 2 Molecular Biology Core Curriculum. Websites and Other Resources
Appendix 2 Molecular Biology Core Curriculum Websites and Other Resources Chapter 1 - The Molecular Basis of Cancer 1. Inside Cancer http://www.insidecancer.org/ From the Dolan DNA Learning Center Cold
More informationAgilent in Life Sciences > Genomics > Proteomics > Drug Discovery > Development > QA/QC. Agilent 2100 Bioanalyzer Automated Analysis System
Agilent in Life Sciences > Genomics > Proteomics > Drug Discovery > Development > QA/QC Agilent 2100 Bioanalyzer Automated Analysis System The ideal companion in life science Lab-on-a-chip Accelerating
More informationGenomic DNA Clean & Concentrator Catalog Nos. D4010 & D4011
Page 0 INSTRUCTION MANUAL Catalog Nos. D4010 & D4011 Highlights Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)dna, etc.)
More informationID kit. imegen Anchovies II. and E. japonicus) DNA detection by. User manual. Anchovies species (E. encrasicolus. sequencing.
User manual imegen Anchovies II ID kit Anchovies species (E. encrasicolus and E. japonicus) DNA detection by sequencing Reference: Made in Spain The information in this guide is subject to change without
More informationTraditional or conventional sequencing consists mainly in the combination of a modified PCR procedure (the sequencing reaction) and a highresolution
46 - Introduction Sequencing Sequencing is the primma dona of the analytical techniques the molecular geneticist has at hand. Not only does it provide the ultimate information on the DNA molecule (sc.
More informationGeneXpert Technology. Indira Soundiram 2012
GeneXpert Technology Indira Soundiram 2012 A Better Way to Platform Design GeneXpert Infinity-48 GeneXpert Module GX-I GX-II GX-IV GX-XVI 2 Defining Molecular Diagnostics Any Test Any Time Any Sample Any
More informationCoupling Engineering Sciences and Life Sciences: An overview of LAAS Activities with a special focus on Nano-Oncology Projects
Christophe Vieu Toulouse University Chargé de Mission du LAAS Axe Interaction avec le vivant Coupling Engineering Sciences and Life Sciences: An overview of LAAS Activities with a special focus on Nano-Oncology
More informationRealLine HCV PCR Qualitative - Uni-Format
Instructions for use PCR KIT FOR EXTRACTION OF RNA AND REAL TIME PCR DETECTION KIT FOR HEPATITIS C VIRUS RNA Research Use Only Qualitative Uni Format VBD0798 48 tests valid from: December 2013 Rev11122013
More informationHow many of you have checked out the web site on protein-dna interactions?
How many of you have checked out the web site on protein-dna interactions? Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Find and be ready to discuss
More informationSix Trends in Robotics in the Life Sciences
Parker Hannifin Electromechanical Dvision N. A. 1140 Sandy Hill Road Irwin, PA 1564203049 724-861-6903 www.parkermotion.com Six Trends in Robotics in the Life Sciences By Mike Szesterniak Big Impact in
More informationReal-time monitoring of rolling circle amplification using aggregation-induced emission: applications for biological detection
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 215 Supplementary Information Real-time monitoring of rolling circle amplification using aggregation-induced
More informationWhy silicon MEMS? MEMS@KTH. Silicon is a strong material... Photolithography. Micromachining. Dicing and packaging
Why silicon MEMS? MEMS@KTH Small Identical Large volumes (low cost per unit) School of Electrical Engineering Royal Institute of Technology Silicon is a strong material... Photolithography 10 µm thick
More informationAll-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna
All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna All-in-One TM mirna First-Strand cdna Synthesis Kit AMRT-0020 (20 RT reactions), AMRT-0060 (60 RT reactions) Used in combination
More informationLabor im Zentrifugenröhrchen zur dezentralen patientennahen Diagnostik
Labor im Zentrifugenröhrchen zur dezentralen patientennahen Diagnostik Felix von Stetten HSG-IMIT, Georges Köhler Alle 103, Freiburg, Germany 13.11.2013 - Mobile Diagnostik am Point-of-Care, DGBMT, Düsseldorf
More informationHow is genome sequencing done?
How is genome sequencing done? Using 454 Sequencing on the Genome Sequencer FLX System, DNA from a genome is converted into sequence data through four primary steps: Step One DNA sample preparation; Step
More informationVLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J10
Topic J10+11: Molecular-biological methods + Clinical virology I (hepatitis A, B & C, HIV) To study: PCR, ELISA, your own notes from serology reactions Task J10/1: DNA isolation of the etiological agent
More informationBio@IT Lisboa, July 2, 2012. Bioelectronics. Luís Alcácer. 2005, it - instituto de telecomunicações. Todos os direitos reservados.
Lisboa, July 2, 2012 Luís Alcácer 2005, it - instituto de telecomunicações. Todos os direitos reservados. Outline Organic Organic Nanoelectronics Molecular Electronics 2 July 2, 2012 Lisboa Sensu lato
More informationTable of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3
Table of Contents I. Description... 2 II. Kit Components... 2 III. Storage... 2 IV. 1st Strand cdna Synthesis Reaction... 3 V. RT-PCR, Real-time RT-PCR... 4 VI. Application... 5 VII. Preparation of RNA
More informationSYBR Green Realtime PCR Master Mix -Plus-
Instruction manual SYBR Green Realtime PCR Master Mix -Plus- 0810 F0925K SYBR Green Realtime PCR Master Mix -Plus- Contents QPK-212T 1mLx1 QPK-212 1mLx5 Store at -20 C, protected from light [1] Introduction
More informationHISTO SPOT SSO System
HISTO SPOT SSO System for HLA Typing HISTO SPOT Kits MR.SPOT Processor HISTO MATCH Software Complete system certified for IvD use HISTO SPOT Features The HISTO SPOT SSO assay in combination with the MR.SPOT
More informationGenetic Analysis. Phenotype analysis: biological-biochemical analysis. Genotype analysis: molecular and physical analysis
Genetic Analysis Phenotype analysis: biological-biochemical analysis Behaviour under specific environmental conditions Behaviour of specific genetic configurations Behaviour of progeny in crosses - Genotype
More informationAdvances in RainDance Sequence Enrichment Technology and Applications in Cancer Research. March 17, 2011 Rendez-Vous Séquençage
Advances in RainDance Sequence Enrichment Technology and Applications in Cancer Research March 17, 2011 Rendez-Vous Séquençage Presentation Overview Core Technology Review Sequence Enrichment Application
More informationJeong-Yeol Yoon. Introduction to Biosensors. From Electric Circuits to Immunosensors. f) Springer
Jeong-Yeol Yoon Introduction to Biosensors From Electric Circuits to Immunosensors f) Springer Contents 1 Introduction 1 1.1 Sensors 1 1.2 Transducers 2 1.3 Biosensors 4 1.4 Bioreceptors 5 1.5 Transducers
More informationContents. Microfluidics - Jens Ducrée Physics: Fluid Dynamics 1
Contents 1. Introduction 2. Fluids 3. Physics of Microfluidic Systems 4. Microfabrication Technologies 5. Flow Control 6. Micropumps 7. Sensors 8. Ink-Jet Technology 9. Liquid Handling 10.Microarrays 11.Microreactors
More informationMicroarray Technology
Microarrays And Functional Genomics CPSC265 Matt Hudson Microarray Technology Relatively young technology Usually used like a Northern blot can determine the amount of mrna for a particular gene Except
More informationNanotechnology, Biological Engineering and the Future of Biosensors
1 Nanotechnology, Biological Engineering and the Future of Biosensors Lesson Overview: Nanotechnologies allow the digital world and the biological world to merge and can therefore detect biological substances.
More informationPROVIDING TECHNICAL SOLUTIONS FOR BIOLOGICAL PROCESSES AND MEDICAL DEVICES
PROVIDING TECHNICAL SOLUTIONS FOR BIOLOGICAL PROCESSES AND MEDICAL DEVICES ABOUT LIFE SCIENCES ENGINEERING The joint Life Sciences Engineering business unit of Fraunhofer IPT and CMI has offices in Boston,
More informationRapid DNA Analysis DNA: the Ultimate Biometric? Advances in Molecular Processing and Analysis
Rapid DNA Analysis DNA: the Ultimate Biometric? Advances in Molecular Processing and Analysis Presentation at NSF Workshop on Fundamental Research Challenges for Trustworthy Biometrics Dr. Joan Bienvenue
More informationUser Manual. CelluLyser Lysis and cdna Synthesis Kit. Version 1.4 Oct 2012 From cells to cdna in one tube
User Manual CelluLyser Lysis and cdna Synthesis Kit Version 1.4 Oct 2012 From cells to cdna in one tube CelluLyser Lysis and cdna Synthesis Kit Table of contents Introduction 4 Contents 5 Storage 5 Additionally
More informationIdentification of the VTEC serogroups mainly associated with human infections by conventional PCR amplification of O-associated genes
Identification of the VTEC serogroups mainly associated with human infections by conventional PCR amplification of O-associated genes 1. Aim and field of application The present method concerns the identification
More informationIntroduction to Flow Cytometry
Outline Introduction to Flow Cytometry Basic Concept of Flow Cytometry Introduction to Instrument Subsystems Daisy Kuo Assistant Product Manager E-mail: daisy_kuo@bd.com BDBiosciences Application Examples
More informationHBV Quantitative Real Time PCR Kit
Revision No.: ZJ0002 Issue Date: Aug 7 th, 2008 HBV Quantitative Real Time PCR Kit Cat. No.: HD-0002-01 For Use with LightCycler 1.0/LightCycler2.0/LightCycler480 (Roche) Real Time PCR Systems (Pls ignore
More informationab185916 Hi-Fi cdna Synthesis Kit
ab185916 Hi-Fi cdna Synthesis Kit Instructions for Use For cdna synthesis from various RNA samples This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 1
More informationIntroduction to flow cytometry
Introduction to flow cytometry Flow cytometry is a popular laser-based technology. Discover more with our introduction to flow cytometry. Flow cytometry is now a widely used method for analyzing the expression
More informationmircute mirna qpcr Detection Kit (SYBR Green)
mircute mirna qpcr Detection Kit (SYBR Green) For detection of mirna using real-time RT-PCR (SYBR Green I) www.tiangen.com QP110302 mircute mirna qpcr Detection Kit (SYBR Green) Kit Contents Cat. no. FP401
More informationRealStar HBV PCR Kit 1.0 11/2012
RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 For research use only! (RUO) Product No.: 201003 96 rxns INS-201000-GB-02 Store at -25 C... -15 C November 2012 altona Diagnostics GmbH Mörkenstraße
More informationThe Techniques of Molecular Biology: Forensic DNA Fingerprinting
Revised Fall 2011 The Techniques of Molecular Biology: Forensic DNA Fingerprinting The techniques of molecular biology are used to manipulate the structure and function of molecules such as DNA and proteins
More informationBeginner s Guide to Real-Time PCR
Beginner s Guide to Real-Time PCR 02 Real-time PCR basic principles PCR or the Polymerase Chain Reaction has become the cornerstone of modern molecular biology the world over. Real-time PCR is an advanced
More informationBacReady TM Multiplex PCR System
BacReady TM Multiplex PCR System Technical Manual No. 0191 Version 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 2 V Simplified Procedures. 2 VI Detailed Experimental
More informationAn Overview of DNA Sequencing
An Overview of DNA Sequencing Prokaryotic DNA Plasmid http://en.wikipedia.org/wiki/image:prokaryote_cell_diagram.svg Eukaryotic DNA http://en.wikipedia.org/wiki/image:plant_cell_structure_svg.svg DNA Structure
More informationFACULTY OF MEDICAL SCIENCE
Doctor of Philosophy in Biochemistry FACULTY OF MEDICAL SCIENCE Naresuan University 73 Doctor of Philosophy in Biochemistry The Biochemistry Department at Naresuan University is a leader in lower northern
More informationNext Generation Sequencing
Next Generation Sequencing DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection Over the past three years, massively
More informationValidating Microarray Data Using RT 2 Real-Time PCR Products
Validating Microarray Data Using RT 2 Real-Time PCR Products Introduction: Real-time PCR monitors the amount of amplicon as the reaction occurs. Usually, the amount of product is directly related to the
More informationMobile Lab-Diagnostik
Mobile Lab-Diagnostik Jenaer Technologietag 2013 Alere Technologies GmbH, Jena; 11.11.2013, Torsten Schulz ALERE TECHNOLOGIES Alere Technologies GmbH former CLONDIAG GmbH 1998/1999 Foundation; 2001 first
More informationLecture 13: DNA Technology. DNA Sequencing. DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology
Lecture 13: DNA Technology DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology DNA Sequencing determine order of nucleotides in a strand of DNA > bases = A,
More informationMitos P-Pump. product datasheet
product datasheet page Product description 2 Main benefits 3 Why choose the Mitos P-Pump? 3 Closed-loop flow control 4 Product specifications 5 Accessories - overview 6 Mitos P-Pump Basic 7 Mitos P-Pump
More informationBiotracker TM A Laboratory Information Management System By Ocimum Biosolutions
Biotracker TM A Laboratory Information Management System By Ocimum Biosolutions 1 TABLE OF CONTENTS 1.0 EXECUTIVE SUMMARY... 2 2.0 INTRODUCTION... 2 3.0 BIOTRACKER TM GENERAL FEATURES... 4 3.1 LABORATORY
More informationBiomedical Engineering Graduate Program in METU / ANKARA
Biomedical Engineering Graduate Program in METU / ANKARA Faculty of Engineering Institute of Applied Mathematics Informatics Institute Faculty of Arts and Sciences Electrical and Electronics and Engineering
More informationZR DNA Sequencing Clean-up Kit
INSTRUCTION MANUAL ZR DNA Sequencing Clean-up Kit Catalog Nos. D40 & D4051 Highlights Simple 2 Minute Bind, Wash, Elute Procedure Flexible 6-20 µl Elution Volumes Allow for Direct Loading of Samples with
More informationHow To Get A Cell Print
QUICK CELL CAPTURE AND CHARACTERIZATION GUIDE FOR CELLSEARCH CUSTOMERS CellSave EDTA Blood sample Rare cell capture Enumeration Single protein marker Cell capture for molecular characterization CELLSEARCH
More informationUNIVERSITY OF PÉCS MEDICAL SCHOOL FLOW CYTOMETRY AND CELL SEPARATION BIOPHYSICS 2. 2015 4th March Dr. Beáta Bugyi Department of Biophysics Flow cytometry and cell separation FLOW = STREAM OF FLUID in a
More informationQPCR Applications using Stratagene s Mx Real-Time PCR Platform
QPCR Applications using Stratagene s Mx Real-Time PCR Platform Dan Schoeffner, Ph.D Field Applications Scientist Dan.Schoeffner@Stratagene.com Tech. Services 800-894-1304 Polymerase Chain Reaction Melt
More informationPrimeSTAR HS DNA Polymerase
Cat. # R010A For Research Use PrimeSTAR HS DNA Polymerase Product Manual Table of Contents I. Description...3 II. III. IV. Components...3 Storage...3 Features...3 V. General Composition of PCR Reaction
More informationWelcome to Pacific Biosciences' Introduction to SMRTbell Template Preparation.
Introduction to SMRTbell Template Preparation 100 338 500 01 1. SMRTbell Template Preparation 1.1 Introduction to SMRTbell Template Preparation Welcome to Pacific Biosciences' Introduction to SMRTbell
More informationAurora Forensic Sample Clean-up Protocol
Aurora Forensic Sample Clean-up Protocol 106-0008-BA-D 2015 Boreal Genomics, Inc. All rights reserved. All trademarks are property of their owners. http://www.borealgenomics.com support@borealgenomics.com
More informationMagExtractor -Genome-
Instruction manual MagExtractor-Genome-0810 F0981K MagExtractor -Genome- NPK-101 100 preparations Store at 4 C Contents [1] Introduction [2] Components [3] Materials required [4] Protocol 1. Purification
More informationHiPer RT-PCR Teaching Kit
HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The
More informationFraunhofer Institute for Material and Beam Technology
Laser Micro Processing Dr. Udo Klotzbach Fraunhofer Institute for Material and Beam Technology Dresden Overview Equipment - CO 2 -lasers - Solid state lasers (Nd:YAG, Disk, Fiber) - Diode lasers - Excimer
More informationGenomic DNA Extraction Kit INSTRUCTION MANUAL
Genomic DNA Extraction Kit INSTRUCTION MANUAL Table of Contents Introduction 3 Kit Components 3 Storage Conditions 4 Recommended Equipment and Reagents 4 Introduction to the Protocol 4 General Overview
More informationSpeed Matters - Fast ways from template to result
qpcr Symposium 2007 - Weihenstephan Speed Matters - Fast ways from template to result March 28, 2007 Dr. Thorsten Traeger Senior Scientist, Research and Development - 1 - Overview Ạgenda Fast PCR The Challenges
More informationTroubleshooting Sequencing Data
Troubleshooting Sequencing Data Troubleshooting Sequencing Data No recognizable sequence (see page 7-10) Insufficient Quantitate the DNA. Increase the amount of DNA in the sequencing reactions. See page
More informationReverse Transcription System
TECHNICAL BULLETIN Reverse Transcription System Instruc ons for use of Product A3500 Revised 1/14 TB099 Reverse Transcription System All technical literature is available on the Internet at: www.promega.com/protocols/
More informationChIP TROUBLESHOOTING TIPS
ChIP TROUBLESHOOTING TIPS Creative Diagnostics Abstract ChIP dissects the spatial and temporal dynamics of the interactions between chromatin and its associated factors CD Creative Diagnostics info@creative-
More informationMir-X mirna First-Strand Synthesis Kit User Manual
User Manual Mir-X mirna First-Strand Synthesis Kit User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories, Inc.
More informationHISTO SPOT SSO System. The most convenient automated HLA typing system. BAG Health Care the experts for HLA and blood group diagnostics
HISTO SPOT SSO System The most convenient automated HLA typing system BAG Health Care the experts for HLA and blood group diagnostics HISTO SPOT SSO System for on call, high throughput and disease association
More informationwww.gbo.com/bioscience Tissue Culture 1 Cell/ Microplates 2 HTS- 3 Immunology/ HLA 4 Microbiology/ Bacteriology Purpose Beakers 5 Tubes/Multi-
10 Biochips / Biochips Introduction 10 I 2 Diagnostic Kits 10 I 4 PapilloCheck 10 I 4 PapilloCheck high-risk 10 l 4 PapilloCheck Collection Kit 10 l 4 ParoCheck 10 I 5 CarnoCheck 10 I 6 ocheck DNA Extraction
More informationM.Sc. in Nano Technology with specialisation in Nano Biotechnology
M.Sc. in Nano Technology with specialisation in Nano Biotechnology Nanotechnology is all about designing, fabricating and controlling materials, components and machinery with dimensions on the nanoscale,
More informationMethylation Analysis Using Methylation-Sensitive HRM and DNA Sequencing
APPLICATION NOTE Methylation Analysis Using Methylation-Sensitive HRM and DNA Sequencing Methylation Analysis Using Methylation Sensitive HRM and DNA Sequencing Abstract DNA methylation is a key epigenetic
More informationApplication Guide... 2
Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue Application Guide... 2 I. Description... 2 II. Product Components... 2 III. Materials to be Supplied
More informationQuantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit
Product Bulletin Human Identification Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit The Quantifiler kits produce reliable and reproducible results, helping to
More informationIII. MEMS Projection Helvetica 20 Displays
Helvetica 26 Helvetica 22 III. MEMS Projection Displays Micro Mirror Projection - Texas Instruments DMD - Daewoo Elec. AMA Grating Light Valve - Silicon Light Machines Image Projection Color Synthesis
More informationHighly specific and sensitive quantitation
PRODUCT ULLETIN SYR Select Master Mix SYR Select Master Mix Highly specific and sensitive quantitation SYR Select Master Mix offers advanced performance at an affordable price. SYR Select Master Mix is
More informationGene Expression Assays
APPLICATION NOTE TaqMan Gene Expression Assays A mpl i fic ationef ficienc yof TaqMan Gene Expression Assays Assays tested extensively for qpcr efficiency Key factors that affect efficiency Efficiency
More informationCABIOS. Q-RT-PCR: data analysis software for measurement of gene expression by competitive RT-PCR
CABIOS Vol. 73 no. 6 1997 Pages 587-591 Q-RT-PCR: data analysis software for measurement of gene expression by competitive RT-PCR Peter A. Doris 2, Amanda Hayward-Lester and Jon K. Hays Sr 1 Department
More informationMolecular Genetics: Challenges for Statistical Practice. J.K. Lindsey
Molecular Genetics: Challenges for Statistical Practice J.K. Lindsey 1. What is a Microarray? 2. Design Questions 3. Modelling Questions 4. Longitudinal Data 5. Conclusions 1. What is a microarray? A microarray
More informationOligonucleotide Stability Study
Oligonucleotide Stability Study IDT is performing a long term study of oligo stability. The study has been ongoing for 2 years and has tested oligo stability under various conditions. An overview of the
More informationABSTRACT. Promega Corporation, Updated September 2008. http://www.promega.com/pubhub. 1 Campbell-Staton, S.
A Modified Wizard SV Genomic DNA Purification System Protocol to Purify Genomic DNA... A Modified Wizard SV Genomic DNA Purification System Protocol to Purify Genomic DNA from Shed Reptile Skin ABSTRACT
More informationMEMS and Sensor Trends Smaller, Faster and Available to the Mass Market. Karen Lightman, Executive Director MEMS Industry Group
MEMS and Sensor Trends Smaller, Faster and Available to the Mass Market Karen Lightman, Executive Director MEMS Industry Group Uplinq Hardware Day - 2013 About MEMS Industry Group (MIG) MIG is the industry
More informationIntroduction. Preparation of Template DNA
Procedures and Recommendations for DNA Sequencing at the Plant-Microbe Genomics Facility Ohio State University Biological Sciences Building Room 420, 484 W. 12th Ave., Columbus OH 43210 Telephone: 614/247-6204;
More informationMolecular Computing. david.wishart@ualberta.ca 3-41 Athabasca Hall Sept. 30, 2013
Molecular Computing david.wishart@ualberta.ca 3-41 Athabasca Hall Sept. 30, 2013 What Was The World s First Computer? The World s First Computer? ENIAC - 1946 Antikythera Mechanism - 80 BP Babbage Analytical
More informationCell Discovery 360: Explore more possibilities.
Cell Discovery 360: Explore more possibilities. Flexible solutions for the BioPharma laboratory Blood Banking Capillary Electrophoresis Centrifugation Flow Cytometry Genomics Lab Automation Lab Tools Particle
More informationbitter is de pil Linos Vandekerckhove, MD, PhD
4//24 Current HIV care HIV copies/ ml plasma Viral load Welcome to the Digital droplet PCR age! bitter is de pil Linos Vandekerckhove, MD, PhD Latent HIV reservoir Time at Ghent University Hospital 2 HIV
More informationEurotraining survey on Microsytems training requirements
Eurotraining survey on Microsytems training requirements Hervé Fanet CEA LETI Annette Locher FSRM Chantal Tardif CEA INSTN Abstract One objective of the Eurotraining MST project is to identify training
More informationLeti Introduction and Overview
Leti Introduction and Overview Leti Day in Tokyo, October 3rd 2012 Laurent Malier 2012 Leti 2012 1 CEA The CEA at a glance Commissariat à l Énergie Atomique et aux Énergies Alternatives is one of the largest
More informationDyNAmo cdna Synthesis Kit for qrt-pcr
DyNAmo cdna Synthesis Kit for qrt-pcr Instruction manual F- 470S Sufficient for 20 cdna synthesis reactions (20 µl each) F- 470L Sufficient for 100 cdna synthesis reactions (20 µl each) Description...
More information