Liquid Chromatograph Mass Spectrometer LCMS-2020 C146-E121E

Transcription

1 Liquid hromatograph Mass Spectrometer LMS E121E

2 LMS-2020 Seeing is elieving. Ultra Fast UFswitching Rapid 15 msec Polarity Switching Ultra Fast UFsensitivity Superior Sensitivity from UFL Ultra Fast UFscanning 15,000 u/sec Fast Scanning Speed

3 GMS-QP2010 Ultra GMS-QP2010 SE GMS-TQ8030 LMS-8030 LMS-8040 LMS-8080 LMS-2020 LMS-IT-TOF

4 UFL Quality. From HPL to UFL. Then to UFL/MS. reservior pump auto sampler column oven detector From HPL to UFL UFL achieves excellent speed and resolution, while offering the high precision not available with conventional HPL and expandability options. Ultra Fast Not only high-speed analysis, but increased overall speed through rapid sample injection and fully automatic analysis functions. Unquestionable Fidelity UFL offers exceptional injection reproducibility as well as ultra high-speed operation. In terms of minimizing sample carryover, essential in L/MS analysis, the LMS-2020 stays ahead of the competition. Ultra Flexible overs an extensive range from ultra-fast analysis to conventional HPL and semi-preparative analysis. 4

5 Ultra Fast UFsensitivity UFswitching UFscanning LMS-2020 Liquid hromatograph Mass Spectrometer 5

6 Speed is Power. Greater speed. Greater sensitivity. Ultra-fast UFswitching Rapid 15-millisecond positive/negative ionization switching To detect both positive and negative ions, analysis is performed while switching between the positive and negative ionization modes. The LMS-2020 adopts patented high-voltage power supply technology (Patent: US ) to achieve an ultra-fast polarity switching time of just 15 ms. Polarity switching time 15 msec Polarity switching time 15 msec Positive-ion measurement SIM 2H Negative-ion measurement SIM 2H Positive-ion measurement SIM 2H Sample: 321: hloramphenicol 344: Dibucaine 329: Furosemide 231: Isopropylantipyline 6

7 ccurate mass analysis of sharp chromatographic peaks obtained by UFL requires ultra-fast MS detection capabilities. The LMS-2020 offers UFswitching for rapid switching between the positive and negative ionization modes and UFscanning for ultra-fast scan measurements to capture the sharpest UFL peaks. Ionization Probe Desolvation Line Skimmer Entrance Lens Pre-rod Main-rod Detector Enhances sensitivity by preventing ion divergence Ion Source Qarray Octopole Quadrupole Ultra-fast UFsensitivity Superior sensitivity from UFL The newly developed Qarray Optics achieves superior sensitivity, reproducibility, and linearity. Ultra-fast UFscanning 15,000 u/sec fast scanning speed ontrols the voltage applied to the Quadrupole according to the scan speed and. Shimadzu's proprietary scanning technology (Patent: US ) maintains resolution and achieves high ion transmittance even at high scanning speeds. alibration curve 414: L-α-Narcotine 256: Diphenhydramine 267: Desipramine 278: mitriptyline LMS-2020 Liquid hromatograph Mass Spectrometer 7

8 UFscanning & UFswitching Necessity of UFswitching and UFscanning for ultra-fast analysis Ultra-fast detection (MS measurement) is required for ultra-fast analysis with elution of six components per minute, for example. The UFswitching and UFscanning functions permit the required ultra-fast mass spectrometry. Polarity switching time 15 msec Polarity switching time 15 msec Positive-ion measurement 15,000 u/sec Negative-ion measurement 15,000 u/sec Positive-ion measurement 15,000 u/sec Examples of ionization in positive and negative modes MS Spectra of entazone MS Spectra of Dymuron MS Spectra of arpropamid 8

9 Hardware Features that to Powerfully Support Three UFs Toughness against dirty samples In order to check the toughness of the LMS-2020 against dirty samples, plasma samples simply precipitated with only acetonitrile were injected 2,500 times over 10 days (1 µl volume per injection). Excellent reproducibility of peak area was demonstrated and its RSD was 2.26%. Easy Maintenance The desolvation line (DL) that introduces the sample from the ion source into the vacuum can be installed and uninstalled without breaking the vacuum, which dramatically enhances ease-of-maintenance. DL Vacuum region %RSD2.26 Internal standard nalysis time 6 min 2500 injections over 10 days Retaining the vacuum even if the DL is uninstalled reating Fragment Ions by In-source ID In-source ID (collision-induced dissociation) is effective for confirming the molecular weight of synthetic compounds and for the quantification of impurities. MS hromatogram for Erythromycin Measurements DL=0V, Qarray D=0V Magnified view Erythromycin (major component) Using in-source ID allows the generation of fragment ions. This example shows the structure of impurities in erythromycin estimated from fragment ions generated by in-source ID. The multi-sequence mode permits several other methods within a single analysis, such as ID, positive/negative ion switching modes, and SN/SIM modes. Precisely setting the parameters reduces the risk of erroneous evaluations and enhances the reliability of analysis results. Erythromycin (major component) MS Spectra (Normal mode) DL=0V, Qarray D=0V MS Spectra (In-source ID mode) DL=0V, Qarray D=60V Erythromycin (major component) Peak1 85-(8)-Hydroxy-Erythromycin LMS-2020 Liquid hromatograph Mass Spectrometer 9

10 Diverse Ionization Methods Expand the Range of pplications Selecting the ionization method appropriate for the target compound achieves superior analysis results. LMS-2020 offers PI and DUIS in addition to ESI. Diverse ionization methods support a wide range of applications. Selecting the most appropriate Ionization Method ompounds DN Proteins Functional groups cid lcohol Peptides ldehyde mino acids lkane Macromolecules lkyne arbohydrates mino Triglycerides arbonyl romatic hydrocarbons Ester liphatic hydrocarbons Ether Properties Polar Phenol Non-polar Volatile Extremely suitable Non-volatile Suitable Thermostable nalysis possible with appropriate parameters Thermolabile Inherently unsuitable Ionization Options While the water-soluble vitamins thiamine and riboflavin can be detected by ESI, they are virtually undetectable by PI. onversely, the fat-soluble vitamin calciferol can be detected by PI but ESI does not offer adequate detection sensitivity. DUIS-2020 allows detection of a mixture of compounds suited to ESI or PI, without missing any compounds. PI-2020 Sample injection MS hromatograms orona needle MS Spectra from DUIS Measurements DUIS-2020 ESI probe orona needle Thiamine Riboflavin alciferol Mixed Sample of Three Water-soluble/Fat-soluble Vitamins 1. Thiamine: 265; cation, generated by ionization, water-soluble vitamin 2. Riboflavin: 377; protonated molecule, water-soluble vitamin 3. alciferol: 397; protonated molecule, fat-soluble vitamin 10

11 LabSolutions LMS Powerful support for UFL/LMS-2020 high-speed performance. This software maximizes analysis performance. Rapidly analyzes huge volumes of data in browser windows. The comprehensive, clear display provides a stress-free working environment. Manual peak integration bar Peak table MS spectrum tabel ontrol Target omparison of ontrol and Target Multiple data items are displayed sequentially on the same screen. To view the diverse information in a data file in the optimal layout for comparison, the data can be browsed like flipping through the pages of a book to discover differences between the data. Peak Integration Manual peak integration can be conducted on both L and MS chromatograms simultaneously. oth the peak table and MS spectrum table are displayed. The peak table and chromatograms/spectra are interlinked for easier operation. Optimization of nalysis Parameters utomatically searches and sets the voltages that affect the ion transmittance (DL/Qarray voltage) to the optimal values for the target compounds. SIM Table Enter the target Method Optimization Window Specify the conditions to search for the optimal voltage utomatic analysis execution Method Optimization Result Window The Search results of the optimal voltage value are displayed nalytical conditions are optimized SIM Table The optimal voltage value is set into the SIM table automatically LMS-2020 Liquid hromatograph Mass Spectrometer 11

12 Open Solution nalytical daptive open access software Multiple options for sample log-in (Wizard, Table or Simple sample log-in mode) dvanced column management support (ph switching, column washing and parking) File management (including copy locations) Instrument use (including night time operation, sleep mode) Method management (advanced options for pre- and post-run options Global sample and instrument status and message board feedback Log-in options an be configured for user name, password (or none) Time remaining Large display for clarity Status olor coded updates Select Method dd description Name can be automatically generated Message board Updates system messages 2 Steps only! lick to submit and finish Sample queue analysis 12

13 Dynamic data review experience Data review can be launched on any remote P without software installation. lick on the link in an and the data will be shown. Supports multiple data presentations (including 2 panels for data comparison) for simple, clear data review. dvanced users can reprocess raw data or quickly review processed XML results. Spectral integrity; unique mode for checking for co-elution. utomatically checks mass spectral data across a detected peak. Spectral integrity score Highlights possible co-elution opy/paste Text Structure display Pasted structure Target views Each target colored green, yellow or red Intelligent mass spectrum labelling Target ions labelled in the mass spectrum No installation Only requires free Microsoft components Scalability Number of user licenses can be easily expanded LN Laboratory UFL LMS LMS-2020 Office LMS-2020 Liquid hromatograph Mass Spectrometer 13

14 LMS-2020 ompany names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu orporation or its affiliates, whether or not they are used with trademark symbol TM or. Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own. For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you as is without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication. Shimadzu orporation, 2014 Printed in Japan NS