The world's largest elearning website for analytical scientists

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1 The world's largest elearning website for analytical scientists CHROMacademy will help increase your knowledge, efficiency and productivity in the lab HPLC GC MS SPE IR BLS BIO

2 HPLC GC MS SPE IR BLS BIO A CHROMacademy Premier Membership provides you with complete access to all content including: 1000 s of elearning Topics HPLC / GC / Sample Prep / MS/ Infrared / Basic Lab Skills* / Biochromatography* / * coming in 2015 The Essential Guide Webcast & Tutorial Archive Over 70 training topics covered by industry experts HPLC & GC Troubleshooting and Virtual Lab Tools Become the lab expert with our HPLC and GC troubleshooters 24hr Chromatography Support Ask the Expert - our experts will answer your questions within 24 hours 6 New Video Training Courses Fundamentals of HPLC / GCMS / GC / LCMS Method Development - HPLC & GC Assessments Test your knowledge, certificates awarded upon completion User Forum Communicate with others interested in analytical science Application Notes & Articles The latest application notes & LCGC articles Lite members have access to less than 5% of our content... Premier members get so much more

3 HPLC GC MS SPE IR BLS BIO e-learning for analytical scientists HPLC GC MS SPE IR BLS BIO e-learning for analytical scientists Packed with practical information that will help improve your skills and productivity Used by over 35,000 chromatographers worldwide, CHROMacademy will help increase your knowledge, efficiency and productivity in the lab. With a vast library of high-quality animated and interactive e-learning topics, webcasts, tutorials and troubleshooting tools we can help you refresh your chromatography skills or learn something completely new. CHROMacademy is simply the best training tool for analytical scientists - see for yourself!

4 HPLC GC MS Sample Prep Infrared Basic Lab Skills* Biochromatography* * coming in 2015 Each channel is packed with e-learning modules, webcasts, tutorials and interactive tools

5 High Performance Liquid Chromatography Injection Valve Anatomyy It is necessary to divert the flow of mobile phase away from the sampling system (i.e. the syringe) when aspirating the sample and / or filling the sample loop prior to injection. This is achieved using the injection valve containing a Rotor Seal. The interface between the HPLC capillaries and the stator is known as the Stator Face... Isolation Seal Rotor Seal THE THEORY OF HPLC Introduction to HPLC Chromatographic Parameters Band Broadening Column chemistry Reverse phase (partition) chromatography Ion-Pair Chromatography Normal phase (absorption) chromatography Gradient HPLC Quantitative and Qualitative HPLC Fast HPLC HILIC Supercritical Fluid Chromatography Introduction to Ion Chromatography Stator Face Stator INSTRUMENTATION OF HPLC Mobile Phase Considerations HPLC Solvent Pumping Systems Autosamplers Detectors

6 Gas Chromatography THEORY AND INSTRUMENTATION OF GC Introduction to GC Chromatographic Parameters Band Broadening Gas Supply and Pressure Control Sampling Techniques Sample Introduction GC Columns GC Temperature Programming GC Detectors Supercritical Fluid Chromatography 1. Aims & Objectives 2. Introduction 3. Supercritical Fluids 4. Modes of Chromatography 5. SFC Applications 6. SFC Instrumentation 7. Packed & Capillary SFC 8. The Mobile Phase 9. Exhaust Gases 10. Organic Modifiers 11. Pumping Issues 12. SFC Columns 13. SFC Stationary Phases & Column Selection 14. Detection 15. Pressure Regulators 16. Advantages and Disadvantages of SFC

7 Mass Spectrometry FUNDAMENTAL LC-MS +M M Non-Volatile Analyte is Charged in Solution Nebulising Gas e+ e + e + e + e + e + + e e + e M + + M + M + Me M + M M e + e Corona Electrode Pin Introduction Electro Spray Ionization Theory Electro Spray Ionization Instrumentation Mass Analyzers Atmospheric Pressure Chemical Ionization (APCI) Atmospheric Pressure Photo Ionization (APPI) Solvents, Buffers and Additives Vacuum Systems Flow Rates and Flow Splitting Orbitrap Mass Analyzers FUNDAMENTAL GC-MS Introduction GC Considerations GC -MS Interfaces MS INTERPRETATION General Interpretation Strategies Fundamentals of MS Proteomics Research

8 Sample Preparation SOLID PHASE EXTRACTION Molecular Properties 1. Learning Aims & Objectives 2. Functional groups 3. Molecular Properties 4. Functional group interactions 5. Hydrophobic or Non-Polar Groups 6. Hydrophobic Interactions -Solubility 7. Hydrophobic Interactions -Sorbents 8. Polar Groups 9. Polar Interactions -Solubility 10. Polar Interactions -Sorbents 11. Ionic groups 12. Ionic groups -ph Ka pka Ionic strength 13. Ionic Interactions -Solubility Sorbents 14. Ionic Interactions -Chelating Groups 15. Chelating Groups 16. Chelating Interactions 17. Solubility 18. Sorbents 19. Protein Binding 20. Assessment SPE Overview SPE Mechanisms Method Development Primary Sample Preparation Techniques Liquid / Liquid Extraction Techniques Approaches to Automation for SPE

9 Infrared Spectroscopy Infrared Spectroscopy Introduction to IR Spectroscopy 1. Electromagnetic Spectrum 2. Electromagnetic Radiation 3. Infrared Regions 4. Molecular Vibrations 5. Calculation of Molecular Vibrations 6. Infrared Active Modes 7. Absorption Considerations 8. The IR Spectrum 9. Infrared Absorption Signals 10. Dispersive IR Instruments 11. FTIR Instruments 12. FTIR Operation 13. Sample Preparation 14. Attenuated Total Reflectance (ATR) 16. Applications of IR Spectroscopy 17. References 18. Assessment Infrared Spectral Interpretation 1. Infrared Spectral Quality 2. Orbital Hybridization 3. Interpretation Concepts 4. IR Frequencies 5. Alkanes 6. Alkenes 7. Alkynes 8. Aromatic Compounds...

10 Basic Lab Skills coming spring 2015 Balance Locationy The location of a balance is important to ensure accuracy when weighing. Manufacturers will have specifications regarding the correct location of a balance within the laboratory. Draughts Balances should be located in a draught free location. This includes draughts from doors, windows, air conditioning, passers-by, and other equipment. Draughts will result in fluctuations in the balance reading. Many analytical balances will have doors to shield the weighing plate from draughts... BASIC LAB SKILLS. Laboratory Safety. Errors. Volumetric Lab Equipment. Balances. Measuring ph. Titrations. UV-visible Spectroscopy 1. Bonding Theory for UV-visible Spectroscopy 2. The Electromagnetic Spectrum 3. Origin of UV-Visible Spectra 4. Transmittance and Absorbance 5. Selection Rules and Intensity 6. UV-visible Chromophores 7. UV-visible Absorption Maxima of Chromophores 8. UV-visible Definitions 9. UV-visible Instrumentation 10. Optical Arrangements of Spectrophotometers 11. UV-Visible Instrument Configuration 12. Key UV-Visible Instrument Parameters

11 Bio Pharmaceutical Analysis coming summer 2015 Protein biopharmaceuticals such as monoclonal antibodies and recombinant proteins are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc. Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge... BIO PHARMACEUTICAL ANALYSIS. Introduction 1. Global Pharmaceutical Market Overview 2. Basics of Protein BioChemistry Amino Acids 3. Basics of Protein BioChemistry Proteins 4. Monoclonal Antibiodies (mab) in Bipharmaceuticals 5. Monoclonal Antibiodies Form and Function 6. Protein Development and Manufacture 7. Biopharmaceuticals Advances in Efficacy and Safety 8. Trastuzumab a model monoclonal antibody (mab) 9. Trastuzumab production and characteristics 10. Trastuzumab common post translational modifications 11. Common mab Post Translation Modifications (PTM s) 12. Analytical Characterisation at the Protein Level 13. Reversed Phase HPLC Analysis of Intact and Reduced Proteins 14. Chromatographic Requirements 15. Chemical Derivatisation

12 Video Training Courses Watch, listen and learn with our new video courses. Each course contains 4 video training sessions, released over 4 weeks, with full tutor support and certification. Fundamentals of HPLC (Dr. Dawn Watson) Fundamentals of GCMS (Tony Taylor) Fundamentals of GC (Tony Taylor & Amy Claydon) Fundamentals of LCMS (Tony Taylor & Amy Claydon) HPLC Method Development (Tony Taylor) GC Method Development (Tony Taylor)

13 Member Overview Top Job Functions Analyst Chemist Lab Manager Mfg/Processing Purchasing QA/QC R&D Teaching Technical Services Toxicology Education PHD: 39% BA/BS/BSC: 56% Years of Experience 10+: 45% 6-10: 25% 1-5: 30%

14 Interactive Learning powered by CHROMacademy contains a vast library of high-quality animated and interactive e-learning topics

15 Search for things s of elearning topics, articles and applications Fix things... interactive tools for chromatographers We developed our Troubleshooters with busy chromatographers in mind. In 3 simple steps you overcome your instrument, separation and quantitation issues. GC Troubleshooter HPLC Troubleshooter

16 HPLC Troubleshooter Select up to 3 Chromatographic Symptoms Positive baseline drift Negative baseline drift Irregular baseline drift Cycling Baseline (Short Term) Cycling Baseline (Long Term) Erratic baseline Regular baseline noise Irregular baseline noise Baseline Spikes Not the Chromatogram I was expecting! a Loss of resolution (some peaks) Loss of resolution (all peaks) No Peaks Additional peaks (ghost peaks) Negative peaks (One or more peaks) Peak broadening (Loss of Efficiency) Normal Peak fronting (one or more peaks) Peak shouldering (one or more peaks) Peak splitting (one or more peaks) Peak tailing (one or more peaks) Rounded peaks Problem Change in selectivity Decreasing retention times Increasing retention times Too much retention 6 Select up to 2 Instrument Symptoms a Low pressure High pressure Continuously decreasing pressure (no sample injection) Continuously decreasing pressure (after sample injection) Continuously increasing pressure (no sample injection) Continuously increasing pressure (after sample injection) Pressure fluctuation Leaks in the solvent reserviour connective tubing Leaks at the degasser / fittings Leaks around the pump head Leaks around the check valves or fittings Leaks in the autosampler or connective tubing Leaks around the sampling valve Leaks at the column fittings Leaks from the detector fiitings Leaks within the detector Poor quantititive reproducibility Poor linearity of response Possible Causes 1. Incorrect / non-optimal mobile phase ph 2. Incorrect column geometry / dimensions 3. Buffer precipitation 4. Incorrect buffer / non-optimal buffer concentration 5. Analyte-stationary phase secondary interaction (e.g. silanol interactions) 6. Incorrect mobile phase 7. Incorrect mobile phase temperature 6more Solution Mobile phase ph affects retention of ionisable analytes and the selectivity of separations in reversed phase HPLC Some methods require a very precise ph in order to remain robust Low ph tends to increase retention of acidic analytes and decrease retention of basic analytes 6more Resources Buffers and ph Meter This video explains how to prepare a buffer and use a ph meter. Buffer preparation This useful tool will help you to prepare the correct buffer for your HPLC analysis How to calibrate ph meter This video describes how to calibrate your ph meter 6more

17 HPLC GC SPE LCMS SPE HPLC GC MS IR BLS BIO Ask The Expert Can t find the answer to your question? Ask our panel of experts with a combined experience of over 100 years in chromatography. 24 hours is all we need to answer your question. Most often, we ll get something to you before then. Complex scenarios welcome! Problem solved!

18 Socialize powered by The Forum is your meeting place to learn and communicate with others interested in analytical science

19 powered by Monthly Activities Webcasts Live webcasts featuring industry experts, LCGC editors and CHROMacademy technical staff - archive available 24/7 Tutorials Comprehensive study guides with interactive content that dives deeper into the coresponding webcast Featured Content New content focusing on a technique or instrument Newsletter Tips, tricks and practical no nonsense solutions from chromatography experts each month Daily News RSS feeds from around the world Application Notes Thousands of application notes from instrument vendors

20 The Monthly Essential Guides. Each topic is covered with a 1hr webcast and comprehensive tutorial.

21 CHROMacademy newsletter Tips, tricks and practical no nonsense solutions from chromatography experts each month

22 Certified courses with tutor support. HPLC Operator Course HPLC Method Developer Course GC Operator Course GC Method Developer Course Each course covers all the key topics and is created by our experts in analytical chemistry. You can take a course at your own pace without a tutor if you wish, or you can opt in to have full tutor support and agree a timescale to complete the course on.

23 Reporting Tools. powered by With a multi user account you can set learning goals & track user progress

24 Saving Time & Money powered by Scientist A needed to understand why her chromatogram contained baseline spikes combined with a loss of sensitivity. She turns to CHROMacademy s HPLC Troubleshooter and in 2.3 seconds, CHROMacademy tells her there are 26 possible causes for this combination of symptoms. Each possible cause is ranked using our unique expert system and includes a comprehensive explanation and suggested fixes backed by 100 s of resources from LCGC magazine, CHROMacademy and instrument vendors. Without a CHROMacademy Premier Membership: Called her manufactures representative. 36 hours before someone called back Lost time of instrument use: 40 hours Lost $ = $2,000 (@$95K/annum) Contacted her Lab Manager 2 hours lost before Lab Manager could be located

25 Saving Time & Money powered by Scientist B needed to develop a sensitive GC-MS method for a chlorinated pesticide derivative present in potable water samples at very low levels. The derivative was new and therefore no applications existed in manufacturers literature. Using a CHROMacademy Webcast and Tutorial he was able to develop a GC method from a generic screening method and realized that Chemical Ionization was the route to attain the required sensitivity. From the Sample Preparation Channel he noted that solid phase extraction could be used to selectively extract the target analyte and again was able to develop a method from a suggested generic starting procedure. He used the ask the expert function to enquire of the CHROMacademy academic team how to create a sensitive MS acquisition method whilst checking for the presence of co-eluting interferences. Method was developed and was ready for validation within 1 week. Without a CHROMacademy Premier Membership: He wished that the department GC-MS expert hadn t been laid off.. Method development now begins using guesstimates

26 Benefits of a Group Premier Membership» Multiple Use $ Discount» Decrease in Travel Time and Money Used For Vendor Training» Satisfies Legislative and/or Regulatory Requirements for Training Within Your Industry and Laboratory» Easily Bolts on to Existing Learning Management System (SCORM Compliant)» Access to the Web s Largest Repository of Theory and Practical Information in Chromatography & Mass Spec.» Produces Common Lexicon» Learning Manager s Reporting Tools» Offers Centralized Billing» Recognized Leader in LCGC Magazine For Quick Buy-In From Staff» and Other Messaging to Reinforce Staff Engagement» Assessments and Tutorials With Printable Completion Certificates For Compliance» Trusted Vendor» Completely Web-Based (No IT Involvement Needed)» Certification Programs 2-5 Premier Membership $ Group Membership Available. Please contact your representative

27 Options Available Microsite Your Landing Page Completely build from top to bottom your very own learning site powered by CHROMacademy Your Logo and 1-2 Channels Customized Assessments Based on content agreed upon Certificate of Completion Certification Programs Offers your learners a goal to strive towards LMS - Connect Engagement Package Our Learning Management System is S.C.O.R.M. compliant and will connect to your system E-newsletter program derived from your content and ours Video Training Courses Live at Your Place Watch, listen and learn with our new video courses. Live in-person training including take home courseware, testing and certificates

28 Premier Membership Sales Single user - $ l l Group discounts available Contact sales@support.chromacademy.com Request a quote: Click here for our website submission form Alternatively you can contact our sales team directly USA & Rest of the World Sales Glen Murry phone: gmurry@advanstar.com United Kingdom Sales Crawford Scientific phone: +44 (0) enquiries@crawfordscientific.com Advertising/Sponsorship Mike Tessalone phone mtessalone@advanstar.com Technical Solutions sales@support.chromacademy.com