Training Courses 2014 HPLC GC SPE

Transcription

1 Training Courses 2014 HPLC GC SPE

2 min 8 2 Courses & Presenters HPLC How to Run HPLC Methods Price: VAT Code: SS Page: 4 How to Develop HPLC Methods Price: VAT Code: SS Page: 5 How to Develop HPLC Methods for challenging separations Price: VAT Code: SS Page: 6 How to Troubleshoot HPLC Price: VAT Code: SS Page: 8 Meet the presenter: Oona McPolin Oona has considerable experience and is fully qualified in the areas of both pharmaceutical analysis and training practice. She has worked as an analytical chemist in the pharmaceutical industry for over 10 years on a range of drug development projects and has been responsible for many pharmaceutical analysis training programmes during this time. Her area of expertise is in the technique of HPLC with particular interest in strategies for method development. Oona is the author of two books, An Introduction to HPLC for Pharmaceutical Analysis and Validation of Analytical Methods for Pharmaceutical Analysis. Oona has obtained the industry standard qualification for training, the Certificate in Training Practice, awarded by the Chartered Institute of Personnel and Development (CIPD). Qualifications: 1993 BSc Hons Chemistry, Queen s University of Belfast 1994 MSc Applied Environmental Sciences, Queen s University of Belfast 2008 Certificate in Training Practice, Chartered Institute of Personnel and Development (CIPD). Received a High Achiever s Award from the CIPD NI branch Affiliations: Member of the Royal Society of Chemistry (MRSC) Chartered Chemist (CChem) Chartered Scientist (CSci) Associate member of the Chartered Institute of Personnel and Development (CIPD)

3 min 8 3 GC The Gas Chromatography Seminar Code: SS Page: 9 Meet the presenter: Melanie Emmanuel Melanie Emmanuel graduated from University College London with a BSc in Pharmacology. She joined in 2004 as an Account Manager focusing on our Gas Chromatography product line. After three years of working across all industries Melanie was promoted in 2008 to Key Accounts Manager and Group Leader. As a Key Account Manager she has become an industry expert in both the Toxicology and Environmental markets. Her role allows her to develop customer methods in our lab, give technical presentations both at conferences and at customer facilities, and visit hundreds of labs across the USA. She also assists with new product development to ensure that our gas chromatography line continues to strengthen and grow. It is because of her knowledge, expertise and in credible work ethic she was selected to lead training for our Singaporean distributor. Melanie also ran this successful series of GC seminars across the Nordic region and the UK in spe The SPE Method Developers Seminar Code: SS Page: 10 Meet the presenter: Dr. Raymond Wong Dr. Raymond Wong s background is in Synthetic organic chemistry. He completed his Ph.D.in synthesis of pyrrolidine based oligonucleotides, using solid phase peptide protocols. Additionally, his Post doctoral research is in dynamic combinatorial libraries using poly ethylene glycol resin beads. Following his role as a developmental chemist purifying active pharmaceutical compounds up to multi-kilo scale, Raymond joined. Solid Phase Extraction During his 4 years with, Raymond has become the SPE Specialist for customers in the UK and Ireland. He has considerable experience and is fully qualified in the areas of pharmaceutical analysis, specializing in sample preparation techniques such as Solid Phase Extraction (SPE). His knowledge reaches from drugs of abuse in toxicology, to polyaromatic hydrocarbons sample preparation in the environmental sector. A key responsibility as SPE Specialist at, involves Raymond delivering dynamic, customized training courses. This has included both theoretical and practical learning programs at customer facilities. Every course includes: First class training Training materials booklet Lunch and refreshments Post-training online assessment, with certificate of completion Training at your site? Ask your technical consultant about training for you and your colleagues, at your site. Save time / Save costs with first class training for your whole team.

4 4 1] How to Run HPLC Methods Course no. SS Course summary: Learn how to set up and run HPLC analysis with full understanding of all the method parameters such as the column, the mobile phase, the instrumentation, and sample preparation, and how to interpret and quantify the results of the analysis. This course is ideal for those who are new to HPLC. Course outline: HPLC method parameters Columns and stationary phase Mobile phase Instrumentation Preparation of test solutions Directions for analysis Setting up HPLC systems for analysis Interpreting results from HPLC analysis Integration System suitability Quantification techniques Practical skills acquired: This course will enable you to implement HPLC analytical methods by transferring the parameters from the method to your HPLC system. In addition you will be able to: 1. Understand what is meant by all the parameters in an HPLC analytical method. 2. Follow an HPLC analytical method to set up an HPLC system for analysis. 3. Run an HPLC analytical method and acquire chromatographic results. 4. Interpret chromatograms obtained from HPLC analysis. 5. Calculate analytical results for HPLC analysis. Dates: London Warrington

5 5 2] How to develop HPLC Methods Course no. SS Course summary: Learn how to select appropriate method conditions and perform suitable investigative experiments to obtain a set of method parameters which enables the desired separation for mixtures of analytes. This course is ideal for those who have experience running HPLC methods and now want to learn how to develop new methods. Course outline: Developing an HPLC method using a 5-step strategy: 1. Setting suitable objectives for method development 2. Assessing all available information 3. Selecting suitable samples 4. Performing scouting experiments to select suitable initial conditions 5. Optimising the method to define method parameters which achieve the desired separation This course focuses on reversed phase mode separations. Practical skills acquired: This course will enable you to take a strategic approach to developing HPLC methods with an understanding of the factors which can be adjusted to manipulate the retention time of analytes. In addition you will be able to: 1. Define the objectives for the development of an HPLC analytical method. 2. Effectively assess all the available relevant information relating to the desired method, e.g. pka of the analyte. 3. Select and prepare a suitable sample or samples to be used for the method development. 4. Select suitable scouting conditions to find a suitable column and mobile phase system. 5. Optimise the chromatographic conditions to result in the best possible separation. Dates: London Warrington HPLC

6 6 3] How to Develop HPLC Methods for Challenging Separations Course no. SS Course summary: Learn how to implement strategies to achieve satisfactory separation for complex samples and use computer modelling to develop robust and fit for purpose HPLC methods. This one day course is ideal for those who have experience of developing HPLC methods but want to increase their knowledge to deal with more challenging separations. Although designed as a follow-up to the course How to Develop HPLC Methods, it is not a prerequisite. This course is suitable for learners who feel confident that they are familiar with all the content detailed for the course How to Develop HPLC Methods. Course outline: Implementing a 5-step strategy for method development of complex samples Review of a 5-step strategy for HPLC method development with particular attention to reasons why some separations are challenging : 1: Setting suitable objectives for method development 2: Assessing all available information 3: Selecting suitable samples 4: Performing scouting experiments to select suitable initial conditions 5: Optimising the method to define method parameters which achieve the desired separation Separation Theory Method development objectives: Strategies for maximising resolution in HPLC using retention factor, k, selectivity, α, and efficiency, N.

7 7 Case Study Using Computer Modelling A case study is used to demonstrate how a computer model may be used to find the best conditions for the separation. Strategy 1: Sample Preparation A general approach to sample preparation is provided with considerations regarding the most suitable technique. Strategy 2: Detection Techniques How to choose a suitable detector when analytes are not UV active: the different types of detectors used for HPLC, derivatisation to improve detectability. Strategy 3: Retaining Very Polar Analytes Techniques which enable analysis of very polar molecules by reversed phase HPLC: High ph, aqua columns, mixed mode columns, HILIC, Ion-pairing chromatography. Strategy 4: Optimising Gradient Methods Understanding gradient analysis; using multi-segment gradients for complex mixtures of analytes. Strategy 5: Selecting Columns Selecting columns which give different selectivity tools for column comparison. Strategy 6: Scouting/Screening Experiments Setting up multiple scouting experiments for complex sample mixtures. Practical skills acquired: This course will enable you to find solutions for difficult HPLC separations. In addition you will be able to: 1. Understand why some separations can be challenging. 2. Identify potential problem separations. Apply strategies to achieve satisfactory separations for complex samples with respect to: 1. Sample preparation 2. Detection methods 3. Retaining very polar analytes 4. Optimising gradient methods 5. Selecting columns 6. Setting up scouting/screening experiments Dates: Edinburgh Warrington London HPLC

8 8 4] How to troubleshoot HPLC Course no. SS Course summary: Learn how to find solutions for problems encountered when running HPLC analysis by diagnosing symptoms and implementing appropriate preventative measures. This course is ideal for those who have experience using HPLC and now want to develop their skills further. Course outline: Overview of the HPLC and how it works: Mobile phase, pumps, injectors, columns, detectors and connections Common problems and preventative measures Problem solving strategy: Assessing the symptoms Making diagnosis Finding the appropriate solution Practical skills acquired: This course will enable you to go back to your lab with a full understanding of why problems may arise with your HPLC system and give you the skills and knowledge to both prevent and resolve those problems. In addition you will be able to: 1. Understand how HPLC works and the role of each component in an HPLC system. 2. Understand how problems can arise in the individual components of an HPLC system. 3. Implement measures which prevent problems occurring. 4. Use a systematic problem-solving approach to HPLC troubleshooting. 5. Diagnose and resolve problems associated with HPLC. Dates: Warrington Edinburgh London

9 9 5] Gas Chromatography Seminar Course no. SS Course Summary Join us for this 1 day training course, which provides a comprehensive working knowledge of GC methods, systems and consumables. Course Outline Areas of GC covered: The Basics of Gas Chromatography Method Development and Optimisation GC Troubleshooting Inlets, Detectors, Columns and Accessories This course is ideal for: Scientists new to Gas Chromatography Chromatographers with experience of running GC methods, but looking to develop new methods Gas Chromatographers wishing to refresh their knowledge of GC and to learn new troubleshooting tips Dates: Warrington London Newcastle gc

10 10 6] SPE Method Developers Seminar Course no. SS Course summary: An in depth course that will demonstrate how to instantly improve GC and HPLC analysis through the use of Solid Phase Extraction (SPE). Course Outline: SPE Extraction Protocol SPE Sample Pre-treatment SPE Column Conditioning SPE Column Equilibration Sample Loading Column Washing SPE Separation Mechanisms Reversed-phase SPE Normal phase SPE Cation-Exchange SPE Anion-Exchange SPE Mixed-Mode SPE Molecular Properties Hydrophobic or Non-Polar Groups Polar Groups Ionic Groups Chelating Groups SPE Method Development Analyte Assessment Mechanism Selection Sorbent Screening Procedure Optimization Analyte Elution This course is ideal for: Dates: Any analyst who wishes to improve their GC and HPLC analysis. Previous knowledge: An understanding of the principles of chromatrography would be advantageous. Previous knowledge of SPE is not required Edinburgh Belfast London Each course includes full course instructions, simple SPE protocol and a starter pack of SPE tubes, to get started once back in the lab Warrington

11 Registration Form Please enter details for all courses, including delegate contact details. Then scan and to or Fax to +44 (0) Course Registration Course # Location Date Delegate Name Delegate Invoice Information Company Name: Contact Name: Contact Tel: Contact Address 1: Address 2: Town / City: Post code: Purchase Order no: If you wish to pay via credit card, please check this box. Card: Register online at: Call: (UK) (Eire) Terms and Conditions Subject to Standard Terms & Conditions, which may be viewed at , Inc. All rights reserved. 11

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