CHROMOUS BIOTECH. Research Services. Spectrum of Innovation. An ISO Certified Company

Transcription

1 Spectrum of Innovation Research Services ChromoVISION Services & Products Research Instruments Plastic Consumables An ISO Certified Company

2 CHROMOUS undertakes outsourced projects in all related domains of r-dna technology, immunology, epigenetics, glycobiology, protein expression / purification and plant molecular biology services. We work closely with the esteemed scientists to ensure time-bound, positive outcome of all customized projects accepted. The company has successfully completed several projects around the world. The Research services from Chromous Biotech include DNA Sequencing (Sanger) Genome / Transcriptome / Exome / Targeted Sequencing Gene Cloning & Manipulation Protein Expression & Analysis Metagenomic Services Genotyping Services Plant Molecular Biology Services Real-Time PCR Services Epigenetics services Glycobiology services Cell Biology, Cell Cultures services & Immunolgy services Di-Deoxy Sequencing solutions - Services: Sequencing of unpurified clones / plasmids / PCR products Sequencing of purified clones / plasmid DNA/ PCR products Primer-walking Sequencing native bacterial plasmids SNP detection and data analysis Methylation analysis by bisulfite sequencing SSCP analysis HLAClass I, Class II Typing PCR, Cloning & Sequencing DNA oligonucleotide synthesis service

3 Genome / Transcriptome / Exome / Targeted Sequencing Microarray Services (Agilent and Affimatrix platforms) Sample processing, labeling, hybridization, scanning and data analysis Genome Sequencing Services (on Roche GS FLX) Paired-end sequencing; Microsatellite discovery; Transcriptome sequencing and Metagenome Sequencing etc., Metagenome sequencing Reference based mapping / de novo assembly & functional annotation Phylogenetic / taxonomical analysis, functional category / diversity identification Rank abundance, rarefaction curve, gene / pathway identification & analysis Genome Sequencing Services (on Illumina HiSeq 2000) RAD markers; Exome sequencing; Small RNA analysis; Whole transcriptome analysis; Single end sequencing; Paired end sequencing; Whole genome resequencing and Mitochondrial DNA Sequencing Bioinformatics Services Small RNA analysis; Microarray data analysis; Physical, genetic & QTL mapping; Assembly and Annotation of prokaryotic and Eukaryotic Genome; Genome mapping and SNP discovery; Transcriptome analysis (De novo / reference based) & Metagenomics analysis Gene Cloning & Manipulation Full-Length cdna Cloning Services Development and screening of cdna / EST Library / SSH Library Gene Engineering Services Synthetic Gene Synthesis Site-Directed mutagenesis; Random mutagenesis of a Cloned Gene; Random mutation of gene(s) on host Genome Protein Expression & Analysis Genes are expressed in bacterial, Pichia and mammalian systems and are purified Protein re-folding to biologically active form Blotting Services : Western, Southern, Northern

4 Metagenomic Services Bacterial identification: Around 1,500 bp of 16S rdna sequence based molecular identity, Fungal Identification Service: ITS region based molecular identity established Fish Identification Service: COI region sequence-based molecular identity Bacterial population analysis: Analysis of Number of different microbes Bacterial population analysis: Identification of all bacteria in a mixed population Genotyping Services Development of Microsatellite library Genotyping by RAPD, AFLP and SSR/ STR analysis; SNP detection Authentication of samples by genotype-based barcoding Development of ARMS and SCAR markers Fragment analysis by GeneMapper/ GeneScan Plant Molecular Biology Services Plant Identification, Hybrid Seed Purity Tested Transgenic Event Characterization for transgenic plants Real-Time PCR Services Copy No. detection, Gene expression profiling and PCR-arrays Gene Knock-out / Knock-in and Gene Silencing Services Knock-out / Knock-in of a gene or set of genes Development of recombinant microbes by introduction of foreign genes sirna, shrna design and synthesis: Designed and synthesized for different systems Drosophila sirna: Available for silencing all drosophila genes individually

5 Gene Silencing by Lentivirus based protocols shrna lentiviral services; Over-Expression lentiviral services mirna expression and anti-mirna lentivirus services Virus Production (packaging) service Proteomic Services Standard peptide, Modified, Labeled Peptide Synthesis Disulfide mapping (plus/minus reduction & alkylation) GlycoPeptide mapping (Tryptic Peptide mapping plus/minus PNGase F) Heterogeneity by IEF MALDI-TOF MS of Intact Protein / peptide Measurement of Protein Deamidation, Extinction Coefficient N-terminal sequencing (per cycle) Size-Exclusion, Anion-Exchange, Reverse-Phase Chromatography Oligosaccharide Profiling of glycopepetides; Quantitative Amino Acid Analysis (AAA) Epigenetic Services For studying DNA Methylation / demethylation and other modifications Bisulfite DNA Modification DNA Methylation Quantification Methylated DNA Amplification, Immunoprecipitation DNA Methyltransferase & Demethylase Assays Chromatin & Transcription General Chromatin Immunoprecipitation In Vitro Protein-DNA Interaction Methyl-Histone Chromatin IP, Acetyl-Histone Chromatin IP Methyl-DNA Binding Protein ChIP Histone Methylation, Acetylation & Deacetylation studies: HAT, HDAC and Acetylation assays

6 Cell Biology & Cell Culture Services Glycobiology Services Immunology Services Antibody gene cloning Customized antibody pair development for sandwich ELISA Expression of biologically active soluble protein Western Blotting and detection of antigens Enzyme Assays: Cell Viability, Cytotoxicity, Apoptosis, Cell based potency tests for antimicrobial / antifungal / anticancer agents, Quantification of "free radical" generated In vitro, 8-Oxo-G detection and quantification, Comet Assay and Target Screening Assay Development of Lateral Flow Assay based detection system CARBOHYDRATE ANALYSIS Gal Alpha Gal analysis: Measures galactose linked α1-3 to another galactose molecule Glycoprotein analysis: Studies of oligosaccharide structures on glycoproteins by HPLC & MALDI-TOF Glycosidase sequencing: Selective release of monosaccharides from oligosaccharide based on linkages Mannose 6-Phosphate analysis: Measures (Man 6-P/M6P) released from glycoproteins by acid hydrolysis Neutral monosaccharide composition: Samples are acid hydrolyzed and analyzed by HPAEC N-linked and O-linked oligosaccharides profiling: Generated in HPLC / MALDI- TOF MS / HPAEC-PAD Polysaccharide analysis: by HPAEC with PAD and MALDI-TOF MS for identification and analysis Sialic acid analysis: Samples are hydrolyzed and analyzed by HPAEC using electrochemical detection. Monoclonal and Polyclonal Antibody development, purification and Labeling Service: with HRP / ALS / FITC / Gold Development of Stable cell lines expressing a fluorescent protein: Luciferase stable cell line with different selection marker: LoxP-GFP/RFP color switch cell lines TetR (tetracycline Regulator) stable cells with different marker CRE stable cell line with different selection marker

7 CHROMOUS PRODUCTS & REAGENTS Molecular Biology Products Research Kits DNA Ladders and Markers Epigenetic products RNAi products Plasmids and Genomic DNA Restriction and Modifying Enzymes PCR Enzymes and kits RT-PCR Enzymes and kits DNA/ RNA Purification Kits Q-PCR Kits PCR Kits Gene Cloning Kits Immunology Kits Protein related Kits Immunology Reagents Teaching Kits Whole Serum and Affinity Purified Serum ALP / HRP / FITC / BIOTIN conjugates Affinity Purified Immuno- Globulins Biosolutions / Powders Agarose, X-Gal, IPTG, Lysozyme, Alkaline Phophatase, Streptavidin, Protein A resin, CM-Cellulose, DEAE- Cellulose, Nickel-agarose, Phospho-Cellulose. Basic and Advanced Molecular Biology related kits Microbiology related Kits Biochemistry related Kits Immunology related Kits Medical Biochemistry related Kits Contact: sales@chromous.com or call: or visit:

8 CHROMOUS RESEARCH INSTRUMENTS Agarose and Polyacrylamide Gel Apparatus: mini, midi and maxi Blotting Apparatus: Western and Southern Power Supplies: up to 3,000 V, multiple gel connects Centrifuges: bench-top, 6,000 rpm to 17,000 rpm Incubator Shaker, BOD incubator: Cooling and Heating Thermal cycler: 24-well, 48-well and 96-well UV / VIS spectrophotometer: Dual channel, highly sensitive ph meter: Digital Analytical balances: sensitive up to 0.1 mg Laminar air flow chamber: BioSafety Level 1 and 2 Biosafety cabinets: BioSafety Level 1and 2 Deep freezer: -20 C and -80 C Autoclave: (Vertical) ELISA Reader: Fixed and variable wave-length models Microwave Oven Gel Documentation System Plastic Consumables: PCR vials: Transparent, safe-lock Microfuge tubes: 0.5 ml, 1.5 ml and 2.0 ml, Transparent Pipette tips: 10 μl, 200 μl and 1 ml, transparent and colored Beakers / Bottles / Flasks Tissue culture plastics: Culture plates, Culture Flasks, Pipettes, g-irradiated PCR plate: 96-well, with or without skirting 96-well sequencing plate: ABI compatible ELISA plate Petriplates: Bacteriological Centrifuge tubes: 15 ml, 50 ml, conical and round bottom Corporate Office: Chromous Biotech Pvt. Ltd. # 842, II/ III Floor, Sahakarabhavan, 'A' Block, Sahakaranagar, Bangalore Phone: Office: / / Contact: sales@chromous.com or call: or visit: