European Subcommittee on Antifungal Susceptibility Testing (EUCAST AFST)
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1 Europen Suommittee on Antifungl Suseptiility Testing (EUCAST AFST) Routine nd extended internl qulity ontrol for ntifungl suseptiility s reommended y EUCAST Version 2.0, vlid from 22 nd of June, 2018 This doument should e ited s "The Europen Committee on Antimiroil Suseptiility Testing. Routine nd extended internl qulity ontrol for ntifungl suseptiility testing s reommended y EUCAST. Version 2.0, Generl Pge Notes 2 Routine qulity ontrol Pge Cndid krusei ATCC Cndid prpsilosis ATCC Cndid lins CNM-CL F Cndid krusei CNM-CL Aspergillus fumigtus ATCC Aspergillus flvus ATCC Aspergillus flvus CNM-CM A. fumigtus SSI A. fumigtus SSI Pge 1 of 11
2 Notes 1. In EUCAST qulity ontrol (QC) tles, oth rnges nd trgets re listed. Repet testing of EUCAST qulity ontrol strins should yield individul MIC vlues rndomly distriuted within the reommended rnges. If the numer of tests is 10, the mode MIC should e the trget vlue. 2. EUCAST qulity ontrol strins for routine QC re used to monitor test performne. Control tests should e set up nd heked dily, t lest for ntifungl gents whih re prt of routine pnels. 3. For informtion out the referene methods for suseptiility testing of yest nd moulds see Methods in ntifungl suseptiility testing of yests nd Methods in ntifungl suseptiility testing of moulds, respetively. Pge 2 of 11
3 Cndid krusei ATCC 6258 Antifungl Agent Trget MIC (mg/l) Rnge Amphoteriin B Anidulfungin Cspofungin NA NA Fluonzole Fluytosine Isvuonzole Itronzole Mifungin Vorionzole Posonzole ATCC: Amerin Type Culture Colletion. Cndid QC strins requires reding in spetrophotometer fter one dy of inution. Not ville Pge 3 of 11
4 Cndid prpsilosis ATCC Antifungl Agent Trget MIC (mg/l) Rnge Amphoteriin B Anidulfungin Cspofungin NA NA Fluonzole Fluytosine Isvuonzole Itronzole Mifungin Vorionzole Posonzole ATCC: Amerin Type Culture Colletion. Cndid QC strins requires reding in spetrophotometer fter one dy of inution. Not ville Pge 4 of 11
5 Cndid lins CNM-CL F8555 Antifungl Agent Trget MIC (mg/l) Rnge Amphoteriin B Anidulfungin NA NA Cspofungin NA NA Fluonzole Fluytosine Isvuonzole NA NA Itronzole Mifungin NA NA Vorionzole Posonzole CNM-CL: Yest olletion of the Spnish Ntionl Centre of Miroiology. Aville from the EUCAST Development Lortory (EDL) for fungi: Cndid QC strins requires reding in spetrophotometer fter one dy of inution. Not ville Pge 5 of 11
6 Cndid krusei CNM-CL-3403 Antifungl Agent Trget MIC (mg/l) Rnge Amphoteriin B Anidulfungin NA NA Cspofungin NA NA Fluonzole Fluytosine Isvuonzole NA NA Itronzole Mifungin NA NA Vorionzole Posonzole CNM-CL: Yest olletion of the Spnish Ntionl Centre of Miroiology. Aville from the EUCAST Development Lortory (EDL) for fungi: Cndid QC strins requires reding in spetrophotometer fter one dy of inution. Not ville Pge 6 of 11
7 Aspergillus fumigtus ATCC Antifungl Agent Trget MIC (mg/l) Rnge Amphoteriin B Cspofungin NA NA Isvuonzole NA NA Itronzole Mifungin NA NA Vorionzole Posonzole ATCC: Amerin Type Culture Colletion. Aspergillus QC strins should e red visully t no growth inhiition endpoint fter 2 dys of inution. Not ville Pge 7 of 11
8 Aspergillus flvus ATCC Antifungl Agent Trget MIC (mg/l) Rnge Amphoteriin B Cspofungin NA NA Isvuonzole NA NA Itronzole Mifungin NA NA Vorionzole Posonzole ATCC: Amerin Type Culture Colletion. Aspergillus QC strins should e red visully t no growth inhiition endpoint fter 2 dys of inution. Not ville Pge 8 of 11
9 Aspergillus flvus CNM-CM-1813 Antifungl Agent Trget MIC (mg/l) Rnge Amphoteriin B Cspofungin NA NA Isvuonzole NA NA Itronzole Mifungin NA NA Vorionzole Posonzole CNM-CM: Spnish Ntionl Centre for Miroiology, Filmentous Fungi Culture Colletion. Aville from the EUCAST Development Lortory (EDL) for fungi: Aspergillus QC strins should e red visully t no growth inhiition endpoint fter 2 dys of inution. Not ville Pge 9 of 11
10 A. fumigtus SSI-4524 QC strin hrouring TR34/L98H sustitution for qulity ontrol of zole-ontining gr pltes. Eh well of the plte ontins the following zoles: well 1 (itronzole 4 mg/l), well 2 (vorionzole 2 mg/l), well 3 (posonzole 0.5 mg/l), nd well 4 (drug free ontrol well). Expeted growth sores fter 48 h Sore definition: 0: no visile growth; 0.5: 1-5 tiny olonies; 1: wek/miniml growth (either s >5 tiny olonies or onfluent wek growth where the gr hs een inoulted (overing hlf the well)); 2: lerly visile growth with hyphl extension ut not overing the entire well (nd less thn the ontrol well); 3: un-inhiited prominent growth overing the mjority of the well nd similr to tht in the ontrol well. SSI: Sttens Serum Institut, Copenhgen, Denmrk Aville from the EUCAST Development Lortory (EDL) for fungi: Pge 10 of 11
11 A. fumigtus SSI-5586 QC strin hrouring G54W sustitution for qulity ontrol of zole-ontining gr pltes. Eh well of the plte ontins the following zoles: well 1 (itronzole 4 mg/l), well 2 (vorionzole 2 mg/l), well 3 (posonzole 0.5 mg/l), nd well 4 (drug free ontrol well). Expeted growth pttern fter 48 h: Sore definition: 0: no visile growth; 0.5: 1-5 tiny olonies; 1: wek/miniml growth (either s >5 tiny olonies or onfluent wek growth where the gr hs een inoulted (overing hlf the well)); 2: lerly visile growth with hyphl extension ut not overing the entire well (nd less thn the ontrol well); 3: un-inhiited prominent growth overing the mjority of the well nd similr to tht in the ontrol well. SSI: Sttens Serum Institut, Copenhgen, Denmrk Aville from the EUCAST Development Lortory (EDL) for fungi: Pge 11 of 11
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