GenScript Magnetic Beads

Transcription

1 Products GenScript Magnetic Beads Advance Your Proteomics Research The Biology CRO

2 Magnetic Beads Separation GenScript Magnetic Beads Advance Your Proteomics Research Quick, easy and convenient magnetic separation procedures Extremely gentle sample handling with no loss of your target High binding capacity and reproducible results Ideal for immunoprecipitation and micro-scale protein and antibody purification Over these years, a wide range of applications in Microbiology, Immunology and Molecular Biology has been proving magnetic separation to be the increasingly powerful technology in life science. GenScript has been continuously developing high quality magnetic products to meet researchers needs in proteomics research and other fields. A comprehensive line of GenScript MagBeads is available for you to achieve rapid and efficient bio-magnetic separation and ensure reliable and reproducible results (Table 1). Table 1 Selection Guide of GenScript Magnetic Beads Application Product Binding capacity Size Cat. No. Protein A MagBeads > 10 mg porcine IgG/ml settled 4 ml L00273 Immunoprecipitation and micro-scale antibody purification Protein G MagBeads > 10 mg goat IgG/ml settled L00274 Protein A/G MagBeads > 10 mg goat or porcine IgG/ml settled L00277 Micro-scale purification of His-tagged proteins Ni-Charged MagBeads 5-20 mg 6 His-tagged protein (27kD)/ml settled L00295 Micro-scale purification of GST fusion proteins Glutathione MagBeads 5-10 mg GST (26kD) /ml settled L00327 of His-tagged proteins Mouse Anti-His mab MagBeads 300 μg 6 His-tagged protein (27kDa)/ml settled L00275 of GST fusion proteins Mouse Anti-GST mab MagBeads 300 μg GST (26kDa) /ml settled L00336 of Trx fusion proteins Mouse Anti-Trx mab MagBeads 300 μg Trx (17kDa)/ml settled L00337 of rabbit IgGs Goat Anti-Rabbit IgG MagBeads 300 μg rabbit IgG/ml settled L00328 of goat IgGs Donkey Anti-Goat IgG MagBeads 300 μg goat IgG/ml settled L00332

3 Protein A, G and A/G MagBeads GenScript Protein A, G and A/G MagBeads are developed for quick, convenient and reproducible immunoprecipitation (IP) and small scale purification of immunoglobulins (Igs). By covalently pre-coupling recombinant protein A, G and A/G to the surfaces of superparamagnetic, these MagBeads provide users simple, easy and time-saving procedures for immunomagnetic separation. Fast, easy and gentle magnetic separation By applying magnetic separation technology, the Protein A, G and A/G MagBeads supply the user fast, easy and efficient IP/isolation procedures. The additional device you need is just a magnetic separation rack (Figure 1). All the steps take place in a single tube. No columns, centrifugation and pre-treatment are needed. Extremely gentle magnetic-handling ensures no loss of your target IgG or protein and keeps them intact during whole procedures. Figure 1. GenScript Multifunctional Magnetic Separation Rack Recombinant protein A, G and A/G ensure efficient IP and Ig purification Due to the elimination of non-igg binding domains, the recombinant protein A, G and A/G both display high affinity for Igs from many species including human, rabbit and mouse etc. This permits the efficient purification of Igs from cell culture supernatants, serum and ascites or other starting samples. The specific binding strength will depend on the species and Ig subclass. In addition, Protein A, G and A/G MagBeads can be used to immunoprecipitate target proteins from crude samples using selected primary antibody. To prevent co-elution of the antibody, it is necessary to chemically cross-link the antibody to protein A, G and A/G coated prior to the immunoprecipitation steps (Figure 2). antibody (Cross-linking optional) Isolate pure antibodies GenScript Protein A, G or A/G MagBeads target protein Add 1xSDS loading buffer and heat to 100 o C Denaturing elution for isolation of denatured protein (No cross-linking) Mild elution for isolation of native protein (Ab cross-linked to protein A, G or A/G ) Figure 2. How GenScript Protein A, G or A/G MagBeads Work

4 Ni-Charged and Glutathione MagBeads Polyhistidine and glutathione S-transferase (GST) are common protein/peptide tags added to the end of a recombinant protein to aid in their purification or detection. GenScript Ni-Charged MagBeads and Glutathione MagBeads provide simple, rapid and reliable methods for micro-scale purification of polyhistidine and GST tagged fusion proteins from crude cell lysate prepared from bacteria, yeast or mammalian cell culture. All the protocols are easily performed by applying the magnetic separation technology. Ni-Charged MagBeads for micro-scale purification of polyhistidine tagged proteins The Ni-Charged MagBeads are average 40μm in size, superparamagnetic with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with Ni 2+ and ready-to-use for quick and micro-scale purification of polyhistidine-tagged proteins under native or denaturing conditions. The Ni-Charged MagBeads show ultra high selectivity for poly-histidine tags, resulting in extremely low background levels. The MagBeads offer exceptionally fast binding kinetics and high yields per microliter of settled. The polyhistidine-tagged protein can be easily purified from samples in less than 1 hour (Figure 3). Sample with target protein Add GenScript Ni-Charged or Glutathione MagBeads Incubate 30 minutes to 1 hour Glutathione MagBeads for micro-scale purification of GST fusion proteins The Glutathione MagBeads provide a fast, easy and efficient microscale purification of glutathione-s-transferase (GST) fusion proteins from a bacteria, yeast or mammalian crude cell lysate. The glutathione is immobilized on surfaces of superparamagnetic mainly through its central carbon for efficient GST binding. The use of non-magnetic GST purification matrices for small-scale batch purification can be time-consuming. The Glutathione MagBeads provide one-step purification, eliminating tedious centrifugation steps, the need for multiple tubes and minimizing sample loss (Figure 3). Separate pure target protein Figure 3. How GenScript Ni-Charged or Glutathione MagBeads Work

5 Magnetic Beads Separation Epitope Tag Antibody Coated MagBeads GenScript Epitope Tag Ab Coated MagBeads GenScript epitope tag antibodies pre-coated MagBeads (e.g. Mouse Anti-His mab MagBeads, Mouse Anti-GST mab MagBeads and Mouse Anti-Trx mab MagBeads) are designed for immunoprecipitation and quick isolation of His-, GST- or Trx-tagged fusion proteins. The epitope tag antibodies display both strong specificity and high affinity for the protein/peptide tags, enabling the MagBeads to quickly and efficiently capture the target tagged proteins from crude cell lysate of bacterial, yeast and mammal cell culture. By using a magnetic separation rack, the captured proteins can be easily eluted off the with very low background. Or instead, the protein bound MagBeads can also be boiled and then directly applied to SDS-PAGE analysis followed by Western Blotting (Figure 4). target protein Isolate pure target protein Figure 4. How GenScript Epitope Tag Antibody Coated MagBeads Work. Secondary Antibody Coated MagBeads GenScript Secondary Antibody Coated MagBeads (e.g. Goat Anti-Rabbit IgG MagBeads and Donkey Anti-Goat IgG MagBeads) are pre-coupled with purified secondary antibodies and display strong specificity and high affinity to corresponding IgGs, allowing for maximum flexibility of your protein and antibody purification. These MagBeads can be easily used for immunoprecipitation and quick, small scale purification of IgGs. Additionally a specific primary antibody can also be added to the MagBeads to isolate your target protein using a direct or indirect method (Figure 5). 1 st -antibody (Cross-linking optional) target protein Add 1xSDS loading buffer and heat to 100 o C GenScript 2 nd Ab-coated MagBeads 1 st -antibody and target protein complex The direct method is preferred when the target protein is abundant: the primary antibody is firstly bound to the GenScript MagBeads, the Ab-bound are then incubated with your sample to capture the target protein. The indirect method is well suited when the primary antibody has a poor affinity for the target or when the target is in low-abundance: the primary antibody is firstly incubated with your sample to form an Ab-antigen complex; this complex is then captured by adding GenScript MagBeads to your sample, followed by magnetic separation. Isolate pure antibodies Denaturing elution for isolation of denatured protein (No cross-linking) Mild elution for isolation of native protein (1 st -Ab cross-linked to 2 nd -Ab) Figure 5. How GenScript Secondary Antibody Coated MagBeads Work.

6 Ordering Information Product Quantity Cat. No. Price Protein A MagBeads 4 ml L00273 $89.00 Protein G MagBeads L00274 $ Protein A/G MagBeads L00277 $ Ni-Charged MagBeads L00295 $98.00 Glutathione MagBeads L00327 $ Mouse Anti-His mab MagBeads L00275 $ Mouse Anti-GST mab MagBeads L00336 Mouse Anti-Trx mab MagBeads L00337 Goat Anti-Rabbit IgG MagBeads L00328 Donkey Anti-Goat IgG MagBeads L00332 Customer References Luo MH et al. Human Cytomegalovirus Infection Causes Premature and Abnormal Differentiation of Human Neural Progenitor Cells. J. Virol. 2010; 84(7): LO YS et al. Oriented immobilization of antibody fragments on Ni-decorated single-walled carbon nanotube devices. ACS Nano. 2009; 3(11): For more information, visit Beads Tel: / Toll-Free Tel: Fax: / product@genscript.com