biomapping FLAG Epitope System Original & Proven System for Demanding Applications

Transcription

1 biomapping FLAG Epitope System Original & Proven System for Demanding Applications

2 biomapping FLAG Epitope System Original & Proven System for Demanding Applications Overview A Proven System for Detection and Purfication of Proteins The FLAG expression system is an established way to express, purify and detect recombinant fusion proteins. FLAG and 3 FLAG have proven utility in numerous applications such as, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, protein-protein interactions, etc. These small hydrophilic tags facilitate superior detection and purification of recombinant fusion proteins when using our highly specific and sensitive ANTI-FLAG antibodies. Proven: Cited in more than 2,000 publications Ultrasensitive: Antibodies detect as little as 100 femtomoles of protein Comprehensive: Complete systems in multiple formats Ideal Eprotope Tags: Small, Hydrophilic and Cleavable The FLAG expression system utilizes a short, hydrophilic 8-amino acid peptide that is fused to the recombinant protein of interest. The FLAG peptide is easily accessible for cleavage by enterokinase (Ek) and for detection with antibodies, and is not likely to obscure other epitopes, domains, or alter function, secretion or transport of the fusion protein. The 3 FLAG system is an improvement upon the original system by fusing 3 tandem FLAG epitopes (22 amino acids). Detection of fusion proteins containing 3 FLAG is enhanced up to 200 times more than any other system. Like the original FLAG tag, 3 FLAG is hydrophilic, contains an Ek cleavage site, and is relatively small. Therefore, the risk of altering protein function, blocking other epitopes or decreasing solubility is minimized. Highly Sensitive FLAG; Detect 100 femtomoles 3xFLAG; Detect 10 femtomoles Western Blot of Original FLAG vs. 3xFLAG Femtomoles Fusion Protein 3 FLAG-BAP FLAG-BAP Western blot of purified 3 FLAG bacterial alkaline phosphatase and FLAG bacterial alkaline phosphatase transferred onto a nitrocellulose membrane. Detection was performed with ANTI-FLAG M2 mono clonal primary antibody, anti-mouse-hrp secondary antibody, and ECL chemiluminescent substrate. View recent literature at wherebiobegins.com/flaglit FLAG and 3xFLAG Amino Acid Sequences FLAG Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys Enterokinase Cleavage Site Protein 3 FLAG Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-IIe-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys Protein Enterokinase Cleavage Site Removal of N-terminal FLAG (8 amino acids) and 3 FLAG (22 amino acids) tags is possible using enterokinase, which cleaves following the Asp-Asp-Asp-Asp-Lys amino acid sequence at the C-terminal end of the tags.

3 Order Technical Service FLAG Ultrasensitive; times more sensitive than any other system Detect 10 femtomoles Ideal for cases of low-level expression in mammalian cells 3 tandem repeats of the FLAG sequence Enhanced detection for immunoprecipitation, Western blots and immuno cytochemistry Sensitivity Fold Increases in Sensitivity Over HA Tag xFLAG 6xHIS HA c-myc GST Highly Specific Antibodies FLAG and 3 FLAG sequences include the binding sites for several highly specific ANTI-FLAG monoclonal (M1, M2, M5), and polyclonal antibodies and conjugates, each with different recognition and binding characteristics ANTI-FLAG antibodies exhibit little or no cross-reactivity in most mammalian and bacterial cell lysates Detection Western blot detection of FLAG fusion protein with ANTI-FLAG M2 Antibody Lane 1: FLAG-BAP * control protein Lane 2: COS-7 Cell extract control Lane 3: pflag-cmv -2-BAP* transfected COS-7 cell extract *BAP: Bacterial alkaline phosphatase One Step Purification Single band purity with only one chromatography step Non-denaturing purification Simple, competitive elution using FLAG and 3 FLAG synthetic peptides Purification formats include affinity gels and 96-well plates Purification 1 2 SDS-PAGE of cell lysates from E. coli transfected with pflag-ats -BAP and purified with ANTI-FLAG M2 affinity gel (stained with Coomassie Brilliant Blue) Lane 1: Cell extract prior to purification Lane 2: Affinity purified FLAG-BAP* fusion protein Vectors for Bacterial and Mammalian Systems Cytoplasmic expression or secretion Bacterial vectors with T7 or tac promoters 3 FLAG, as well as FLAG, offered for mammalian systems Options for dual-tagged fusion proteins N-terminal FLAG and 3 FLAG vectors provide an Ek cleavage site for removal of the fusion tag BICEP line of vectors for bicistronic expression in mammalian cells offer high performance stable expression of FLAG fusions or multi-gene expression possibilities Purification Bacterial Expression Vectors ORF pflag Mammalian Expression Vectors

4 biomapping FLAG Epitope System Original & Proven System for Demanding Applications Vectors Bacterial Expression Vector Selection Table Cat. No. Product Description Promoter FLAG MAT OmpA Ek Site amp r kan r E5530 ptac-mat -Tag-1 tac N x E5405 ptac-mat-tag-2 tac C x E8158 ptac-ats tac N x x x E8408 pflag-ctc tac C x E8033 pflag-mac tac N x x P1118 pt7-flag-1 T7/lacO N x x P1243 pt7-flag-2 T7/lacO C x E5780 pt7/laco T7/lacO N x E5655 pt7/mat-tag-1 T7/lacO C x E5280 pt7-flag-mat-tag-1 T7/lacO N C x x E4905 pt7-mat-tag-flag-2 T7/lacO C N x E5155 pt7-mat-tag-flag-1 T7/lacO N N x x E5030 pt7-flag-mat-tag-2 T7/lacO C C x P9618 pt7-flag-3 T7/lacO N x x x P9743 pt7-flag-4 T7/lacO C x x N = N-terminal tag C = C-terminal tag OmpA = periplasmic localization Ek = Entrokinase cleavage site amp r = ampicillin resistance gene kan r = kanamycin resitance gene Mammalian Expression Vector Selection Table Transient Expression Cat. No. Product Description PPT FLAG 3xFLAG c-myc MAT Ek Site amp r E3762 pflag-cmv-5a,b,c C x E2275 pflag-cmv-6a,b,c N x x C6114 pcmv-flag-mat-tag-2 C C x E7029 pbicep-cmv-3 N (MCS1) x x E5905 pcmv-bicep -4 N (MCS1) N (MCS2) x x E7033 pflag-cmv-2 N x x E6908 pflag-cmv-5.1 C x x E7408 p3xflag-cmv-7 N x x E7533 p3xflag-cmv-7.1 x N x x E9908 p3xflag-cmv-8 x N x x E9158 p3xflag-cmv-23 x N C x x E9283 p3xflag-cmv-24 N C x x C5864 pcmv-flag-mat-1 N C x x N = N-terminal tag C = C-terminal tag PPT = preprotrypsin leader for direct secretion cmyc = c-myc epitope MAT = metal affinity tag amp r = ampicillin resistance gene Ek = Enterokinase clevage site (cleavage of dual-tagged proteins my result in removal of one or more tags)

5 Order Technical Service Mammalian Expression Vector Selection Table Stable Expression Cat. No. Product Description PPT FLAG 3xFLAG c-myc Ek Site amp r neo r E0779 pbicep-cmv-1 N (MCS1) x x x E0904 pbicep-cmv-2 N (MCS1) x x x E6783 pflag-cmv-3 x N x x x E7158 pflag-cmv-4 x N x x x E9783 p3xflag-cmv-9 x N x x x E7658 p3xflag-cmv-10 x N x x x E7783 p3xflag-cmv-13 x C x x x E7908 p3xflag-cmv-14 C x x x E9033 pflag-myc-cmv-22 x N C x x x E9408 p3xflag-myc-cmv-25 x N C x x x E7283 p3xflag-cmv-26 N C x x x N = N-terminal tag C = C-terminal tag neo r = neomycin resistance for stable isotope selection PPT = preprotrypsin leader for direct secretion cmyc = c-myc epitope amp r = ampicillin resistance gene Ek = Enterokinase clevage site (cleavage of dual-tagged proteins my result in removal of one or more tags) Insect Expression Vector Selection Table Stable Expression Cat. No. Product Description PPT FLAG 3xFLAG c-myc Ek Site amp r neo r T6824 ppolh-flag-1 N x E6155 ppolh-flag-2 C x N = N-terminal tag C = C-terminal tag neo r = neomycin resistance for stable isotope selection PPT = preprotrypsin leader for direct secretion cmyc = c-myc epitope amp r = ampicillin resistance gene Ek = Enterokinase clevage site (cleavage of dual-tagged proteins my result in removal of one or more tags) Tag Removal Cat. No. Product Description Application Product Image PRKE Enterokinase Removal Kit For removal of enterokinase by immunoaffinity from fusion protein cleavage reaction mixtures E5144 Enterokinase from Bovine Intestine - Powder Removes FLAG peptide from N-terminal and Met-N-terminal fusion proteins Enterokinase -X-Asp-Asp-Asp-Asp-Lys- -Not Proline-X-

6 biomapping FLAG Epitope System Original & Proven System for Demanding Applications Purification & Detection Cat. No. Product Description Characteristics Applications FLAG Affinity Gels A4596 ANTI-FLAG M1 Agarose Affinity Gel A2220 F2426 FLAG Peptides F3290 F4799 ANTI-FLAG M2 Agarose Affinity Gel EZview Red ANTI-FLAG M2 Affinity Gel FLAG Peptide 3xFLAG Peptide FLAG Antibodies F3040 ANTI-FLAG M2 Monoclonal Antibody, Purified IgG Specificity: N-terminal FLAG fusion proteins. Binding Ca 2+ -dependent; the complex dissociates in the absence of calcium ions. Does not bind to Met-FLAG fusion proteins, will not recognize unprocessed, cytoplasmically expressed proteins Binding Capacity: 0.6 mg protein per ml gel, binding requires Ca 2+ Elution: FLAG peptide; glycine, ph 3.5; EDTA Form: Suspension of beaded agarose in 50% glycerol containing 10 mm sodium phosphate, 150 mm NaCl, ph 7.4, 0.02% (w/v) sodium azide Specificity: N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3 FLAG fusion proteins Binding Capacity: 0.6 mg per ml gel Elution: FLAG peptide; glycine, ph 3.5; 3 FLAG Peptide Form: Suspension of beaded agarose in 50% glycerol containing 10 mm sodium phosphate, 150 mm NaCl, ph 7.4, 0.02% (w/v) sodium azide, freezer safe Specificity: N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins Binding Capacity: 0.6 mg protein per ml gel Elution: FLAG peptide; glycine, ph 3.5; 3xFLAG peptide Form: Suspension of red colored beaded agarose in phosphate buffered saline containing 50% glycerol and % Kathon CG/IPCII as an antimicrobial preservative Sequence: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys MW: 1013 Form: Lyophilized powder Sequence: Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr- Lys-Asp-Asp-Asp-Asp-Lys Note: The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide; the eight amino acids at the C-terminus make up the classic FLAG sequence (Asp- Tyr-Lys-Asp-Asp-Asp-Asp-Lys) MW: Form: Lyophilized powder Specificity: N-terminal FLAG. Binding is Ca 2+ - dependent; the complex dissociates in the absence of calcium ions. Does not bind to Met-FLAG fusion proteins; will not recognize unprocessed, cytoplasmically expressed proteins Form: Solution in 10 mm sodium phosphate, 150 mm NaCl, ph 7.4, containing 0.02% sodium azide Purification of N-terminal FLAG fusion proteins Purification of FLAG and 3xFLAG fusion proteins Elution of FLAG fusion proteins from the ANTI- FLAG M1 and M2 affinity resins Working Concentration: 100 μg/ml is commonly used to elute FLAG fusion proteins Elution of 3xFLAG fusion proteins from ANTI-FLAG M2 affinity gels Working Concentration: 100 μg/ml is commonly used to elute 3xFLAG fusion proteins Immunoprecipitation Immunocytochemistry EIA

7 Order Technical Service Cat. No. Product Description Characteristics Applications FLAG Antibodies continued F3165 ANTI-FLAG M2 Monoclonal Antibody, Purfied IgG Specificity: N-terminal, Met-N-terminal, Carboxy-terminal, or internal. Binding is not Ca 2+ -dependent Form: Solution in 10 mm sodium phosphate, 150 mm NaCl, ph 7.4, containing 0.02% sodium azide F1804 Monoclonal ANTI-FLAG M2, Specificity: N-terminal, Met-N-terminal, Carboxy-terminal, or internal. Binding is antibody produced in mouse, not Ca 2+ -dependent affinity purified Form: 50% glycerol solution, 10 mm sodium phosphate, and 150 mm NaCl, ph 7.4. The formulation contains no antimicrobial preservatives F4042 F2555 F7425 ANTI-FLAG M5 Monoclonal Antibody Rabbit Monoclonal ANTI- FLAG, ascites fluid Rabbit ANTI-FLAG Polyclonal Antibody FLAG Antibody Conjugates F9291 ANTI-FLAG BioM2 Antibody, Biotin Conjugate SAB SAB Monoclonal ANTI-FLAG antibody produced in rat Monoclonal ANTI-FLAG-Peroxidase antibody produced in rat Specificity: Met-N-terminal FLAG. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli. Binding is not Ca 2+ -dependent Form: Solution in 10 mm sodium phosphate, 150 mm NaCl, ph 7.4, containing 0.02% sodium azide Specificity: N-terminal and C-terminal FLAG fusion proteins Form: 50% glycerol solution, 10 mm sodium phosphate, and 150 mm NaCl, ph 7.4. The formulation contains no antimicrobial preservatives Specificity: Reacts with N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins. Binding is not Ca 2+ -dependent Form: Solution of affinity isolated antibody in 10 mm sodium phosphate buffered saline, ph 7.4, containing 1% bovine serum albumin and 15 mm sodium azide Specificity: N-terminal, Met-N-terminal or C-terminal of FLAG fusion proteins. Binding is not Ca 2+ -dependent Form: Solution in 50% glycerol, 10 mm sodium phosphate, 150 mm NaCl, ph 7.4, containing 0.02% sodium azide. The conjugate protein concentration is ~1 mg/ml Specificity: Recognizes N-terminal, C-terminal and internal FLAG-tagged fusion proteins. Form: Buffered aqueous solution Specificity: Recognizes N-terminal, C-terminal and internal FLAG-tagged proteins. Form: 0.01 M phosphate buffered saline, ph 7.4, containing 0.01% merthiolate EIA EIA EIA 1:1,000-1:2,000 by indirect EIA 5 μg/ml by indirect immunofluorescence 2.55 μg/ml by indirect immunofluorescence Immunoblotting Immunoblotting

8 biodescriptor Cat. No. Product Description Characteristics Applications A9469 ANTI-FLAG M2-Alkaling Phosphatase A8592 F4049 A9594 ANTI-FLAG M2-Peroxidase ANTI-FLAG M2 Monoclonal Antibody-FITC ANTI-FLAG M2-Cy 3 Conjugate Secondary Antibodies A9044 Rabbit Anti-Mouse IgG, (whole molecule) Peroxidase Conjugate A9917 Goat Anti-Mouse IgG, Fab Fragment Peroxidase Conjugate with Human IgG Specificity: N-terminal, Met-N-terminal or C-terminal of FLAG fusion proteins. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity. Binding is not Ca 2+ -dependent Form: Purified immunoglobulin solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative. The conjugate protein concentration is ~1 mg/ml Specificity: N-terminal, Met-N-terminal or C-terminal of FLAG fusion proteins. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity. Binding is not Ca 2+ -dependent Form: Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer. The conjugate protein concentration is ~1 mg/ml Specificity: N-terminal, Met-N-terminal or C-terminal of FLAG fusion proteins. Binding is not Ca 2+ -dependent. Form: Solution in 10 mm sodium phosphate, 150 mm NaCl, 1% bovine serum albumin and 0.1% sodium azide ph 7.4 Specificity: N-terminal, Met-N-terminal or C-terminal of FLAG fusion proteins. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity. Binding is not Ca 2+ -dependent. Form: Purified immunoglobulin in phosphate buffered saline plus 1% BSA and preservative. The conjugate protein concentration is ~1 mg/ml Specificity: Mouse IgG. Binds all mouse lgs Form: Solution in 0.01 M phosphate buffered saline, ph 7.4, containing 0.01% thimerosal as a preservative Specificity: Mouse IgG Fab. Immunospecific purification removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of mouse IgG. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross-reactivity in tissue or cell preparations. Form: Solution in 0.01 M phosphate buffered saline, ph 7.4, containing 0.01% thimerosal as a preservative ELISA 1:20,000 by indirect ELISA Immunohistochemistry ELISA 1:20,000 by indirect ELISA 1:100 1:1,000 by immunocytochemistry Fluorescent immunocytochemistry Fluorescent immunohistochemistry Flow cytometry immunofluorescence using mammalian cells fixed with methanol:acetone immunofluorescence using mammalian cells fixed with methanol:acetone 1:6,000 8,000 by dot blotting 1:40,000 by direct ELISA 1:200 by immunohistochemistry (formalin-fixed, parrifinembedded sections) 1:80,000 by indirect 1:8,000 by dot blotting 1:60,000 by direct ELISA 1:150 by immunohistochemistry (formalin-fixed, parrifinembedded sections) 1:80,000 by indirect

9 Order Technical Service Cat. No. Product Description Characteristics Applications A3682 Goat Anti-Mouse IgG, Fab Fragment Peroxidase Conjugate, Adsorbed with Human IgG and Rat Serum Proteins FLAG-Control Proteins P5975 Met-FLAG BAP Control Protein P7457 P7582 Carboxy-terminal Met-FLAG BAP Control Protein Amino-terminal Met-FLAG BAP Control Protein Multiwell Format P2983 ANTI-FLAG High Sensitivity Clear, M2 coated 96-well plate Magnetic Bead Format M8823 ANTI-FLAG M2 Magnetic Beads Specificity: Mouse IgG Fab. Immunospecific purification removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of mouse IgG. The antibody preparation is solid phase absorbed with human IgG to ensure minimal cross-reactivity in tissue or cell preparations. Binds all mouse lgs Form: Solution in 0.01 M phosphate buffered saline, ph 7.4, containing 0.01% thimerosal as a preservative N-terminal Met-FLAG BAP control protein is a 468 amino acid N-terminal Met- FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) MW: 49.4 kda A 466 amino acid C-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) MW: 49.1 kda A 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) MW: 49.3 kda Specificity: N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins. Detects as little as 1 ng/well of FLAG fusion protein Binding Capacity: 100 ng/well Specificity: Non-specific binding of 10% or less when the product is used to purify FLAG fusion proteins from mammalian and bacterial extracts Binding Capacity: 0.6 mg of FLAG fusion protein per 1 ml of packed magnetic beads Elution: FLAG peptide, 3x FLAG peptide Form: 50% slurry 1:4,000 by dot blotting 1:40,000 by direct ELISA 1:150 by immunohistochemistry (formalin-fixed, parrifinembedded sections) 1:80,000 by indirect Control protein for ANTI-FLAG M2 and M5 monoclonal antibodies in, ELISA, immunoprecipitation, fluorescence microscopy, light microscopy, FACS and in immunoaffinity chromatography with the ANTI-FLAG M2 affinity gel Control protein for the ANTI-FLAG M2 monoclonal antibody in immunological procedures such as, ELISA, immunoprecipitation, fluorescence microscopy, light microscopy, FACS and immunoaffinity chromatography Control protein for ANTI-FLAG M1 and M2 monoclonal antibodies in, ELISA, immunoprecipitation, fluorescence microscopy, light microscopy, FACS and in immunoaffinity chromatography Suitable for High- Throughput Screening, Immunoprecipitation and ELISA assays Suitable for magnetic automation platforms Purification of FLAG and 3x FLAG fusion proteins

10 biodescriptor FLAG Epitope System Original & Proven System for Demanding Applications Purification & Detection Kits FLAG M Purification Kit The FLAG M Purification Kit provides efficient lysis and protein extraction from mammalian cells. Affinity purification is performed with ANTI-FLAG M2 affinity gel to ensure high specificity of FLAG fusion proteins. Sufficient for 3 5 uses of 1 ml affinity purification column. High specificity and robust purification of FLAG fusion proteins Eliminate need for preliminary steps and calibrations Analyze target protein for activity, size, post-translational modifications and interactions Components CelLytic M 10x Wash Buffer Elution Buffer 3xFLAG Peptide ANTI-FLAG M2 Agarose Affinity Gel Amino-terminal FLAG-BAP Fusion Protein Polypropylene Chomatography Column Ordering Information Cat. No. CELLMM2 Product Description FLAG M Purification Kit FLAG HA Tandem Affinity Purification Kit The FLAG HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology. TAP technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification protein complexes by adding a tandem affinity tag to a known bait protein to pull down endogenous proteins that interact with the targeted protein of interest. Components EXview Red ANTI-FLAG M2 Affinity Gel Monoclonal Anti-HA-Agarose antibody produced in mouse RIPA buffer 3xFLAG Peptide Urea, 8M Ordering Information Cat. No. TP0010 Product Description FLAG HA Tandem Affinity Prufication Kit

11 Order Technical Service FLAG Epitope System Original & Proven System for Demanding Applications Purfication & Detection Kits ProteoQwest FLAG Colorimetric Western Blotting Kit, TMB Substrate The ProteoQwest FLAG Colorimetric Western Blotting Kit is optimized to eliminate non-specific background in comparison to the use of ANTI-FLAG antibodies with anti-mouse IgG secondary antibody peroxidase conjugates. The kit includes a purified FLAG fusion protein, N-FLAG-BAP to confirm proper performance and contains sufficient reagents for 25 mini-gel sized (10 10 cm) blots. Detect as little as 1 ng of FLAG fusion protein on Western blots Colorimetric reaction occurs directly on membrane Visualize results easily no darkroom, film or imager required Components N-FLAG-BAP Control Protein ANTI-FLAG M2-Peroxidase (HRP) Conjugate 3,3,5,5 -Tetramethylbenzidine (TMB) Substrate Ordering Information Cat. No. Product Description Quantity PQ0300 ProteoQwest FLAG Colorimetric Western Blotting Kit, 1 kit TMB Substrate The FLAG Immunoprecipitation Kit The FLAG Immunoprecipitation Kit provides rapid and efficient immunoprecipitation and elution of FLAG fusion proteins. Typically 70 90% of a bound FLAG fusion protein is released and retains biological activity. Highly specific detection with ANTI-FLAG M2 affinity gel No preliminary calibration steps required Efficient and gentle elution through direct competition using 3 FLAG peptide Components ANTI-FLAG M2 affinity gel Elution Buffer FLAG-BAP Control Protein 3xFLAG Peptide Lysis Buffer 2x Sample Buffer 10x Wash Buffer Ordering Information Cat. No. Product Description Quantity FLA GIPT1 FLAG Immunoprecipitation Kit 1 kit

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