Easy, Precise, Reliable Gel Electrophoresis Markers. Blocking Buffers. Ordering Information. Ordering Information

Transcription

1

2

3 Easy, Precise, Reliable Gel Electrophoresis Markers Rockland s G.E.M. product line offers a variety of prestained protein standards and DNA ladders for electrophoresis applications. Protein standards suitable for SDS- PAGE and western blot analysis, and DNA ladders for use in agarose gels, are provided as ready-to-use markers. All GEM products display an array of bands to eliminate molecular weight size ambiguity, while reducing the need for hands-on work. These broad range markers are ideal for use in a variety of gels with a variety of running buffers. The GEM product line is designed for ease, convenience and reliability. The ready-to-use format, combined with their stability at room temperature for 6 months, dramatically reduces the time required to prepare gel electrophoresis applications. Less work, more results. Sapphire: Blue protein standard. Ruby: Two-color protein standard. Opal: Three-color protein standards for a wide variety of molecular weights. VWR Cat. No. Product Description Pkg RLMB Ruby Prestained Protein Standard kDa 500 µl RLMB Opal Prestained Protein Standard kDa 500 µl RLMB Opal Prestained Protein Standard kDa 500 µl RLMB Opal Prestained Protein Standard kDa 500 µl RLMB Sapphire Prestained Protein Standard kDa 500 µl Blocking Buffers When performing a western blot, the blocking buffer should not be overlooked. The blocking buffer fills in the locations on the membrane that can still bind protein and cause background if not treated. The critical reagent in a blocking buffer is protein, where the protein is non-antibody reactive. VWR Cat. No. Product Description Pkg MB-070 Blocking Buffer for Fluorescent Western Blotting 500 ml B BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) 500 g BSA-10 Bovine Serum Albumin 10 g MB Revitablot Stripping Buffer 500 ml MB X SDS-PAGE Running Gel Buffer 1 L MB-018 2X SDS-PAGE Sample Buffer 100 ml 3

4

5 Epigenetic Antibodies Epigenetics studies the phenotypic regulation from "the top down", resulting in functional modifications to genetic material without altering the primary DNA sequence. Rockland epigenetic antibodies provide accurate and highly specific results. VWR Cat. No. Product Description Size RL E81 Anti-Histone H3 (RABBIT) Antibody 100 µl RL E81S Anti-Histone H3 (RABBIT) Antibody 25 µl RL C42 Anti-CTCF (RABBIT) Antibody 100 µg RL J21 Anti-HDAC5 (RABBIT) Antibody 100 µg RL J20 Anti-HDAC4 (RABBIT) Antibody 100 µg RL600401J22 Anti-HDAC7 (RABBIT) Antibody 100 µg RL N93 Anti-TET2 (RABBIT) Antibody 100 µl RL N93S Anti-TET2 (RABBIT) Antibody 25 µl RL N94 Anti-TET3 (RABBIT) Antibody 100 µl RL N94S Anti-TET3 (RABBIT) Antibody 25 µl Epi-Plus Antibodies Rockland now manufactures antibodies from the Epi-Plus epigenetics line, providing insight into the myriad of modifications that provide continuing guidance of gene expression. VWR Cat. No. Product Description Size RL600401I53 Anti-Histone H3 [Asym-dimethyl Arg2] (RABBIT) Antibody 50 µg RL600401I57 Anti-Histone H3 [Monomethyl Lys4] (RABBIT) Antibody 50 µg RL600401I59 Anti-Histone H3 [Trimethyl Lys4] (RABBIT) Antibody 50 µg RL600401I67 Anti-Histone H3 [Asym-dimethyl Arg8] (RABBIT) Antibody 50 µg RL600401I71 Anti-Histone H3 [Trimethyl Lys9] (RABBIT) Antibody 50 µg RL600401I74 Anti-Histone H3 [p Ser10] (RABBIT) Antibody 50 µg RL600401I83 Anti-Histone H3 [p Ser28] (RABBIT) Antibody 50 µg RL600401I86 Anti-Histone H3 [Monomethyl Lys36] (RABBIT) Antibody 50 µg RL600401I87 Anti-Histone H3 [Dimethyl Lys36](RABBIT) Antibody 50 µg RL600401I96 Anti-Histone H4 [p Ser1] (RABBIT) Antibody 50 µg 5

6

7 TrueBlot : Clear Detection of Blotted Target Bands Rockland s TrueBlot product line enables researchers to generate publication-quality western blot data for Nfkb IP Western Blots, Phospho IP Western Blots, and GFP IP Western Blots. Simply substitute your conventional HRP blotting reagent with Goat TrueBlot, Mouse TrueBlot, Rabbit TrueBlot or Sheep TrueBlot. TrueBlot is a horseradish peroxidase conjugated immunoblotting detection reagent, which enables unhindered detection of blotted target bands. This results in unparalleled clarity in results and high quality imaging suitable for reproduction. VWR Cat. No. Product Description Size RL TrueBlot Anti-Rabbit Ig IP Beads 2.5 ml RL TrueBlot Anti-Mouse Ig IP Beads 2.5 ml RL TrueBlot Anti-Goat Ig IP Beads 2.5 ml RL Mouse TrueBlot: Anti-Mouse Ig Biotin 25 µg RL Mouse TrueBlot: Anti-Mouse Ig Biotin 100 µg RL Goat TrueBlot: Anti-Goat IgG HRP 50 µl RL Goat TrueBlot: Anti-Goat IgG HRP 200 µl RL Sheep TrueBlot: Anti-Sheep IgG HRP 50 µl RL Sheep TrueBlot: Anti-Sheep IgG HRP 200 µl RL Rabbit TrueBlot: Anti-Rabbit IgG HRP 50 µl RL Rabbit TrueBlot: Anti-Rabbit IgG HRP 200 µl RL Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP 20 µl RL Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP 50 µl RL Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP 200 µl RL Goat TrueBlot Set (with IP beads) 1 Set RL Rabbit TrueBlot Set (with IP beads) 1 Set RL Mouse TrueBlot Set (with IP beads) 1 Set RL Goat TrueBlot Western Blot Kit 1 Kit RL Rabbit TrueBlot Western Blot Kit 1 Kit RL Mouse TrueBlot Western Blot Kit 1 Kit 7

8