Aviva Systems Biology

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1 Aviva Custom Antibody Service and Price Mouse Monoclonal Antibody Service Package Number Description Package Contents Time Price Customer provides antigen protein $6,174 Monoclonal package1 (From protein to ascites Monoclonal package2 (From peptide design to ascites Monoclonal package3 (From plasmid to ascites Protein will be checked by SDS-PAGE Immunize four mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which IgG isotype determination Ascites are prepared from two hybridoma Antigen affinity purification Dot blot for QC. Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize four mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which IgG isotype determination Ascites are prepared from two hybridoma Antigen affinity purification Dot blot for QC. Customer provides expression vector Express and purify the antigen protein Immunize four mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which 2-3mg peptides 1mg protein $6,790 $7,105 Tel: Fax:

2 Monoclonal package4 (From cdna to ascites Monoclonal package5 (Phospho- specific monoclonal antibodies) Monoclonal package6 (Western guaranteed IgG isotype determination Ascites are prepared from two hybridoma Antigen affinity purification Dot blot for QC. Customer provides gene and protein information We clone the gene and construct the expression vector for fusion protein Express and purify the antigen protein Immunize four mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which IgG isotype determination Ascites are prepared from two hybridoma Antigen affinity purification Dot blot for QC. Customer provides antigen peptide Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize six mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which IgG isotype determination Ascites are prepared from two hybridoma Antigen affinity purification Dot blot for QC. We guarantee ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, phospho-peptide 100 ng, but negative at antibody dilution 1:100, unphospho-peptide 100ng. Customer provides antigen peptide recombinant vector 1mg protein 2-3mg peptide including non- and phosphor-peptide specific for phosphorpeptide 2 for non-phospho-peptide phosphor-specific nonspecific antibody from two 2-3mg peptides $7,721 $8,645 $11,116 Tel: Fax:

3 monoclonal antibodies) Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, Immunize six mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which IgG isotype determination Ascites are prepared from two hybridoma Antigen affinity purification Western blot for QC. We guarantee the ELISA titer of 1:20,000 or better, and the antibody is suitable for Western blot. including ELISA, Western blot and isotype determination data Tel: Fax:

4 Miscellaneous Services Peptide service Peptide design (suggestion) 1-2 days Free Peptide synthesis 2 Inquire Peptide conjugation (KLH/BSA/OVA, 5mg) Protein service Gene synthesis 3-4 $2.52/aa Gene amplification (Template provided by customer, 1000bp) 2-3 Vector construction 2-3 recombinant selection protein expression and purification 2-3 Monoclonal antibody service Protein checked by SDS-PAGE $91 Pre-immune serum collection 1 day Immunization & serum collection (4 mice) 6-10 $805 ELISA identification $62 Fusion and hybridoma development $4,942 Isotype determination $154 Ascites production (3 mice) 3-4 $462 Roller bottle cell culture (1L) 5-7 Prescreen (ELISA) Prescreen (Western Blot) $154 Prescreen (Dot Blot) Clone maintenance (1 clone) $21 Clone storage (1 clone) 1week Antibody purification service Saturated ammonium sulfate precipitation ( 60ml) 3-5days $126 Protein A/G affinity purification ( 60ml) 3-5 days $182 Tel: Fax:

5 Antigen affinity column preparation Antigen affinity purification ( 60ml) 3-5 days Antibody identification service ELISA identification (1-10 samples) Cell extraction (1 sample) Tissue extraction (1 sample) Protein concentration assay (1 sample) Western Blot identification (1-8 samples) Dot Blot identification (1-10 samples) $62 IHC identification (section provided by customer,1 photo) Antibody labeling service HRP labeling ( 5mg) 1-2 Biotin labeling ( 5mg) 1-2 $560 Tel: Fax:

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