Expression. Labeling. Crystallization. Purification. 伯 森 生 物 科 技 股 份 限 公 司 Blossom Biotechnologies Inc. P. 8 P. 3 P. 5 P. 6 P. 6 P. 2 P. 7 P.
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1 伯 森 蛋 白 質 研 究 產 品 專 刊 ProteinResearchProductCatalog Expression Purification Crystallization Labeling P. 2 LEXSY Protein Expression System P. 3 P. 5 P. 6 P. 6 Affinity Size Exclusion Ion Exchange FPLC Accessories P. 7 P. 7 P. 7 Screening Kit Crystallography Starter Kit Detergents P. 8 FluoroSpin Protein Labeling and Purification Kit B 伯 森 生 物 科 技 股 份 限 公 司 Blossom Biotechnologies Inc. 網 址 : 客 服 專 線 : 台 北 台 南 花 蓮 台 北 (02) 新 (037) 台 中 (04) 台 南 (06) 雄 (07)
2 Expression LEXSY Protein Expression System LEXSY 是 德 商 Jena Bioscience 的 獨 家 蛋 白 質 表 現 系 統, 以 單 細 胞 原 蟲 為 宿 主, 兼 具 了 真 核 生 物 系 統 以 及 大 腸 桿 菌 系 統 的 優 點, 是 您 大 量 表 現 蛋 白 的 最 佳 選 擇! LEXSY 特 點 : þ 不 具 包 涵 體 (inclusionbody) þ 後 修 飾 (post-translationalmodification) 近 似 哺 乳 物 細 胞 þ 操 作 流 程 條 件 穩 定 þ 表 現 量, 產 量 þ 不 具 物 毒 性 (Biosafetylevel1) LEXSY 完 美 克 服 其 他 蛋 質 表 現 系 統 常 問 題 Bacteria Yeast Insect & Mammalian cells Cultured Leishmania tarentolae cells expressing green uorescent protein. 產 蛋 質 無 後 修 飾 無 法 摺 疊 成 正 確 的 構 形 具 包 涵 體 後 修 飾 與 哺 乳 物 差 異 細 胞 不 易 破 壞, 抽 取 蛋 質 困 難 表 現 量 低 速 率 緩 慢 培 養 液 價 格 昂 貴 LEXSY grows faster than insect and mammalian cells LEXSY strains grow higher densities than insect and mammalian cells (Fritsche et al. 2007) LEXSY performs mammalian type of glycosylation (Breitling et al. 2002) Bacteria Yeast e.g. Pichia Insect cells e.g. Sf9/21 LEXSY Mammalian cells Cat. No. JB-EGE-1300 JB-EGE-1410 JB-EGE-1420 JB-EGE Product Name LEXSYcon2 Expression Kit, for constitutive protein expression LEXSinduce3 Expression Kit, for inducible expression from integrative constructs LEXSinduce4 Expression Kit, for inducible expression from episomal constructs LEXSY in vitro Translation Kit, for plasmid based cell-free protein synthesis 2
3 Purification FPLC 純 化 與 管 柱 伯 森 公 司 擁 有 完 整 的 FPLC 純 化 商 品, 包 含 親 和 性 層 析 過 濾 離 子 交 換 層 析 等, 提 供 來 自 德 國 瑞 典 與 日 本 的 優 良 產 品 各 式 產 品 均 擁 有 極 高 性 價 比, 歡 迎 進 一 步 索 取 相 關 資 料 及 免 費 試 用 服 務! Affinity 親 和 性 層 析 His-tag 重 組 蛋 白 純 化 重 流 磁 珠 預 注 管 柱 ABT-6BCL-NTANi-10 ABT-6BCL-NTANi-100 ABT-6BCL-NTANi-500 CU CU ABT-6BCL-QHNi-C8 ABT-6BCL-QHNi-C5 MF-HIS ML MF-HIS ML BW BW BW BW ABT-AF6NI-CTG5-2 ABT-AF6NI-CTG5-5 NICKEL NTA Agarose Resin, 10 ml NICKEL NTA Agarose Resin, 100 ml NICKEL NTA Agarose Resin, 500ml PureCube Ni-NTA Agarose, 10 ml PureCube Ni-NTA Agarose, 50 ml His-COLUMN HIGH Density NICKEL (8x1 ml) HisXL-COLUMN HIGH Density NICKEL (5x5 ml) Qbeads-NTA-Ni (2x1 ml) Qbeads-NTA-Ni (5x1 ml) BabyBio Ni-NTA (5x1 ml) BabyBio Ni-NTA (10x1 ml) BabyBio Ni-NTA (5x5 ml) BabyBio Ni-NTA (10x5 ml) NICKEL Af nity Cartridges 5ml (2x5 ml) NICKEL Af nity Cartridges 5ml (5x5 ml) NI Sepharose 6FF NI Sepharose 6FF NI Sepharose 6FF NI Sepharose HP NI Sepharose HP His Mag Sepharose His Mag Sepharose Histrap HP Histrap HP Histrap HP Histrap HP Histrap FF Histrap FF His-tag 重 組 蛋 白 偵 測 His Detection Kit l 快 速 偵 測 His Tag 重 組 蛋 白 純 化 結 果 l 不 需 Blocking, ing 等 步 驟 l 偵 測 極 限 可 達 0.1 pmole l 肉 眼 即 可 觀 察, 也 可 再 使 用 儀 器 定 量 Cat. No. JB-PS110 Product Name His Detection Kit Detection of poly-histidine tagged proteins, ready-to-use solution Up to 3 x 3 reactions Western Blot 1 hr Transfer 1-15 hr Block 30' 1-2 hr 1st Ab 30' 1-2 hr 2nd Ab 30' 5' Develop TOTAL 6-21 hr In-Gel Stain 1 hr Fix Gel 20' 1 hr Stain 20' TOTAL 3 hr His Detection Kit 1 hr Transfer 30'-90' Incubation TOTAL hr >5XFasterThanWesternBlot! 3
4 Purification GST-tag 重 組 蛋 白 純 化 磁 珠 預 注 管 柱 Tag 切 除 ABT-4B-GLU-10 ABT-4B-GLU-100 CU CU CU CU CU CU BML-SE Glutathione Agarose Resin, 10 ml Glutathione Agarose Resin, 100ml PureCube Glutathione Agarose, 10 ml PureCube Glutathione Agarose, 50 ml PureCube Glutathione MagBeads (5 ml) PureCube Glutathione Cartridge (5x1 ml) PureCube Glutathione Cartridge (1x5 ml) PureCube Glutathione Cartridge (5x5 ml) Thrombin Glutathione Sepharose 4B Glutathione Sepharose 4B Glutathione Sepharose HP Glutathione Sepharose HP GSTrap HP GSTrap HP GSTrap HP 抗 體 純 化 磁 珠 預 注 管 柱 BB-02-01PA-01 BB-02-01PG-01 ABT-4BCLPL-R5 MF-PRA ML MF-PRA ML MF-PRA ML MF-PRG ML MF-PRG ML MF-PRG ML BW BW BW BW Protein A agarose, 10 ml Protein G agarose, 10 ml Protein L agarose, 5ml Qbeads-Protein A, 2ml Qbeads-Protein A, 5ml Qbeads-Protein A, 10ml Qbeads-Protein G, 2ml Qbeads-Protein G, 5ml Qbeads-Protein G, 10ml BabyBio proteina (5x1 ml) BabyBio proteina (10x1 ml) BabyBio proteina (5x5 ml) BabyBio proteina (10x5 ml) nprotein A Sepharose Protein G Sepharose Protein L sepharose Protein A Mag Sepharose Xtra 5 Protein A Mag Sepharose Xtra 5 Protein A Mag Sepharose Xtra 5 Protein G Mag Sepharose Xtra 5 Protein G Mag Sepharose Xtra 5 Protein G Mag Sepharose Xtra 5 Hitrap protein A HP Hitrap protein A HP Hitrap protein A HP Hitrap protein A HP 其 它 親 和 性 層 析 純 化 磁 珠 ABT-6RRF-STV-5 CHI CU CU MF-STA ML MF-STA ML MF-DEX ML MF-COO ML MF-NHH ML Streptavidin Agarose, 5ml Cellu ne sulfate PureCube NHS Activated Agarose, 10ml PureCube Rho1D4 Agarose, 5ml Qbeads-Streptavidin Qbeads-Streptavidin Qbeads-Hydroxyl Qbeads-Carboxyl Qbeads-Amine Streptavidin Sepharose Heparin Sepharose NHS Sepharose Streptavidin Mag Sepharose Streptavidin Mag Sepharose 4
5 Purification Size Exclusion 過 濾 去 鹽 與 置 換 緩 衝 液 離 重 流 CP-0201-Z CP-0201-Z CP-0205-Z CP-0205-Z CP-0202-Z CP-0202-Z TM-0102-E TM-0102-E TM-0105-E TM-0105-E TM-0106-E CP-0109-Z CP-0104-Z CP-0105-Z Z-50 MINI Spin Columns, 25pk Z-50 MINI Spin Columns, 100pk Z-25 MINI Spin Columns, 25pk Z-25 MINI Spin Columns, 100pk SEQ Z-50 MINI Spin Columns, 25pk SEQ Z-50 MINI Spin Columns, 100pk Zetadex-25 Fine, 100g Zetadex-25 Fine, 500g Zetadex-50 Fine, 100g Zetadex-50 Fine, 500g Zetadex-50, 100g N2 Gravity Columns (1-200 µl), 50pk N10 Gravity Columns (0.5-1 ml), 50pk N25 Gravity Columns (1-2.5 ml), 25pk Microspin G-50 columns Microspin G-50 columns SpinTrap G-25 SpinTrap G-25 AUTOSEQ G-50 AUTOSEQ G-50 Sephadex G-25 ne Sephadex G-25 ne Sephadex G-50 ne Sephadex G-50 ne Sephadex G-50 DNA Grade MiniTrap G-25 PD-10 Desalting Columns PD-10 Desalting Columns Gel Filtration 過 濾 EMP-TM-0501-E EMP-TM-0501-E ABT-A-1020S-1000 ABT-A-1040S-1000 ABT-A-1060S-1000 ABT-A-1021S-1000 ABT-A-1041S-1000 ABT-A-1061S-1000 Zetadex 20-LH, 100g Zetadex 20-LH, 500g 2% B Agarose Bead 4% B Agarose Bead 6% B Agarose Bead 2% BCL Agarose Bead 4% BCL Agarose Bead 6% BCL Agarose Bead Sephadex LH-20 Sephadex LH-20 Sepharose 2B Sepharose 4B Sepharose 6B Sepharose CL-2B Sepharose CL-4B Sepharose CL-6B BabyBio Ni-NTA Mini Columns Z-25 and Z-50 MINI Spin Columns N2, N10, and N25 Gravity Columns 5
6 Purification Ion Exchange 離 子 交 換 預 注 管 柱 CHI CHI CHI CHI CHI CHI CHI CHI BW BW BW BW BW Cellu ne Q, 100ml Cellu ne S, 100ml Cellu ne A, 100ml Cellu ne C, 100ml Cellu ne Q (5x1 ml) Cellu ne S (5x1 ml) Cellu ne A (5x1 ml) Cellu ne C (5x1 ml) BabyBio Q (5x5 ml) BabyBio S (5x1 ml) BabyBio S (5x5 ml) BabyBio DEAE (5x1 ml) BabyBio DEAE (5x5 ml) Q Sepharose SP Sepharose DEAE Sepharose CM Sepharose Hitrap Q FF Hitrap SP FF Hitrap DEAE FF Hitrap CM FF Hitrap Q HP Hitrap S HP Hitrap S HP Hitrap DEAE HP Hitrap DEAE HP FPLC Accessories 純 化 配 件 空 管 柱 磁 座 C-50 CXL-50 MC-25 MC-100 ABT-FPLC8-3 ABT-FPLC30-2 ABT-FPLC45-2 ABT-FPLC80-1 O-MAGRACK PLASTIC columns, 12ml PLASTIC XL columns, 35ml PLASTIC MINI columns, 25pk PLASTIC MINI columns, 100pk EMPTY FPLC 8ml Columns, 3pk EMPTY FPLC 30ml Columns, 2pk EMPTY FPLC 45ml Columns, 2pk EMPTY FPLC 80ml Columns, 1pk Mag Rack (1.5 ml x 8 well) COLUMN PD-10,EMPTY Microspin columns Microspin columns Cellu ne Chromatography Media PLASTIC Columns (12ml & 35ml) Mag Rack 6
7 Crystallization Screening Kit 蛋 白 質 結 晶 條 件 多 如 繁 星,JBScreen 系 列 商 品 幫 助 實 驗 者 快 速 篩 選 出 最 適 宜 的 結 晶 條 件! JBScreen Classic JBScreen Basic JBScreen JCSG++ JBScreen Kinase JBScreen PEG/Salt 提 供 240 種 最 常 見 的 緩 衝 液 與 沉 澱 劑 之 組 合 整 理 出 學 者 研 究 最 容 易 成 功 的 96 種 條 件, 廣 泛 的 ph 範 圍 多 種 沉 澱 劑 由 JCSG 機 構 發 表, 以 稀 疏 矩 陣 為 原 理 的 篩 選 組 合 針 對 Protein Kinase 的 初 期 結 晶 所 設 計, 涵 蓋 了 96 種 常 見 結 晶 條 件 對 於 PEG 3350 與 PEG 5000 MME 與 48 種 鹽 類 組 合 的 條 件 篩 選 請 前 往 下 列 網 址 瀏 覽 完 整 產 品 項 目, 或 使 用 QR code 以 獲 得 進 一 步 資 訊 Crystallography Starter Kit l 包 含 一 系 列 實 用 工 具, 讓 使 用 者 以 省 時 省 力 的 方 式 獲 取 結 晶 l MicroMounts 具 獨 家 光 圈 設 計, 減 少 取 結 晶 時 多 餘 液 體 l 相 容 於 常 見 X 光 設 備, 並 具 極 低 X 光 背 景 值 l 堅 固 耐 用, 即 使 在 低 溫 條 件 下 也 不 變 形 Each kit contains 20 Dual-Thickness MicroMounts (M2-L18SP-A2), with 5 each of 75, 100, 150 and 200 µm apertures. forsampleretrieval Cat. No. JB-CSK-2 Product Name Crystallography Starter Kit, with everything to mount and collect X-ray data from your crystals Detergents Features Cat. No. Product Name 純 度 >99.5% 專 供 結 晶 學 使 用 CU CU CU CU CU CU CU CU CU CU CU Octylglucopyranoside (OG) (5x5 g) N,N-Dimethyl-n-dodecylamine N-oxide (LDAO) (5x5 g) n-decyl-beta-maltoside (DM) (5x5 g) n-dodecyl-beta-maltoside (DDM) (5x5 g) n-octyl-1-thio-beta-d-glucopyranoside (OTG) (5x5 g) n-decylphosphocholine (Fos-Choline 10) (5x5 g) n-dodecyl-phosphocholine (Fos-Choline 12) (5x5 g) n-tetradecylphosphocholine (Fos-Choline 14) (5x5 g) n-hexadecylphosphocholine (Fos-Choline 16) (5x5 g) Detergent Screening Set Classic (1 g each of OG, LDAO, DM, DDM, OTG) Detergent Screening Set Phosphocholines 1 (1 g each of Fos-12, Fos-14, and Fos-16) 7
8 Labeling FluoroSpin Protein Labeling and Purification Kit FluoroSpin 能 將 廣 泛 的 螢 光 物 質 與 大 於 25 kda 的 抗 體 酵 素 與 其 它 蛋 白 質 快 速 地 接 合 與 純 化 其 特 點 如 下 : 市 上 最 快 速 的 ProteinLabelingKit! l 快 速 標 定 加 上 純 化,10 分 鐘 就 完 成 l 簡 單 只 要 三 步 驟 :Mix, Incubate, Purify l 精 確 可 精 確 控 制 蛋 白 質 標 定 的 程 度 l 方 便 產 品 內 已 包 含 Spin Columns 10 分 鐘 內 完 成 標 定! 8
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