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1 prosci-inc.com Custom Antibody Services High Performance Antibodies and More Broad Antibody Catalog Extensive Antibody Services

2 CUSTOM ANTIBODY SERVICES Established in 1998, ProSci Incorporated is a leading provider of high performance antibodies and custom antibody services. We have produced more than 10,000 custom monoclonal and polyclonal antibodies for researchers worldwide in academia, biotechnology, diagnostics, and pharmaceutical industries. Operating our own USDA licensed and NIH/OLAW assured animal facility in San Diego, California and performing immunological services in-house allows us to tailor antibody production for almost any antibody need. We look forward to assisting you with any aspect of the project from selecting the ideal type of antibody and host species to helping design an immunization protocol or an antigenicity-guaranteed peptide for your custom antibody project. ProSci has the experience and expertise to help you develop the antibodies you desire. Why choose ProSci for your custom service projects? Flexible service packages at competitive prices In-house labs and animal facilities in San Diego, CA (USA) Confidentiality of your work is important to us You retain the rights to any products from our services Capable of supporting large or small projects 2

3 Polyclonal Services Serum package Peptide package Purified package Rabbit Chicken Mouse Rat Guinea Pig Goat Llama Monoclonal Services Hybridoma development Ascites antibody production In vitro antibody production Call for Custom Schedules Additional Services Antibody purification Antibody labeling Complementary peptide antigen design assistance USDA License Number: 93-R-0521 NIH Animal Welfare Assurance Number: A TRACES Number: CA-INT-0005 CUSTOM ANTIBODY SERVICES prosci-inc.com/cas 3

4 POLYCLONAL ANTIBODY SERVICES New to Custom Antibodies? ProSci offers three simple packages, which may be customized to your needs. RABBIT STANDARD POLYCLONAL PACKAGES Item Serum Package Peptide Package Purified Package Antisera Schedule Peptide Synthesis 3 3 Peptide Conjugation 3 3 ELISA Report 3 3 Affinity Purification 3 Rabbit Chicken Mouse Rat Guinea Pig Goat Llama Pre-optimized Schedules for each Host Species. Post-Translational Modification Specific Polyclonal Services. POLYCLONAL ANTIBODY SERVICES prosci-inc.com/pas 4

5 MONOCLONAL ANTIBODY SERVICES Hybridoma Development Phase I: Immuniza on 5 BALB/c mice ELISA screening Phase II: Fusion 1 Mouse spleen ELISA screening Phase III: Subcloning 2 Subcloning steps ELISA screening Each of Our Hybridoma Development Packages Includes Isotyping of the final clones Cell line banking for 1 year following final clone shipment Monoclonal Antibody Production Ascites production In vitro production Additional Options Multiple ELISA screening Additional screening in WB or ICC Researcher screening of positive wells following fusion MONOCLONAL ANTIBODY SERVICES prosci-inc.com/mas 5

6 ADDITIONAL SERVICES Antibody Purification We purify samples that are produced internally or from those provided by customers via Protein A or G Immuno-affinity IgY purification Agarose Protein A/G Antibody Labeling Biotin HRP... And More Antibody Characterization Western blot services using our broad catalog of cell and tissue lysates Call for other antibody testing services ADDITIONAL SERVICES prosci-inc.com/additional-services 6

7 REFERENCES Select Citations J Bacteriol (2013) 195 (14): (Rabbit) Mol Cell Biol (2013) 33 (19): (Rabbit) Proc Natl Acad Sci USA (2013) 110 (49): (Rabbit) Appl Environ Microbiol (2012) 78 (8): (Mouse) J Biol Chem (2012) 287 (10): (Rabbit) J Neurosci (2012) 32 (15): (phospho Rabbit) J Clin Microbiol (2011) 49 (4): (Mouse) J Virol (2011) 85 (4): (Rabbit) Nucleic Acids Res (2011) 39 (21): (phospho Rabbit) J Neurosci (2010) 30 (42): (Rabbit) Proc Natl Acad Sci USA (2010) 107 (42): (Mouse Monoclonal) Plant Physiol (2009) 151 (1): (Rabbit) Am J Physiol Lung Cell Mol Physiol (2008) 294 (6): L (Goat) J Immunol (2008) 181 (2): (Goat) Mol Cancer Ther (2008) 7 (10): (Chicken) J Cell Biol (2007) 179 (6): (Rabbit) J Neurosci (2007) 27 (6): (Goat) J Biol Chem (2006) 281 (3): (Sheep) J Cell Sci (2006) 119 (Pt 16): (Rabbit) J Exp Med (2005) 202 (7): (Rabbit) Infect Immun (2004) 72 (8): (Chicken) Development (2002) 129 (24): (Rabbit) Our lab at UCSF has used many of ProSci s custom antibody preparation services, including the production of mouse monoclonal antibodies as well as the production of rabbit and goat polyclonal sera. ProSci helped at every step of the process, including synthetic peptide production and KLH coupling, developing fusion strategies and customizing monoclonal antibody screening options. ProSci consistently maintained high standards of customer service and offered very personalized attention. Working with ProSci truly helped our laboratory meet all its antibody needs. - Mark Singer, Specialist University of California, San Francisco We routinely use ProSci for polyclonal antibody production and have great results. The service is great and the antibodies speak for themselves. - Sara Cherry, Ph. D. Associate Professor University of Pennsylvania CUSTOM ANTIBODY SERVICE REFERENCES prosci-inc.com/service-references 7

8 prosci-inc.com Corporate Headquarters ProSci Incorporated Flint Place Poway, CA USA Contact Information Toll Free: (888) Local: (858) Fax: (858) High Performance Antibodies and More In-house USA Labs & Animal Facilities Established in

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