Choose your optimal tools for protein studies

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1 Protein Purification Choose your optimal tools for protein studies Bacterial Baculoviral Cell free Mammalian Secreted Intracellular High yield Increased solubility Highest purity Highest yield His-tag GST-tag DYKDDDDK-tag HAT tag Myc-tag HA-tag Fluorescent Proteins

2 H I S TA G G E D P R O T E I N P U R I F I C AT I O N Talon Co Resin-Highest Purity Advantages Applications Highest purity Crystallography Highest specificity for his-tagged proteins Functional assays Reduced co-purification of impurities Structural & functional investigations TALON resin is designed to maximize your yield of biologically active protein. Choose TALON when purity is of utmost importance or when purifying under native conditions Time (min) Elute Wash Load Elute Time (min) 0.03 Contaminant peak coelutes 0.01 Ni-NTA 0.04 A475 (AU) 0.02 Wash Load 0.03 Contaminant peak is separated TALON 0.04 A475 (AU) Each TALON reactive core is charged with a cobalt ion that has a much higher selectivity for histidine tags than nickel ions. Only proteins containing adjacent histidines or specially positioned histidines are able to bind to TALON. In contrast, the spatial requirement for nickel-based resins is less strict, so using Ni-NTA resins often results in co-purification of contaminants (see figure) IMAC purification TALON vs. Ni-NTA. 6xHN-AcGFP1 was purified from Sf21 cells using TALON or Ni-NTA. The chromatogram for each column is shown. The absorbance at 475 nm indicates the amount of target protein (AcGFP1) present in each fraction. His60 Ni Superflow Resin-Highest Capacity Advantages Applications Highest binding capacity (60 mg/ml) Protein labeling Purify proteins under native or denaturing conditions Immunization to produce antibodies Low leakage of Ni ions Animal studies 2+ His60 Ni Superflow Resin is a high-capacity resin for the efficient purification of his-tagged proteins. The resin enables fast, easy, and reproducible chromatographic separations and can be re-used for the same protein. His60 Ni Resin is compatible with batch/gravity-flow applications as well as with the major automated liquid chromatography systems and manual syringe processing. In one application, His60 Ni Superflow Resin performance was compared to the performance of Competitor Q s Ni Superflow resin. 6xHN-AcGFP1 was purified from equivalent amounts of the same sample, following each manufacturer s protocol. Clontech s pecoli Linear Expression System was used to express 6xHN-AcGFP1 in E. coli. Higher yields & better purity were obtained using our His60 Ni Superflow Resin (see figure). A kd His60 Ni Resin M OS FT W1 W2 E1 E2 E3 E Higher purity Higher yield xHN-AcGFP B kd NI-NTA Superflow Resin M OS FT W1 W2 E1 E2 E3 E4 Lower purity Lower yield xHN-AcGFP1 His60 Ni Superflow Outperforms Ni-NTA. Superior yields and better purity were obtained when comparing His60 Ni Superflow (Panel A) to Competitor Q s Ni resin (Panel B).

3 H I S TA G G E D P R O T E I N P U R I F I C AT I O N TALON Resin and Magnetic Beads TALON Resin Use for batch/gravity applications Highest purity of your target protein TALON Superflow Resin Use for FPLC applications Highest purity of your target protein TALON CellThru Resin Purify proteins directly from crude cell lysates, sonicates, and fermentation liquids Ideal for purification of membrane-bound proteins and multiprotein complexes TALON Magnetic Beads Microscale purification or screening of his-tagged proteins (bead particle size: µm) Elute in small volumes ( µl) His60 Ni Resin His60 Ni Superflow Resin Use for batch/gravity and FPLC applications Highest binding capacity 60 mg/ml Superior performance compared to other Ni-based resins (highest capacity and better purity) Low nickel ion leakage Purify under native or denaturing conditions Many sizes are available, from 10 ml to 1,000 ml Buffers and Detection Reagents Buffers HisTALON Buffer Set: complete buffer set for extraction and purification of his-tagged proteins using TALON resin His60 Ni Buffer Set: complete buffer set for extraction and purification of his-tagged proteins using His60 Ni Superflow resin xtractor Buffer: optimized lysis buffer for recombinant protein extraction xtractor Buffer Kit: complete extraction kit with lysozyme and DNase I, for efficient extraction of high molecular weight proteins Detection of His-Tags Antibodies 6xHis mab-hrp conjugate 6xHis Monoclonal Antibody (Albumin Free) 6xHN Polyclonal Antibody

4 H I S T A G G E D P R O T E I N P U R I F I C A T I O N 1 ml & 5 ml Cartridges FPLC Cartridges HisTALON & His60 Ni HisTALON Superflow His60 Ni Superflow Ready-to-use 1 ml and 5 ml cartridges Use on any chromatography system Simple manual operation using a syringe Can be connected in series to scale-up HisTALON Gravity Columns His60 Ni Superflow Gravity Columns HisTALON & His60 Ni Ready-to-use 1 ml columns Up to 60 mg pure his-tagged protein per His60 Ni Superflow column Up to 20 mg highly pure his-tagged protein per HisTALON column TALON Single Step Columns 5 ml 20 ml Single Step Columns TALON Single Step Ready-to-use 5 ml and 20 ml columns Load bacterial culture directly onto column On-column extraction and purification Load bacterial culture, wash, and elute target protein TALONSpin Columns Disposable Spin Columns TALONSpin Ready-to-use prepacked spin columns for small-scale purification Rapid, parallel purification in as little as 30 minutes Applications: screening of positive clones, trial-level purification TALON 2 ml Disposable Gravity Columns Empty 2 ml Disposable Columns TALON Ready-to-use empty 2 ml gravity columns Fill columns with TALON, TALON Superflow, or His60 Ni Superflow resins Also suitable for glycoprotein enrichment, phosphoprotein & phosphopeptide enrichment, GST-tag, and antibody purification resins CellThru 10 ml Disposable Columns Empty 10 ml Disposable Columns CellThru Ready-to-use empty 10 ml gravity columns Fill columns with TALON CellThru, TALON, TALON Superflow, or His60 Ni Superflow resins Also suitable for glycoprotein enrichment, phosphoprotein & phosphopeptide enrichment, and GST-tag & antibody purification resins

5 RÉFÉRENCE DÉSIGNATION CONDITIONNEMENT PRIX* 2014.HT Résines His60 (Ni) Résines en vrac et kits His60 Ni Superflow Resin 10 ml 106, His60 Ni Superflow Resin 25 ml 222, His60 Ni Superflow Resin 4 x 25 ml 776, His60 Ni Superflow Resin 250 ml 1 885, His60 Ni Superflow Resin 2 x 250 ml Nous consulter His60 Ni Superflow Resin 4 x 250 ml Nous consulter His60 Ni Magnetic Beads 1 ml 135,00 Colonnes prépackées (compatibles gravité) et kits His60 Ni His60 Ni Superflow Gravity Columns 5 colonnes 79, His60 Ni His60 Ni Superflow Gravity Column Purification Kit 5 colonnes / 20 purifications 293,00 Colonnes prépackées (compatibles FPLC) et kits His60 Ni Superflow Cartridge Purification Kit (5 x 1 ml) 20 purifications 420, His60 Ni Superflow Cartridges (5 x 1 ml) 5 cartridges 182, His60 Ni Superflow Cartridge Purification Kit (5 x 5 ml) 5 purifications 689, His60 Ni Superflow Cartridges (5 x 5 ml) 5 cartridges 486, His60 Ni Superflow Cartridge (1 x 5 ml) 1 cartridge 128,00 Résines TALON (Co) Résines en vrac TALON Metal Affinity Resin 10 ml 126, TALON Metal Affinity Resin 25 ml 270, TALON Metal Affinity Resin 100 ml 875, TALON Metal Affinity Resin 250 ml 1 909, TALON Metal Affinity Resin 2 x 250 ml Nous consulter TALON Metal Affinity Resin 4 x 250 ml Nous consulter TALON Superflow Metal Affinity Resin 25 ml 310, TALON Superflow Metal Affinity Resin 100 ml 1 087, TALON Superflow Metal Affinity Resin 4 x 250 ml Nous consulter TALON Superflow Metal Affinity Resin 2 x 250 ml Nous consulter TALON Superflow Metal Affinity Resin 250 ml 2 132, TALON CellThru 10 ml 151, TALON CellThru 100 ml 1 001, TALON Magnetic Beads 2 x 1 ml 210, TALON Magnetic Beads 6 x 1 ml 582,00 Colonnes prépackées (compatibles gravité) et kits TALON Single Step Columns (5 ml) 25 colonnes 366, Talon Single Step Columns (5 ml) 2 colonnes 75, TALON Single Step Columns (20ml) 10 colonnes 391, HisTALON Gravity Columns Purification Kit 5 colonnes / 20 purifications 330, HisTALON Gravity Columns 5 colonnes 118,00 Colonnes prépackées (compatibles centrifugation) TALONspin Columns 10 colonnes 149, TALONspin Columns 25 colonnes 290, TALONspin Columns 50 colonnes 416,00 Colonnes prépackées (compatibles FPLC) et kits HisTALON Cartridge Purification Kit (5 x 1 ml) 20 purifications 366, HisTALON Cartridge (5 x 1 ml) 5 cartridges 180, HisTALON Superflow Cartridge Purification Kit (5 x 5 ml) 5 purifications 751, HisTALON Superflow Cartridges (5 x 5 ml) 5 cartridges 619, HisTALON Superflow Cartridge (1 x 5 ml) 1 cartridge 146,00 Colonnes vides CellThru 10 ml Disposable Columns 20 colonnes 130, TALON 2 ml Disposable Gravity Column 50 colonnes 123,00 Tampons et détection His60 Ni & TALON Co Liste de prix Informations & Prix en ligne : * Prix susceptibles d'être modifiés selon le tarif en vigueur à la date de réception de la commande HisTALON Buffer Set 20 purifications 238, His60 Ni Buffer Set 20 purifications 249, TALON Xtractor Buffer Kit le kit 355, TALON Xtractor Buffer 500 ml 370, TALON Xtractor Buffer 100 ml 112, xHis mab-hrp Conjugate 100 µl 377, xHis Monoclonal Antibody (Albumin Free) 200 µg 605, xHN Polyclonal Antibody 200 µl 594,00

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