LIMBO CRITICALITY DETECTION WHU-CHINA 2015
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1 LIMBO CRITICALITY DETECTION WHU-CHINA 2015
2 Limbo wanders between lightness and darkness The Criticality Detector marks each cross
3 CRITICALITY DETECTION Signal Filter Simplify Signals Improve Sensibility
4 MAIN CIRCUIT CRITICALITY DETECTOR Red Light-Dark Sensor Negative Feed Back
5 Red Light Input Dark Sensor pcya ho1 cph8 Output C1 GFP tarna OmpC Binding site terminator OmpC terminator C1 R OmpC Riboregulator RBS C1 tarna Ribosome Negative Feed Back cr Loop RBS coding sequence
6 Red Light-Dark Sensor Red Light Input Dark Sensor pcya ho1 cph8 Receptor Phococyanobilin
7 Negative Feed Back Output C1 GFP tarna OmpC Bingding site terminator OmpC terminator C1 R OmpC Riboregulator RBS C1 terminator tarna Ribosome Negative Feed Back cr Loop RBS coding sequence
8 Red Light Input Dark Sensor pcya ho1 cph8 Output C1 operon GFP tarna OmpCC1 operon terminator OmpC terminator C1 R OmpC Riboregulator RBS C1 tarna Ribosome Negative Feed Back cr Loop RBS coding sequence
9 MAIN CIRCUIT CRITICALITY DETECTOR
10 For the Main Circuit RESULTS J23100 cph8 ho1 pcya Terminator C1 GFP R C1 tarna ompc Binding site Terminator riboregulator Terminator Terminator
11 MODELLING
12
13
14 APPLICATION TARGETED ANTIMICROBIAL PEPTIDES! Bac8c & AAP2 S. mutans & Dental caries
15 Bac8c & AAP2 Pulse Generator Dosage Control Antimicrobial Peptide Stable Powerful Homeostasis Maintenance S.mutans & Dental caries Streptococcus. mutans Common in Oral Cavity Easy to Overgrowth Main Cause of Dental Caries
16 APPLICATION Streptococcus.mutans Input : population of S.mutans, implying by the ph of the micro-environment kill and inhibit Criticality! detector including a AsR promoter CAP GGG AAP2 Bac8c Red Light-Dark Sensor Low ph Sensor AsR Promoter to Replace
17 EXPERIMENTS For the Application CAP GGG APP2 APP2 promotor Terminator CAP GGG Bac8c Bac8c promotor Terminator Function Tests: pet28a in E. coli BL21
18 RESULTS OD600 in 722s For the Application
19
20 RESULTS A For the Application Kirby Bauer Antibiotic Testing E B A.AAP2 (ph=5) B.Bac8c (ph=7) D.AAP2 (ph=7) D C C.0.5% Kanamycin(Left) Sterile Water (Right) E.CAP-Bac8c (ph=7)
21 ONE NEW TRICK Short DNA Segment Insertion CI binding site Primer 1 tarna Riboregulator Production (Linear blunt-end DNA) The first pair of primers open the circular plasmid at a specific cite.
22 ONE NEW TRICK Primer 2 Production (DNA with complementary ends) The second pair of primers conduct insertion and back-splicing.
23 ONE NEW TRICK Short DNA Fragment Insertion C1 GFP R C1 tarna Binding site riboregulator Terminator Terminator Terminator
24 FUTURE WORK Engineering in S. mutans Binary System and Biocomputing recombinases 2nd 2nd Int Output Input Xis Int 1 0
25 HUMAN PRACTICE Sketch in Dish Smile Mates
26 Sketch in Dish Primary students and parents Painter: E.coli Canvas: Agar dish Education A little show of sketches Basic transgenetic knowledge Interesting activity Curiosity and A Early Star
27 Smile Mates Free and Professional Oral Condition Examnation Beyond pure medical science Massive kindness and trusts Public Engagement Dental caries prevention Huge Attentions Creative Responsibilities and Trust
28
29 ACKNOWLEDGEMENTS Meet Up 2 Wuhan meet up & CCIC in Beijing Help Received Help Sent Out CI Operon from HUST Bxb1 Recombinases from ZJU JLU; Newsletter for Amoy
30 ACHIEVEMENTS Criticality detector: Plasmids 2,successful modelling. Antimicrobial peptides: Plasmids 4, function tested. Original trick for short DNA fragment insertion. Two large scale activities outside lab. Wuhan meet up.
31 ACKNOWLEDGEMENTS
32 LIMBO CRITICALITY DETECTION WHU-CHINA 2015
33 THANKS
34 LIMBO CRITICALITY DETECTION WHU-CHINA 2015
35 Sketch in Dish
36 MODELLING
37 Binary System and Biocomputing
38 Red Light-Dark Sensor Red Light Input cell membrane * Cph1 EnvZ Cph8* haem OpmR regulator phycocyanobilin pcya ho1 cph8 BBa_J23100 ompc
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