ISTA Annual Meeting, Zürich June 14, 2011

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2 FTSC MEMBERS Chair Fabio Gorian Italy GER, STO, BSC Vice-chair Zdenka Prochazkova Czech Republic Members Isolde D. Kossmann Ferraz Brazil Robert P. Karrfalt United States MOI Dave Kolotelo Canada Stefanie Krämer Germany TEZ Beti Piotto Italy Hugh W. Pritchard United Kingdom STO Heidi Røsok Bye Dale Simpson Norway Canada Victor Vankus United States AOSA New Rosa Colomer Spain New Efisio Mattana Italy (New Czeslaw Koziol Poland)

3 1. Abies stratification 2. Water activity measurement 3. Alternative germination substrate for conservation programs and small lots 4. MOI: working sample

4 1. Abies stratification 2. Water activity measurement 3. Alternative germination substrate fpretreatments on Mediterranean shrub seedsor conservation programs and small lots 4. MOI: working sample Abies alba Mill. This item will be deleted from the FTS programme: 1. Time consuming method 2. Useful only with few Abies species 3. No advantages compared with other methods

5 1. Abies stratification 2. Water activity measurement 3. Alternative germination substrate for conservation programs and small lots 4. MOI: working sample This item is supported by Robert Karrfalt FTS-MOI member: 1. Alternative method to the moisture content determination 2. Data to compare the two tests are needed 3. Rules proposal in progress in Chapter 9 4. Validation test among laboratories

6 1. Abies stratification 2. Water activity measurement 3. Alternative germination substrate for conservation programs and small lots 4. MOI: working sample The use of the agar for germination tests in conservation programs and with small lots 1. sterile substrate; 2. Homogeneous and constant moisture conditions

7 1. Abies stratification 2. Water activity measurement 3. Alternative germination substrate for conservation programs and small lots 4. MOI: working sample Discussion is open among MOI members to change ISTA rule n (and ): Proposal: also for large seeds (as tree seeds) 2 working samples each of 5 gr

8 A- Rules development A1 Introduction of new methods Information from MRI (X ISSS in Bahia-Brazil) Double embryos (Beech) Empty seed (Fir)

9 Viable vs non-viable Fagus sylvatica L. seeds Viable Non-viable Evidence of internal structure A- Rules development A1 Introduction of new methods No evidence of internal structure Dead embryo

10 A- Rules development A1 Introduction of new methods Viable Fagus sylvatica L. seeds

11 A- Rules development A1 Introduction of new methods Non-viable Fagus sylvatica L. seeds

12 1. Validation of germination methods for tropical/subtropical species 2. Tetrazolium 3. Pretreatments on Mediterranean shrub seeds 4. Juglans spp.

13 1. Validation of germination methods for tropical/subtropical species 2. Tetrazolium 3. Pretreatments on Mediterranean shrub seeds 4. Juglans spp. Basic germination protocols for many tropical tree species are available. Validation method of J.curcas: difficult to recovery fresh seed

14 1. Validation of germination methods for tropical/subtropical species 2. Tetrazolium 3. Pretreatments on Mediterranean shrub seeds 4. Juglans spp. TETRAZOLIUM OF SOME TREE AND SHRUBS SPECIES* *Tetrazolium testing Vol.II-Supplements 2011 to 1 edition 2003 SPECIES FAMILY PRETREATMENT PREPARATION Betula spp. Betulaceae Water 18 hours Cut off 1/3 Chamaedorea elegans Arecaceae Water 36 hours Remove pericarp Chimonanthus spp. Calycanthaceae Water 18 hours Cut off 1/3 Livistona spp. Arecaceae Water 18 hours Remove pericarp Musa spp. Musaceae Water 18 hours Cut off a small piece Pachypodium spp. Apocynaceae Water 18 hours Remove seed coat Panax gingseng Araliaceae Water 18 hours Cut off 1/4

15 1. Validation of germination methods for tropical/subtropical species 2. Tetrazoilum 3. Pretreatments on Mediterranean shrub seeds 4. Juglans spp. GERMINATION % OF SOME MEDITERRANEAN TREE AND SHRUBS SPECIES* *Montaraz laboratories -Madrid (Spain) SPECIES TREATMENT % GER DAYS Acer monspessulanum Cold stratification (3,5-4 months) Arbutus unedo Cold stratification (1 month) Atriplex halimus No treatment 30 7 Ceratonia siliqua Sulfuric acid (1 hour) Cistus clusii Boiling water (1 minute) Cytisus grandiflorus No treatment Lygeum spertium No treatment 70 21

16 1. Validation of germination methods for tropical/subtropical species 2. Tetrazoilum 3. Pretreatments on Mediterranean shrub seeds 4. Juglans spp. GERMINATION % OF SOME MEDITERRANEAN TREE AND SHRUBS SPECIES* *Montaraz laboratories -Madrid (Spain) SPECIES TREATMENT % GER DAYS Pistacia terebinthus Cold stratification (2 months) Retama sphaerocarpa Sulfuric acid (4 hours) Stipa tenacissima No treatment Tetraclinis articulata No treatment Thymus zygis No treatment Ulex parviflora Boiling water (5 seconds) Vitex agnus-castus Cold stratification (6 months) 30 21

17 1. Validation of germination methods for tropical/subtropical species 2. Tetrazoilum 3. Pretreatments on Mediterranean shrub seeds 4. Juglans spp. GERMINATION % OF SOME MEDITERRANEAN TREE AND SHRUBS SPECIES* *Banca del germoplasma della Sardegna-Centro Conservazione Biodiversità Cagliari (Italy) SPECIES TREATMENT % GER DAYS Juniperus oxycedrus ssp. macrocarpa Juniperus phoenicea ssp.turbinata No treatment No treatment Pistacia lentiscus No treatment Rhamnus persicifolia Ribes multiforum ssp.sandalioticum Cold stratification (3 months) Warm stratification (3 months) 90 40

18 1. Validation of germination methods for tropical/subtropical species 2. Tetrazolium 3. Pretreatments on Mediterranean shrub seeds 4. Juglans spp. Why? 1. Both ISTA and AOSA no rules 2. Important commercial species How? 1. Germination (prechilling for 3 months_then C) 2. Tetrazolium (Harmonization with AOSA handbook) Viable No viable

19 1. Tree and shrub seed handbook (revision) 2. Sampling handbook (tree seed)

20 1. Tree and shrub seed handbook (revision) 2. Sampling handbook (tree seed) Dale Simpson (Canada), Bye Heidi Resok (Norway), Dave Kolotelo (Canada), Beti Piotto (Italy) and Zdenka Prochazkova (Czech Republic) are involved in the revision of the ISTA Tree and shrub seed handbook. On the basis of a first draft, the group is at the moment discussing the format and contents. Concerns regard production and publication costs even if an on line version could be assumed.

21 1. Tree and shrub seed handbook (revision) 2. Seed sampling handbook (tree seed) The FTS Committee revised in 2011 the handbook (Chapters 2, 4, 5 and 6) Discussion is open among the BSC members

22 1. Guia de propagulos e plantulas da Amazonia, vol.1 (English version) 2. Guia de propagulos e plantulas da Amazonia, vol.2 3. Handbook on seedling evaluation(tree seed)

23 1. Guia de propagulos e plantulas da Amazonia, vol.1 (English version) 2. Guia de propagulos e plantulas da Amazonia, vol.2 3. Handbook on seedling evaluation(tree seed) Isolde Ferraz is looking for funds to print the English version of Vol 1 and Portuguese version of Vol.2 (unpublished)

24 1. Guia de propagulos e plantulas da Amazonia, vol.1 (English version) 2. Guia de propagulos e plantulas da Amazonia, vol.2 3. Handbook on seedling evaluation(tree seed) Ex. Adenocarpus complicatus Abnormal Normal The handbook Seedling evaluation needs some standardization regarding the pictures

25 1) Effect of different storage conditions in recalcitrant seeds of holm oak (Quercus ilex L.) during germination Pasquini, S., Braidot, E., Petrussa, E. and Vianello, A. (2011), Seed Sci. & Technol., 39, ) Seed germination of a recalcitrant species (Quercus ilex L.) stored in polyethylene bags: analysis of some bioenergetic and oxidative parameters Pasquini, S., Mizzau, M., Petrussa, E., Braidot, E., Gorian, F., Lambardi, M., Vianello, A.. Under revision

26 1. Taipei (Taiwan): Recent Advances in Seed Research and ex situ Conservation (August 2010) 2. Montargis (F): Workshop on Water activity (Oct.2010) 3. Madrid: Workshop on Mediterranean species (2013)

27 IUFRO Seed Physiology and Technology Taipei, August 15 21, 2010 Recent Advances in Seed Research and ex situ Conservation Ex situ conservation issues associated with recalcitrant seeds (H. W. Pritchard) Ex situ conservation issues relevant to the ISTA (Z. Prochazkova) Proceedings in pdf available

28 1. Montargis(F): Workshop on Water activity (Oct.2010) 2. Madrid: Workshop on Mediterranean species (2013) 20 Participants 6 lecturers

29 1. Montargis(F): Workshop on Water activity (Oct.2010) 2. Madrid: Workshop on Mediterranean species (2013) Three days workshop 5 sessions (among them Water activity and equilibrium relative humidity concepts; Operational applicability of Water activity measurement; Water activity measurement applied to ex situ conservation of genetic resources ) Practical work was done with aw meters on several seed and pollen species including forest and agricultural seeds.

30 1. Montargis (F): Workshop on Water activity (Oct.2010) 2. Madrid: Workshop on Mediterranean species (2013) Dormancy breaking and treataments with mediterranean species Workshop organised by Semillas Montaraz in Madrid Springtime 2013

31 1. Revision of the definition of the qualified category 2. Development of common definitions 3. Draft guidelines on the FRM chain 4. Short rotation plantation for bioenergy and agro-forestry and shrub species 5. Proposal on how to deal with the category Tested

32 2-Paris, October 13-15, 2011 OECD- Forest Scheme Annual Meeting

33 1. Verona (I): OECD Forest scheme 2012 annual meeting or 2012 TWG annual meeting?

34 1. Pinus sylvestris (I): in collaboration with Pur and Ger Committees to be finalised in 2013

35 Thank you Steve!