Lunch. Hillarpsalen. Lunch. KLR and HM. Hillarpsalen. Cancer. ET Hillarpsalen
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1 Study time 1. Medical genetics, april Week April Introduction JL Chromosomes and Chromosomal abnormalities PG Microdeletion and duplication syndromes JL 12 April Monogenic and complex disorders KLR and HM Monogenic and complex disorders KLR and HM 13 April Prenatal diagnosis and genetic counselling EB Cancer ET 14 April - study questions - preparation for exercises 15 April Exercises in medical genetics * Betacellen(L1:00), Studiecenter(L4:00), Goliat(L2:03), David(L2:03), Simson(L2:03) Mandatory - preparation for exercises JL Johanna Lundin KLR Kristina Lagerstedt Robinson PG Peter Gustavsson ET Emma Tham HM Helena Malmgren EB Erik Björck JL Johanna Lundin, Retzius väg 8, Karolinska Institutet, Solna 1
2 2. Molecular biological techniques, April 18- May 6 Week April Introduction PA/CZ Methods to analyse DNA HG Enzymes used in DNA manipulation TJ Transfection and reporter genes CZ 19 April Cloning techniques and PCR TJ Real time PCR TJ Global gene expression FF New generation sequencing PM Immunohistochemistry KY 20 April Protein:protein interactions CZ Introductory Microbiology: Understanding Our Microbiome VA Epigenetics AL Introduction to the lab* AL 21 April RNA extraction Analyzing epigenetic data IS DNA-binding / promoter recruitment, chromatin JR RNA extraction 22 April cdna synthesis and PCR cdna synthesis and PCR Alfred Nobels Allé 8, Lecture hall ;; Hospital, Hus F, Blickagången 9, Kurslab 2 PS Peter Swoboda HG Hui Gao Carsten Daub AL Andreas Lennartsson IS Indranil Sinha FF Fredrik Fagerström-Billai CZ Chunyan Zhao PA Per Antonson PM Patrick Müller 2
3 Molecular biological techniques, continuing Week April Isolation of DNA fragments and restriction enzyme digestion and DNA cloning Isolation of DNA fragments and restriction enzyme digestion and DNA cloning 26 April Transformation Transformation 27 April Transformation Protein analysis (1D/2D Western, Massspec, proteomics), I IN 6F Protein analysis (1D/2D Western, Massspec, proteomics) II Transformation IN 6F 28 April Transgene technology Knockout technology PA PA CRISP/CAS9 TJ Fluorescence microscopy SLG Lab: * Start o/n cultures Lab: * Start o/n cultures 29 Aprl Restriction enzym digest and gel analysis Time for questions Restriction enzym digest and gel analysis Alfred Nobels Allé 8, Lecture hall and 6F;; Hospita, Hus F, Blickagången 9, Kurslab 2 PS HG Carsten Daub AL Andreas Lennartsson IS FF Fredrik Fagerström-Billai CZ Chunyan Zhao PA Per Antonson PM Peter Swoboda Hui Gao Indranil Sinha Patrick Müller 3
4 Molecular biological techniques, continuing Week May /write labreport 3 May /write labreport 4 May Intermediate exam 2 /write labreport Exam hall 5Y 5 May Kristi Himmelfärdsdag 6 May Alfred Nobels Allé 8, Lecture hall, 6F and Exam hall 5Y, Hospita, Hus F, Blickagången 9, PS Peter Swoboda HG Hui Gao Carsten Daub AL Andreas Lennartsson IS Indranil Sinha FF Fredrik Fagerström-Billai CZ Chunyan Zhao PA Per Antonson PM Patrick Müller 4
5 3. Bioinformatics, May Week May Introduction to Bioinformatics Scientific publications and archives Lab 1 * 10 May Genome organization and evolution Archives and information retrieval Lab 1 * 11 May Alignments and phylogenetic trees Structural bioinformatics Lab 2 * 12 May Proteomics and systems biology? Lab 2* 13 May C. Elegans PS Alfred Nobels Allé 8, Lecture hall, 6F and Computer room Matilda, 6G PS HG Carsten Daub AL Andreas Lennartsson IS FF Fredrik Fagerström-Billai CZ Chunyan Zhao PA Per Antonson PM Peter Swoboda Hui Gao Indranil Sinha Patrick Müller 5
6 3. Structure biology, May Week May Protein structure and stability 17 May Methods to determine three-dimensional structure I 18 May Methods to determine three-dimensional structure II 19 May Other techniques to determine protein structure 20 May Structure prediction and molecular modelling Correction, Group 1-8 Alfred Nobels Allé 8, Lecture hall, 6F and Computer room Matilda 6G PS HG Carsten Daub AL Andreas Lennartsson IS FF Fredrik Fagerström-Billai CZ Chunyan Zhao PA Per Antonson PM Peter Swoboda Hui Gao Indranil Sinha Patrick Müller 6
7 4. Strukturbiologi, 19 maj - 23 maj Week May Retake Intermediate exam 2 Preparation of presentations Seminar room RED/BLUE 24 May Preparation of presentations 25 May Preparation of presentations 26 May Integrating project * Presentations Integrating project * Presentations 27 May Alfred Nobels Allé 8, Lecture hall Seminar room GREEN,Novum, main building, floor 6 elevator F PS Peter Swoboda HG Hui Gao Carsten Daub AL Andreas Lennartsson IS Indranil Sinha FF Fredrik Fagerström-Billai CZ Chunyan Zhao PA Per Antonson PM Patrick Müller 7
8 Week May Time for questions Course council meeting Online chat GREEN Meeting Room 31 May 1 June 2 June 09:00-15:00 Exam Exam hall 5Y 3 June Alfred Nobels Allé 8, Lecture hall and Exam hall 5Y;; Novum, Blickagången 6, floor 6 (elevator F), Meeting room GREEN, PS Peter Swoboda HG Hui Gao Carsten Daub AL Andreas Lennartsson IS Indranil Sinha FF Fredrik Fagerström-Billai CZ Chunyan Zhao PA Per Antonson PM Patrick Müller 8
9 Adresser till undervisningslokaler All maps over Karolinska, Solna and Huddinge are available on the course website under the heading "Schedule". sv/publicpage.do?item= Lecture hall Retzius väg 8 KI Campus, Solna Lecture hall Alfred Nobels Allé 8, floor 6 KI Campus, Huddinge Course lab 2 Hus F2, plan 8 Karolinska Universitetssjukhuset, Huddinge Literature Bioinformatics Arthur Lesk, Introduction to Bioinformatics, 3rd edition 1: Introduction 2: Genome organization and evolution 3: Scientific publications and archives 4: Archives and information retrieval 5: Alignments and phylogenetic trees 6: Structural bioinformatics 7: Proteomics and systems biology Computer room 6G Alfred Nobels Allé 8, floor 6 KI Campus, Huddinge Exam hall 5Y Alfred Nobels Allé 8, floor 5 KI Campus, Huddinge Seminar room GREEN Novum, Blickagången 6, elevator F, floor 6, KI Campus, Huddinge 9
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