Tools for Assessing Cell Events Apoptosis, Cell Cycle and Cell Proliferation Product List

Transcription

1 BD Biosciences Tools for Assessing Cell Events Apoptosis, Cell Cycle and Cell Proliferation Product List For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2 Abbreviations The following are common abbreviations that might appear throughout the Apoptosis Reagents Product List. APPS Applications IF Immunofluorescence Ar Armenian hamster IHC Immunohistochemistry Bab BD IMag - DM Bioimg Block Bov Chick Ctrl Cost Cyno EA ELISA ELISA Cap. ELISA Det. ELISPOT FA FCM FCM* Baboon Direct Magnet Bioimaging Blocking Bovine Chicken Controls Co-stimulation Cynomolgus Enzyme assay Enzyme-linked immunosorbent assay ELISA Capture ELISA Detection Enzyme-linked immunospot Functional assay Flow cytometry Flow cytometry. Suitable for use with intracellular staining protocols using BD Cytofix/Cytoperm. IHC(F)* IHC(Fr) IHC(P) IHC(Zn) IP IP/WB ISH Lym Mam Monk Ms NA/LE Neu NHP PE Pg Pig Immunohistochemistry formalin (antigen retrieval required) Immunohistochemistry frozen Immunohistochemistry formalin fixed, paraffin Immunohistochemistry zinc fixed Immunoprecipitation Immunoprecipitation/Western blot In situ hybridization Lymphocyte Mammalian Monkey Mouse No azide, low endotoxin Neutralization Non-human primate Phycoerythrin Pigeon Porcine FCM/IA Flow cytometry/immunoassay Prim Primate FITC Fluorescein isothiocyanate Qua Quail G Pig Guinea pig Rab Rabbit GMP Good Manufacturing Practices REACT Reactivity Gt Goat Rhe Rhesus Ham HRP Hs Hu IC ICtrl IC Ctrl ICC Hamster Horseradish peroxidase Horse Human Intracellular staining Isotype control Intracellular control Immunocytochemistry (cytospins) RUO Sep Syr Ham Tr Vir WB Xen For Research Use Only. Not for use in diagnostic or therapeutic procedures. Cell separation Syrian hamster Trout Viral Western blot Xenopus IC/FCM Intracellular staining (flow cytometry) 2 bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.

3 Life, Death, and Cell Proliferation The balance of cell proliferation and apoptosis is important for both development and normal tissue homeostasis. Cell proliferation is an increase in the number of cells as a result of growth and division. Cell proliferation is regulated by the cell cycle, which is divided into a series of phases. Apoptosis or programmed cell death results in controlled self-destruction. Several methods have been developed to assess apoptosis, cell cycle, and proliferation. BD Biosciences offers a complete portfolio of reagents and tools to allow exploration of the cellular features of these processes. Apoptosis Application Decision Tree Apoptotic Cells Intact Cells Cell Extracts Tissue Sections Flow Cytometry Bioimaging/Microscopy ELISA IHC Live Fixed Live Fixed Active Caspase-3 Cleaved PARP Active Caspase-3 Cleaved PARP Loss of Membrane Asymmetry Annexin V Cleaved Markers Active Caspase-3 Cleaved PARP Annexin V Active Caspase-3 Cleaved PARP Western Blot or Immunoprecipitation Mitochondrial Membrane Potential MitoScreen (JC-1) TUNEL/DNA Fragmentation APO-DIRECT APO-BRDU Apoptosis Markers Spectrofluorometry Caspase Activity All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. 3 bdbiosciences.com

4 Apoptosis Product List Kits/Sets/Pairs description react clone apps reg contents size cat. no. Annexin V FITC Fluorescence Microscopy Kit IF RUO Annexin V-FITC, 25 Slides Annexin V Binding Buffer, and PBS Buffer Annexin V FITC Apoptosis Detection Kit I FCM RUO Annexin V-FITC, 100 tests Propidium Iodide Staining Solution, Annexin V Binding Buffer Annexin V FITC Apoptosis Detection Kit II FCM RUO Purified Recombinant 100 tests Annexin V, Annexin V-FITC, Propidium Iodide Staining Solution, Annexin V Binding Buffer Annexin V PE Apoptosis Detection Kit I FCM RUO Annexin V-PE, 7-AAD, 100 tests and Annexin V Binding Buffer Apoptosis Detection Kit (APO-BRDU) IC/FCM RUO FITC-Labeled Anti-BrdU 60 tests mab, PI/RNase Staining Buffer, Reaction Buffer, Rinsing Buffer, Wash Buffer, Br-dUTP, TdT Enzyme, Negative Control Cells, Positive Control Cells Apoptosis, DNA Damage, and Cell Proliferation Kit IC/FCM RUO BrdU PerCP-Cy 5.5, 50 Tests H2AX (ps139) Alexa Fluor 647, Cleaved PARP (Asp214) PE; DAPI; BrdU solution; DNase; BD Cytofix/Cytoperm Fixation/Permeabilization Solution; BD Perm/Wash Buffer Apoptosis Detection Kit (APO-DIRECT) IC/FCM RUO FITC-dUTP, PI/RNase 50 tests Staining Buffer, Reaction Buffer, Rinsing Buffer, Wash Buffer, TdT Enzyme, Negative Control Cells, Positive Control Cells Apoptosis I Sampler Kit IF, WB RUO Apaf-1, Bad, Bax, Bcl-2, 10 μg each Bcl-x, BRUCE, CAS, hilp/xiap, Nip1, p53 Apoptosis II Sampler Kit IF, WB RUO Caspase-2/ICH-1L, 10 μg each Caspase-3/CPP32, Caspase-7/MCH-3, DFF45, FADD, Fas/CD95/APO 1, PARP, RIP, TRADD Apoptotic Jurkat Lysate Set I WB RUO Jurkat Control Lysate 500 μg each and Camptothecin-treated Jurkat Lysate Bcl-2 Related Sampler Kit IF, WB RUO Bad, BAG-1, Bax, Bcl-2, 10 μg each Bcl-x, Bcl-x, Beclin, Bid BrdU APC Flow Kit IC/FCM RUO 50 tests IC/FCM RUO 4 x 50 tests BrdU FITC Flow Kit IC/FCM RUO 50 tests IC/FCM RUO 4 x 50 tests BrdU In-Situ Detection Kit IHC(Zn), IHC(F), IHC(Fr) RUO Fixation Buffer, Diluent 50 tests Buffer, BD Retrievagen A Antigen Retrieval Solution, biotinylated anti-brdu antibody, ready-to-use Streptavidin-Horseradish peroxidase (SAv-HRP) Solution, DAB Buffer, DAB Chromogen, and Control Slides BrdU In-Situ Detection Kit II IHC(Zn), IHC(F), IHC(Fr) RUO Fixation Buffer, Diluent 200 tests Buffer, BD Retrievagen A antigen retrieval solution, biotinylated anti-brdu antibody, ready-to-use Streptavidin-Horseradish peroxidase (SAv-HRP) solution, DAB Buffer, DAB Chromogen, and Control Slides Caspase-3 Assay Kit SF, FA RUO PBS Buffer, Cell Lysis 100 tests Buffer, HEPES Buffer, Caspase-3 Fluorogenic Substrate, Caspase-3 Inhibitor 4 bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.

5 Kits/Sets/Pairs description react clone isotype apps reg contents size cat. no. Caspase-3, Active Hu C92-605, 19 Rab IgG, Mouse IgG 2a ELISA RUO Purified Reagents for Form, ELISA Pair 10 plates Caspase-3, Active FA, SF RUO Set 20 tests Form, Enzyme Set Caspase-3, Active Form, Hu, Ms C Rab IgG IC/FCM RUO FITC-conjugated 100 tests mab Apoptosis Kit : FITC Monoclonal Rabbit Anti-Active Caspase-3 Antibody, BD Cytofix/Cytoperm Fixation/Permeabilization Solution, and BD Perm/Wash Buffer Caspase-3, Active Form, Hu, Ms C Rab IgG IC/FCM RUO PE-conjugated 100 tests mab Apoptosis Kit : PE Monoclonal Rabbit Anti-Active Caspase-3 Antibody, BD Cytofix/Cytoperm Fixation/Permeabilization Solution, and BD Perm/Wash Buffer Cell Cycle Kit Bioimg RUO 96 assays Chaperone Sampler Kit IF, WB RUO BiP/GRP78, Calnexin, 10 μg each ERp72, HSF4, Hsp70, Hsp90, Hsp110, PDI, STI1/Hop-p60 BD Cycletest Plus FCM RUO(GMP) Solution A: trypsin in 40 tests DNA Reagent Kit spermine tetrahydrochloride detergent buffer; Solution B: RNase A and trypsin inhibitor in spermine buffer; Solution C: Propidium Iodide (PI) in spermine buffer; Buffer Solution: dimethylsulfoxide (DMSO) in sucrose-sodium citrate Human CD253 ELISA Set Hu ELISA RUO Reagents for plates Human LT-a ELISA Set Hu ELISA RUO Reagents for plates Human TNF ELISA Kit II Hu ELISA RUO 2 plates Human TNF ELISA Set Hu ELISA RUO Reagents for plates Mouse TNF (Mono/Mono) Ms ELISA RUO Reagents for ELISA Set 20 plates Mouse TNF (Mono/Mono) Ms ELISA RUO Reagents for ELISA Set II 20 plates Mouse TNF (Mono/Poly) Ms ELISA RUO 2 plates ELISA Kit II Mouse TNF ELISA Kit Ms ELISA RUO 2 plates Rat TNF ELISA Kit Rat ELISA RUO 2 plates Rat TNF ELISA Set Rat ELISA RUO Reagents for plates Human Granzyme B Hu ELISPOT RUO 10 plates ELISPOT Set Human TNF ELISPOT Pair Hu ELISPOT RUO 5 plates Human TNF ELISPOT Set Hu ELISPOT RUO 10 plates Mouse TNF ELISPOT Pair Ms ELISPOT RUO 5 plates Mouse TNF ELISPOT Set Ms ELISPOT RUO 10 plates BD MitoScreen (JC-1) IC/FCM RUO JC-1 Dye 100 tests and Assay Buffer All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. 5 bdbiosciences.com

6 Apoptosis Product List CBA Reagents description react apps reg size cat. no. Human Basic FGF Hu FCM RUO 100 tests Flex Set (Bead C5) Human CD178 (Fas Hu FCM RUO 100 tests Ligand) Flex Set (Bead C6) Human Granzyme A Hu FCM RUO 100 tests Flex Set (Bead D9) Human Granzyme B Hu FCM RUO 100 tests Flex Set (Bead D7) Human LT-a Flex Set Hu FCM RUO 100 tests (Bead D5) Human TNF Flex Set Hu, NHP FCM RUO 100 tests (Bead D9) Human TNF Flex Set Hu FCM RUO 100 tests (Bead C4) Human TNF Enhanced Hu FCM RUO 100 tests Sensitivity Flex Set (Bead C4) Mouse TNF Flex Set Ms FCM RUO 100 tests (Bead C8) Rat TNF Flex Set Rat FCM RUO 100 tests (Bead C8) Human Soluble Protein Hu FCM RUO 100 tests Master Buffer Kit FCM RUO 500 tests Mouse/Rat Soluble Ms, Rat FCM RUO 100 tests Protein Master Buffer Kit FCM RUO 500 tests Apoptosis Product List Apoptosis Reagents A20 Hu E Ms IgG 1, l WB RUO Purified 50 µg AIF (Apoptosis-inducing Ms, Rat, Hu Polyclonal Rab Ig WB RUO Purified 100 µg factor) AIP1 Ms, Rat 49 Ms IgG 1 WB RUO Purified 50 µg WB RUO Purified 150 µg ALDH Hu 44 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Annexin V FCM RUO Purified 100 µg FCM RUO Biotin 100 tests FCM RUO Biotin 200 tests FCM RUO APC 25 tests FCM RUO APC 100 tests FCM RUO APC 200 tests FCM RUO Cy tests FCM RUO Cy5 200 tests FCM RUO Cy tests FCM RUO FITC 25 tests FCM RUO FITC 100 tests FCM RUO FITC 200 tests FCM RUO BD Horizon V tests FCM RUO BD Horizon V tests FCM RUO PE 25 tests FCM RUO PE 100 tests FCM RUO PE 200 tests AOX1 Hu, Ms, Rat 7 Ms IgG 1 WB RUO Purified 50 µg bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.

7 Apoptosis Reagents Apaf-1 Hu 24 Ms IgG 1 WB, IF RUO Purified 150 µg WB, IF RUO Purified 50 µg Hu, Ms Polyclonal Rab Ig WB RUO Purified 50 µg Bad Hu, Ms, Rat 48 Ms IgG 2b IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg IF RUO FITC 50 µg IF RUO FITC 150 µg IF RUO TRITC 50 µg IF RUO TRITC 150 µg BAG-1 Hu 19 Ms IgG 1 WB RUO Purified 50 µg Bak Hu G317-2 Ms IgG 1 WB RUO Purified 0.1 mg Polyclonal Rab Ig WB RUO Serum 0.1 ml basic FGF Chick, Dog, Hu, Ms, Rat 3 Ms IgG 1 IF, WB RUO Purified 150 µg Ms IgG 1 IF, IHC, IP, WB RUO Purified 50 µg IF, IHC, IP, WB RUO Purified 150 µg Bax Dog, Hu 3 Ms IgG 1 IF, IP, WB RUO Purified 50 µg IF, IP, WB RUO Purified 150 µg Hu, Ms, Rat 6A7 Ms IgG 1, k IP, WB RUO Purified 0.1 mg Hu Polyclonal Rab Ig WB RUO Serum 0.1 ml Ms, Rat Polyclonal Rab Ig IHC(F), IHC(Fr), IP, WB RUO Serum 0.1 ml Bcl-2 Ms 3F11 Ar Ham IgG1 FCM, IF, IHC(Fr), IP, WB RUO Purified 0.1 mg FCM RUO FITC Set 100 tests FCM RUO PE Set 100 tests Hu 6C8 Ar Ham IgG2, k WB RUO Purified 50 µg WB RUO Purified 150 µg FCM RUO FITC Set 100 tests FCM RUO PE Set 100 tests Ms IgG 1 IC/FCM, IHC(Fr), WB, IHC(P) RUO Purified 50 µg IC/FCM, IHC(Fr), WB, IHC(P) RUO Purified 150 µg FCM, IC RUO(GMP) FITC 50 tests IC/FCM RUO FITC Set 100 tests IC/FCM RUO BD Horizon V tests FCM, IC RUO(GMP) PE 50 tests IC/FCM RUO PE Set 100 tests D7 Ms IgG 1 WB RUO Purified 50 µg WB RUO Purified 150 µg Chick, Dog, Hu, Ms, Rat 7 Ms IgG 1 IF, IHC, IP, WB RUO Purified 50 µg IF, IHC, IP, WB RUO Purified 150 µg Ms, Rat Polyclonal Rab Ig WB RUO Serum 0.1 ml IHC(F), IHC(Fr), IP, WB RUO Serum 0.1 ml Bcl-2 (ps70) Hu N Ms IgG 1 WB, IHC(F)*, IC/FCM RUO Purified 0.1 mg IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests Bcl-10 Hu 151 Ms IgG 1, k FCM RUO PE Set 100 tests Bcl-w Ms, Hu 16H12 Rat IgG 2a, k WB RUO Purified 50 µg Bcl-x Hu, Ms 2H12 Ms IgG 2a WB RUO Purified 50 µg WB RUO Purified 150 µg Chick, Ms, Rat 4 Ms IgG 2b IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg Hu, Ms, Rat 44 Ms IgG 1 IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg Chick, Dog, Hu, Ms, Rat Polyclonal Rab IgG IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg Hu, Ms Polyclonal Rab Ig IHC(F), IHC(Fr), WB RUO Serum 0.1 ml Bcl-xL/S Hu, Ms Polyclonal Rab Ig IHC(F), IHC(Fr), IHC(P), WB RUO Serum 0.1 ml All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. 7 bdbiosciences.com

8 Apoptosis Product List Apoptosis Reagents Beclin Ms, Rat, Hu, Dog, Chick 20 Ms IgG 2a WB RUO Purified 50 µg WB RUO Purified 150 µg Bid Hu 7 Ms IgG 1 IF, WB RUO Purified 50 µg Ms 40 Ms IgG 1 WB RUO Purified 50 µg Bik Hu C33-1 Ms IgG 1, k WB RUO Purified 0.1 mg Bim Hu, Ms, Rat Polyclonal Rab Ig WB RUO Purified 50 µg Blys Hu H Ms IgG 1, k ELISA Cap. RUO Purified 0.1 mg H Ms IgG 1, k ELISA Det. RUO Biotin 0.1 mg BrdU Hu 3D4 Ms IgG 1, k IHC(Fr), IC/FCM RUO Purified 0.1 mg Bioimg RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests FCM, IC/FCM RUO FITC Set 100 tests FCM, IC/FCM RUO PE Set 100 tests Hu, Ms 3D4 Ms IgG 1, k IC/FCM RUO BD Horizon V µg IC/FCM RUO PerCP 50 tests Hu, Ms, Rat 3D4 Ms IgG 1, k IC/FCM RUO PerCP-Cy tests Hu B44 Ms IgG 1, k FCM RUO(GMP) Purified 100 tests FCM RUO(GMP) FITC 100 tests BRUCE Dog, Hu, Ms, Rat 4 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Btf Hu 5 Ms IgG 1 IF, WB RUO Purified 50 µg CAS Dog, Frog, Hu, Ms, Rat 24 Ms IgG 1 IF, IP, WB RUO Purified 50 µg Caspase-2 (Ich-1L) Hu 35 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg G Ms IgG 1 Bioimg, WB RUO Purified 50 µg Caspase-3 Hu 19 Ms IgG 2a IF, IP, WB RUO Purified 50 µg IF, IP, WB RUO Purified 150 µg Ms 46 Ms IgG 1 IF, IP, WB RUO Purified 50 µg IF, IP, WB RUO Purified 150 µg Caspase-3, Active Form Hu C Rab IgG IC/FCM RUO Alexa Fluor tests IC/FCM RUO FITC 25 tests IC/FCM RUO BD Horizon V tests IC/FCM RUO PE 25 tests IC/FCM RUO PE 100 tests Hu, Ms C Rab IgG IHC(Fr), IP RUO Purified 25 µg IC/FCM RUO Biotin 100 tests IC/FCM RUO FITC 100 tests Polyclonal Rab IgG IHC(Fr) RUO Biotin 0.1 mg IC/FCM RUO PE 100 tests Caspase-4 Hu, Ms, Rat B25-1 Ms IgG 1, k WB RUO Purified 0.1 mg Caspase-6 (Mch2a) Hu B93-4 Ms IgG 1, k WB RUO Purified 0.1 mg Caspase-7 Hu, Ms Ms IgG 1, k IP, WB RUO Purified 50 µg IP, WB RUO Purified 150 µg Dog, Hu 51 Ms IgG 2b IF, WB RUO Purified 50 µg Hu Ms IgG 1, k IP, WB RUO Purified 50 µg B94-1 Ms IgG 1, k IP, WB RUO Purified 0.1 mg Caspase-8 Hu Ms IgG 1, k IP, WB RUO Purified 50 µg IP, WB RUO Purified 150 µg Ms IgG 1, k WB RUO Purified 50 µg WB RUO Purified 150 µg B9-2 Ms IgG 2a, k WB RUO Purified 0.1 mg Polyclonal Rab Ig WB RUO Serum 0.1 ml WB RUO Serum 0.1 ml bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.

9 Apoptosis Reagents Caspase-9 Hu 2-22 Ms IgG 1, k WB RUO Purified 50 µg WB RUO Purified 150 µg B40 Ms IgG 1, k WB RUO Purified 0.1 mg Caspase-12 Ms, Rat, Hu Polyclonal Rab IgG WB RUO Purified 0.1 ml CD95 Chick, Dog, Hu, Ms, Rat 13 Ms IgG 1 IHC(F)*, WB RUO Purified 50 µg IHC(F)*, WB RUO Purified 150 µg Bab, Cyno, Rhe DX2 Ms IgG 1, k FCM RUO FITC 50 tests FCM RUO PE 50 tests Hu DX2 Ms IgG 1, k FCM, FA RUO NA/LE 0.5 mg FCM, IHC(Fr) RUO Purified 0.1 mg FCM RUO Biotin 100 tests FCM RUO(GMP) APC 100 tests FCM RUO APC 100 tests FCM RUO(GMP) FITC 50 tests FCM RUO FITC 100 tests FCM RUO BD Horizon PE-CF tests FCM RUO(GMP) PE 50 tests FCM RUO PE 100 tests FCM RUO PE-Cy5 100 tests EOS9.1 Ms IgM, k FCM, FA RUO Purified 0.1 mg G Ms IgG 1 WB RUO Purified 0.1 mg Ms Jo2 Ar Ham IgG2, l2 FCM RUO NA/LE 0.5 mg FCM RUO Purified 500 µg FCM RUO Biotin 0.5 mg FCM RUO FITC 0.5 mg FCM RUO BD Horizon PE-CF mg FCM RUO PE 0.2 mg FCM RUO PE-Cy mg CD120a Hu MABTNFR1-A1 Ms IgG 1, k ELISA Cap. RUO Purified 0.5 mg FCM RUO Purified 0.5 mg MABTNFR1-B1 Ms IgG 2a, k FCM RUO Biotin 0.5 mg ELISA Det., FCM RUO Biotin 0.5 mg Ms 55R-170 Ar Ham IgG1, k Block RUO NA/LE 0.25 mg R-286 Ar Ham IgG1, k ELISA Cap., FCM RUO Purified 0.5 mg CD120b Hu htnfr-m1 Rat IgG 2b, k FCM RUO Purified 0.5 mg ELISA Det., FCM RUO Biotin 0.5 mg FCM RUO PE 100 tests Ms TR75-54 Ar Ham IgG Block RUO NA/LE 0.25 mg TR75-89 Ar Ham IgG1, l3 FCM, IP/WB RUO Purified 0.5 mg FCM RUO Biotin 0.5 mg FCM RUO PE 0.2 mg CD178 Hu NOK-1 Ms IgG 1 FA, FCM RUO NA/LE 0.25 mg IP, FCM RUO Purified 0.1 mg FCM RUO Biotin 100 tests FCM RUO Biotin 0.1 mg NOK-2 Ms IgG 2a IP RUO NA/LE 0.25 mg IP RUO Purified 0.1 mg G247-4 Ms IgG 1 IHC(Fr), IP, WB RUO Purified 0.1 mg Ms Kay-10 Ms IgG 2b, k FCM, IHC(F) RUO Biotin 0.5 mg FCM RUO PE 0.2 mg MFL3 Ar Ham IgG1, k Block, FCM RUO NA/LE 0.5 mg FCM RUO Purified 0.5 mg FCM RUO Biotin 0.5 mg FCM RUO PE 0.2 mg Ms, Rat MFL4 Ar Ham IgG3, k Block, FCM RUO NA/LE 0.5 mg FCM RUO Purified 0.5 mg FCM RUO Biotin 0.5 mg All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. 9 bdbiosciences.com

10 Apoptosis Product List Apoptosis Reagents CD253 Hu B35-1 Ms IgG 2b, k WB RUO Purified 0.1 mg RIK-1 Ms IgG 1 FCM RUO Purified 0.1 mg RIK-2 Ms IgG 1 Block, FA, FCM RUO NA/LE 0.25 mg FCM RUO Purified 0.1 mg FCM RUO Biotin 0.2 mg FCM RUO PE 0.1 mg Ms N2B2 Rat IgG 2a, k FCM RUO Purified 0.1 mg CD262 Ms MD5-1 Ar Ham IgG2, k FCM RUO Purified 0.1 mg Cdc25C Hu C2-2 Ms IgG 1 Bioimg, WB RUO Purified 0.1 mg Cdc27 Dog, Hu, Ms, Rat 35 Ms IgG 2b IF, IP, WB RUO Purified 50 µg IF, IP, WB RUO Purified 150 µg Cdc34 Chick, Dog, Hu, Ms, Rat 17 Ms IgG 2a IF, WB RUO Purified 50 µg Cdc37 Dog, Hu, Ms, Rat 15 Ms IgG 1 IF, WB RUO Purified 50 µg Cdc42 Dog, Hu, Ms, Rat 44 Ms IgG 1 WB RUO Purified 50 µg WB RUO Purified 150 µg Cdk1 Hu, Ms, Rat 1 Ms IgG 1 IF, IHC(F)*, IP, WB RUO Purified 50 µg IF, IHC(F)*, IP, WB RUO Purified 150 µg Hu, Ms Polyclonal Rab Ig IP, WB RUO Serum 0.1 ml Cdk1/Cdc2 (py15) Hu, Ms, Rat 44 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Cdk2 Dog, Hu, Ms, Rat 55 Ms IgG 2a Bioimg, IF, IHC, IP, WB RUO Purified 50 µg Bioimg, IF, IHC, IP, WB RUO Purified 150 µg Hu Polyclonal Rab Ig WB RUO Serum 100 µl Hu, Ms Polyclonal Rab Ig WB RUO Serum 0.1 ml Cdk7 Hu MO-1 Ms IgG 2b, k IHC(Fr), WB RUO Purified 0.1 mg CHD3 Hu, Ms, Rat 44 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Chk2 Hu, Ms, Rat 19 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Cip1 (WAF1) Hu 70 Ms IgG 2a IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Cyclin A Hu 25 Ms IgG 1 Bioimg, IF, WB RUO Purified 50 µg Bioimg, IF, WB RUO Purified 150 µg BF683 Ms IgE FCM, IP, WB RUO Purified 100 µg FCM, IP, WB RUO Purified 250 µg FCM RUO FITC Set 100 tests FCM RUO PE Set 100 tests Cyclin A1 Hu B88-2 Ms IgG 1, k WB RUO Purified 100 µg Cyclin B Hu, Rat 18 Ms IgG 1 IF, IHC(F)*, WB RUO Purified 50 µg IF, IHC(F)*, WB RUO Purified 150 µg Cyclin B1 Hu GNS-11 Ms IgG 2a Bioimg, WB RUO Purified 100 µg Bioimg, WB RUO Purified 250 µg Polyclonal Rab IP, WB RUO Serum 100 µl Cyclin B1 (a.a 1-21) Hu, Ms GNS-1 Ms IgG 1 Bioimg, FCM, IF, IHC, IP, WB RUO Purified 100 µg Bioimg, FCM, IF, IHC, IP, WB RUO Purified 250 µg FCM RUO FITC Set 100 tests FCM RUO PE Set 100 tests Cyclin C Hu, Ms Polyclonal Rab Ig IP, WB RUO Serum 100 µg Cyclin D1 Hu DCS-6 Ms IgG 2a WB RUO Purified 100 µg G Ms IgG 1 FCM, IHC, IP, WB RUO Purified 100 µg FCM, IHC, IP, WB RUO Purified 250 µg FCM RUO FITC Set 100 tests Cyclin D2 Hu G Ms IgG 2a IP, WB RUO Purified 100 µg IP, WB RUO Purified 250 µg bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.

11 Apoptosis Reagents Cyclin D3 Dog, Hu, Ms, Rat 1 Ms IgG 2b IF, IHC, IP, WB RUO Purified 50 µg IF, IHC, IP, WB RUO Purified 150 µg Hu G Ms IgG 1 FCM, WB RUO Purified 100 µg FCM RUO FITC Set 100 tests Cyclins D1/D2/D3 Hu G Ms IgG 1 FCM, IP, WB RUO Purified 100 µg Cyclin E Hu HE12 Ms IgG 1 Bioimg, WB RUO Purified 50 µg Bioimg, WB RUO Purified 150 µg HE67 Ms IgG 2b IP RUO Purified 100 µg Cyclin F Hu B74-2 Ms IgG 1, k WB RUO Purified 100 µg Cytochrome c Hu, Ms, Rat 6H2.B4 Ms IgG 1, k Bioimg, IP RUO Purified 0.1 mg Hu, Ms, Rat, Hs, Pg 7H8.2C12 Ms IgG 2b, k WB RUO Purified 0.1 mg Hu 6H2.B4 Mouse IgG 1 Bioimg RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests D4-GDI Hu Polyclonal Rab Ig WB RUO Serum 0.1 ml DAP Kinase Hu 17 Ms IgG 1 IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg DAP3 Frog, Hu, Ms, Rat 10 Ms IgG 1 IF, IHC, WB RUO Purified 50 µg DFF40/CAD (a.a ) Hu, Ms, Rat Polyclonal Rab Ig WB RUO Purified 50 µg DFF45 Hu 19 Ms IgG 1 IF, WB RUO Purified 50 µg DNA Ligase III Dog, Hu, Ms, Rat 7 Ms IgG 1 WB RUO Purified 50 µg WB RUO Purified 150 µg DNA-PK (p350) Hu 4F10C5 Ms IgG 1, k IP, WB RUO Purified 0.1 mg DNA-PKcs (p350) Hu 6 Mouse IgG 2a IF, IHC, WB RUO Purified 50 µg DNA Polymerased Hu, Rat 22 Ms IgG 1 IF, WB RUO Purified 50 µg DNA Polymerase e Hu 34 Ms IgG 2b IF, WB RUO Purified 50 µg catalytic DNA Topoisomerase I Hu C-21.2 Ms IgM WB RUO Purified 0.1 mg E2F-1 Hu, Ms, Rat KH95/E2F Ms IgG 2a IP, WB RUO Purified 0.1 mg E2F-2 Hu TFE22 Ms IgG 1 WB RUO Purified 0.1 mg E2F-3 Hu TFE31 Ms IgG 1 WB RUO Purified 0.1 mg ERCC1 Hu 8F1 Ms IgG 2b WB RUO Purified 0.1 mg FADD Hu 1 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg A66-2 Ms IgG 1 IP, WB RUO Purified 0.1 mg FADD (ps194) Hu J Mouse (BALB/c) IgG 1, k WB RUO Purified 0.1 mg FBP Dog, Hu, Ms, Rat 6 Ms IgG 1 IF, WB RUO Purified 50 µg FEN-1 Dog, Hu, Ms, Rat 21 Ms IgG 1 Bioimg, IF, WB RUO Purified 50 µg Fractin Hu Polyclonal Rab Ig Bioimg, WB RUO Purified 100 µl GOK (Stim1) Hu, Ms, Rat 44 Ms IgG 2a IF, WB RUO Purified 50 µg Granzyme A Hu CB9 Ms IgG 1, k IC/FCM RUO Purified 0.1 mg IC/FCM RUO FITC Set 100 tests IC/FCM RUO PE Set 100 tests Granzyme B Hu, Rat 2CF/F5 Ms IgG 2a WB RUO Purified 50 µg Hu GB11 Ms IgG 1, k IC/FCM RUO Alexa Fluor mg IC/FCM RUO Alexa Fluor µg IC/FCM RUO Alexa Fluor mg IC/FCM RUO FITC 100 tests IC/FCM RUO BD Horizon V tests IC/FCM RUO PE 100 tests H2AX (ps139) Hu N1-431 Ms IgG 1, k Bioimg, WB RUO Purified 0.1 mg Bioimg, FCM RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor tests All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. 11 bdbiosciences.com

12 Apoptosis Product List Apoptosis Reagents Heme Oxygenase 1 Hu 23 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Heme Oxygenase 2 Hu 44 Ms IgG 2b IF, WB RUO Purified 50 µg hhr23b Dog, Hu 16 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg hilp (XIAP) Dog, Hu, Ms 28 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Frog, Hu, Ms, Rat 48 Ms IgG 1 IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg Histone H3 (ps28) Hu HTA28 Rat IgG 2a, k Bioimg RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests Hu, Ms HTA28 Rat IgG 2a, k IC/FCM RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests Ms HTA28 Rat IgG 2a, k IC/FCM RUO BD Horizon V tests HSF4 Hu, Ms 45 Ms IgG 1 IF, WB RUO Purified 50 µg Hsp40 Hu 5 Ms IgG 2a WB RUO Purified 50 µg Hsp60 Hu, Ms, Rat 24/HSP60 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg IF RUO FITC 50 µg Hu 24/HSP60 Ms IgG 1 Bioimg RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests Hsp70 Hu 5G10 Ms IgG 2b IP, WB RUO Purified 0.1 mg Chick, Dog, Hu, Ms, Rat 7 Ms IgG 1 IF, IP, WB RUO Purified 50 µg IF, IP, WB RUO Purified 150 µg Hsp70/Hsc70 Hu W27 Ms IgG 2a WB RUO Purified 50 µg Hsp75 (TRAP1) Dog, Hu, Ms, Rat 42 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Hsp90 Chick, Dog, Hu, Ms, Rat 68 Ms IgG 1 IF, IP, WB RUO Purified 50 µg Bov, Chick, Dog, Hu, Ms, Rat 68 Ms IgG 1 IF, IP, WB RUO Purified 150 µg Hsp110 Dog, Hu, Ms, Rat 21 Ms IgG 1 IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg I-FLICE Hu Polyclonal Rab IgG WB RUO Purified 50 µg ICAD Ms Polyclonal Rab IgG WB RUO Purified 0.2 ml c-iap-2 Hu F Ms IgG 1, k WB RUO Purified 50 µg WB RUO Purified 150 µg IkBa Hu 25 Mouse IgG 1 IF, IP, WB RUO Purified 50 µg IF, IP, WB RUO Purified 150 µg A920 Mouse IgG 1 IP, WB RUO Purified 50 µg IkBa (ps32/ps36) Hu 39A1413 Ms IgG 1 WB RUO Purified 50 µg IKKa Hu B78-1 Ms IgG 2b, k IP, WB RUO Purified 0.1 mg IKKb Dog, Hu 24 Ms IgG 1 IF, WB RUO Purified 50 µg Hu F Ms IgG 1, k IP, WB RUO Purified 50 µg IKKg Dog, Hu, Rat 54 Ms IgG 1 IF, WB RUO Purified 50 µg Hu, Ms C Ms IgG 1, k IP, WB RUO Purified 0.1 mg Hu, Ms, Rat C Ms IgG 1 WB RUO Purified 0.1 mg Itch Hu, Ms, Rab, Rat 32 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg JNK (pt183/py185) Hu 41 Ms IgG 1 WB RUO Purified 50 µg WB RUO Purified 150 µg Polyclonal Rabbit IgG IC/FCM, IHC(F)*, WB RUO Purified 50 tests Hu, Ms N9-66 Ms IgG 1, k κ IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests JNK/SAPK1 Chick, Dog, Frog, Hu, Ms, Rat 37 Ms IgG 1 IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.

13 Apoptosis Reagents JNK1 Hu G Ms IgG 1 WB RUO Purified 50 µg WB RUO Purified 150 µg JNK1/JNK2 Hu G Ms IgG 2a IP, WB RUO Purified 0.1 mg JNKK1 Hu G Ms IgG 1 IP, WB RUO Purified 0.1 mg Ki-67 Hu 35 Ms IgG 1 Bioimg, IF, WB RUO Purified 50 µg Bioimg, IF, WB RUO Purified 150 µg IF RUO FITC 50 µg Hu, Ms, Pig, Rat B56 Ms IgG 1, k IC/FCM, Bioimg RUO Purified 0.1 mg IHC(F)*, IHC(Fr) RUO Purified 1 ml IC/FCM RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor tests Bioimg RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor tests IC/FCM RUO FITC Set 100 tests IC/FCM RUO BD Horizon V tests IC/FCM RUO PE Set 100 tests IC/FCM RUO PerCP-Cy tests Ku70 Hu, Ms, Rat 15 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Ku80 Hu 7 Ms IgG 1 IF, WB RUO Purified 50 µg LITAF Hu 30 Ms IgG 1 IF, WB RUO Purified 50 µg LT-a Hu Ms IgG 1 ELISA Cap. RUO Purified 1.0 mg Ms IgG 1 ELISA Det. RUO Biotin 0.5 mg IC/FCM RUO PE 0.1 mg Mcl-1 Hu 22 Ms IgG 1 k IP, WB RUO Purified 0.1 mg Polyclonal Rab Ig WB RUO Serum 0.1 ml pan-mcm Hu Polyclonal Rab Ig WB RUO Serum 0.1 ml MCM4 Hu, Ms Polyclonal Rab Ig WB RUO Serum 0.1 ml MCM6 Hu Ki-Mcm6 Ms IgG 1, k FCM RUO FITC 100 tests Ms IgG 1, k Bioimg, IF, WB RUO Purified 50 µg MDM2 Hu SMP14 Ms IgG 1 Bioimg, IF, IP, WB RUO Purified 0.1 mg MEK1 Hu 25/MEK1 Ms IgG 2a Bioimg, IF, IHC, IP, WB RUO Purified 50 µg Bioimg, IF, IHC, IP, WB RUO Purified 150 µg IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests MEK1 (ps298) Hu, Ms J Mouse (BALB/c) IgG 1, k WB RUO Purified 0.1 mg IC/FCM RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests MEK1 (ps218) Hu, Ms O Ms IgG 1, k IC/FCM, WB RUO Purified 0.1 mg /MEK2 (ps222) IC/FCM RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests MEK1/2 (ps222) Hu Polyclonal Rabbit IgG IC/FCM, IHC(F)*, WB RUO Purified 50 tests MEK2 Chick, Dog, Frog, Hu, Ms, Rat 96/MEK2 Ms IgG 2a IF, IP, WB RUO Purified 50 µg IF, IP, WB RUO Purified 150 µg Hu 96/MEK2 Ms IgG 2a IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 0.1 mg Hu, Ms A7-1 Ms IgM WB RUO Purified 0.1 mg MGMT Hu MT5.1 Ms IgG 1 k FCM, IP, WB RUO Purified 0.1 mg All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. 13 bdbiosciences.com

14 Apoptosis Product List Apoptosis Reagents MLH1 Hu, Ms G Ms IgG 2a k IP, WB RUO Purified 0.1 mg IHC(Fr), IHC(P), WB RUO Purified 50 µg IHC(Fr), IHC(P), WB RUO Purified 150 µg G Ms IgG 1 IHC(Fr), IHC(P), WB RUO Purified 1 ml Mre11 Hu, Ms, Rat 18 Ms IgG 1 IF, WB RUO Purified 50 µg MSH2 Hu G Ms IgG 1 IHC(F), IHC(Fr), WB RUO Purified 0.1 mg MSH3 Hu 52 Ms IgG 1 IF, WB RUO Purified 50 µg MSH6 Dog, Hu, Ms, Rat 44 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg c-myc Hu 9E10 Ms IgG 1 WB RUO Purified 50 µg WB RUO Purified 150 µg NAT1 (DAP-5) Bov, Dog, Hu, Ms, Rat 35 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg NBS1 Chick, Dog, Hu, Ms, Rat 34 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Nedd4 Ms 15 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Nip1 Hu, Rat 5 Ms IgG 1 IF, WB RUO Purified 50 µg Nucleosome Ms, Hu 11E6 Ms IgG 2b k IF RUO Purified 50 µg p21 Hu 2G12 Ms IgG 1 k IHC(P), IP, WB RUO Purified 0.1 mg B6 Ms IgG 1 IHC(Fr), IHC(P), IP, WB RUO Purified 0.1 mg Hu, Ms, Rat SX118 Ms IgG 1 k IF, IHC(Fr), IHC(P), IP, WB RUO Purified 0.1 mg SXM30 Ms IgG 1 k IF, IHC(Fr), IHC(P), IP, WB RUO Purified 0.1 mg p33 ING1 Ms, Hu CAb1 Ms IgG 2a WB RUO Purified 0.1 mg p53 Dog, Hu 80 Ms IgG 2b, k IF, IP, WB RUO Purified 50 µg IF, IP, WB RUO Purified 150 µg Bov, Hu DO-1 Ms IgG 2a IHC(F), IHC(Fr), IP, WB RUO Purified 0.1 mg Bov, Hu, Monk DO-7 Ms IgG 2b FCM, IHC(F), IHC(Fr), IP, WB RUO Purified 0.1 mg FCM RUO FITC Set 100 tests FCM RUO PE Set 100 tests Hu, Ms, Rat G59-12 Ms IgG 1 IHC(F), IHC(Fr), IP, WB RUO Purified 0.1 mg FCM RUO FITC Set 100 tests FCM RUO PE Set 100 tests Ham, Hu, Monk, Ms, Rat PAb 122 Ms IgG 2b FCM, IF, IP, WB RUO Purified 0.1 mg Bov, Chick, Ham, Hu, PAb 240 Ms IgG 1 IHC(Fr), IP, WB RUO Purified 0.1 mg Monk, Ms, Rat IHC(Fr), IP, WB RUO Purified 0.25 mg Hu PAb 1801 Ms IgG 1 IF, IHC(Fr), IHC(P), IP, WB RUO Purified 0.1 mg IF, IHC(Fr), IHC(P), IP, WB RUO Purified 0.25 mg p53 (ack382) Hu L82-51 Mouse (BALB/c) IgG 1, k IC/FCM, WB RUO Alexa Fluor tests p53 (ps37) Hu J Mouse IgG1, k IHC(F)*, WB RUO Purified 0.1 mg p53 (ps46) Hu I Mouse (BALB/c) IgG 1, k IHC(F)*, WB RUO Purified 0.1 mg Poly ADP-Ribose (PAR) Hu 10H Mouse IgG 3 IF RUO Purified 50 µg PAR [Poly(ADP-Ribose)] Ms, Hu Polyclonal Rab Ig WB RUO Purified 0.1 ml PARP Hu C2-10 Ms IgG 1 WB RUO Ascites 0.1 ml C10-5 Ms IgG 1 Bioimg, WB RUO Purified 0.1 mg D3-6 Ms IgG 1 WB RUO Purified 50 µg WB RUO Purified 150 µg Dog, Hu, Ms, Rat 42 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.

15 Apoptosis Reagents PARP, Cleaved Form Hu, Ms F Ms IgG 1, k IC/FCM, IP, WB RUO Purified 50 µg (Asp 214) IC/FCM, WB RUO Purified 150 µg Bioimg RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor tests IC/FCM RUO FITC 100 tests IC/FCM RUO PE 100 tests PCNA Dog, Hu, Ms, Rat 24 Ms IgG 1 IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg Hu PC10 Ms IgG 2a, k IHC(F), IC/FCM RUO Purified 0.1 mg IC/FCM RUO Biotin 0.1 mg IC/FCM RUO FITC Set 100 tests IC/FCM RUO PE Set 100 tests PDPK1 Dog, Hu, Ms, Rat 5 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg PDPK1 (ps241) Hu J Mouse IgG 1, k IC/FCM RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests J Mouse IgG 1, k WB RUO Purified 0.1 mg Perforin Hu dg9 Ms IgG 2b FCM, IF, IHC(Fr), IP RUO Purified 0.1 mg FCM RUO FITC Set 100 tests FCM RUO PE Set 100 tests Peroxiredoxin V Rat 44 Ms IgG 1 WB RUO Purified 50 µg PKC Bov, Chick, Hu, Ms, Rab, Rat MC5 Ms IgG 2a WB RUO Purified 0.1 mg PKR (p68 Kinase) Hu 13 Ms IgG 1 IF, WB RUO Purified 50 µg PLK1 (pt210) Hu K Ms IgG 1, k WB RUO Purified 0.1 mg IC/FCM RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests PMS2 Hu, Ms A16-4 Ms IgG 1 IF, IP, WB RUO Purified 0.1 mg PTEN Hu A2B1 Ms IgG 1, k WB RUO Purified 100 µg IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests Ras Chick, Dog, Hu, Ms, Rat 18 Mouse IgG 1 IF, IHC, IP, WB RUO Purified 150 µg IF RUO FITC 50 µg IF RUO FITC 150 µg R-ras Ms Polyclonal Rab IgG IF, IHC(Fr), IHC(P), WB RUO Serum 0.1 ml Ras-GAP Chick, Dog, Frog, Hu, Ms, Rat 13 Mouse IgG 1 IF, IHC, WB RUO Purified 50 µg Ras-GAP Chick, Dog, Frog, Hu, Ms, Rat 13 Mouse IgG 1 IF, IHC, WB RUO Purified 150 µg Rad50 Hu 13 Ms IgG 1 IF, WB RUO Purified 50 µg hrad9 Hu, Ms, Rat 56 Ms IgG 1 IF, WB RUO Purified 50 µg Rb (a.a ) Hu G Ms IgG 1 IP, WB RUO Purified 0.1 mg Rb (a.a ) Hu G3-349 Ms IgG 1 WB RUO Purified 0.1 mg Rb (a.a ) Hu G3-245 Ms IgG 1 Bioimg, WB RUO Purified 0.1 mg IC/FCM RUO FITC Set 100 tests Rb (a.a ) Hu XZ55 Ms IgG 1 WB RUO Purified 0.1 mg Rb (a.a ) Hu XZ91 Ms IgG 2a WB RUO Purified 0.1 mg Rb (ps780) Hu J Ms IgG 1, k IHC(F)*, WB RUO Purified 0.1 mg Rb, Underphosphorylated Hu G Ms IgG 1 IP, WB RUO Purified 0.1 mg (a.a ) IC/FCM RUO FITC Set 100 tests IC/FCM RUO PE Set 100 tests Rb (ps780) Hu J Mouse (BALB/c) IgG 1, k IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. 15 bdbiosciences.com

16 Apoptosis Product List Apoptosis Reagents Rb (ps807/ps811) Hu J Mouse (BALB/c) IgG 2a, k WB RUO Purified 0.1 mg IC/FCM RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor tests IC/FCM RUO PE 50 tests Rb2 Chick, Hu, Ms, Rat, Dros 10 Ms IgG 2a IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg RCC1 Dog, Hu 9 Ms IgG 1 IF, IHC, WB RUO Purified 50 µg Ref-1 Dog, Hu, Ms, Rat 15 Ms IgG 1 IF, WB RUO Purified 50 µg RIP Chick, Dog, Hu, Ms, Rat 38 Ms IgG 2a IF, IP, WB RUO Purified 50 µg IF, IP, WB RUO Purified 150 µg Hu G322-2 Ms IgG 1, k WB RUO Purified 50 µg WB RUO Purified 150 µg RIP2/RICK Hu 25 Ms IgG 1 WB RUO Purified 50 µg WB RUO Purified 150 µg Smac/DIABLO Dog, Hu, Ms, Rat 7 Ms IgG 1 Bioimg, IF, WB RUO Purified 50 µg Bioimg, IF, WB RUO Purified 150 µg Ms IgG 2b WB RUO Purified 50 µg Smad1 (ps463/ps465)/ Hu N6-1233, 1233 Rat IgG 2a, k IC/FCM, WB RUO Purified 0.1 mg Smad8 (ps465/ps467) IC/FCM RUO Purified 50 tests Smad2 (ps465/ps467)/ Hu O Ms IgG 1, k IC/FCM RUO Purified 50 tests Smad3 (ps423/ps425) Smad2/3 Dog, Hu, Ms, Rat 18 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg SREBP-1 Hu, Ham IgG-2A4 Ms IgG 1, k IP, WB RUO Purified 0.1 mg SREBP-2 Hu IgG-1C6 Ms IgG 1, k IP, WB RUO Purified 0.1 mg STI1 (Hop-p60) Dog, Hu 28 Ms IgG 1 IF, WB RUO Purified 50 µg SOD (Superoxide Hu G215-1 Ms IgG 2a WB RUO Purified 0.1 mg Dismutase) TIAR Dog, Frog, Hu, Ms, Rat 6 Ms IgG 1 IF, IHC, IP, WB RUO Purified 50 µg Tim23 Hu, Ms, Rat 32 Ms IgG 2a IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg TNF-a Hu Ms IgG 1, k FCM, IC RUO(GMP) APC 100 tests FCM, IC RUO(GMP) FITC 50 tests FCM, IC RUO(GMP) PE 50 tests TNF Hu Mab1 Ms IgG 1 Neu RUO NA/LE 0.5 mg ELISA Cap. RUO Purified 1.0 mg Bab, Cyno, Rhe MAb11 Ms IgG 1, k IC/FCM RUO Purified 0.1 mg IC/FCM RUO APC 50 tests IC/FCM RUO FITC 50 tests IC/FCM RUO PE 50 tests Hu MAb11 Ms IgG 1, k IC/FCM Block RUO Purified 0.1 mg ICC RUO Purified 0.25 mg IC/FCM RUO Alexa Fluor tests IC/FCM RUO Alexa Fluor µg IC/FCM RUO APC 0.1 mg ELISA Det. RUO Biotin 0.5 mg IC/FCM RUO FITC 0.1 mg IC/FCM RUO PE 0.1 mg IC/FCM RUO PE 100 tests IC/FCM RUO PE-Cy7 25 tests FCM* RUO PE-Cy7 100 tests IC/FCM RUO PerCP-Cy tests MABTNF-A5 Ms IgG 2a, k Neu RUO NA/LE 0.5 mg Ms, Rat G Rat IgG 1 Neu RUO NA/LE 0.5 mg ELISA Cap., WB RUO Purified 1.0 mg bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.

17 Apoptosis Reagents TNF (continued) Ms MP6-XT3 Rat IgG 1 Neu RUO NA/LE 0.5 mg ELISA Det., WB RUO Biotin 0.5 mg MP6-XT22 Rat IgG 1 ELISA Cap., IC/FCM Block RUO Purified 0.1 mg ICC RUO Purified 0.25 mg IC/FCM RUO Alexa Fluor mg IC/FCM RUO Alexa Fluor mg IC/FCM RUO Alexa Fluor mg IC/FCM RUO APC 25 µg IC/FCM RUO APC 0.1 mg IC/FCM RUO APC-Cy7 50 µg IC/FCM RUO FITC 25 µg IC/FCM RUO FITC 0.1 mg IC/FCM RUO BD Horizon V µg IC/FCM RUO PE 25 µg IC/FCM RUO PE 0.1 mg IC/FCM RUO PE-Cy7 25 µg IC/FCM RUO PE-Cy7 0.1 mg IC/FCM RUO PerCP-Cy µg D1A1 Human Fab Ig ELISA Det. RUO Biotin 0.5 mg Polyclonal Rab Ig ELISA Det. RUO Biotin 0.5 mg Ms, Rat TN Ar Ham IgG1, l1 IP, Neu, WB RUO NA/LE 0.5 mg ELISA Cap., IC/FCM Block, RUO Purified 0.5 mg IP, WB TN Ar Ham IgG1, l1 IC/FCM RUO PE 0.1 mg Rab 23H1.1 Ms IgG 1, k ELISA Det. RUO Biotin 0.5 mg Polyclonal Gt IgG ELISA Cap. RUO Purified 1.0 mg TopBP1 Hu 33 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg Topo IIa Hu 31 Ms IgG 1 Bioimg, IF, WB RUO Purified 50 µg Bioimg, IF, WB RUO Purified 150 µg Topo IIb Hu 40 Ms IgG 1 Bioimg, IF, WB RUO Purified 50 µg Bioimg, IF, WB RUO Purified 150 µg TRADD Hu, Rat 37 Ms IgG 1 IF, IHC, IP, WB RUO Purified 50 µg IF, IHC, IP, WB RUO Purified 150 µg Hu B36-2 Ms IgG 1 WB RUO Purified 0.1 mg TRAF3 Hu B1-6 Ms IgG 1, k WB RUO Purified 0.1 mg TRF2 Dog, Hu 36 Ms IgG 1 WB RUO Purified 50 µg Ubc9 Chick, Dog, Hu, Ms, Rat 50 Ms IgG 2a IF, IHC, WB RUO Purified 50 µg IF, IHC, WB RUO Purified 150 µg UbcH6 Dog, Hu, Ms, Rat 42 Ms IgG 1 IF, WB RUO Purified 50 µg UbcH7 Dog, Frog, Hu, Ms, Rat 20 Ms IgG 1 IF, WB RUO Purified 50 µg IF, WB RUO Purified 150 µg XPA Hu 12F5 Ms IgG 2a, k IP, WB RUO Purified 0.1 mg XRCC4 Hu 4 Ms IgG 2b IF, WB RUO Purified 50 µg All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. 17 bdbiosciences.com

18 Apoptosis Product List Proteins description react apps reg format size cat. no. Annexin V Block, FCM RUO Purified 0.1 mg FCM RUO Biotin 100 tests FCM RUO Biotin 200 tests FCM RUO APC 25 tests FCM RUO APC 100 tests FCM RUO APC 200 tests FCM RUO Cy5 100 tests FCM RUO Cy5 200 tests FCM RUO Cy tests FCM RUO FITC 25 tests FCM RUO FITC 100 tests FCM RUO FITC 200 tests FCM RUO BD Horizon V tests FCM RUO BD Horizon V tests FCM RUO PE 25 tests FCM RUO PE 100 tests FCM RUO PE 200 tests Caspase-3, Active Form, FA, SF RUO Purified 5 µg Recombinant Human Protein FA, SF RUO Purified 10 µg Caspase-8, Active Form, FA, SF RUO Purified 5 µg Recombinant Human Protein FA, SF RUO Purified 10 µg Fas (Soluble) Hu WB RUO Purified 10 µg Recombinant Human Protein (FasdTM) Fas:Fc Chimeric IP RUO Purified 0.1 mg Fusion Protein LT-a, Recombinant Hu ELISA Std., FA RUO Purified 10 µg human p53 (Wildtype) WB RUO Purified 10 µg Recombinant Human Protein TNF, Recombinant human Hu ELISA Std., FA RUO Purified 10 µg TNF, Recombinant mouse Ms ELISA Std., FA, IC/FCM Block RUO Purified 10 µg TNF, Recombinant Rabbit Rab ELISA RUO Purified 10 tests TNF, Recombinant rat Rat ELISA Std., FA RUO Purified 5 µg Apoptosis Product List Peptides description apps reg format size cat. no. Caspase Inhibitor FA RUO Lyophilized 1.0 mg Caspase Inhibitors Negative Control, Z-FA-FMK FA, SF RUO Purified 1.0 mg Caspase-3 Fluorogenic Substrate, Ac-DEVD-AFC SF, FA RUO Purified 1.0 mg Caspase-3 Fluorogenic Substrate, Ac-DEVD-AMC SF, FA RUO Purified 1.0 mg Caspase-3 Inhibitor, Ac-DEVD-CHO SF, FA RUO Purified 1.0 mg Caspase-3 Inhibitor, Z-DEVD-FMK FA, SF RUO Purified 1.0 mg Caspase-8 Fluorogenic Substrate, Ac-IETD-AFC SF, FA RUO Purified 1.0 mg Caspase-8 Inhibitor, Ac-IETD-CHO SF, FA RUO Purified 1.0 mg Caspase-8 Inhibitor, Z-IETD-FMK FA, SF RUO Purified 1.0 mg Caspase-9 Inhibitor, Z-LEHD-FMK FA, SF RUO Purified 1.0 mg General Caspase Inhibitor, Z-VAD-FMK FA, SF RUO Purified 1.0 mg bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details.

19 Buffers/Solutions description apps reg format size cat. no. 7-AAD Staining Solution FCM RUO Solution 2 ml Annexin V Binding Buffer, 10X Concentrate FM, FCM RUO Buffer 50 ml BrdU Solution RUO Solution 25.0 mg Cell Lysis Buffer RUO Buffer 50 ml Cell Viability Solution FCM RUO Solution 100 tests FCM RUO Solution 500 tests BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit IC/FCM RUO Fixation/Permeabilization 250 tests solution (125 ml) and BD Perm/Wash buffer, 10X concentrate containing Fetal Bovine Serum (FBS) and saponin (100 ml) BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit with BD GolgiPlug IC/FCM RUO Fixation/Permeabilization 250 tests solution (125 ml), BD Perm/Wash buffer, 10X concentrate containing Fetal Bovine Serum (FBS) and saponin (100 ml), and BD GolgiPlug protein transport inhibitor (containing brefeldin A) BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit with BD GolgiStop IC/FCM RUO Fixation/Permeabilization 250 tests solution (125 ml), BD Perm/Wash buffer, 10X concentrate containing Fetal Bovine Serum (FBS) and saponin (100 ml), and BD GolgiStop protein transport inhibitor (containing monensin) Fixation/Permeabilization Solution IC/FCM RUO Solution 250 tests PI/RNase Staining Buffer FCM RUO Buffer 100 ml Propidium Iodide Staining Solution FCM RUO Solution 2 ml Protein Transport Inhibitor (containing brefeldin A) IC/FCM RUO Solution 250 tests All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. 19 bdbiosciences.com

20 For Research Use Only. Not for use in diagnostic or therapeutic procedures. CF is a trademark of Biotium, Inc. Cy is a trademark of Amersham Biosciences Corp. Cy dyes are subject to proprietary rights of Amersham Biosciences Corp and Carnegie Mellon University and are made and sold under license from Amersham Biosciences Corp only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from Amersham Biosciences Corp, 800 Centennial Avenue, Piscataway, NJ , USA. Alexa Fluor is a registered trademark of Molecular Probes, Inc. BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company BD BD Biosciences bdbiosciences.com