Product and Pricelist
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1 Product and Pricelist valid to December 2008
2 Introduction This catalogue contains test kits and components for the rapid detection of biologial agents such as toxins, micro organisms and viruses. The testkits are based on the ABICAP technology (ABICAP = Antibody Binding Immune Column for Analytical Purpose). ABICAP is a flow through immuno assay system for rapid and ultra sensitive detection. It is based on the 3D-immunofiltration 1 and PolyHRP-technology 2 and allows to combination of analyte extraction and direct quantitative read out on the test columns. The system is well suited for decentralised routine testing. To this end ready to use test kits, equipment (workstation in 96 hole format) and a handheld photometer are offered. In addition, for research studies and customer specific development, assay components and reagents are available and development services are offered. 1 see booklet: "introduction to 3D Immunofiltration technology" 2 see booklet: "introduction to polyhrp technology"
3 Index Index ABICAP classic Test Kits Microorganisms... Viruses... Toxins... ABICAP multilayer Test Kits Microorganisms... Viruses... Toxins... Auxiliary reagents and components for ABICAP Test Kits Positive controls lyophilized pellets... Special buffers and blocker... Sample preparation tools... Equipment ABICAP Photometer... Workstations... Antibodies Microorganisms... Viruses... Toxins... Antigens Microbiological agents... Toxins... Assay components and tools Microcolumns Buffer PolyHRP Substrate PolyHRP Reagents Specific HRP- and PolyHRP Conjugates Services ABICAP custom development PolyHRP custom conjugation From a order value of 5000,00 or higher, shipping costs are included. Terms and conditions could be found on our website
4 ABICAP Test Kits ABICAP classic Test Kits The ABICAP classic Test kit contains 24 ABICAP columns with one specific detection filter and all reagents and instructions needed to perform quantitative assays. negative weak positive strong positive 2 Microorganisms CAP Francisella tularensis ABICAP Classic 480, CAP Yersinia pestis F1 ABICAP Classic 480, CAP B. anthracis spores ABICAP Classic 480, CAP B. anthracis protective antigen ABICAP Classic 480,00 Viruses CAP Ebola virus ABICAP Classic 480, CAP Flaviviruses ABICAP Classic 480, CAP Venezuela Equine Encephalitis (VEE) ABICAP Classic (under development) CAP Eastern Equine Encephalitis (EEE) ABICAP Classic (under development) Toxins CAP Botulinus toxin A ABICAP Classic CAP Botulinus toxin B ABICAP Classic 480, CAP Botulinus toxin E ABICAP Classic 480, CAP Staphylococcus Enterotoxin B (SEB) ABICAP Classic 480, CAP Ricin ABICAP Classic 480,00
5 ABICAP multilayer Test Kits The ABICAP multilayer test kit contains 24 ABICAP columns in a 3 filter arrangement and all reagents to perform quantitative tests. negative weak positive strong positive Negative control Filter (indicates non specific binding) 3 Specific Measurement filter Positive control Filter (indicates function of assay reagents Microorganisms CAP Francisella tularensis ABICAP multilayer 720, CAP Yersinia pestis ABICAP multilayer 720,00 Toxins CAP Botulinus toxin A ABICAP multilayer 720, CAP Botulinus toxin B ABICAP multilayer 720, CAP Botulinus toxin E ABICAP multilayer 720, CAP Staphylococcus Enterotoxin B (SEB) ABICAP multilayer 720, CAP Ricin ABICAP multilayer 720,00
6 Auxiliary reagents and components for ABICAP Test Kits Auxiliary reagents and components for ABICAP Test Kits Positive contols Positive controls are lyophilised reagent pellets. They are reconstituted in destilled water. Microorganisms PC Legionella pneumophila serotyp 1 2 controls (under development) PC Francisella tularensis 2 controls 12, PC F1 control (Yersinia pestis) 2 controls 12, PC B. anthracis spores 2 controls 14, PC B. anthracis protective antigen 2 controls 14,00 4 Viruses PC Ebola control 2 controls 12, PC Flaviviruses 2 controls (under development) PC Venezuela Equine Encephalitis (VEE) 2 controls (under development) PC Eastern Equine Encephalitis (EEE) 2 controls (under development) Toxins PC Botulinus- toxin A (toxoid) 2 controls 12, PC Botulinus- toxoid B (toxoid) 2 controls 12, PC Botulinus- toxoid E (toxoid) 2 controls 12, PC Staphylococcus Enterotoxin B (SEB) 2 controls 12, PC Ricin 2 controls 14,00
7 Auxiliary reagents and components for ABICAP Test Kits Special buffers and blocker BUF HAMA - Blocker* (Human anti-mouse antibody) BUF LPS Extraction buffer** (Lipopolysaccharid) 10 mg mg 100 mg 50 ml 100 ml 25,00 20,00/10mg 175,00 15,00 25,00 *Human anti-mouse antibodies might course false positive signals. HAMA-Blocker reduces the frequency of false results by blocking a wide range of HAM antibodies regardless of the anti-mouse epitope **Lipopolysaccharid is a useful buffer to extract LPS from gram-negative cells; thougt for sample preparations in the special case of the Francisella tularensis assay Sample preparation tools 5 For the treatment of complex sample material and prefiltration we offer sample preparation tools. They have to be selected by the customers in dependence on the sample matrix. For large volume application sample funnels (figure 3,4) are offered. Productnumber Product Quantity Price in CMP sorbent filter (fig. 1) 5 20, CMP 10-20µm PE ABICAP prefilter 24 24, CMP 20-60µm PE ABICAP prefilter 24 24, CMP µm PE ABICAP prefilter 24 24, CMP µm PE ABICAP prefilter 24 24, CMP filter holder for prefilter (fig. 2) 10 60, CMP sample application funnel, 10 ml (fig. 3) 6 15, CMP sample application funnel, 50 ml (fig. 4) 12 39,
8 Equipment Equipment ABICAP Photometer (not included in the Test Kits) Productnumber Product Quantity Price in RDR ABICAP Photometer (portable) for ABICAP Classic and ABICAP Multilayer with key pad, incl. chip card reader, power supply, smart card reader, RS232 interface, weight approx. 300g, dimension 18x 9x3cm (fig. 5) 1 840,00 Accessories: RDR PC software and cable 1 179,00 Workstations (not included in the Test Kits) 6 Productnumber Product Quantity Price in CMP ABICAP Rack for 96 ABICAP Microcolumns consisting of one 96-hole frame, a stackable frame and one waste basket (fig. 6) 1 39, CMP Stackable frame for ABICAP Rack, plexiglas 1 20, CMP Stackable frame for ABICAP Rack, paperboard, disposable (fig. 7) CMP Waste basket for ABICAP Rack, sterilizable (fig. 8) 25 5, , CMP 96-hole frame to hold ABICAP Microcolumns 1 4,
9 Antibodies Antibodies For all antibodies listed application notes for ELISA are available. Microorganisms Productnumber Product Amount Price in /mg MAB Monoclonal anti-bacillus anthracis Protective Antigen clon 249 (for coating) IgG MAB Monoclonal anti-bacillus anthracis Protective Antigen clon 352 (for conjugation) IgG MAB Monoclonal anti-bacillus anthracis Protective Antigen, biotinylated clon 352 IgG1 480, MAB Monoclonal anti-bacillus anthracis spores, clon 38 (for coating) IgG2a 100 to 249 mg > 250 mg 280,00 210, MAB Monoclonal anti-bacillus anthracis spores, clon 37 (for conjugation) IgG2a 100 to 249 mg > 250 mg 280,00 210, MAB Monoclonal anti-bacillus anthracis spores, clon 37, biotinylated IgG2a 620, MAB Monoclonal anti-francisella tularensis, Reacts with LPS of F. tularensis IgG MAB Monoclonal anti-francisella tularensis, biotinylated Reacts with LPS of F. tularensis IgG3 420, PAB Polyclonal anti-legionella pneumophilia, Serotyp 1, G , MAB Monoclonal anti-legionella pneumophilia, Serotyp 1, 6G MAB Monoclonal anti-legionella pneumophila, Serotyp 1 Reacts with LPS membrane of Legionella pneumophila, IgG MAB Monoclonal anti-staphylococcus aureus Enterotoxin B, Specific for enterotoxins, B, no crossreactivity with C, D, E, clon 333 (for coating), IgG1
10 Antibodies MAB Monoclonal anti-staphylococcus aureus Enterotoxin B, Specific for enterotoxins, B, no crossreactivity with C, D, E, clon 754 (for conjugation), IgG MAB Monoclonal anti-staphylococcus aureus Enterotoxin B, biotinylated Specific for enterotoxins, B, no crossreactivity with C, D, E, clon 754 (for conjugation), IgG MAB Monoclonal anti-yersinia pestis-f1 Antigen, Reacts with capsule bearing strains of Y. pestis, IgG MAB Monoclonal anti-yersinia pestis-f1 Antigen, biotinylated Reacts with capsule bearing strains of Y. pestis, IgG1 Viruses 420,00 420,00 Productnumber Product Amount Price in /mg MAB Monoclonal anti-ebola Virus, (for coating) IgG MAB Monoclonal anti-ebola Virus, (for conjugation) IgG MAB Monoclonal anti-ebola Virus, IgG, biotinylated PAB Polyclonal anti-venezuela Equine Encephalitis affinity purified mouse IgG PAB Polyclonal anti-eastern Equine Encephalitis affinity purified mouse IgG PAB Polyclonal anti-western Equine Encephalitis affinity purified mouse IgG MAB Monoclonal anti-flavivirus, group specific IgG2a MAB Monoclonal anti-flavivirus, group specific IgG2a, biotinylated 280,00 210,00 280,00 210,00 620,00 280,00 210,00
11 Antibodies Toxins Productnumber Product Amount Price in /mg MAB Monoclonal anti-botulinus A clon 22G8, (for coating) IgG2a/IgM MAB Monoclonal anti-botulinus B clon C3/4 (for coating) IgG MAB Monoclonal anti-botulinus E clon 21D4 (for coating) IgG PAB Polyclonal anti-botulinus A horse, F(ab) 2 fragments PAB Polyclonal anti-botulinus A, biotinylated horse, F(ab) 2 fragments PAB Polyclonal anti-botulinus B horse, F(ab) 2 fragments PAB Polyclonal anti-botulinus B, biotinylated horse, F(ab) 2 fragments PAB Polyclonal Botulinus E horse, F(ab) 2 fragments PAB Polyclonal Botulinus E, biotinylated horse, F(ab) 2 fragments 100µg 100µg 100µg PAB Polyconal anti-botulinus E rabbit IgG fraction 100µg 100,00 520,00 440,00 100,00 520,00 440,00 100,00 520,00 440,00 160,00 90,00 310,00 160,00 90,00 310,00 160,00 90,00 288,00 50,00 9
12 Antigens Antigens Microbiological Antigens Productnumber Product Amount Price in AGN Bacillus anthracis Protective Antigen (PA), isolated from the vaccine strain STI AGN Bacillus anthracis spores attenuated, strain, inactivated 100 µg 10 6 spores 10 9 spores AGN Y.pestis F1 Antigen 100 µg AGN F. Tularensis, LPS Antigen 100 µg 85,00 480,00 150,00 780,00 85,00 480,00 85,00 480, AGN Legionella pneumophila serogroup 1 LPS 10 Toxins Productnumber Product Amount Price in AGN Staphylococcus aureus Enterotoxin B, purified 100 µg AGN Ricin, Ammoniumsulphate precipitated 100 µg 65,00 85,00 480,00
13 Assay components and tools Assay components and tools Many reagents used in conventional immunoassay can be applied to the 3D-Immunofiltration formate. The coating procedure of the porous filters is simple and allows the manufacturing of your own individual column assays. Microcolumns Productnumber Product Quantity Price in CMP Microcolumns with frits for protein coating in packages of 192 columns 1-10 packs packs >1000 packs 0,62/column 0,52/column Buffer 11 Productnumber Product Amount Price in CAS Casein Diluent / Blocker, ready-to-use (UCDB) 500 ml ml ml ml ml ml ml ml ml BUF Blocking buffer 500 ml 1000 ml 2000 ml > 2000 ml BUF PolyHRP Conjugate Stabilizer, ready-to-use 500 ml ml ml ml ml ml ml ml ml BUF Substrate buffer 500 ml ml ml ml ml ml ml ml ml 150,00 270,00 500,00 800, , , , , ,00 150,00 270,00 500,00 330,00 600, , , , , , ,00 20,00 30,00 50,00 90,00 100,00 150,00 250,00 300,00 380,00
14 Assay components and tools PolyHRP Substrate Productnumber Product Amount Price in SUB stmb substrate solution, soluble, for ELISA-applications; ready-to-use SUB ptmb substrate solution, precipitating, for 3D-IF rapid test applications (ABICAP ); ready-to-use PolyHRP Reagents 100 ml 250 ml 500 ml 1000 ml 50 ml 100 ml 250 ml 500 ml 1000 ml 30,00 80,00 135,00 235,00/L 90,00 110,00 190,00 290,00 450,00 Productnumber Product Amount Price in /mg CJG SA-PolyHRP 40 2 to 9 mg 100 to 599 mg > ,00 310,00 280,00 260,00 12 PolyHRP is a supermolecular enzyme complex with up to 400 molecules. The PolyHRP complex can be conjugated to various ligands and receptors. These PolyHRP-conjugates can be utilized in ELISA assays, immunoblots, immunofiltration, DNA/RNA Hybridisation. Its specific activity is by magnitudes superior compared to conventional conjugates, allowing for an substantial inhancement of assay sensitivity, optimization of assay time and reduction of reagent consumption. Specific HRP- and PolyHRP Conjugates Productnumber Product Amount Price in CJG Monoclonal anti-y. Pestis-F1 - PolyHRP µg 0,5 mg > CJG Monoclonal anti-f. Tularensis - PolyHRP µg 0,5 mg > CJG Monoclonal anti-ebola Virus - PolyHRP µg 0,5 mg CJG Monoclonal anti-b.anthracis spores HRP 100 µg 0,5 mg > 300, , ,00 300, , ,00 380, , ,00 150,00 600,00 900,00
15 Services Services 3D-Immunofiltration assays and the PolyHRP Technology are useful in many areas of the diagnostic and biotech industry to increase sensitivity and to reduce the assay time. Our research partner SENOVA is prepared to assist our customers in specific product development by offering: Feasibility studies Reagent development Assay development and optimisation Custom conjugation Design and development Validation and Production ABICAP custom development SEV 3D-IF rapid test study feasibility SEV 3D-IF optimisation SEV 3D-IF validation PolyHRP custom conjugation Productnumber Product Quantity/Amount Price in SEV PolyHRP ELISA study (feasibility) SEV PolyHRP - pilot conjugation up to 10 mg SEV PolyHRP - conjugation This conjugation is based on the PolyHRP - pilot conjugation ( SEV) and can only be ordered additionally mg mg > 100 mg 340,00 / mg 310,00 / mg / mg
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