Predict Reactivity Note Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%)

Size: px
Start display at page:

Download "Predict Reactivity Note Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%)"

Transcription

1 Datasheet GeneTex, Inc : Toll Free GeneTex ( ) Fax: GeneTex International Corporation : Tel: Fax: Date : 2015/10/8 Catalog Number GTX Package:100 μl ( 5 ) ( 5 ) Product Name Full Name Product Description Background Host Clonality Clone Name Isotype Immunogen Antigen Species Species Reactivity Predicted Cross Reactivity species beta Actin antibody [GT5512] actin, beta Mouse Monoclonal antibody to beta Actin (actin, beta) This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] Mouse Monoclonal GT5512 IgG1 Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary. Human Human, Mouse, Drosophila, Rat, Zebrafish, Yeast, Goat, Rabbit Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine, Guinea pig Predict Reactivity Note Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%) Applications ICC/IF, WB Application Note Suggested dilution ICC/IF 1:100-1:1000* Western blot 1:1000-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher. Reference Positive Controls Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, NIH-3T3, mouse brain, PC-12, rat brain, drosophila, yeast, 30 hpf zebrafish, rabbit blood, goat blood, *L6, *Ishikawa, *U87-MG Predicted Target Size 42 Cellular Localization Conjugation Form Supplied Cytoplasm, cytoskeleton Unconjugated Liquid Purification Affinity purified by Protein G. Concentration Storage Buffer Storage Instruction Notes ResearchArea 1 mg/ml PBS Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Cell Biology > Cytoskeleton Signal Transduction > ECM/Cytoskeleton > Cytoskeleton Zebrafish > Validated Product

2 Application Reference 1. Zhong X (2015) Endocrinology Chang WC (2015) Food Res Int 3. Huang SY (2014) PLoS One e Noomhorm N (2014) J Pharmacol Sci Alberio T (2014) Biochim Biophys Acta A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg 293T whole cell lysate/extract D. 30 µg A431 whole cell lysate/extract E. 30 µg HeLa whole cell lysate/extract F. 30 µg HepG2 whole cell lysate/extract G. 30 µg H1299 whole cell lysate/extract H. 30 µg HCT116 whole cell lysate/extract I. 30 µg MCF-7 whole cell lysate/extract J. 30 µg NT2D1 whole cell lysate/extract K. 30 µg PC-3 whole cell lysate/extract L. 30 µg U87-MG whole cell lysate/extract Beta Actin antibody [GT5412] (GTX629630) dilution: 1:20000 A. 30 µg 293T whole cell lysate/extract

3 A. 30 µg 293T whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg mouse brain lysate/extract D. 30 µg PC-12 whole cell lysate/extract E. 30 µg rat brain lysate/extract Beta Actin antibody [GT5412] (GTX629630) dilution: 1:10000 A. 10 µg yeast lysate/extract beta Actin antibody [GT5412] (GTX629630) dilution: 1:1000 A. 30 µg drosophila lysate/extract Beta Actin antibody [GT5412] (GTX629630) dilution: 1:1000

4 A. 30 µg rabbit blood lysate/extract beta Actin antibody [GT5412] (GTX629630) dilution: 1:10000 A. 30 µg goat blood lysate/extract beta Actin antibody [GT5412] (GTX629630) dilution: 1:10000 GTX ICC/IF Image beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: beta Actin protein stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500. Blue: Hoechst staining.

5 A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract D. 1 µg 293T whole cell lysate/extract Beta Actin antibody [GT5412] (GTX629630) dilution: 1:10000 A. 30 µg 30 hpf zebrafish lysate/extract beta Actin antibody [GT5412] (GTX629630) dilution: 1:1000

Custom Antibody Services

Custom Antibody Services prosci-inc.com Custom Antibody Services High Performance Antibodies and More Broad Antibody Catalog Extensive Antibody Services CUSTOM ANTIBODY SERVICES Established in 1998, ProSci Incorporated is a leading

More information

CUSTOM ANTIBODIES. Fully customised services: rat and murine monoclonals, rat and rabbit polyclonals, antibody characterisation, antigen preparation

CUSTOM ANTIBODIES. Fully customised services: rat and murine monoclonals, rat and rabbit polyclonals, antibody characterisation, antigen preparation CUSTOM ANTIBODIES Highly competitive pricing without compromising quality. Rat monoclonal antibodies for the study of gene expression and proteomics in mice and in mouse models of human diseases available.

More information

Antibody Services. Best Guarantees in the Industry! Monoclonal Antibody Services. Polyclonal Antibody Services. Express Antibody TM Services

Antibody Services. Best Guarantees in the Industry! Monoclonal Antibody Services. Polyclonal Antibody Services. Express Antibody TM Services Antibody Services Best Guarantees in the Industry! Monoclonal Antibody Services Polyclonal Antibody Services Express Antibody TM Services Guaranteed Antibody Services Antibody Purification Services Your

More information

FAK phospho S732 Antibody, Rabbit Polyclonal Antibody

FAK phospho S732 Antibody, Rabbit Polyclonal Antibody GenWay Biotech, Inc. 6777 Nancy Ridge Drive San Diego, CA 92121 Tel: (858)458-0866 Fax: (858)458-0833 sales@genwaybio.com FAK phospho S732 Antibody, Rabbit Polyclonal Antibody Catalog Number: 18-272-197354

More information

Custom Antibodies Services. GeneCust Europe. GeneCust Europe

Custom Antibodies Services. GeneCust Europe. GeneCust Europe GeneCust Europe Laboratoire de Biotechnologie du Luxembourg S.A. 6 rue Dominique Lang L-3505 Dudelange Luxembourg Tél. : +352 27620411 Fax : +352 27620412 Email : info@genecust.com Web : www.genecust.com

More information

Geniron. Custom Antibody Services. Serum services Antibody Monoclonal. Purification Antibody Mono Y Genetic Immunization Genbody Polyclonal Antibody

Geniron. Custom Antibody Services. Serum services Antibody Monoclonal. Purification Antibody Mono Y Genetic Immunization Genbody Polyclonal Antibody Geniron Custom Antibody Services Serum services Antibody Monoclonal Purification Antibody Mono Y Genetic Immunization Genbody Polyclonal Antibody Geniron Poly Y WE PROVIDE OUR SERVICES TO With Expertise

More information

Antibody Production Price List

Antibody Production Price List Antibody Production Price List Presenting Insight Biotechnology s price list for custom polyclonal and monoclonal antibody production services. We are happy to tailor individual packages towards the specific

More information

Aviva Systems Biology

Aviva Systems Biology Aviva Custom Antibody Service and Price Mouse Monoclonal Antibody Service Package Number Description Package Contents Time Price Customer provides antigen protein $6,174 Monoclonal package1 (From protein

More information

Aviva Systems Biology

Aviva Systems Biology Aviva Custom Antibody Services and Prices Rabbit Polyclonal Antibody Service Package Number Description Package Contents Time Price Polyclonal package 1 (From protein to antiserum) Polyclonal package 2

More information

HuCAL Custom Monoclonal Antibodies

HuCAL Custom Monoclonal Antibodies HuCAL Custom Monoclonal Antibodies Highly Specific Monoclonal Antibodies in just 8 Weeks PROVEN, HIGHLY SPECIFIC, HIGH AFFINITY ANTIBODIES IN 8 WEEKS WITHOUT HuCAL PLATINUM IMMUNIZATION (Human Combinatorial

More information

HuCAL Custom Monoclonal Antibodies

HuCAL Custom Monoclonal Antibodies HuCAL Custom Monoclonal HuCAL Custom Monoclonal Antibodies Highly Specific, Recombinant Antibodies in 8 Weeks Highly Specific Monoclonal Antibodies in Just 8 Weeks HuCAL PLATINUM (Human Combinatorial Antibody

More information

General western blot protocol. Guidance for running an efficient and accurate experiment

General western blot protocol. Guidance for running an efficient and accurate experiment blot protocol Guidance for running an efficient and accurate experiment Contents Introduction Solution and reagents Sample lysis Sample preparation Loading and running the gel Antibody staining Useful

More information

Anti-ATF6 α antibody, mouse monoclonal (1-7)

Anti-ATF6 α antibody, mouse monoclonal (1-7) Anti-ATF6 α antibody, mouse monoclonal (1-7) 73-500 50 ug ATF6 (activating transcription factor 6) is an endoplasmic reticulum (ER) membrane-bound transcription factor activated in response to ER stress.

More information

Elabscience Biotechnology Co.,Ltd

Elabscience Biotechnology Co.,Ltd Biotechnology Co.,Ltd Your one-stop custom antibody service Antibody is a kind of protein that is the most widely used in the fields of biology and medicine. Antibodies, as the preparations for disease

More information

Chapter 2 Antibodies. Contents. Introduction

Chapter 2 Antibodies. Contents. Introduction Chapter 2 Antibodies Keywords Immunohistochemistry Antibody labeling Fluorescence microscopy Fluorescent immunocytochemistry Fluorescent immunohistochemistry Indirect immunocytochemistry Immunostaining

More information

3 months 1.5 months 1.5 months. 1 month

3 months 1.5 months 1.5 months. 1 month Rabbit monoclonal antibody (Mab) is secreted by the plasma B-cell of the rabbit. Traditional generation of rabbit Mab relies on a rabbit myeloma for B- cell fusion (

More information

THE His Tag Antibody, mab, Mouse

THE His Tag Antibody, mab, Mouse THE His Tag Antibody, mab, Mouse Cat. No. A00186 Technical Manual No. TM0243 Update date 01052011 I Description.... 1 II Key Features. 2 III Storage 2 IV Applications.... 2 V Examples - ELISA..... 2 VI

More information

POLYCLONAL ANTIBODY DEVELOPMENT

POLYCLONAL ANTIBODY DEVELOPMENT POLYCLONAL ANTIBODY DEVELOPMENT Abcore is committed to excellence, and to responding to the changing needs of our customers. We have developed polyclonal antibodies for biotechnology and pharmaceutical

More information

KMS-Specialist & Customized Biosimilar Service

KMS-Specialist & Customized Biosimilar Service KMS-Specialist & Customized Biosimilar Service 1. Polyclonal Antibody Development Service KMS offering a variety of Polyclonal Antibody Services to fit your research and production needs. we develop polyclonal

More information

Nitrotyrosine Western blot starter pack

Nitrotyrosine Western blot starter pack Nitrotyrosine Western blot starter pack Cat no. A010-513 A convenient reagent pack containing Badrilla s high integrity nitrotyrosine monoclonal antibody (clone 2E11) and associated positive and negative

More information

Chapter 18: Applications of Immunology

Chapter 18: Applications of Immunology Chapter 18: Applications of Immunology 1. Vaccinations 2. Monoclonal vs Polyclonal Ab 3. Diagnostic Immunology 1. Vaccinations What is Vaccination? A method of inducing artificial immunity by exposing

More information

SCIENCE WHERE YOU ARE

SCIENCE WHERE YOU ARE Newsletter. November 2013 SCIENCE WHERE YOU ARE Dealing with BioNordika includes a number of first class benefits: Next day delivery.... and some times even faster! Free delivery. Focus on your application.

More information

COMPANY INTRODUCTION. www.cusabio.com

COMPANY INTRODUCTION. www.cusabio.com www.cusabio.com COMPANY INTRODUCTION CUSABIO is a leading biotechnology company specialized in production of high quality products for gene, protein, antibody, ELISA kit, in vitro diagnostic raw material,

More information

Your partner in immunology

Your partner in immunology Your partner in immunology Expertise Expertise Reactivity Reactivity Quality Quality Advice Advice Who are we? Specialist of antibody engineering Covalab is a French biotechnology company, specialised

More information

Superior TrueMAB TM monoclonal antibodies for the recognition of proteins native epitopes

Superior TrueMAB TM monoclonal antibodies for the recognition of proteins native epitopes Superior TrueMAB TM monoclonal antibodies for the recognition of proteins native epitopes Outlines Brief introduction of OriGene s mission on gene-centric product solution. TrueMAB monoclonal antibody

More information

Antibody Services from GenScript

Antibody Services from GenScript Services from GenScript www.genscript.com GenScript USA Inc. 860 Centennial Ave., Piscataway, NJ 08854 USA Toll-Free: 1-877-436-7274 Fax: 1-732-210-0262 1-732-885-5878 Academic Services Pharmaceutical

More information

Monoclonal Antibodies, Brochure

Monoclonal Antibodies, Brochure Monoclonal Antibodies, Brochure Interest in any of the products, request or order them at Bio-Connect Diagnostics. Bio-Connect Diagnostics B.V. T NL +31 (0)26 326 44 60 T BE +32 (0)2 502 12 53 Begonialaan

More information

MAB Solut. MABSolys Génopole Campus 1 5 rue Henri Desbruères 91030 Evry Cedex. www.mabsolut.com. is involved at each stage of your project

MAB Solut. MABSolys Génopole Campus 1 5 rue Henri Desbruères 91030 Evry Cedex. www.mabsolut.com. is involved at each stage of your project Mabsolus-2015-UK:Mise en page 1 03/07/15 14:13 Page1 Services provider Department of MABSolys from conception to validation MAB Solut is involved at each stage of your project Creation of antibodies Production

More information

Custom polyclonal antibody production

Custom polyclonal antibody production EUROGENTEC 2008 Catalogue > www.eurogentec.com > order@eurogentec.com Custom polyclonal antibody production 231 General overview 232 Selecting a polyclonal programme 233 Peptide antibodies versus protein

More information

Huntingtin Antibody AbVantage Pack

Huntingtin Antibody AbVantage Pack Huntingtin Antibody AbVantage Pack Produced in Rabbit Catalog No. A3-034A NP_00202.4 CONTENTS Rabbit anti-huntingtin A302-82A Rabbit anti-huntingtin A302-83A WB, IP REACTIVITY STORAGE / SHELF LIFE 2-8

More information

Pure-IP Western Blot Detection Kit

Pure-IP Western Blot Detection Kit Product Manual Pure-IP Western Blot Detection Kit Catalog Number PRB-5002 20 blots FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction The technique of immunoprecipitation (IP) is used

More information

Bio-manufacturing of antigens, antibodies and bioresearch materials

Bio-manufacturing of antigens, antibodies and bioresearch materials Bio-manufacturing of antigens, antibodies and bioresearch materials Foetal Bovine Serum (FBS) Human AB Serum Chick Embryo Extract (CEE) Custom polyclonal antibody production Custom monoclonal antibody

More information

Affi-Prep Protein A Matrix Instruction Manual

Affi-Prep Protein A Matrix Instruction Manual Affi-Prep Protein A Matrix Instruction Manual Catalog Numbers 156-0005 156-0006 Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547 LIT-230 Rev B Table of Contents Section 1 Introduction...1

More information

Questions related to peptide antibodies

Questions related to peptide antibodies www.lifetechindia.com Some Commonly Asked Questions about custom antibody production (Click over the Q# to see details) Questions related to protein antigens and general information Q1 How much antigen

More information

ELITE Custom Antibody Services

ELITE Custom Antibody Services ELITE Custom Antibody Services ELITE Custom Antibody Services Experience, confidence, and understanding As a manufacturer and service provider, we have the experience, confidence, and understanding to

More information

Custom Polyclonal Anti-Peptide Antibody, Brochure

Custom Polyclonal Anti-Peptide Antibody, Brochure Custom Polyclonal Anti-Peptide Antibody, Brochure Interest in any of the products, request or order them at Bio-Connect. Bio-Connect B.V. T NL +31 (0)26 326 44 50 T BE +32 (0)2 503 03 48 Begonialaan 3a

More information

Antibody Services from GenScript, Brochure

Antibody Services from GenScript, Brochure Services from GenScript, Brochure Interest in any of the products, request or order them at Bio-Connect. Bio-Connect B.V. T NL +31 (0)26 326 44 50 T BE +32 (0)2 503 03 48 Begonialaan 3a F NL +31 (0)26

More information

Product Datasheet. DARC Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 5

Product Datasheet. DARC Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 5 Product Datasheet DARC Antibody NB100-2421 Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Publications: 5 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nb100-2421

More information

Antibodies Against Fluorescent Proteins

Antibodies Against Fluorescent Proteins Antibodies Against Fluorescent Proteins Section C - A collection of antibodies for the detection of Evrogen Fluorescent Proteins in different applications. Contents Overview............................................

More information

Custom Antibodies. Animals Chicken, Goat, Guinea Pig, Mouse, Rat, Rabbit, etc. Antibody Identification Dot blot ELISA IHC Western blot

Custom Antibodies. Animals Chicken, Goat, Guinea Pig, Mouse, Rat, Rabbit, etc. Antibody Identification Dot blot ELISA IHC Western blot Custom Antibodies aapptec provides custom peptides and immunology products with high quality and fast delivery at an affordable price to research scientists worldwide. aapptec has unparalleled experience

More information

Methionine Sulfoxide Immunoblotting Kit

Methionine Sulfoxide Immunoblotting Kit Methionine Sulfoxide Immunoblotting Kit Item No. 600160 Customer Service 800.364.9897 * Technical Support 888.526.5351 www.caymanchem.com TABLE OF CONTENTS GENERAL INFORMATION 3 Materials Supplied 4 Precautions

More information

QED Bioscience Inc. Revision 05/04/11 ADVANCED ANTIBODY TECHNOLOGIES

QED Bioscience Inc. Revision 05/04/11 ADVANCED ANTIBODY TECHNOLOGIES CUSTOM POLYCLONAL ANTIBODY DEVELOPMENT QED is committed to excellence across all facets of our operations, and to responding to the ever-changing needs of our customers. We have been developing monoclonal

More information

IMMUNOPRECIPITATION (IP) PROTOCOL

IMMUNOPRECIPITATION (IP) PROTOCOL IMMUNOPRECIPITATION (IP) PROTOCOL Immunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of interest is incubated with a cell extract so that the antibody

More information

Optimal Conditions for F(ab ) 2 Antibody Fragment Production from Mouse IgG2a

Optimal Conditions for F(ab ) 2 Antibody Fragment Production from Mouse IgG2a Optimal Conditions for F(ab ) 2 Antibody Fragment Production from Mouse IgG2a Ryan S. Stowers, 1 Jacqueline A. Callihan, 2 James D. Bryers 2 1 Department of Bioengineering, Clemson University, Clemson,

More information

Bio-Reagents Gene synthesis Peptide Synthesis Protein Expression Antibody Production. Life Science Products and Services

Bio-Reagents Gene synthesis Peptide Synthesis Protein Expression Antibody Production. Life Science Products and Services Bio-Reagents Gene synthesis Peptide Synthesis Protein Expression Antibody Production Life Science Products and Services Since 2002, Biomatik has provided worldwide researchers in life science discovery

More information

From Biology to Discovery

From Biology to Discovery POLYCLONAL ANTIBODY PRODUCTION SERVICES Abbiotec offers custom polyclonal antibody production services to the scientific community to accelerate a project From Biology to Discovery. When the antibody for

More information

Cellular Implants as mini-factories for the production of therapeutic antibodies against Altzheimer s disease. rapport research week Jonas Schiffmann

Cellular Implants as mini-factories for the production of therapeutic antibodies against Altzheimer s disease. rapport research week Jonas Schiffmann Cellular Implants as mini-factories for the production of therapeutic antibodies against Altzheimer s disease rapport research week Jonas Schiffmann Supported by Aurélien Lathuilière, Bernard Schneider

More information

GenScript Antibody Services

GenScript Antibody Services GenScript Antibody Services Scientific experts, innovative technologies, proven performance We specialize in custom antibody production to empower your research Custom Polyclonal Antibody Production Custom

More information

FluoProbes dyes. FluoProbes dyes directly conjugated to secondary antibodies

FluoProbes dyes. FluoProbes dyes directly conjugated to secondary antibodies FluoProbes dyes Interchim's FluoProbes dyes exhibit optimal fluorescence intensity combined with excellent photostability. Activated dyes are available independent (please request information), conjugated

More information

Integrated Protein Services

Integrated Protein Services Integrated Protein Services Custom protein expression & purification Last date of revision June 2015 Version DC04-0013 www.iba-lifesciences.com Expression strategy The first step in the recombinant protein

More information

GenScript Antibody Services

GenScript Antibody Services GenScript Antibody Services Scientific experts, innovative technologies, proven performance We specialize in custom antibody production to empower your research Custom Polyclonal Antibody Production Custom

More information

Recombinant Antibody Fragments, Brochure

Recombinant Antibody Fragments, Brochure Recombinant Antibody Fragments, Brochure Interest in any of the products, request or order them at Bio-Connect Diagnostics. Bio-Connect Diagnostics B.. T NL +31 (0)26 326 44 60 T BE +32 (0)2 502 12 53

More information

Antibodies. Thomas Boenisch, MS, and Jim Hudson, PhD

Antibodies. Thomas Boenisch, MS, and Jim Hudson, PhD Thomas Boenisch, MS, and Jim Hudson, PhD Immunoglobulins The vertebrate immune system has the amazing ability to create millions of different antibodies with different specificities. It is their ability

More information

MAKING AN EVOLUTIONARY TREE

MAKING AN EVOLUTIONARY TREE Student manual MAKING AN EVOLUTIONARY TREE THEORY The relationship between different species can be derived from different information sources. The connection between species may turn out by similarities

More information

APPLICATION FOCUS. Application Solutions for Western Blotting

APPLICATION FOCUS. Application Solutions for Western Blotting APPLICATION FOCUS Application Solutions for Western Blotting WESTERN BLOTTING Companion Products Solutions for consistently better blotting. Companion products from Cell Signaling Technology (CST) are

More information

Integrated Protein Services

Integrated Protein Services Integrated Protein Services Custom protein expression & purification Version DC04-0012 Expression strategy The first step in the recombinant protein generation process is to design an appropriate expression

More information

Dot Blot Analysis. Teacher s Guidebook. (Cat. # BE 502) think proteins! think G-Biosciences www.gbiosciences.com

Dot Blot Analysis. Teacher s Guidebook. (Cat. # BE 502) think proteins! think G-Biosciences www.gbiosciences.com PR110 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Dot Blot Analysis Teacher s Guidebook (Cat. # BE 502) think proteins! think G-Biosciences

More information

PROTOCOL. Immunostaining for Flow Cytometry. Background. Materials and equipment required.

PROTOCOL. Immunostaining for Flow Cytometry. Background. Materials and equipment required. PROTOCOL Immunostaining for Flow Cytometry 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 Rev.0 Background The combination of single cell analysis using flow cytometry and the specificity of antibody-based

More information

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies A Brief Review of Antibody Structure A Brief Review of Antibody Structure The basic antibody is a dimer of dimer (2 heavy chain-light chain pairs) composed of repeats of a single structural unit known

More information

Secondary Antibodies and Conjugates

Secondary Antibodies and Conjugates biodescriptor biomolecules Full image -- Remove color from text box when placing image Secondary Antibodies and Conjugates Initiative Descriptor Primary Cover Header Popular Reagents for Quality Secondary

More information

The immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small

The immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small The immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small molecules that can elicit an immune response when linked

More information

Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel

Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel Catalog Number SC025 Reagents for the identification of human/mouse/rat neural progenitor cells. This package insert must be read in its entirety

More information

WESTERN BLOT DETECTION KIT Buffers and detection reagents for up to ten 10 x 10 cm 2 blots. Fluorescent detection via: Goat anti-mouse SureLight P3

WESTERN BLOT DETECTION KIT Buffers and detection reagents for up to ten 10 x 10 cm 2 blots. Fluorescent detection via: Goat anti-mouse SureLight P3 WESTERN BLOT DETECTION KIT Buffers and detection reagents for up to ten 10 x 10 cm 2 blots Fluorescent detection via: Goat anti-mouse SureLight P3 Cat. #: WK-P112 6440 Dobbin Road, Suite D Phone (443)

More information

Advanced BioDesign Outlines Solutions. Antibody Overview. by Advanced BioDesign. Project Start. Immunogenicity. Selecting Your Antigen

Advanced BioDesign Outlines Solutions. Antibody Overview. by Advanced BioDesign. Project Start. Immunogenicity. Selecting Your Antigen Advanced BioDesign Outlines Solutions by Advanced BioDesign Antibody Overview Launching an immunisation programme is an important experimental step that needs care. With Advanced BioDesign, you may develop

More information

Opposition against European Patent EP 1 456 346 B1

Opposition against European Patent EP 1 456 346 B1 Opposition against European Patent EP 1 456 346 B1 Title: NOVEL ECDYSONE RECEPTOR/INVERTEBRATE RETINOID X RECEPTOR-BASED INDUCIBLE GENE EXPRESSION SYSTEM Application number: 02714955.8 Proprietor: Intrexon

More information

Antibody Cross-Reactivity Chart

Antibody Cross-Reactivity Chart Page 1 of 6 CD2 RPA-2.10 Human Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Swine (Pig, Porcine) CD3 SK7 Human Chimpanzee CD3 UCHT1 Human Chimpanzee CD4 OKT4 Human Chimpanzee,

More information

Fluorescein Isothiocyanate (FITC)- conjugated Antibodies

Fluorescein Isothiocyanate (FITC)- conjugated Antibodies USER GUIDE Fluorescein Isothiocyanate (FITC)- conjugated Antibodies Catalog Numbers R933-25, R953-25, R963-25 Document Part Number 25-0376 Publication Number MAN0000194 Revision 2.0 For Research Use Only.

More information

LYME DISEASE. 2.5M specimen tests per year. 97% accuracy with Rockland tools

LYME DISEASE. 2.5M specimen tests per year. 97% accuracy with Rockland tools LYME DISEASE The current situation & our solutions Lateral Flow Data Proof of Concept Studies Core Technology Sequence Analysis & Protein Expression The most common vector-borne illness in the United States

More information

1.Gene Synthesis. 2.Peptide & Phospho-P. Assembly PCR. Design & Synthesis. Advantages. Specifications. Advantages

1.Gene Synthesis. 2.Peptide & Phospho-P. Assembly PCR. Design & Synthesis. Advantages. Specifications. Advantages 1.Gene Synthesis Assembly PCR Looking for a cdna for your research but could not fish out the gene through traditional cloning methods or a supplier? Abnova provides a gene synthesis service via assembly

More information

STANDARD OPERATING PROCEDURE

STANDARD OPERATING PROCEDURE STANDARD OPERATING PROCEDURE Title: Evaluation using Western Blot SOP#: M-103 Version #: 1 Author: R. Saul Date Approved: Feb. 5, 2009 Date Modified: 1. PURPOSE The purpose of this document is to describe

More information

Custom Antibody Services

Custom Antibody Services Custom Antibody Services Custom service offerings DNA sequence Plasmid Peptide Structure Protein Peptide Small molecule Cells Spleen Lymphocytes Antigen Preparation Immunization Fusion & Subcloning Expansion

More information

Zenon Labeling Technology A New Approach to Immunolabeling from Molecular Probes

Zenon Labeling Technology A New Approach to Immunolabeling from Molecular Probes Zenon Labeling Technology A New Approach to Immunolabeling from Molecular Probes Applications for Flow Cytometry and Imaging A New Approach to Immunolabeling Zenon labeling technology provides a versatile,

More information

Biotinylated Secondary Antibodies

Biotinylated Secondary Antibodies Biotinylated Secondary Antibodies Anti-Goat ABB-02-01 Biotin Labeled Rabbit Anti-Goat IgG (H+L) Antibody $44.00 Affinity purified polyclonal antibody to Goat IgG, heavy and light chains (whole IgG) made

More information

Chapter 3.2» Custom Monoclonal

Chapter 3.2» Custom Monoclonal 198 3 3.2 Custom Monoclonal 199 Mouse monoclonal antibody development Chapter 3.2» Custom Monoclonal 200 In vitro monoclonals expression service 201 Mouse monoclonal antibody additional services 202 Magnetic

More information

P R O D U C T S CATALOG 2011-2012

P R O D U C T S CATALOG 2011-2012 P R O D U C T S CATALOG 2011-2012 INSTITUTE FOR THE APPLICATION OF NUCLEAR ENERGY - INEP Banatska 31b 11080 Zemun Belgrade Serbia Tel: (+381 11) 2619 252, 2618 696, 2199 949 Fax: (+381 11) 2618 724 www.inep.co.rs

More information

Odyssey Imaging Systems

Odyssey Imaging Systems Odyssey Imaging Systems Good Westerns Gone Bad: Tips to Make Your NIR Western Blot Great Published December 2008. Revised June 2010. The most recent version of this document is posted at http://biosupport.licor.com/support

More information

Mouse GLP-2 ELISA. For quantitative determination of mouse GLP-2 in serum or plasma samples.

Mouse GLP-2 ELISA. For quantitative determination of mouse GLP-2 in serum or plasma samples. Mouse GLP-2 ELISA For quantitative determination of mouse GLP-2 in serum or plasma samples. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 48-GP2MS-E01 Size: 96 Wells Version:

More information

In-Cell ELISA Support Pack for Suspension Cells

In-Cell ELISA Support Pack for Suspension Cells ab111541 In-Cell ELISA Support Pack for Suspension Cells Instructions for Use For use with suspension or apoptotic/detaching cells This product is for research use only and is not intended for in vitro

More information

INSTRUCTION Probemaker

INSTRUCTION Probemaker INSTRUCTION Probemaker Instructions for Duolink In Situ Probemaker PLUS (Art. no. 92009-0020) and Duolink In Situ Probemaker MINUS (Art. no. 92010-0020) Table of content 1. Introduction 4 2. Applications

More information

Protein extraction from Tissues and Cultured Cells using Bioruptor Standard & Plus

Protein extraction from Tissues and Cultured Cells using Bioruptor Standard & Plus Protein extraction from Tissues and Cultured Cells using Bioruptor Standard & Plus Introduction Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical

More information

Antibody Purification and Labeling

Antibody Purification and Labeling 5 Antibody Purification and Labeling 5.1 Antibody Purification 65 Magne Protein A Beads and Magne Protein G Beads 67 5.2 Antibody Labeling 69 phab Amine and Thiol Reactive Dyes 71 63 Discover Reliable

More information

Compromise Elsewhere Protocols. Western Blotting Methods. 800-656-ROCK www.rockland-inc.com info@rockland-inc.com 1 of 11

Compromise Elsewhere Protocols. Western Blotting Methods. 800-656-ROCK www.rockland-inc.com info@rockland-inc.com 1 of 11 Compromise Elsewhere Protocols Western Blotting Methods info@rockland-inc.com 1 of 11 Odyssey Western Blotting Protocols Odyssey reagents: Additional reagents needed: IR-labeled secondary antibodies Odyssey

More information

Really Good Antibodies. Secondary Antibodies. Over 670 Anti-Mouse, Rabbit, Goat and Rat Secondary Antibodies. bethyl.com

Really Good Antibodies. Secondary Antibodies. Over 670 Anti-Mouse, Rabbit, Goat and Rat Secondary Antibodies. bethyl.com Really Good Antibodies Secondary Antibodies Over 670 Anti-Mouse, Rabbit, Goat and Rat Secondary Antibodies bethyl.com Bethyl Laboratories, Inc. has been dedicated to supporting scientific discovery through

More information

Chromatin Immunoprecipitation (ChIP)

Chromatin Immunoprecipitation (ChIP) Chromatin Immunoprecipitation (ChIP) Day 1 A) DNA shearing 1. Samples Dissect tissue (One Mouse OBs) of interest and transfer to an eppendorf containing 0.5 ml of dissecting media (on ice) or PBS but without

More information

Making the switch to a safer CAR-T cell therapy

Making the switch to a safer CAR-T cell therapy Making the switch to a safer CAR-T cell therapy HaemaLogiX 2015 Technical Journal Club May 24 th 2016 Christina Müller - chimeric antigen receptor = CAR - CAR T cells are generated by lentiviral transduction

More information

perfectprotein Antibodies ntelechon

perfectprotein Antibodies ntelechon Antibodies ntelechon Milstein and Köhler: The hybridoma technology December 1974, Cambridge: Georges J.F. Köhler, a postdoctorial fellow of César Milstein, had given eternal life to normal antibody producing

More information

Genes to Proteins to Antibodies

Genes to Proteins to Antibodies Genes to Proteins to Antibodies About Us Fusion Antibodies is a CRO established in 2001 as a spin-out from Queen s University Belfast. The company building is situated in a charming area of Springbank

More information

Guide to Purification of Polyclonal Antibodies

Guide to Purification of Polyclonal Antibodies Guide to Purification of Polyclonal Antibodies When choosing a polyclonal antibody, either as a primary or secondary antibody in an immunoassay, researchers are often inundated with an array of antibody

More information

Custom Antibodies & Recombinant Proteins

Custom Antibodies & Recombinant Proteins Custom Antibodies & Recombinant Proteins INTRODUCTION Custom services to meet your research and development requirements Improvements in health, medicine and diagnostics over the past century can be largely

More information

Chapter 3.1» Custom Polyclonal Antibodies

Chapter 3.1» Custom Polyclonal Antibodies 180 3 3.1 Custom Polyclonal Chapter 3.1» Custom Polyclonal Antibodies Antibodies 181 General overview 182 Choosing your parameters 184 Selecting your animal host 185 Selecting your immunisation protocol

More information

Towards Well-Defined ADCs (Antibody Drug Conjugates)

Towards Well-Defined ADCs (Antibody Drug Conjugates) Towards Well-Defined ADCs (Antibody Drug Conjugates) Next generation approach June 10, 2015 Dr. Yong Zu Kim, CEO and President LegoChem Biosciences, Inc. Antibody Drug Conjugates ADC binds to Antigen Endocytosis

More information

High Resolution Epitope Mapping of Human Autoimmune Sera against Antigens CENPA and KDM6B. PEPperPRINT GmbH Heidelberg, 06/2014

High Resolution Epitope Mapping of Human Autoimmune Sera against Antigens CENPA and KDM6B. PEPperPRINT GmbH Heidelberg, 06/2014 High Resolution Epitope Mapping of Human Autoimmune Sera against Antigens CENPA and KDM6B PEPperPRINT GmbH Heidelberg, 06/2014 Introduction This report describes the epitope mapping of autoimmune sera

More information

Proteomics Research with BIOCHAIN

Proteomics Research with BIOCHAIN Proteomics Research with IOCHIN Protein Extraction CNMCS Compartmental Protein Extraction Kit Cytoplasmic, Nuclear, Membrane, and Cytoskeleton Protein One kit isolates four different proteins sequentially

More information

MagExtractor -Genome-

MagExtractor -Genome- Instruction manual MagExtractor-Genome-0810 F0981K MagExtractor -Genome- NPK-101 100 preparations Store at 4 C Contents [1] Introduction [2] Components [3] Materials required [4] Protocol 1. Purification

More information

Hypoxyprobe -1 Plus Kit Kit contents:

Hypoxyprobe -1 Plus Kit Kit contents: Updated 2015 1 PRODUCT INSERT Hypoxyprobe, Inc 121 Middlesex Turnpike Burlington, MA 01803 USA www.hypoxyprobe.com Hypoxyprobe -1 Plus Kit Kit contents: Solid pimonidazole HCl (Hypoxyprobe -1) FITC conjugated

More information

Western BLoT Immuno Booster

Western BLoT Immuno Booster Cat. # T7111A For Research Use Western BLoT Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Storage... 3 IV. Materials Required but Not Provided... 3 V. Precautions... 3 VI.

More information

SCANTIBODIES Laboratory, Inc. Contract Monoclonal Antibody Production

SCANTIBODIES Laboratory, Inc. Contract Monoclonal Antibody Production A Technical Publication of SCANTIBODIES Laboratory, Inc. Volume 1 Number 4 9336 Abraham Way Santee, CA 92071 USA (619) 258-9300 fax (619) 258-9366 www.scantibodies.com SCANTIBODIES Laboratory, Inc. Contract

More information

ab185915 Protein Sumoylation Assay Ultra Kit

ab185915 Protein Sumoylation Assay Ultra Kit ab185915 Protein Sumoylation Assay Ultra Kit Instructions for Use For the measuring in vivo protein sumoylation in various samples This product is for research use only and is not intended for diagnostic

More information

GUIDELINES FOR THE PRODUCTION OF ANTIBODIES IN LABORATORY ANIMALS

GUIDELINES FOR THE PRODUCTION OF ANTIBODIES IN LABORATORY ANIMALS GUIDELINES FOR THE PRODUCTION OF ANTIBODIES IN LABORATORY ANIMALS Table of Contents 1. Purpose 2. Choice of Species and Strain 3. Immunizing Antigen 4. Procedures for Polyclonal Antibody Production 5.

More information

Designing Assays for the TaqMan Protein Assay

Designing Assays for the TaqMan Protein Assay Designing Assays for the TaqMan Protein Assay Antibody Selection...2 Assay Design...2 Antibody Search...3 Biotinylated Antibodies...3 Working with pre-biotinylated antibodies...4 Working with non-biotinylated

More information