Normal values of IGF1 and IGFBP3. Kučera R., Vrzalová J., Fuchsová R., Topolčan O., Tichopád A.

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1 Normal values of IGF1 and IGFBP3 Kučera R., Vrzalová J., Fuchsová R., Topolčan O., Tichopád A.

2 Agenda of the presentation IGF1 and IGFBP3 basic characteristic Why normal values Groups of the persons and methods Normal values of IGF1 Normal values of IGFBP3 IGF1/IGFBP3 ratio Conclusion

3 Agenda of the presentation IGF1 and IGFBP3 basic characteristic Why normal values Groups of the persons and methods Normal values of IGF1 Normal values of IGFBP3 IGF1/IGFBP3 ratio Conclusion

4 IGF system IGFs IGF1, IGF2 IGFBPs IGFBP1...IGFBP6 Receptors for IGFs IGF1R, IGF2R, IR, hybrid receptor IR/IGF1R Signal pathway of IGFs PI3K/PKB, MAP kinases

5 Function of IGF1 and IGFBP3 Physiological function of IGF1 is absolutely essential for a healthy growth and development of the organism. Regulation of growth, metabolism, survival and differentiation of the cells in postnatal development. IGFBP3 is a most carrier protein of IGF1 and IGF2 in the blood Place of the synthesis of both analytes: liver Indication in routine practice: growth disorders Our topic: IGF1 and cancer

6 IGF1 Growth factor Molecular weight (Da) Amino acids number IGF Physiologic Function Regulation of growth, metabolism, cell survival and differentiation in postnatal development Half-life of free IGF1: <10min Half-life of IGF1/IGFBP3 complex: cca 12h

7 IGFBP3 Binding protein molecular weight (kda) IGFBP3 53 Binding affinity IGF1, IGF2 Occurrence serum Half-life of IGFBP3: 30min Half-life of IGF1(2)/IGFBP3 complex: cca 12h

8 Agenda of the presentation IGF1 and IGFBP3 basic characteristic Why normal values Groups of the persons Normal values of IGF1 Normal values of IGFBP3 IGF1/IGFBP3 ratio Conclusion

9 Why normal values part 1 Group of patients with cancer must be compared with a group of healthy persons IGF1 levels decline with age IGFBP3 levels decline with age We investigate cancer across the spectrum of the adult population (melanoma, prostate, colorectal, ovarian, breast ca)

10 Why normal values part 2 Serum IGF1 values are affected by various diseases Increased in diabetes and other disorders of the utilization of insulin and glucose metabolism IGF1 determination in cancer is a new indication Increased and decreased levels occur in tumors Increase and decrease is sometimes not very significant

11 Why normal values part 3 For obtaining correct results, it is necessary to compare the values measured in tumors with normal values of investigated population Small deviation of the investigated population from normal values may cause a wrong conclusion sufficient number of persons in each age group

12 Agenda of the presentation IGF1 and IGFBP3 basic characteristic Why normal values Groups of the persons and methods Normal values of IGF1 Normal values of IGFBP3 IGF1/IGFBP3 ratio Conclusion

13 Group of the persons blood donors euthyroid patients, without treatment - patients from Faculty Hospital Pilsen - we excluded patients with diabetes, high blood glucose, high insulin levels, patients after surgery, patients polymorbid and a large number of medications

14 IGF1 Group Age range (years) Number of persons (N) Number of men (N) Number of women (N) and more Total

15 IGFBP3 Group Age range (years) Number of persons (N) Number of men (N) Number of women (N) Total

16 Analytical and statistical methods IGF1 IRMA, Beckman Coulter, Immunotech, Marseille IGFBP3 IRMA, Beckman Coulter, Immunotech, Prague The SAS 9.2 (Statistical Analysis Software release 9.2) was used for all statistical analysis.

17 Agenda of the presentation IGF1 and IGFBP3 basic characteristic Why normal values Groups of the persons and methods Normal values of IGF1 Normal values of IGFBP3 IGF1/IGFBP3 ratio Conclusion

18 Normal values of IGF1 Age range (years) Number of persons (N) Median (ng/ml) Min Max (ng/ml) and more

19 Agenda of the presentation IGF1 and IGFBP3 basic characteristic Why normal values Groups of the persons and methods Normal values of IGF1 Normal values of IGFBP3 IGF1/IGFBP3 ratio Conclusion

20 Normal values of IGFBP3 Age range (years) Number of persons (N) Median (ng/ml) Min Max (ng/ml)

21 Agenda of the presentation IGF1 and IGFBP3 basic characteristic Why normal values Groups of the persons and methods Normal values of IGF1 Normal values of IGFBP3 IGF1/IGFBP3 ratio Conclusion

22 IGF1 / IGFBP3 ratio Many patients have the same level of IGF1 at very different levels of IGFBP3 and conversely Some authors evaluate the high levels of IGFBP3 as a protective factor and a positive prognostic factor with regard to tumor growth IGF1/IGFBP3 ratio gives a new view on risk and prognosis of cancer

23 Normal values of IGF1 / IGFBP3 ratio Age range (years) Number of persons (N) Median (ng/ml) Min Max (ng/ml)

24 Agenda of the presentation IGF1 and IGFBP3 basic characteristic Why normal values Groups of the persons and methods Normal values of IGF1 Normal values of IGFBP3 IGF1/IGFBP3 ratio Conclusion

25 Conclusion For obtaining correct results, it is necessary to compare the values measured in tumors with normal values of investigated population In our experience: 100 persons in each age group is a minimum number Ideal number: persons IGF1/IGFBP3 ratio is a new parameter in evaluation of risk and prognosis of cancer Our work will continue - adding persons into each age group up to number of 200 (300) - adding persons into age group up to 80 years