M A X I M I Z E S C I E N C E F O R L I F E 2008 CATALOG

Transcription

1 M A X I M I Z E S C I E N C E F O R L I F E 2008 CATALOG

2 T A B L E O F C O N T E N T S Food and Feed Safety Testing bioo scientific INTRODUCTION... 1 Food & FEED SAFETY OVERVIEW... 2 Food & FEED SAFETY PRODUCTS... 3 Food & FEED SAFETY PRODUCT LISTING... 4 Food & FEED SAFETY FEATURE PRODUCTS... 5 Research and Preclinical Testing RESEARCH & PRECLINICAL TESTING OVERVIEW... 6 RESEARCH & PRECLINICAL TESTING PRODUCTS... 7 CYTOKINE ASSAY LISTING... 8 ANTIBODY LISTING... 9 RESEARCH & PRECLINICAL FEATURE PRODUCTS...10 SERVICES...12 Customer reviews & contact information...13

3 Bioo Scientific Corporation (BIOO) is a biotechnology and health care company headquartered in Austin, Texas. Our focus is on maximizing science with a direct impact on life. Our products are being sold in over 40 countries under the trademarks MaxSignal and MaxSuppressor. M A X I M I Z E S C I E N C E F O R L I F E BIOO operates in four core areas 1. Food and Feed Safety Testing 2. Research and Preclinical Testing Products 3. BIOO Services 4. Drug Development 1 Our goal is to become a leader in the discovery, development, manufacture and marketing of effective and innovative testing, diagnosis and healthcare solutions that address the challenges facing patients and society in general. Our corporate values lie in the highest of ethical standards, with an ultimate focus on our customers. Bioo Scientific is dedicated to our customers needs. We offer a unique environment that is driving technology and growth in our industry. BIOO maintains a refreshing and respectful entrepreneurial environment that empowers our employees, collaborators and customers. We are creative thinkers and continue to add new products. We also promote collaborations to advance research and fuel new Bioo Scientific initiatives for the future.

4 B I O O F O O D & F E E D S A F E T Y O V E R V I E W Food and Feed Safety Testing M A X I M I Z E S C I E N C E F O R L I F E 2 As one of the leading companies in the field, we develop, manufacture and market a wide range of food and feed safety test products covering Antibiotics/Veterinary Drugs, Hormones, Microbiological tests, Mycotoxins, Phycotoxins, and Scombrotoxin (Histamine). A majority of these determinations can be done by either HPLC, LC-MS or GC-MS; however, these traditional methods usually require very expensive preparation procedures, longer processing time, experienced technicians or radioactive materials. BIOO ELISA kits, on the other hand, provide a simple, rapid, sensitive and cost-effective screening method. BIOO MaxSignal TM ELISA test kits are developed and produced with BIOO proprietary antibodies and reagents to achieve high sensitivity and low detection limits. Our rapid, high recovery and cost-effective extraction methods are heavily tested using real samples from different regions of the world and constantly validated by world-wide reference labs. A BIOO ELISA kit contains one 96-well plate (8 wells x 12 strips) and reagents, including standards and buffers, required to perform the test. It has the capacity for 96 determinations or testing of 42 samples in duplicate. The shelf life is 12 months when the kit is properly stored. Procedures for sample extraction are described in detail in the manual. Antibiotics / Veterinary Drugs MaxSignal Antibiotics / Veterinary Drugs Test Kits are designed for the quantitative analysis of veterinary drug residues such as chloramphenicol (CAP), crystal violet/lcv, diazepam, enrofloxacin, fluoroquinolone, furaltadone (AMOZ), furazolidone (AOZ), gentamicin, malachite green/lmg, streptomycin, sulfamethazine, tylosin and other drug residues in animal feed, crab, eggs, fish, honey, meat, milk, milk powder, serum, shrimp, urine and other matrices. Hormones MaxSignal Hormone ELISA Test Kits are designed for the quantitative analysis of beta-agonists such as bitolterol, carbuterol, cimaterol, clenbuterol, diethylstibestrol (DES), ractopamine, salbutamol, zilpaterol and other hormones in animal tissue, animal feed, urine and other matrices.

5 Microbiological Tests Phycotoxins Phycotoxins are marine biotoxins that accumulate in fish and shellfish from their diet algae. There are three different types of phycotoxins: PSP (paralytic shellfish poisoning), DSP (diarrhetic shellfish poison) and ASP (amnestic shellfish poison). The consumption of phycotoxin-contaminated food can lead to serious health problems that can be recognized by the following symptoms: damaged nervous system, damaged digestive system and memory loss. Toxincontaminated mussels can be found worldwide in the summer time. Phycotoxins-residues can be determined by either HPLC, LC-MS or GC-MS. However, BIOO enzyme immunoassays (ELISA) have considerable advantages over conventional methods. Bacteria exist in a range of environments but impose danger to health when found in food and feedstuffs. Food products and their raw materials such as poultry meat, fish, shrimp, pork, and eggs can provide the perfect growth medium for microbes. For example, when Salmonella contaminated food is ingested it may result in the disease known as Salmonellosis. Clinical symptoms include vomiting, diarrhea and nausea; this is referred to as gastroenteritis and in severe cases may result in death. The detection of bacteria in food samples by conventional microbiological techniques is often difficult, laborious and time-consuming. Several days are required to culture the bacteria from a sample followed by a final isolation and identification by serological and biochemical techniques. BIOO enzyme immunoassays (ELISA), however, offer a faster method of identification as compared with traditional techniques. Mycotoxins Mycotoxin is a toxin produced by fungi, which includes mushrooms, molds and yeasts. It is a secondary fungal metabolite that induces a variety of toxic and carcinogenic effects when food contaminated with the toxin is ingested. There are two major kinds of fungi producing mycotoxins. One is Fusarium species, which generate mycotoxins directly on agricultural crops, such as DON, Zearalenone, T-2 Toxin and Fumonisin, predominantly reach the food chain through cereals and cereal products. The other important group of fungi (e.g. Aspergillus and Penicillium species) occur during inadequate storage areas. The corresponding mycotoxins, such as Aflatoxins and Ochratoxin A, are found in incorrectly stored crop products or in food products directly. USFDA and EU have set up a range of mycotoxins permitted in food and animal feed. Scombrotoxin (Histamine) Certain bacteria produce the enzyme histidine decarboxylase during growth. This enzyme breaks down free histidine, a naturally occurring chemical that is present in large quantities in some fish (Scombridae, such as tuna, kahawai, mackerel, bonito and butterfly kingfish). This results in the formation of histamine and other amines. Histamine fish poisoning (HFP) is frequently encountered if dead fish remain in set nets during warm sea temperatures, the fish is improperly refrigerated or when refrigeration is delayed. The symptoms of HFP mimic those of an IgE-mediated food allergy: nausea, vomiting, diarrhea, hives, itching, red rash (flushing), and hypotension. The MaxSignal TM Histamine Enzymatic Assay is an efficient screening tool for the detection of histamine in seafood samples. (See page 5 for details.) 3 Food and Feed Safety Testing

6 Food and Feed Safety Testing 4 B I O O F O O D & F E E D S A F E T Y P R O D U C T S CAT. # ANALYte format SAMPLE TYPE 1 standards (ppb) lod* (ppb) 1008 Ractopamine elisa Animal Feed, Kidney, Liver, Meat, Milk, Urine 0 / 0.5 / 1.0 / 5.0 / 10 / Beta-agonist elisa Animal Feed, Meat, Tissue, Urine 0 / 0.05 / 0.15 / 0.5 / 1.5 / ** 1010 Diazepam elisa Animal Feed, Kidney, Liver, Meat, Milk, Serum, Urine 0 / 0.01 / 0.05 / 0.1 / 0.5 / Sulfamethazine ELISA Animal Feed, Honey, Kidney, Meat, Milk, Milk Powder, serum, Urine 0 / 0.25 / 0.5 / 1.0 / 5.0 / Diethylstibestrol (DES) ELISA Animal Feed, Fish, Meat, Urine 0 / 0.15 / 0.5 / 1.5 / 4.5 / Chloramphenicol (CAP) ELISA Animal Feed, Crab, Eggs, Fish, Honey, Meat, Milk, milk Powder, Serum, Shrimp, Urine 0 / 0.05 / 0.15 / 0.5 / 1.5 / Streptomycin elisa Animal Feed, Honey, Kidney, Liver, Meat, Milk, milk Powder, Serum, Urine 0 / 0.5 / 1.0 / 2.5 / 5.0 / Furazolidone (AOZ) ELISA eggs, Fish, Honey, Meat, Serum, Shrimp, Urine, Milk 0 / 0.05 / 0.15 / 0.5 / 1.5 / Enrofloxacin elisa Animal Feed, Fish, Kidney, Liver, Meat, Milk, Shrimp, Urine 0 / 0.1 / 0.25 / 0.5 / 1.0 / Malachite Green SPS 2 Water Sample 0 / 1.0 / 2.5 / 5.0 / 10 / 25 ppm Malachite Green/LMG ELISA Animal Feed, Fish, Shrimp, Water Sample 0 / 0.05 / 0.15 / 0.5 / 1.5 / Furaltadone (AMOZ) ELISA eggs, Fish, Honey, Meat, Serum, Shrimp, Urine, Milk 0 / 0.05 / 0.2 / 0.8 / 3.2 / Clenbuterol elisa Animal Feed, Meat, Tissue, Urine 0 / 0.05 / 0.15 / 0.5 / 1.5 / Salbutamol elisa Animal Feed, Meat, Tissue, Urine, Serum 0 / 0.05 / 0.15 / 0.5 / 1.5 / Total Antibiotics (in Milk) Microbial growth Milk Positive/Negative Controls 4.0*** 1024 Fluoroquinolone ELISA Animal Feed, Fish, Kidney, Liver, Meat, Milk, Shrimp, Urine 0 / 0.1 / 0.25 / 0.5 / 1.0 / Cimaterol elisa Animal Feed, Meat, Tissue, Urine, Serum 0 / 0.25 / 1.0 / 5.0 / 20 / Tylosin elisa Honey, Milk, Tissue, Urine 0 / 1.0 / 5.0 / 10 / 25 / Gentamicin elisa milk, Serum, Tissue, Urine 0 / 0.25 / 0.75 / 1.5 / 3.0 / Total Antibiotics (in Honey) Microbial growth Honey Positive/Negative Controls 4.0*** 1029 Crystal Violet/LCV ELISA Animal Feed, Fish, Shrimp, Water Sample 0 / 0.05 / 0.15 / 0.5 / 1.5 / Total Aflatoxin elisa feed, Dried Meat/Fish, Nuts, Spices 0 / / / 0.15 / 0.3 / Zilpaterol elisa Animal Feed, Meat, Tissue, Urine, Serum 0 / 0.1 / 0.15 / 0.3 / 0.4 / Histamine enzymatic Assay Fish/Seafood, Fish Meal, Milk, Wine 0 / 1.0 / 2.0 / 4.0 / 8.0 / 12 ppm 6.0 ppm 1033 Sulfadiazine elisa Animal Feed, Honey, Kidney, Meat, Milk, milk Powder, Serum, Urine 0 / 1.0 / 2.0 / 5.0 / 10 / Saxitoxin (PSP) ELISA fish/shrimp, Mussels, Water 0 / 0.02 / 0.04 / 0.08 / 0.16 / Zearalenone elisa feed, Cereal, Dried Meat/Fish, Milk, Seed 0 / 0.1 / 0.25 / 0.5 / 1.5 / Ochratoxin A elisa feed, Cereal, Dried Meat/Fish, Milk, Seed 0 / 0.15 / 0.45 / 1.35 / 4.05 / T2 elisa feed, Cereal, Dried Meat/Fish, Milk, Seed 0 / 1 / 5 / 10 / 25 / Fumonisin elisa feed, Cereal, Dried Meat/Fish, Milk, Seed 0 / 5 / 10 / 25 / 50 / Salmonella elisa feed, Fish, Meat Positive/Negative Controls 10^5 cells/ml 1040 E. coli O157 elisa feed, Fish, Meat, Vegetables Positive/Negative Controls 10^5 cells/ml *Detection limits vary in regard to different sample types; see product insert for details ** Detection limit on Clenbuterol; see product insert for detailed detection limits on other beta-agonists *** Detection limits on Penicillin and Ampicillin; see product insert for detailed detection limits on other antibiotics 1. Preparation protocols for samples other than above can be made available upon request 2. SPS: Solid Phase Separation

7 B I O O F O O D & F E E D S A F E T Y F E A T U R E P R O D U C T S MaxSignal Malachite Green/Leucomalachite Green (LMG) ELISA Test Kit (patent-pending) Catalog #: 1019 This product is the first commercially successful ELISA kit for detecting Malachite Green/Leucomalachite Green (LMG) in fish, shrimp, fish/shrimp feed or water samples of fish farms or fishponds. Malachite Green (Basic Green 4) has been widely used all over the world in the fish farming industry as a fungicide, ectoparasiticide and disinfectant. However, it has been shown that Malachite Green is highly cytotoxic to mammalian cells and also acts as a liver tumor-enhancing agent. This has led to a prohibition of Malachite Green for the treatment of fish and shrimp used for food production. To prevent Malachite Green residues from entering the food chain, both producers and government surveillance agencies need technologies and methods that provide rapid, accurate, and reliable results at the required sensitivity. Currently, Malachite Green residues are determined by either HPLC or LC-MS method with extremely expensive and time-consuming preparatory procedures. The MaxSignal TM Malachite Green/ LMG ELISA Test Kit provides a simple, rapid, sensitive and costeffective enzyme immunoassay (ELISA) screening method, which enables government agencies, seafood processors, as well as quality assurance organizations, to detect Malachite Green to as low as 0.1 ng/g or ppb level in fish and shrimp matrices. The unique features of the kit are: 1. High recovery (80-95%), and rapid extraction method for fish/shrimp or water samples. 2. Chemical conversion of LMG to Malachite Green before detection. Chromatic Malachite Green can be rapidly bioconverted into colorless LMG in fish (~80%). The special Oxidant Solution in the kit can convert LMG back to the Malachite Green form which can be detected by the kit. 3. Providing a 96-well high throughput quantitative detection method. The detection limit is 0.1 ng/g (ppb) for fish/shrimp samples. 4. The processed samples can be directly used for liquid chromatography confirmation. Since its launch, this kit has generated enormous interest and positive reviews from both government reference labs and private sector labs. See sample reviews on page 13. Also, see our similar product MaxSignal Crystal Violet/ Leucocrystal Violet (LCV) ELISA Test Kit Catalog #: 1029 MaxSignal Histamine Enzymatic Assay Kit (USDA research grant supported*) Catalog #: 1032 The MaxSignal Histamine Enzymatic Assay Kit is a colorimetric enzymatic assay for the determination of histamine in seafood, meat, milk and wine. Unlike ELISA-based methods, this kit uses a rapid and direct enzymatic reaction to determine the amount of histamine in samples. The unique features of the kit are: 1. Very rapid and direct procedure (complete procedure takes 35 minutes from start to finish - no wash steps needed). 2. Convenient aqueous extraction method with high recovery. Harsh extractions using organic solvents not required. 3. High sensitivity and low detection limit (6 ppm). 4. A very rapid and robust enzyme-based assay which does not require chemical derivatization, sample filtration or expensive instrumentation. 5. Direct and simple assay procedure saves time and effort with minimal handling of samples; compatible with scale-up for high-through sample screening. 6. High reproducibility. * USDA SBIR # Food and Feed Safety Testing 5. High reproducibility.

8 B I O O R E S E A R C H & P R E C L I N I C A L T E S T I N G O V E R V I E W M A X I M I Z E S C I E N C E F O R L I F E Bioo Scientific offers a wide range of products to assist with research and preclinical testing. We develop and produce a large assortment of in vivo RNAi products to help facilitate the progress of drug development. We also offer a full line of cytokine ELISAs as well as monoclonal and polyclonal antibodies that cover a wide range of research areas. 6 Research and Preclinical Testing

9 B I O O R E S E A R C H & P R E C L I N I C A L T E S T I N G P R O D U C T S CATEGORY product Name catalog # Quantity* MaxSuppressor TM In Vivo RNA-LANCEr Kit ml MaxSuppressor TM Cell Culture RNA-LANCEr ml ml Delivery Her2 Antibody Conjugated to Protamine mg MaxSuppressor TM Directed Delivery Agents CD33 Antibody Conjugated to Protamine mg VEGFR2 (KDR) Antibody Conjugated to Protamine mg cd4 V1 Domain Antibody Conjugated to Protamine mg MaxSuppressor TM Rnase Free Sterile Saline ml MaxSuppressor TM Rnase Free Sterile Ringers ml Detection MaxSuppressor TM Anti-siRNA Monoclonal Antibody µl MaxSuppressor TM ß-galactosidase ELISA Kit Kit** Reporter Assays MaxSuppressor TM Luciferase ELISA Kit Kit** MaxSuppressor TM D-Luciferin mg MaxSuppressor TM GAPDH ELISA Kit Kit** MaxSuppressor TM Twinfilin 1 ELISA Kit Kit** Controls mir-1 mirna molecule mg MaxSuppressor TM In Vivo Synthetic mirna Controls let-7a mirna molecule mg negative Control mirna molecule mg MaxSuppressor TM Bradford Assay Kit Kit** Other MaxSuppressor TM In Cell ELISA Kit Kit*** *Other quantities are available upon request. **One kit allows for 96 reactions. ***One kit allows for 192 reactions. MaxSuppressor TM Protein Extraction Buffer ml 7 Research and Preclinical Testing

10 Research and Preclinical Testing 8 B I O O C Y T O K I N E A S S A Y S Name cat. # Assay Range (pg/ml) Sensitivity (pg/ml) Mouse GM-CSF Mouse Granzyme B Mouse IFN-y Mouse IL-1α Mouse IL-1ß Mouse IL Mouse IL Mouse IL Mouse IL Mouse IL-12/IL-23 (total p40) Mouse IL-12 p Mouse IL Mouse IL Mouse IL Mouse IL Mouse MCP-1 (CCL2) Mouse TGF-ß Mouse TNF-α Human GM-CSF Human IFN-γ Human IL Human IL-1α Human IL-1ß Human IL Human IL Human IL Human IL Name cat. # Assay Range (pg/ml) Sensitivity (pg/ml) Human IL Human IL Human IL Human IL Human IL Human IL Human IL Human IL Human IL Human IL Human IL Human IL-27p Human IL Human Insulin Human Leptin Human MCP Human TGF-ß Human TGF-ß Human TGF-ß Human TNF-α Human TNF-ß Rat GM-CSF Rat IFN-γ Rat IL-1ß Rat IL Rat IL Rat TNF-α

11 B I O O A N T I B O D I E S Class subclass name/antigen clone Cat. # Quantity* integrin αl (CD11; LFA-1 α subunit) µg Integrins I integrin αl, αm, αx/ß2 (CD11/ CD18) µg Cell Adhesion Proteins icam-1 (CD54) µg Intracellular Adhesion Molecules (ICAMs) icam-3 (CD50) icam µg N neural Adhesion Molecules neural Cell Adhesion Molecule (NCAM; CD56) UJ13A µg T trf1 trf1 3H µg Cell Cycle Proteins trf2 mta18 9F10/ µg cdc2 (Cell Division Control Protein 2; CDK1) µg Cytochrome P450 cyp 1A2 D15 (16VII F10F12) µg DNA Repair & Replication Proliferating Cell Nuclear Antigen (PCNA) PC µg Extracellular Matrix Proteins fibronectin fn µg chitinase Proteins YKL-40 3D µg Growth Factors igf-bp (Insulin-like Growth Factor Binding Protein) igfbp µg Vascular Endothelial Growth Factor (VEGF) Vascular Endothial Growth Factor (VEGF) VG µg cd1c (Human Thymus Leukemia Antigen) m µg C cd3 (Purkinje cell antigen, T3) UCH-T µg C cd4 V1 domain QS µg C cd5 (Leu-1) UCH-T µg C cd5 (Leu-1) H µg C cd8 (Leu-2) µg C cd8 (Leu-2) UCH-T µg C cd8 (Leu-2a) m µg C cd14 UCH-M µg Leukocyte Markers cd35 (Human complement receptor 1; CR1) e µg C cd36 (Thrombospondin receptor) smϕ µg C cd45 (Leukocyte Common Antigen; LCA) c µg C cd45 (Leukocyte Common Antigen; LCA) Hle-1 (2D1) µg C cd45r 351C µg C cd45 RO UCH-L µg C cd55 (Decay Accelerating Factor:DAF) µg C cd64 (Fcg-Receptor I; Fcg-RI) µg C cdw90 (Thy-1) athy-1a µg F fibrin α chain UC µg egfr (HER1) egfr µg Receptors epidermal Growth Factor Receptors (EGFR) c-erb B3 (HER3) sgp µg c-erbb4 (Her4) HFR µg Alkaline Phosphatase (Human Intestinal) AAP µg Signal Transduction Alkaline Phosphatases Placental alkaline phosphatase (PLAP) HD 8B µg bcr-abl 7C µg Signaling Intermediates GAPDH 6C µg Structural Proteins Keratins Keratin 8 LP3K µg Keratin 19 ba µg Tissue Specific Antigens mucin 1 (Muc1) sm µg c-jun (phosphorylated) c-j 4C4/ µg c-myc 9E µg N n-myc nmyc µg Transcriptional Regulators PARN µg RNA Polymerase II (RNAP II) Phosphorylated RNA polymerase II (RNA pol II) CTD 8A µg RNA Polymerase II (RNA pol II) ctd 4H µg Tumor Suppressor Proteins p53 DO µg Rb Rb1 (1F8) µg Human Papilloma Virus (HPV) Proteins HPV-18 E6 (early protein 6) bf µg Viral S Proteins simian Virus 40 (SV40) Proteins sv40 T Antigen PAb µg *Other quantities are available upon request. 9 Research and Preclinical Testing

12 r e s e a r c h & p r e c l i n i c a l f e a t u r e p r o d u c t s Research and Preclinical Testing 10 In Vivo RNA-LANCEr Catalog #: 3407 MaxSuppressor TM In Vivo RNA-LANCEr uses a novel non-toxic blend of fat-soluble naturally-occurring hydrophobic molecules to efficiently deliver RNAi agents into mouse organs. The advanced formulation enables users to deliver RNAi agents, in vivo, for target validation and drug target identification purposes. After formulating RNAi agents with MaxSuppressor TM In Vivo RNA-LANCEr, the RNA becomes stable in serum, preventing the need for stability modifications which reduces the synthesis cost. The preparation time takes less than one (1) hour and can be performed in any standard laboratory setting. Simply mix the RNAi agent with the semi-dry lipid cake of MaxSuppressor TM In Vivo RNA-LANCEr, sonicate and inject as shown in the figure below. Inject Add RNAi agent into (In vivo) RNA-LANCEr Dried lipid cake (In vivo RNA-LANCEr) Sonicate Lipid Bilayer Encapsulated sirna Dried RNAi agent/rna-lancer cake is stable for extended periods of time at -20 C allowing preparation of the small RNAs to be done days before the injection is performed. Injection of this agent via the tail vein has been shown to give the most efficient gene reduction in the liver, lung and kidney. However, the delivery route can be focused so that more efficient uptake and reduction may be obtained in your desired destination. The sirna may be directly injected into a subcutaneous tumor or into a specific organ using surgical procedures. Directed In Vivo Delivery Agents catalog # Her2 Antibody Conjugated to Protamine 3601 CD33 Antibody Conjugated to Protamine 3602 VEGFR2 (KDR) Antibody Conjugated to Protamine 3603 CD4 V1 Domain Antibody Conjugated to Protamine 3604 To assist in the directed delivery of sirna molecules into animals, BIOO offers antibodies conjugated to protamine sulfate. Protamine sulfate is a small, positively charged, arginine rich protein which is traditionally used to reverse the anticoagulant effects of heparin by binding to it. It has been found that protamine sulfate can also associate with nucleic acids such as sirna and mirna molecules. For this reason protamine sulfate conjugated antibodies have significant potential to be used with RNAi agents for the directed delivery of these agents into animal tissues via cell surface receptors. To generate RNAi agent bound to protamine sulfate conjugated antibody, simply mix the RNAi agent with the conjugated antibody in the antibody storage buffer and incubate at 30 C for 15 minutes. Following the incubation the RNA/antibody complex can be diluted into a sterile saline solution and injected directly into the animal. Add RNAi agent into antibodyprotamine conjugate Inject RNAi agent binding to protamine

13 GAPDH ELISA Catalog #: 3401 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis where it serves to break down glucose for energy and carbon molecules. GAPDH has also been found to play a role in membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Particularly useful when studying RNAi induced gene silencing, the MaxSuppressor TM GAPDH ELISA kit can be used to detect GAPDH knockdown in tissues from animals that have been treated with a GAPDH RNAi agent. With this quantitative assay, users can rapidly analyze the impact of sirna or shrna on GAPDH levels in mouse, human or rat organs and body fluids including serum. Human/Mouse Twinfilin 1 ELISA Catalog # This kit enables users to detect twinfilin 1 protein (also known as protein tyrosine kinase 9, A6 protein tyrosine kinase, and PTK9 protein tyrosine kinase 9) in a quantitative fashion in human and mouse derived cells and tissues. This kit contains all of the components needed to extract protein from cells or tissues and to detect twinfilin 1 protein. This ELISA assay is particularly useful for researchers studying mirna function in mammalian cells and animals as mir-1 muscle specific mirna directly impacts twinfilin 1 mrna at the posttranscriptional level. Following the delivery of the mir-1 mirna RNAi agent into cells or organs, the level of twinfilin 1 can be monitored to analyze the impact of delivery both in cell culture and in vivo. This enables users to rapidly analyze delivery of mirna RNAi agents using an easy and cost effective ELISA assay. This method is more economical than performing PCR in standard conditions and allows for a more physiological impact of mirna function a s compared with using PCR. ß-galactosidase ELISA Catalog # ß-galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of ß-galactosides into monosaccharides. In E. coli the ß-galactosidase protein is encoded by the lacz gene which is part of the lac operon. The ß-galactosidase protein is a large (120 kda, >1000 amino acids) protein that forms a tetramer. The enzyme s function in E. coli is to cleave the disaccharide lactose to form glucose and galactose so they can be used as carbon/energy sources. The lacz gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of gene promoters. ß-galactosidase is not typically expressed in mammalian cells, however; ß-galactosidase positive staining is a marker for mammalian cellular senescence. ß-galactosidase is a useful reporter gene both in cell culture and in vivo applications due to its ease of detection. This ELISA kit contains all the reagents required to quantitate the level of ß-galactosidase protein in cells and tissues. This kit can also be used to study the influence of drug treatments on ß-galactosidase staining in animal organs or cells in culture. Luciferase ELISA Catalog # Luciferase is a generic name for enzymes, commonly found in nature, that produce light. The most commonly used Luciferase is produced by, Photinus pyralis, the firefly. Luciferase catalyzes a chemiluminescent oxidation reduction reaction and although many kits are available to detect the enzymatic activity of this protein using light emission, these methods have several drawbacks. These assays have rapid decay kinetics; require the enzymatic activity of the Luciferase to be retained and they do not enable absolute quantification. In response to these issues, BIOO has developed a highly sensitive ELISA assay which allows the researcher to completely measure the amount of firefly Luciferase in cell or tissue lysates. This ELISA Kit provides all of the components needed for rapid, and precise, quantification of firefly, Photinus pyralis, Luciferase in cultured mammalian cells or tissues. 11 Research and Preclinical Testing

14 BIOO services M A X I M I Z E S C I E N C E F O R L I F E Research and Preclinical Testing 12 Bioo Scientific offers services in three main areas: 1. Preclinical Testing Services: Our Preclinical Testing Services provide a cost effective way of testing specific drug molecules, including RNA based drugs, in the small animal model system most appropriate for your specific goals. This service enables the researcher to take potential drugs against clinically important targets into a preclinical setting for further validation in an efficient fashion. 2. In vivo RNAi Agent Services: BIOO offers custom RNAi services in the area of the whole organism. These experiments are typically not easy for most researchers to do as the techniques are very specialized and time consuming to initiate. We offer a cost effective way of testing specific sirna molecules in the model system most appropriate for your goals. This enables researchers the ability to take sirnas against clinically important targets directly into an animal environment for further validation in an efficient fashion with minimal down time. 3. Antibody Services: We offer both monoclonal and polyclonal antibody production services. BIOO has developed a unique 5-week Monoclonal Antibody Production Service (patent-pending) to produce custom monoclonal antibodies against peptides and proteins. We understand the importance of antibody availability for research needs and therapeutic development. The benefit of BIOO s high volume throughput production allows end-users to access a greater number of antibody hybridomas for significantly less cost. We provide full antibody services for our customers, from antigen design to affinity purification and ascite fluid production. Using our exclusive method we can generate an effective antibody where other systems fail. We have state of the art facilities where we perform these services and research expertise that is aggressively advancing the technology of in vivo RNAi activity, efficacy and delivery and monoclonal antibody manufacturing. Our continued efforts in these areas will keep our service offering on the cutting edge of science. BIOO Services provide customers quality results with minimal start up and overhead costs. Bioo Scientific has a >2000 square foot, USDA and OLAW approved, Animal Facility. This facility is fully functioning to test drug compounds and RNAi agents in numerous animal model systems. We follow all guidelines as specified by OLAW and PHS Policy and take tremendous care to provide a comfortable and safe environment for all animals. BIOO Services provide a means of outsourcing preclinical testing, in vivo RNAi agent experiments and monoclonal and polyclonal antibody-manufacturing to highly trained professionals. We can help researchers achieve their objectives at a fraction of the cost and with minimal down time.

15 c u s t o m e r r e v i e w s & c o n t a c t i n f o r m a t i o n Re: Bioo Scientific Corporation s MaxSignal TM Malachite Green Kits The recent FDA recall of potentially contaminated seafood products created quite a concern among several of our valued clients. Our job at Q Laboratories was to alleviate these concerns by providing rapid and accurate malachite green results. The Bioo Scientific MaxSignal TM kits allowed us to meet the needs of our clients and the subsequent governing bodies in a timely manner. The new changes in the test kit standards are even further proof that Bioo Scientific works hard to improve the quality of their products and we will continue to turn to them for our test kit needs in the future. Re: Bioo Scientific Corporation s MaxSignal TM Malachite Green Kits Gorton s relies on Bioo Scientific s MaxSignal TM Malachite Green Kits to provide our laboratory with a rapid, sensitive and cost effective procedure to screen our products. The availability of the kit allowed Gorton s to react quickly to current food safety and regulatory issues surrounding food contaminants. We are pleased with the accuracy and continuous improvements to the procedure developed by Bioo Scientific, which has resulted in a greater ease of use by laboratory technicians. Erin S. Crowley Microbiology R&D Supervisor Q Laboratories, Inc. Cincinnati, Ohio Allison Williamson QA Manager Gorton s 13 contact Tel: Fax: info@biooscientific.com M A X I M I Z E S C I E N C E F O R L I F E Sales & Customer Support US: Outside US: Contact your local distributors or distribution@biooscientific.com sales@biooscientific.com support@biooscientific.com antibodyservice@biooscientific.com foodfeedsafety@biooscientific.com RNAi@biooscientific.com For up-to-date information on Bioo Scientific Corp s complete range of products and services, go to Research and Preclinical Testing

16 Bioo Bioo Scientific Scientific Corp. Corp Todd Todd Lane, Lane, Ste. Ste Austin, TX USA USA e: e: Made 2008 in USA Bioo Scientific Corp. M A X I M I Z E S C I E N C E F O R L I F E