Focus on Preventing Disease. keeping an eye on a healthy bottom line. Cattle Industry

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1 Focus on Preventing Disease keeping an eye on a healthy bottom line Cattle Industry

2 Multimin + VACCINES : University OF FLORIDA study data Study 1 Effect of injectable trace minerals on the humoral immune response to multivalent vaccine administration in beef calves J.D. Arthington and L.J. Havenga Brief Protocol: 99 beef steer calves average bodyweight = 695 ± 9.24lbs. Serum concentrations of copper, zinc, manganese and selenium indicated there were no pre-existing trace mineral deficiencies among the steers. Seronegative for bovine herpes-1 (BHV-1) and bovine viral diarrhea virus, genotypes 1 and 2 (BVDV-1 and BVDV-2). Calves received a single dose of multivalent modified live vaccine containing BHV-1, BVDV-1 and BVDV-2, bovine parainfluenza virus type 3 and bovine respiratory syncytial virus. 15 non-vaccinated sentinel calves in group. Among the vaccinated calves, 2 treatments were concurrently and randomly applied: 1) Multimin 2) Saline Study Results The study results indicated that Multimin administered concurrently to multivalent viral vaccine contributes to the immune response! The production of neutralizing antibody titers against BHV-1 was increased statistically significantly in beef steers as early as 14 days after vaccination and this increase was maintained for at least 60 days after vaccination and Multimin injection. Bovine herpesvirus-1 (BHV-1) serum titers (log 10) of calves provided a 7-mL injection of trace minerals (ITM) or 7 ml of sterile saline (Control). Seronegative calves vaccinated on d 0. Treatment day; P < * = Values within day and between treatments differ; P < The bottom line There is no reason not to use MULTIMIN with a modified live viral vaccine.

3 Because PREVENTING DISEASE = PROFIT! University OF FLORIDA study data Study 2 Effects of injectable trace minerals on the humoral immune response to porcine red blood cell challenge and fertility in beef heifers P. Moriel 1, P.G.M.A. Martins *1, G.C. Lamb 2, L. J. Havenga 3, and J.D. Arthington 1 1 University of Florida, Range Cattle Research and Education Center, Ona. 2 University of Florida, North Florida Research and Education Center, Marianna. 3 Multimin USA, Inc., Fort Collins, CO. Brief Protocol: 33 yearling heifers were randomly assigned to receive 3 ml per heifer Multimin or sterile saline (Control) on the same day as a 25% porcine red blood cell solution. The heifers grazed winter, stockpiled limpograss pastures and were provided free-choice, stock salt with no added trace minerals. Heifers were challenged with a 10 ml injection of a 25% porcine red blood cell solution as a novel pathogen exposure. Study Results Average daily gain was greater (P=0.06) for Multimin treated heifers. (0.69 vs 0.57 lb/d). Heifers treated with Multimin had more PRBC neutralizing antibody titers on day 3, 7, and 14 following challenge compared to control heifers. Liver concentrations of Se were greater (P < 0.01) in Multimin versus control heifers. Porcine red blood cell (PRBC) neutralizing antibody titers measured on d 0, 3, 7, 14, and 21 relative to PRBC injection. P * 0.10 and ** The bottom line MULTIMIN may increase bodyweight gain, humoral immune response and trace mineral status of growing heifers.

4 INNATE IMMUNITY IMMEDIATE IMMUNE RESPONSE First line of defense Broad action No immunological memory Provides the time required by the acquired immune system to develop an antibody response Protection on the outside: Skin Protection on the inside: Mucous membranes Mucous blanket Ciliated epithelial cells Fighting the 1st stage of infection: Phagocytes (white blood cells - e.g., macrophages & neutrophils) Locate and kill pathogens before they proliferate and cause significant infection Immunity ACquired IMMUNITY Longer term IMMUNE RESPONSE Takes time to develop Antibodies *Antigen-specific (*substances foreign to the body such as bacteria, viruses, and vaccines - induce the immune system to respond) Immunological memory Defense cells in the blood: B lymphocytes (white blood cell - produces antibodies) Antibodies/immunoglobulins (neutralize antigen / mark antigens ) Antigen-specific memory memory cells Circulate in blood humoral immunity Defense cells in the body tissue: T lymphocytes (white blood cell) Antigen-specific Destroy pathogens & infected cells cell mediated immunity Acute Phase Reaction tissue repair and remodeling Neutrophils Migration & survival Zinc maintains the thymus gland, which produces mature T-cells in cellular immunity. Production & function glutathion peroxidase enzyme Copper in Cu-SOD 1 Zinc in Zn-SOD 1 Stabilizes cell membranes and protects cells Manganese in Mn-SOD 1 1 SOD = Superoxide Dismutase enzyme to drive the immune response: Energy production Protein synthesis & cell replication Maintains feed intake: Increases nutrients for immunity Decreases cortisol due to nutritional stress (which inhibits immunity) T 4 -> T 3 T 4 -> T 3

5 combining MULTIMIN with a modified live vaccine Did you know that modified live vaccines impact the trace mineral status of recently vaccinated animals? This is due to different trace minerals playing an active role in different pathways of the acquired immune response. This may be critical for optimal vaccine response and disease prevention in cattle. USE ONLY IN CATTLE KEEP OUT OF REACH OF CHILDREN (AN INJECTABLE CHELATED SUPPLEMENTAL SOURCE OF ZINC, MANGANESE, SELENIUM AND COPPER) CAUTION: Federal Law restricts this drug to use by or on the order of a licensed veterinarian. GUARANTEED ANALYSIS: Zinc mg/ml Manganese mg/ml Selenium... 5 mg/ml Copper mg/ml copper selenium CAUTION: Slight local reaction may occur for about 30 seconds after injection. A slight swelling may be observed at injection site for a few days after administration. Use standard aseptic procedures during administration of injections. Store Between 15 C and 30 C (59 F and 86 F). Protect from light. INGREDIENTS: Zinc oxide, manganese carbonate, copper carbonate, sodium selenite, disodium EDTA, sodium hydroxide, chlorocresol 0.1% (as preservative). DIRECTIONS: USE ONLY IN CATTLE BY SUBCU- TANEOUS OR INTRAMUSCULAR INJECTION. Zinc ManGanese Serum mineral concentrations at d 0 and 14 relative to vaccination and treatment administration. Samples analyzed from a random sub-sample of steers in each treatment (n = 15 steers/treatment). Steers provided a 7-mL injection of trace minerals (ITM) or 7 ml of sterile saline (Control) on d 0. Treatment time, P 0.05, 0.33, 0.01, and for Zn, Mn, Cu, and Se, respectively. Values with unlike letters differ (P < 0.05) between treatments within sampling days (a,b) and within treatments across sampling days (x,y). Study data indicated that MULTIMIN treatment at the same time as vaccination maintains/improves the animal s trace mineral status. This may be critical in driving the immune response for better health and also for breeding cattle. For the complete trial data, please visit our website DOSAGE RECOMMENDATIONS: CALVES: up to 1 year... 1 ml/per 100 lbs. bodyweight CALVES: From 1-2 years... 1 ml/per 150 lbs. bodyweight CALVES: Over 2 years... 1 ml/per 200 lbs. bodyweight SUPPLEMENTATION PROGRAM: Bulls... 3 times per year Beef Cows... 4 weeks before breeding 4 weeks before calving Dairy Cows... 4 weeks before calving 4 weeks before insemination 4 weeks before dry-off Calves... at birth at 3 months and/or weaning Heifers... every 3 months especially 4 weeks before breeding Additional... every 2 months in wet conditions (Program gives planned dates that can be varied to suit management programs) Packaged in 100 ml NDC No & 500 ml size US PATENT # 7,285,292 NDC No Copyright 2012, MultiMin USA, Inc. All Rights Reserved. B Get the Facts

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