DNA SEQUENCING 1997/8 EUROPEAN MSPPSA SERIES

Transcription

1 1997/8 EUROPEAN MSPPSA SERIES DNA SEQUENCING AN ANALYSIS OF MARKET SIZE & GROWTH MARKET SHARE PURCHASE PLANS & SUPPLIER ASSESSMENT FOR THE EUROPEAN LIFE SCIENCE RESEARCH MARKET A Multi-Client Report by PhorTech International San Carlos, California April 27, 1998 Copyright 1998 by PhorTech International, 238 Crestview Drive, San Carlos CA All rights reserved. No material contained in this report may be reproduced in whole or in part without the written permission of the publisher. This report is not intended to be, and should not be construed as a recommendation for the purchase or sale of any securities mentioned herein. The information has been derived from statistical and other sources which we deem reliable but their completeness cannot be guaranteed. Opinions expressed herein are based upon our interpretation of available information and are subject to change.

2 1997/8 European MSPPSA DNA Sequencing -2-

3 TABLE OF CONTENTS I. BACKGROUND... 9 A. Survey Objectives B. Survey Methodology II. DEMOGRAPHIC SEGMENTATION #0. Geographic Segmentation of Respondents #40. Segmentation by Organizational Type #10. Sequencing Service Utilization #41. Providers of Sequencing Services #42. Respondents Working in Core Facilities III. MARKET SIZE & GROWTH #0+. European Market Size for Life Scientists #1. Current Use of DNA Sequencing, Market Size #2. Sharing of Instrumentation: Reported Group Sizes #3. Manual Sequencing Unit Installed Base Audit #12. Advanced Sequencing Instruments Installed Base Audit. 54 #A. Estimate of European Molecular Biology Rgt Market IV. MARKET SHARE #3+. Manual Sequencing Unit & Dollar Market Shares #12+. Advanced Instrumentation Unit & Dollar Market Shares 89 #27+ Thermal Cycler Unit & Dollar Market Shares V. PURCHASE PLANS #6. Forecast Change in Manual Sequencing #19. Forecast Change in Automated Sequencing #28. Future Purchase Plans for All Instrumentation #29. Instruments & Suppliers Considered for Purchase VI. CURRENT SEQUENCING METHODOLOGY #9. Manual Workload - Templates/Week & Bases Called #18. Automated Workload - Templates/Week & Bases Called 140 #11. Access to Advanced Instrumentation #20. Primary Applications for Automated Sequencing #21. Throughput for Non-Sequencing Applications #22. Primary Labeling Methods for Automated Sequencing #30. Sequencing Kit Usage vs. Individual Reagents #31. Suppliers of Sequencing Kits & Reagents #32. Current Sequencing Strategies in Use #33. Distribution of Template Types #34. Single-Stranded Template Purification Methods #35. Plasmid Template Purification Methods #36. PCR-Generated Seq. Template Purification Methods

4 1997/8 European MSPPSA DNA Sequencing #37. Methods for Sequencing Templates #38. Average Consumption by Individual Researchers #39. Current Size of Sequencing Project VII. SUPPLIER ASSESSMENT #4. Reasons for Choosing Last Manual Seq. Purchased #5. Customer Satisfaction: Manual Sequencers #5+. Rejected Manual Sequencer Brands & Reasons #8. Ranked Supplier Performance - Manual Sequencers #13. Reasons for Choosing Last Automated Seq. Purchased #14. Customer Satisfaction: Automated Sequencers #14+. Reasons Behind Satisfaction: Automated Sequencers #17. Ranked Supplier Performance: Automated Sequencers #23. Reasons for Choosing Last Robotic Workstation #24. Customer Satisfaction: Robotic Workstations #26. Ranked Supplier Performance: Robotic Workstations VIII. FUTURE EXPECTATIONS #7. Desired Improvements in Manual Sequencers #15. Most Important Features of Automated Sequencers #16. Desired Improvements in Automated Sequencers #25. Desired Improvements in Robotic Workstations IX. QUESTIONNAIRE

5 LIST OF TABLES & FIGURES I. BACKGROUND... 9 Survey Objectives Survey Methodology Survey Response Rates II. DEMOGRAPHIC SEGMENTATION Geographic Segmentation for Seven Sources of Names Respondent s Geographic Distribution by European Regions Distribution by Type of Organization, DNA Sequencing Users Distribution by Type of Organization, Automated DNA Sequencing Users Distribution by Type of Organization, Manual DNA Sequencing Users Utilization of Sequencing Service, All Respondents Laboratory Provides Sequencing Service, DNA Sequencing Users Laboratory Provides Sequencing Service, All Automated Sequencing Users Use vs. Provide Sequencing Service, All Respondents Respondents Working in Core Facilities, All Respondents Respondents Working in Core Facilities, Automated DNA Sequencing Users III. MARKET SIZE & GROWTH European Life Scientist Population Estimates by Overlap Analysis DNA Sequencing Usage by Source Eur Population Estimate of DNA Sequencer Users DNA Sequencing Usage Rate, by Region, Respondents from Unbiased Sources European DNA Sequencing Users Population Estimate, by Geographic Region European Usage of Manual & Automated Sequencers, All Respondents Usage of Various Instrumentation, All User-Respondents Reported Group Size, Respondents Performing Sequencing Reported Group Size, According to Type of Instrument Reported Average Group Size for Users, According to Type of Instrument Mean & Median Number of Manual Sequencers per User, Installed Base Total Manual Sequencing Instruments per Respondent/Group Average Number of Manual Sequencers per Individual Researcher Mean & Median Number of Manual Sequencers per Respondent by Region Expenditure on Manual Sequencers per Respondent/Group, Installed Base Mean & Median Expenditure on Manual Sequencers per Respondent by Region. 49 Expenditure on Manual Sequencers per Individual Researcher, Installed Base Extrapolation of Installed Base of Manual Sequencers, by Geographic Region Unit Placements by Year, Manual DNA Sequencing Unit Installed Base Dollar Placements by Year, Manual DNA Sequencing Unit Installed Base Annual European Manual DNA Sequencer Sales Estimate, by Year Advanced Sequencing Instrumentation, Unit Share by Category Advanced Sequencing Instrumentation, Dollar Share by Category Total Automated Sequencing Instruments per Respondent s Group

6 1997/8 European MSPPSA DNA Sequencing Mean & Median Values for Advanced Instruments per Resp, Installed Base Median & Mean Number of Automated Sequencers, by Geographic Region Total Automated Sequencing Instruments, per Individual Researcher Expenditure on Automated Sequencers, per Respondent s Group Expenditure on Automated Sequencers, per Individual Researcher Average Expenditure on Automated Sequencers, by Geographic Region Extrapolation of Installed Base of Automated Sequencers, by Geographic Region. 59 Units Placements by Year, Automated Sequencers Installed Base Dollar Placements by Year, Automated Sequencers Installed Base Annual European Automated DNA Sequencer Sales Estimate, by Year Calculated CAGR for Automated Sequencers, 1994 to present Number of Other Advanced Instruments per Respondent s Group Number of Other Advanced Instruments per Individual Researcher Average Quantity of Robotic Workstation & Software Used for Sequencing Expenditure on Robotic Workstations, per Respondent s Group Expenditure on Robotic Workstations, per Individual Researcher Expenditure on Sequencing Analysis Software, per Respondent s Group Expenditure on Sequencing Analysis Software, per Individual Researcher Average Expenditure on Robotic Wkstns & Software, by Region Extrapolation of Installed Base of Robotic Workstations, by Geographic Region.. 65 Extrapolation of Installed Base of Analysis Software, by Geographic Region Units Placements by Year, Robotic Workstation Installed Base Dollar Placements by Year, Robotic Workstation Installed Base Annual European Robotic Workstation Sales Estimate, by Year Units Placements by Year, Sequence Analysis Software Installed Base Dollar Placements by Year, Sequence Analysis Software Installed Base Annual European Sequencing Analysis Software Sales Estimate, by Year Average Number of Thermal Cyclers Reported per Respondent s Group Average Number of Thermal Cyclers Reported per Individual Researcher Median & Mean Number of Thermal Cyclers, by Geographic Region Expenditure on Thermal Cyclers, per Respondent/Group Expenditure on Thermal Cyclers, per Individual Researcher Average Expenditure on Thermal Cyclers, by Geographic Region Extrapolation of Installed Base of Thermal Cyclers, by Geographic Region Units Placements by Year, Thermal Cycler Installed Base Dollar Placements by Year, Thermal Cycler Installed Base Annual European Thermal Cycler (for Sequencing) Sales Estimate, by Year European Sales Estimate for All Sequencing Instrumentation, by Year Dollar Market Worth by Geographic Region, Automated Sequencing Units IV. MARKET SHARE Manufacturers Unit Market Shares for Manual DNA Sequencers Manufacturers Dollar Market Shares for Manual DNA Sequencers Average Manual Sequencer Prices for Major Suppliers Most Frequently Mentioned Manual DNA Sequencer Models

7 Manual DNA Sequencer Unit Market Share, Sequencers Sold Since Manual DNA Sequencer Dollar Market Share, Sequencers Sold Since Manufacturers $ Market Shares for Manual Sequencers, English-speaking Rgn Manufacturers $ Market Shares for Manual Sequencers, German-speaking Rgn.. 86 Manufacturers $ Market Shares for Manual Sequencers, Scandinavian Rgn Manufacturers $ Market Shares for Manual Sequencers, France & Southern Eur 87 Manufacturers $ Market Shares for Manual Sequencers, Benelux Manufacturers Unit Market Shares, Automated Sequencer Installed Base Manufacturers Dollar Market Shares, Automated Sequencer Installed Base Most Frequently Mentioned Automated Sequencer Models Manufacturers Dollar Market Shares, Automated Sequencers Sold Since Manufacturers $ Market Shares for Auto Sequencers, English-speaking Rgn Manufacturers $ Market Shares for Auto Sequencers, German-speaking Rgn Manufacturers $ Market Shares for Auto Sequencers, Scandinavian Rgn Manufacturers $ Market Shares for Auto Sequencers, France & Southern Eur Manufacturers $ Market Shares for Auto Sequencers, Benelux Manufacturers Unit Market Shares, Robotic Workstation Installed Base Manufacturers Dollar Market Shares, Robotic Workstation Installed Base Average Robotic Workstation Prices for Major Suppliers Manufacturers Unit Market Shares, Sequence Analysis Software Installed Base Manufacturers Dollar Market Share, Sequence Analysis Software Installed Base.. 97 Average Sequence Analysis Software Prices for Major Suppliers Most Frequently Mentioned Sequencing Analysis Software Models Manufacturers Unit Market Shares, Thermal Cycler Installed Base Manufacturers Dollar Market Share, Thermal Cycler Installed Base Average Prices for Major Suppliers of Thermal Cyclers Thermal Cycler Sample Capacity, Share of Installed Base Average Price Paid for Various Thermal Cycler Sample Capacities Thermal Cycler Sample Capacity, Dollar Market Share Most Frequently Mentioned Thermal Cycler Models Manufacturers Dollar Market Share, Thermal Cyclers Sold Since Manufacturers $ Market Shares for Thermal Cyclers, English-speaking Rgn Manufacturers $ Market Shares for Thermal Cyclers, German-speaking Rgn Manufacturers $ Market Shares for Manual Sequencers, Southern Europe Manufacturers $ Market Shares for Manual Sequencers, France Manufacturers $ Market Shares for Thermal Cyclers, Scandinavian Rgn Manufacturers $ Market Shares for Manual Sequencers, Benelux V. PURCHASE PLANS Forecast Change in Manual Sequencing, All Manual Sequencing Users Aver. Change in Manual Sequencing Over Next 12 Months by Current Users Verbatim Comments Reasons Behind Anticipated Change in Manual Sequencing 114 Verbatim Comments From Previous Manual Sequencing Users Forecast Change in Automated Sequencing, Automated Sequencing Users Average Change in Auto Sequencing Over Next 12 Months by Current Users Verbatim Comments Regarding Forecast Change in Automated Sequencing Use

8 1997/8 European MSPPSA DNA Sequencing Purchase Plans for Auto Sequencers, Response Profiles of Users & Non-Users /98 European Sales Projections for Automated Sequencers Purchase Plans for Robotic Workstations, Response Profile of Current Users /98 European Sales Projections for Robotic Workstations Purchase Plans for Analysis Software, Response Profile of Current Users /98 European Sales Projections for Analysis Software Purchase Plans for Thermal Cyclers, Response Profile of Current Users /98 Eur Sales Projections for Thermal Cyclers Used for Cycle Sequencing Share of Mentions, Planned Purchases of Automated DNA Sequencers Share of Mentions, Planned Purchases of Robotic Workstations Share of Mentions, Planned Purchases of Sequencing Analysis Software Share of Mentions, Planned Purchases of Thermal Cyclers VI. CURRENT SEQUENCING METHODOLOGY Average Templates Sequenced per Week, Cross Section of Manual Seq. Users Mean, Median & Mode: Templates Sequenced/Week by Manual Sequencing Mean, Median & Mode: Average Gels Run per Week by Manual Sequencing Average Gels Run per Week, Cross Section of Manual Seq. Users Mean, Median & Mode: Average Bases Called/Template by Manual Sequencing. 136 Average Bases Called per Template, Cross Section of Manual Sequencing Users Average Bases Sequenced per Week, Cross Section of Manual Sequencing Users. 137 Mean, Median & Mode: Average Bases Sequenced/Week by Manual Sequencing 137 Average Reagent Cost per Template, Cross Section of Manual Seq. Users Mean, Median & Mode: Reagent Cost/Template Using Manual Sequencing Mean, Median & Mode: Average Reagent Cost/Week Using Manual Sequencing 138 Average Reagent Cost per Week, Cross Section of Manual Sequencing Users Summary of Mean & Median Values for Manual Sequencing Users Average Templates Sequenced per Week, Cross Section of Automated Seq Users 140 Mean, Median & Mode: Average Templates Sequenced/Week by Auto Seq Mean, Median & Mode: Bases Called per Template by Automated Sequencing Average Bases per Template, Cross Section of Automated Seq. Users Average Bases Sequenced per Week Called, Cross Sectn of Automated Seq. Users 142 Mean & Median: Average Reagent Cost/Template by Automated Sequencing Average Reagent Cost per Template, Cross Section of Automated Seq. Users Average Reagent Cost per Week, Cross Section of Automated Sequencing Users. 143 Summary of Mean & Median Values for Automated Sequencing Users Access to Sequencing Instrumentation, Current Users Verbatim Description of Applications for Automated Sequencing Percentage Throughput for Non-Sequencing Applications Non-Sequencing Applications, Percentage Throughput on Auto Sequencers Verbatim Description of Non-Sequencing Applications & Level of Throughput Non-Sequencing Applications, Share of Mentions Primary Labeling Methods Utilized by Automated Sequencing Users Sequencing Labeling Methods, Dye Primer vs Dideoxy Dye Terminator Verbatim Description of Other Labeling Methods for Automated Sequencing Reported Percentage of Kit Use, All Sequencing Users

9 Reported Percentage of Reagent Use, All Sequencing Users Preferred Brands of Sequencing Kits, Share of Mentions Preferred Brands of Sequencing Enzymes, Share of Mentions Preferred Brands of Nucleotides, Share of Mentions Preferred Brands of Sequencing Buffers, Share of Mentions Preferred Brands of Gel Matrix Reagents, Share of Mentions Preferred Brands of Other Reagents, Share of Mentions Current Sequencing Strategies in Use Verbatim Description Regarding Other Sequencing Strategies Used Current Sequencing Strategies in Use, Manual vs. Automated Sequencing Users Template Preparation Techniques, Share of Mentions Percentage of Total Templates, PCR Generated Percentage of Total Templates, Plasmid Vector Percentage of Total Templates, M13/Phagemid Vector Percentage of Total Templates, Lambda/Cosmid Vector Percentage of Total Templates, Other Template Types Write-In Responses for Sequencing Templates Average Number of Templates, All Users vs. Automated Sequencing Users Verbatim Description Regarding Single Stranded Template Purification Method. 176 Single Stranded Template Purification Methods, Reported Brand Names Plasmid Template Purification, All Sequencing Users Verbatim Description of Other Methods for Plasmid Template Purification Plasmid Template Purification, Manual vs. Automated Sequencing Users PCR-Generated Template Purification, All Sequencing Users Verbatim Descriptn of Other Methods for PCR-Generated Template Purificat n. 183 PCR-Generated Template Purification, Manual vs. Automated Sequencing Users 184 Current Methods for Sequencing Templates, All Users Current Methods for Sequencing Templates, Manual vs. Automated Sequencing 186 Sequencing Enzyme Use, Share of Mentions Templates Sequenced per Week, Sequenase Templates Sequenced per Week, AmpliTaq FS Templates Sequenced per Week, Thermosequenase Templates Sequenced per Week, Taq Polymerase Templates Sequencing per Week, AmpliTaq CS Templates Sequenced per Week, Sequitherm Templates Sequenced per Week, Reverse Transcriptase Templates Sequenced per Week, Bst Polymerase Templates Sequenced per Week, Other Enzymes Median Number of Templates Sequenced Weekly, Various Sequencing Enzymes 194 Share of All Templates Used Weekly for Each Sequencing Enzyme Sequencing Enzyme Use, Manual vs. Automated Sequencing Users Monthly Sequencing Output, All Sequencing Users Monthly Sequencing Output, Automated Sequencing Users VII. SUPPLIER ASSESSMENT Last Manual Sequencer Purchased

10 1997/8 European MSPPSA DNA Sequencing Verbatim Comments for Last Purchased Manual Sequencer Sorted by Brand Satisfaction with Last Purchased Manual Sequencer Customer Satisfaction By Last Purchased Brand of Manual Sequencer Satisfaction Rates & Confidence Levels for Manual Sequencer Manufacturers Reasons for Dissatisfaction with Last Manual Seq. Purchase Sorted by Brand Reasons for Satisfaction with Last Manual Sequencer Purchase Sorted by Brand Ranked Manufacturers Performance for Manual Seq.: Best Value for Money Ranked Manufacturers Performance for Manual Seq.: Easiest to Use Ranked Manufacturers Performance for Manual Seq.: Most Reliable Quality Ranked Manufacturers Performance for Manual Seq.: Best Tech Support Last Automated Sequencer Purchased, All Users Verbatim Comments for Last Purchased Automated Sequencer Sorted by Brand Satisfaction with Last Purchased Automated Sequencer, Share of Mentions Satisfaction Ratings By Last Purchased Brand of Automated Sequencer Satisfaction Rates & Confidence Levels for Automated Sequencer Manufacturers. 224 Reasons for Dissatisfaction with Last Automated Seq. Purchase Sorted by Brand Reasons for Satisfaction with Last Automated Seq. Purchase Sorted by Brand Ranked Manufacturers Performance for Automated: Best Field Service Ranked Manufacturers Performance for Automated: Easiest to Use Ranked Manufacturers Performance for Automated: Greatest Innovator Ranked Manufacturers Performance for Automated: Highest Throughput Ranked Manufacturers Performance for Automated: Lowest Running Costs Ranked Manufacturers Performance for Automated: Most Reliable Quality Ranked Manufacturers Performance for Automated: Most Committed Last Robotic Workstation Purchased, All Users Reason for Selecting Brand of Robotic Workstation Sorted by Brand Satisfaction with Last Purchased Robotic Workstation Verbatim Comments Regarding Dissatisfaction with Robotic Wkstn, by Brand Verbatim Comments Regarding Satisfaction with Robotic Wkstn, by Brand Ranked Manufacturers Performance for Robotics: Application Flexibility Ranked Manufacturers Performance for Robotics: Best Field Service Ranked Manufacturers Performance for Robotics: Easiest to Use Ranked Manufacturers Performance for Robotics: Level of Automation Ranked Manufacturers Performance for Robotics: Most Reliable Quality VIII. FUTURE EXPECTATIONS Suggested Improvements in Manual DNA Sequencers Most Frequently Mentioned Areas for Improvement, Manual Sequencers Most Important Features of Automated Sequencers Most Frequently Mentioned Features, Automated Sequencers Suggested Improvements in Automated DNA Sequencers Suggested Improvements in Automation Capabilities IX. QUESTIONNAIRE