PCR Instruments and Consumables

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "PCR Instruments and Consumables"

Transcription

1 Index Page GeneAmp PCR Instrument Systems 2 GeneAmp PCR System 9700 Components 3 Accessories and Software for GeneAmp PCR Instrument Systems 4 Disposables 5 PCR Enzymes 7 GeneAmp PCR Kits 12 RNA PCR Kits 15 Other Reagents and Disposables 18 Training 20 Ordering Information 20 Trademarks 23 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 1 of 23

2 GeneAmp PCR Instrument Systems Gold 96-Well GeneAmp PCR System 9700 Each 9.900,00 High performance, high speed 96-well PCR system with gold-plated silver block and graphical user interface for easy, intuitive programming. Interchangeable sample block. Includes user s manual on CD and disposables starter pack Aluminum 96-Well GeneAmp PCR System 9700 Each 7.950,00 High performance 96-well PCR system with aluminium block and graphical user interface for easy, intuitive programming. Interchangeable sample block. Includes user s manual on CD and disposables starter pack Dual 96-Well GeneAmp PCR System 9700 Each ,00 High performance, high speed 2 x 96-well PCR system with aluminium block and plate ejector and graphical user interface for easy, intuitive programming. Interchangeable sample block. Includes user s manual on CD. N Dual 384-Well GeneAmp PCR System 9700 Each ,00 High performance, high throughput 2x 384-well PCR system and graphical user interface for easy, intuitive programming. Interchangeable sample block. Includes user s manual on CD and disposables starter pack Auto-Lid Dual 384-Well GeneAmp PCR System 9700 Each ,00 High performance, high throughput 2x 384-well PCR system with automated heated lid to enable robotic sample loading and unloading. Interchangeable sample block. Includes user s manual on CD and disposables starter pack Well 0.5 ml GeneAmp PCR System 9700 Each 8.400,00 High performance 60-well PCR system for oil free PCR in 0.5 ml tubes. Graphical user interface for easy, intuitive programming and interchangeable sample block. Includes user s manual on CD and disposables. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 2 of 23

3 GeneAmp PCR System 2700 Each 4.800,00 Personal-sized high performance PCR System with fixed 96-well sample block and graphical user interface for easy, intuitive programming. Includes user s manual on CD and disposables starter pack. GeneAmp PCR System 9700 Components N GeneAmp PCR System 9700 Base Module Each 7.900,00 Includes user s manual on CD. GeneAmp PCR System 9700 Base Module User s Manual, P/N , can also be downloaded from the internet as PDF file at: GeneAmp PCR System Well Gold Sample Block Each 3.540,00 Module Includes user s manual on CD. GeneAmp PCR System Well Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: GeneAmp PCR System Well Aluminium Sample Each 1.840,00 Block Module Includes user s manual on CD. GeneAmp PCR System Well Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: GeneAmp PCR System 9700 Dual 96-Well Sample Block Each 8.000,00 Module Includes user s manual on CD. User s Manual can also be downloaded from the internet as PDF file at: N GeneAmp PCR System 9700 Dual 384-Well Sample Each 8.000,00 Block Module Includes user s manual on CD. GeneAmp PCR System 9700 Dual 384-well Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 3 of 23

4 GeneAmp PCR System 9700 Auto Lid Dual 384-Well Each ,00 Sample Block Module Includes user s manual on CD. GeneAmp PCR System 9700 Dual 384-well Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: GeneAmp PCR System Well Sample Block Each 2.400,00 Module Includes user s manual on CD. GeneAmp PCR System ml Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: C GeneAmp Thermal Isolation Frame Each 76,00 Accessories and Software for GeneAmp PCR Instrument Systems ml Temperature Verification Kit Each 2.740,00 For use with the 96-Well GeneAmp PCR System 9700, the GeneAmp PCR System 9600 and the GeneAmp PCR System ml Temperature Verification Kit Each 2.740,00 For use with the 60-Well GeneAmp PCR System 9700 and the DNA Thermal Cycler ml Temperature Verification Kit Each 2.740,00 For use with the Dual 384-Well GeneAmp PCR System Method Storage PCMCIA Card for 9700, 260K. Each 178,00 N Mac Communication Cable Each 7, Networking Software Kit for GeneAmp PCR System Each 1.970, Includes: Software Application CD, PCMCIA Card with Firmware, 1x 5m Communication Cable to connect Thermal Cycler with PC, 1x Serial Port Adapter, 4x Communication Cables to connect Thermal Cycler with Thermal Cycler. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 4 of 23

5 Networking Software Expansion Kit for GeneAmp PCR Each 66,00 System 9700 Includes: 5 x Communication Cables to connect Thermal Cycler with Thermal Cycler. Disposables MicroAmp Disposables for the GeneAmp PCR Systems 9700, 9600, 2700 and 2400 C4 N MicroAmp Reaction Tubes with Caps 1000/Pkg 137,00 C4 N Bulkpack MicroAmp Reaction Tubes with Caps 10000/Pkg 1.250,00 C4 N MicroAmp Colored Reaction Tubes with Caps 1000/Pkg 145,00 C4 N MicroAmp Autoclaved Reaction Tubes with Caps 1000/Pkg 270,00 C4 N MicroAmp Reaction Tubes, 0.2 ml, without Caps 2000/Pkg 270,00 C4 N Bulkpack MicroAmp Reaction Tubes without Caps 10000/Pkg 1.250,00 C4 N MicroAmp Colored Reaction Tubes without Caps 1000/Pkg 137,00 C4 N MicroAmp 8 Strip Reaction Tubes, 0.2 ml, 1000/Pkg 132, Strips C4 N MicroAmp Caps, 8 Caps/Strip 300 Strips/Pkg 104,00 C4 N Bulkpack MicroAmp Caps, 8 Caps/Strip 1500 Strips/Pkg 415,00 C4 N MicroAmp 8 Strip Colored Reaction Tubes 125 Strips/Pkg 132,00 C4 N MicroAmp Colored Caps, 8 Caps/Strip 300 Strips/Pkg 111,00 C4 N MicroAmp Centrifuge Adapter Each 12,00 N MicroAmp Cap Installing Tool Each 36,00 C MicroAmp Multi Removal Tool Each 21,00 Multi-functional tool. Used for removing Microplates from a Thermal Cycler sample block; removing strip caps from Microplates and Tray/Retainer assemblies; and opening MicroAmp or GeneAmp tubes. MicroAmp Disposables for the 96-Well GeneAmp PCR Systems 9700, 9600 and 2700 C4 N MicroAmp 96-Well Reaction Plate (no barcode) 10/Pkg 76,00 C Bulkpack 96-Well Reaction Plate (50x N ) 500/Pkg 3.050,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 5 of 23

6 C4 N MicroAmp Caps, 12 Caps/Strip 200 Strips/Pkg 104,00 C4 N Bulkpack MicroAmp Caps, 12 Caps/Strip 1000 Strips/Pkg 415,00 C4 N MicroAmp Colored Caps, 12 Caps/Strip 200 Strips/Pkg 111,00 C4 N MicroAmp 96-Well Full Plate Cover 5/Pkg 104,00 C MicroAmp 9700/9600 Reaction Tubes/Tray/Retainer 20/Pkg 540,00 Assembly C Bulkpack MicroAmp 9700/9600 Reaction 100/Pkg 2.080,00 Tubes/Tray/Retainer Assembly C Bulkpack MicroAmp 9700/9600 Reaction 1000/Pkg ,00 Tubes/Tray/Retainer Assembly C Splash Free Support Base 10/Pkg 83,00 For use on GeneAmp 9600, 9700 thermal cyclers and ABI PRISM 7700 Sequence Detection System; needs retainer to hold 0.2 ml tubes. Replaces MicroAmp Base N C4 N MicroAmp 96-Well Support Base 10/Pkg 69,00 C MicroAmp Tray/Retainer Set for 96 Tubes 10/Pkg 69,00 For use with single or strip optical tubes on the ABI PRISM 7000, 7700 and GeneAmp 5700 Sequence Detection Systems, and 310 Autosampler. C4 N MicroAmp 9600/9700 Tray for Tubes with Caps 10/Pkg 55,00 MicroAmp Disposables for the GeneAmp PCR System 2400 C4 N MicroAmp 2400 Base 16/Pkg 55,00 C4 N MicroAmp 2400 Tray/Retainer Set 16/Pkg 69,00 C4 N MicroAmp 2400 Tray for Tubes with Caps 16/Pkg 55,00 C4 N MicroAmp 2400 Full Plate Cover 5/Pkg 48,00 MicroAmp Disposables for the Dual 384-Well GeneAmp PCR System 9700 C ABI PRISM 384-well Clear Optical Reaction Plate 50/Pkg 310,00 with Barcode (code 128) C ABI PRISM 384-Well Clear Optical Reaction Plate 500/Pkg 2.620,00 with Barcode (code 128) Includes: 10 x P/N europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 6 of 23

7 C MicroAmp Clear Adhesive Film 100/Pkg 127,00 GeneAmp Disposables for 60-Well GeneAmp PCR System 9700 and DNA Thermal Cycler 480 C4 N GeneAmp PCR Reaction Tubes 2000/Pkg 136,00 C4 N GeneAmp Thin-Walled Reaction Tube with Flat Cap 1000/Pkg 132,00 C4 N GeneAmp Thin-Walled Reaction Tubes with Domed Cap 2000/Pkg 380,00 C4 N GeneAmp Autoclaved Thin-Walled Reaction Tubes 1000/Pkg 215,00 PCR Enzymes AmpliTaq Gold DNA Polymerase C AmpliTaq Gold PCR Master Mix 200 Rxs 320,00 Supplied at a 2x concentration. Includes 250 U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 5ml 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 200 x 50µl reactions, Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold PCR Master Mix 10 x 200 Rxs 2.880,00 Includes 10 x 250U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 2000 x 50µl reactions and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold PCR Master Mix 2000 Rxs 2.590,00 Includes 2500 U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 50ml 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 2000 x 50µl reactions and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 7 of 23

8 C AmpliTaq Gold DNA Polymerase, with Gold Buffer 250 Units 214,00 Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer (500mM KCl, 150mM Tris/HCl ph 8.0) and 25mM MgCl 2 solution for 200 x 50µl reactions. C AmpliTaq Gold DNA Polymerase, with Gold Buffer 1000 Units 750,00 Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for 800 x 50µl reactions. C Six Paq AmpliTaq GOLD DNA Polymerase, 6 x 250 Units 1.060,00 with Gold Buffer Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for 1200 x 50µl reactions. C Twelve Paq AmpliTaq Gold DNA Polymerase, with 12 x 250 Units 1.910,00 Gold Buffer Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for 2400 x50 µl reactions. C AmpliTaq Gold DNA Polymerase, with Gold Buffer 5 x 1000 Units 2.850,00 Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for 4000 x 50µl reactions. C AmpliTaq Gold DNA Polymerase, with Gold Buffer 25 x 1000 Units ,00 Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for x 50µl reactions. C AmpliTaq Gold DNA Polymerase, Bulk Pack, with 1 x Units ,00 Gold Buffer Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for x 50µl reactions. C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 250 Units 214,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 250 Units 214,00 C1 N Six Paq AmpliTaq Gold DNA Polymerase, with 6 x 250 Units 1.060,00 Buffer I C1 N Six Paq AmpliTaq Gold DNA Polymerase, with 6 x 250 Units 1.060,00 Buffer II C1 N Twelve Paq AmpliTaq Gold DNA Polymerase, with 12 x 250 Units 1.910,00 Buffer I europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 8 of 23

9 C1 N Twelve Paq AmpliTaq Gold DNA Polymerase, with 12 x 250 Units 1.910,00 Buffer II C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 1000 Units 750,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 1000 Units 750,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 5 x 1000 Units 2.850,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 5 x 1000 Units 2.850,00 C1 N AmpliTaq Gold DNA Polymerase 1 x Units ,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 1 x Units ,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 1 x Units ,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 25 x 1000 Units ,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 25 x 1000 Units ,00 AmpliTaq Gold DNA Polymerase Buffers C GeneAmp 10x PCR Gold Buffer and MgCl ml 27,00 Solution 500mM KCl, 150mM Tris/HCl ph 8.0 and separate 25mM MgCl 2 solution for 200 x 50µl reactions. C GeneAmp 10x PCR Gold Buffer and MgCl 2 6 x 1.5 ml 81,00 Solution, 6 set For 1200 x 50µl reactions. C GeneAmp 10x PCR Gold Buffer and MgCl ml 202,00 Solution, bulk set For x 50µl reactions. C3 N GeneAmp 10x PCR Buffer I 1.5 ml 27,00 500mM KCl, 100mM Tris/HCl ph 8.3, 15mM MgCl 2 and 0,01% (w/v) gelatin. C3 N GeneAmp 10x PCR Buffer I 6 x 1.5 ml 81,00 C3 N GeneAmp 10x PCR Buffer I 150 ml 202,00 C3 N GeneAmp 10x PCR Buffer II and MgCl ml 27,00 Solution 500mM KCl, 100mM Tris/HCl ph 8.3 and separate 25mM MgCl 2. C3 N GeneAmp 10x PCR Buffer II and MgCl 2 6 x 1.5 ml 81,00 Solution europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 9 of 23

10 C3 N GeneAmp 10x PCR Buffer II and MgCl ml 202,00 Solution AmpliTaq DNA Polymerase C2 N AmpliTaq DNA Polymerase, with Buffer I 250 Units 209,00 Includes 10x GeneAmp PCR Buffer I (500mM KCl, 100mM Tris/HCl ph 8.3, 15mM MgCl 2 and 0,01% (w/v) gelatin). C2 N AmpliTaq DNA Polymerase, with Buffer II 250 Units 209,00 Includes 10x GeneAmp PCR Buffer II (500mM KCl, 100mM Tris/HCl ph 8.3) and 25mM MgCl 2 solution. C2 N AmpliTaq DNA Polymerase, with Buffer I 1000 Units 735,00 C2 N AmpliTaq DNA Polymerase, with Buffer II 1000 Units 735,00 C2 N Six Paq AmpliTaq DNA Polymerase, with Buffer I 6 x 250 Units 1.040,00 C2 N Six Paq AmpliTaq DNA Polymerase, with Buffer II 6 x 250 Units 1.040,00 C2 N Twelve Paq AmpliTaq DNA Polymerase with Buffer I 12 x 250 Units 1.880,00 C2 N Twelve Paq AmpliTaq DNA Polymerase, with Buffer II 12 x 250 Units 1.880,00 C2 N AmpliTaq DNA Polymerase, with Buffer I 5 x 1000 Units 2.800,00 C2 N AmpliTaq DNA Polymerase, with Buffer II 5 x 1000 Units 2.800,00 C2 N AmpliTaq DNA Polymerase, with Buffer I 5 x 5000 Units ,00 C2 N AmpliTaq DNA Polymerase, with Buffer II 5 x 5000 Units ,00 AmpliTaq DNA Polymerase Buffers C3 N GeneAmp 10x PCR Buffer I 1.5 ml 27,00 500mM KCl, 100mM Tris/HCl ph 8.3, 15mM MgCl 2 and 0,01% (w/v) gelatin. C3 N GeneAmp 10x PCR Buffer I 6 x 1.5 ml 81,00 C3 N GeneAmp 10x PCR Buffer I 150 ml 202,00 C3 N GeneAmp 10x PCR Buffer II and MgCl ml 27,00 Solution 500mM KCl, 100mM Tris/HCl ph 8.3 and separate 25mM MgCl 2. C3 N GeneAmp 10x PCR Buffer II and MgCl 2 6 x 1.5 ml 81,00 Solution europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 10 of 23

11 C3 N GeneAmp 10x PCR Buffer II and MgCl ml 202,00 Solution Special PCR Enzymes C AmpliTaq Gold DNA Polymerase, LD, Kit and Protocol 250 Units 255,00 Further purified AmpliTaq Gold DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Includes Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold DNA Polymerase, LD, Kit 250 Units 255,00 Further purified AmpliTaq Gold DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Protocol P/N can be downloaded from the internet as PDF file at: C AmpliTaq Gold LD DNA Polymerase, Kit and Protocol 1000 Units 910,00 Further purified AmpliTaq Gold DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Includes Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold DNA Polymerase, LD, Kit 1000 Units 910,00 Further purified AmpliTaq Gold DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Protocol P/N can be downloaded from the internet as PDF file at: C2 N AmpliTaq DNA Polymerase, LD with Buffer I 250 Units 236,00 Further purified AmpliTaq DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna per 5 Unit aliquot. Sufficient for 200 reactions at 5 Units / 50µl reaction. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 11 of 23

12 C2 N AmpliTaq DNA Polymerase, LD with Buffer II 250 Units 236,00 Further purified AmpliTaq DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Sufficient for 200 reactions at 5 Units / 50µl reaction. C2 N AmpliTaq DNA Polymerase, Stoffel Fragment 1000 Units 265,00 Modified form of AmpliTaq DNA Polymerase (10U/µl) without exonuclease activity. Includes 10x Stoffel Buffer (100mM KCl, 100mM Tris/HCl ph 8.3) and separate 25mM MgCl 2. Sufficient for 200 reactions at 5 Units / 50µl reaction. C2 N Six Paq AmpliTaq DNA Polymerase, Stoffel Fragment 6 x 1000 Units 1.310,00 Modified form of AmpliTaq DNA Polymerase (10U/µl) without exonuclease activity. Includes 10x Stoffel Buffer (100mM KCl, 100mM Tris/HCl ph 8.3) and separate 25mM MgCl 2. Sufficient for 1200 reactions at 5 Units / 50µl reaction. C2 N rtth DNA Polymerase, XL and XL Buffer II 400 Units 390,00 Blend of rtth DNA Polymerase and Tli to provide optimized amounts of both 5-3 and 3-5 nuclease activity for high yields of long PCR products. Includes sufficient enzyme and buffer for 200 XL PCR amplifications, using 2 Units / 50µl reactions. C2 N Six Paq rtth DNA Polymerase, XL and XL Buffer II 6 x 400 Units 1.950,00 Includes sufficient enzyme and buffer for 1200 XL PCR amplifications, using 2 Units / 50 µl reactions. GeneAmp PCR Kits C GeneAmp Gold PCR Reagent Kit 100 Rxn 163,00 Includes 25µl AmpliTaq Gold DNA Polymerase for automated Hot Start PCR, 10x PCR Gold Buffer, 25mM MgCl 2 solution and dntp blend for 100 x 50µl reactions and Lambda Multiplex Control DNA, primers and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 12 of 23

13 C3 N GeneAmp PCR Reagent Kit with AmpliTaq DNA 200 Rxn 465,00 Polymerase Includes 250 U/50µl AmpliTaq DNA Polymerase, 1.5ml GeneAmp 10x PCR Buffer I, 4 x 320µl 10mM GeneAmp dntps for 200 x 50µl reactions and GeneAmp Lambda Control Reagents. C3 N GeneAmp PCR Core Reagents 200 Rxn 305,00 Includes 250 U/50 µl AmpliTaq DNA Polymerase, 1.5ml GeneAmp 10x PCR Buffer II, 1.5ml 25mM MgCl 2 and 4 x 320µl 10mM GeneAmp dntps for 200 x 50µl reactions. C AmpliTaq Gold PCR Master Mix 200 Rxs 320,00 Supplied at a 2x concentration. Includes 250 U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 5ml 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 200 x 50µl reactions, Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold PCR Master Mix 10 x 200 Rxs 2.880,00 Includes 10 x 250U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 2000 x 50µl reactions and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold PCR Master Mix 2000 Rxs 2.590,00 Includes 2500 U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 50ml 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 2000 x 50µl reactions and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 13 of 23

14 GeneAmp High Fidelity PCR Systems C GeneAmp High Fidelity PCR System 40 Rxn 114,00 Includes 100 U GeneAmp High Fidelity Enzyme Mix, GeneAmp High Fidelity 10 X PCR Buffer with 15mM MgCl 2, GeneAmp High Fidelity 10 X PCR Buffer without MgCl 2, MgCl 2 solution, 25mM; Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C GeneAmp High Fidelity PCR System 200 Rxn 540,00 Includes 2 x 250 U GeneAmp High Fidelity Enzyme Mix, GeneAmp High Fidelity 10 X PCR Buffer with 15mM MgCl 2, GeneAmp High Fidelity 10 X PCR Buffer without MgCl 2, MgCl 2 solution, 25mM; Protocol P/N The protocol can also be downloaded from the internet as PDF file at: GeneAmp XL PCR Kits C3 N GeneAmp XL PCR Kit 50 Rxn 335,00 PCR kit for extra long targets up to 40kb. Contains Blend of rtth DNA Polymerase and Tli to provide optimized polymerase and proofreading activities. Includes sufficient enzyme and buffer for 100 XL PCR amplifications, using 2 Units / 50µl reactions and Lambda Control Reagents. C3 N GeneAmp XL PCR Kit 100 Rxn 640,00 Includes sufficient enzyme and buffer for 200 XL PCR amplifications, using 2 Units / 50µl reactions and Lambda Control Reagents. PCR Kits/Contamination Control C3 N GeneAmp PCR Carry-over Prevention Kit 200 Rxn 202,00 Includes 100µl AmpErase TM Uracil N-glycosylase, 1 Unit / µl; 320µl 20mM dutp for 200 x 50µl reactions. C2 N AmpErase Uracil N-glycosylase (UNG), 100 Units 168,00 1 Unit / µl europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 14 of 23

15 C3 N GeneAmp dntp Blend, 12.5mM with dutp 1ml 101,00 Single vial blend, contains each datp, dctp, dgtp at 2.5mM and dutp at 5mM for 200 x 50µl reactions. RNA PCR Kits C GeneAmp AccuRT RNA PCR Kit with Controls 100 Rxn 590,00 Kit for highly accurate one-step RT-PCR. Includes: GeneAmp AccuRT enzyme (3.75 U/µL, 200 µl), 1.0 ml 5X AccuRT Buffer, 1.5 ml 25 mm Mg(OAc) 2, Control RNA (paw109) and primers sufficient for 50 reactions, 320 µl (10 mm) each of datp, dctp, dgtp and dttp, Protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C GeneAmp AccuRT Hot Start RNA-PCR Kit with 100 Rxn 730,00 Controls Kit for highly accurate one-step RT-PCR with hot start. Includes: GeneAmp AccuRT Hot Start enzyme (3.75 U/µL, 200 µl), 1.0 ml 5X AccuRT Buffer, 1.5 ml 25 mm Mg(OAc) 2, Control RNA (paw109) and primers sufficient for 50 reactions, 320 µl (10 mm) each of datp, dctp, dgtp and dttp, protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C GeneAmp Gold RNA PCR Reagent Kit 100 Rxn 690,00 High Sensitivity, High Specificity, One-Step RNA PCR Kit containing MultiScribe Reverse Transcriptase, AmpliTaq Gold DNA Polymerase, RNase Inhibitor, 5x RT-PCR Buffer, 100mM DTT, MgCl 2 Solution, dntp Blend, Random Hexamers, Oligo d(t) 16, GeneAmp RNA P Control Kit and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 15 of 23

16 C GeneAmp Gold RNA PCR Core Kit 100 Rxn 470,00 Contains MultiScribe Reverse Transcriptase, AmpliTaq Gold DNA Polymerase, Rnase Inhibitor, 5x RT-PCR Buffer, 100mM DTT, MgCl 2 Solution, dntp Blend, Random Hexamers, Oligo d(t) 16 and Protocol PN The protocol can also be downloaded from the internet as PDF file at: C GeneAmp RNA PCR Control Kit 20 Rxn 255,00 Contains paw109 Control RNA, Primer DM151 and Primer DM152. C3 N GeneAmp RNA PCR Kit 100 Rxn 605,00 Contains MuLV Reverse Transcriptase, AmpliTaq DNA Polymerase, Rnase Inhibitor, 10x PCR Buffer II, 100mM DTT, MgCl 2 Solution, dntps, Random Hexamers, Oligo d(t) 16 and GeneAmp RNA PCR Control Kit. C3 N GeneAmp RNA PCR Core Kit 100 Rxn 440,00 Contains MuLV Reverse Transcriptase, AmpliTaq DNA Polymerase, Rnase Inhibitor, 10x PCR Buffer II, 100mM DTT, MgCl 2 Solution, dntps, Random Hexamers and Oligo d(t) 16. C3 N GeneAmp EZ rtth RNA PCR Kit 100 Rxn 605,00 For one step RT-PCR with high temperature reverse transcription. Contains rtth DNA Polymerase, dntps, 5x EZ Buffer, 25mM Mn(OAc) 2 Solution and GeneAmp RNA PCR Control Kit. RNA PCR Enzymes C GeneAmp AccuRT RNA PCR Enzyme 100 Rxn 380,00 Enzyme for highly accurate one-step RT-PCR. Includes: GeneAmp AccuRT enzyme (3.75 U/µL, 200 µl), 1.0 ml 5X AccuRT Buffer, 1.5 ml 25 mm Mg(OAc) 2 and protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 16 of 23

17 C GeneAmp AccuRT RNA PCR Enzyme 1000 Rxn 3.470,00 Enzyme for highly accurate one-step RT-PCR. Includes: GeneAmp AccuRT enzyme (3.75 U/µL, 200 µl), 10 X 1.0 ml 5X AccuRT Buffer, 10 X 1.5 ml 25 mm Mg(OAc) 2, protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C GeneAmp AccuRT Hot Start RNA PCR Enzyme 100 Rxn 470,00 Enzyme for highly accurate one-step RT-PCR with hot start. Includes: GeneAmp AccuRT Hot Start enzyme (3.75 U/µL, 200 µl), 1.0 ml 5XAccuRT Buffer, 1.5 ml 25 mm Mg(OAc) 2 and protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C GeneAmp AccuRT Hot Start RNA PCR Enzyme 1000 Rxn 4.330,00 Enzyme for highly accurate one-step RT-PCR with hot start. Includes: GeneAmp AccuRT Hot Start enzyme (3.75 U/µL, 200 µl), 10 X 1.0 ml 5X AccuRT Buffer, 10 X 1.5 ml 25 mm Mg(OAc) 2, protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C2 N rtth DNA Polymerase and EZ Buffer Pack 500 Units 380,00 Includes rtth DNA Polymerase, 5X EZ Buffer and 25mM Mn(OAc) 2 Solution. C2 N rtth DNA Polymerase with Buffer Pack 500 Units 345,00 Includes rtth DNA Polymerase, 10x RT and Chelating Buffers, 10mM MnCl 2 and 25mM MgCl 2 Solutions. C2 N MuLV Reverse Transcriptase 5000 Units 78,00 Recombinant Moloney Murine Leukaemia Virus Reverse Transcriptase for 100 x 20µl reactions. C3 N RNase Inhibitor, 20 Units/µl, 100 reactions 2000 Units 81,00 Recombinant protein for 100 x 20µl reactions. RNA PCR Primers C3 N Random Hexamers, 100µl, 50µM. 100 Rxn 67,00 C3 N Oligo d(t) 16 Primers, 100µl, 50µM 100 Rxn 67,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 17 of 23

18 Other Reagents and Disposables PCR dntps C3 N GeneAmp dntps 4 x 320 µl 67,00 10mM Solution of each dntp in glass-distilled water, titrated to ph 7.0 with NaOH. For 200 x 50µl reactions. C3 N GeneAmp dntp Blend with dttp 1ml 67,00 Single vial blend, contains a 10mM solution of dntp (2.5mM each of datp, dctp, dgtp and dttp) for 200 x 50µl reactions. C Six Pack GeneAmp dntp Blend, 10mM 6 x 1ml 365,00 C Twelve Pack GeneAmp dntp Blend, 10mM 12 x 1ml 645,00 C3 N GeneAmp dntp Blend, 100mM 1ml 540,00 Single vial blend, contains a 100mM solution of dntp (25mM each of datp, dctp, dgtp and dttp) for 2000 x 50µl reactions. C Twelve Pack GeneAmp dntp Blend, 100mM. 12 x 1ml 4.440,00 C3 N GeneAmp dntps with dutp 4 x 320 µl 101,00 10mM Solution of datp, dctp and dgtp and a 20mM solution of dutp. For 200 x 50µl reactions. C3 N GeneAmp dntp Blend, 12.5mM with dutp 1ml 101,00 Single vial blend, contains each datp, dctp, dgtp at 2.5mM and dutp at 5mM for 200 x 50µl reactions. AmpliWax PCR Gems and Mineral Oil C3 N AmpliWax PCR Gem Gems 135,00 Specially formulated wax beads for hot start PCR applications used in reaction volumes from 50 to 100µl. C3 N AmpliWax PCR Gem Gems 135,00 Specially formulated wax beads for hot start PCR applications used in reaction volumes from 20 to 50µl. C Mineral Oil 30 ml 20,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 18 of 23

19 PCR Control Reagents C3 N GeneAmplimer HIV-1 Control Reagents 100 Rxn 335,00 C3 N GeneAmplimer paw109 RNA, 106 copies/µl 50 µl 101,00 DNA Sequencing Kits C3 N AmpliCycle Sequencing Kit 100 Rxn 249,00 Cycle Sequencing Kit using AmpliTaq CS which contains a point mutation virtually eliminating 5-3 exonuclease activity. DNA Preparation and Separation A PrepMan Ultra TM Sample Preparation Reagent 100 Rxn 133,00 with Protocol, 20 ml Homogeneous ready-to-use solution designed for the preparation of DNA template. For bacterial and fungal templates, DNA preparations for GMO testing and other cells. 20ml vial includes Quick Start Guide and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: D Plasmid Miniprep Kit 100 Rxn 265,00 D Plasmid Miniprep Kit 500 Rxn 1.210,00 C3 N Agarose for the Separation of PCR Products 100 g 209,00 Composite Agarose blend yielding gels of high strength and flexibility. Ideal for the fine resolution of PCR products less than 1kb. In Situ PCR Consumables C3 N AmpliCover Discs 52/Pkg 78,00 C3 N AmpliCover Clips 10/Pkg 71,00 C3 N In Situ PCR Glass Slides 70/Pkg 86,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 19 of 23

20 Training TRAIN-PCRBASIC Basic PCR Training ** 1 pers / 1 day 525,00 The course is designed for anyone, with or without previous experience, striving for successful PCR. The course includes, the basic principles of the PCR process, optimisation of reaction components and conditions, primer design, quantitative PCR, RT-PCR, long PCR, alternative enzymes and troubleshooting. **Training fees do not include travel and subsistence. Ordering Information Delivery charge per order 15,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 20 of 23

21 For further information, to order or to obtain a quotation please contact : Applera Italia Via Tiepolo 18 I Monza (MI) Tel: Fax: Visit us at europe.appliedbiosystems.com and register at New Store Customer to get a complete overview of products, create your personal catalogue, see full pricing information, place orders and check your order status. PLEASE NOTE: Applied Biosystems markets products for specific uses. Please refer to the product label for the intended use statements of specific products. Applied Biosystems products are intended For Research Use Only. Not for use in diagnostic procedures. This price list supersedes all previous price lists and is subject to change without notice. This price list is only for distribution within Europe. All Prices Quoted Exclude V.A.T Applied Biosystems. All rights reserved. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 21 of 23

22 Trademarks Applied Biosystems, AmpliCover, and MicroAmp are registered trademarks and AB (Design), Applera, and PrepMan are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. AmpErase, AmpliTaq, AmpliTaq Gold, AmpliWax, and GeneAmp are registered trademarks of Roche Molecular Systems, Inc. All other trademarks are the sole property of their respective owners. PLEASE NOTE: 1. Notice to Purchaser: Authorized Thermal Cylcer The GeneAmp PCR System 2700, and the GeneAmp PCR System 9700 Base Unit, in combination with its immediately attached sample block modules,comprise an instrument that is an Authorized Thermal Cycler. The purchase price of the 2700 PCR System or the 9700 PCR System Base Base Unit includes the up-front fee component of a license under United States Patent Nos. 4,683,195, 4,683,202 and 4,965,188, owned by Roche Molecular Systems, Inc., and under corresponding claims in patents outside the United States, owned by F. Hoffmann-La Roche Ltd, covering the Polymerase Chain Reaction ("PCR") process to practice the PCR process for internal research and development using this instrument. The running royalty component of that license may be purchased from Applied Biosystems or obtained by purchasing Authorized Reagents. This instrument is also an Authorized Thermal Cycler for use with applications licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Purchase of this product does not itself convey to the purchaser a complete license or right to perform the PCR process. 2. Auto-Lid Patents are pending on the Auto-Lid mechanism. 3. Notice to Purchaser: License Disclaimer: Networking Software Purchase of this software product alone does not imply any license under any process, instrument or other apparatus, system, composition, reagent or kit rights under patent claims owned or otherwise controlled by Applera Corporation, either expressly or by estoppel. 4. Notice to Purchaser: LIMITED LABEL LICENSE: AmpliTaq and AmpliTaq Gold A license under U.S. Patents 4,683,202, 4,683,195, and 4,965,188 or their foreign counterparts, owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche), for use in research and development, has an up-front fee component and a running-royalty component. The purchase price of this product includes limited, non-transferable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction (PCR) and related processes described in said patents solely for the research and development activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up- front fee component. Rights to the up-front fee component must be obtained by the end user in order to have a complete license. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an authorized thermal cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 22 of 23

23 5. Notice to Purchaser: Disclaimer of License: Other PCR Reaction Components These products are optimized for use in the polymerase chain reaction (PCR) covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser by the purchase of these products. A license to use the PCR Process for certain research and development activities accompanies the purchase of certain Applied Biosystems reagents when used in conjunction with an authorized thermal cycler, or is available from Applied Biosystems. 6. Notice to Purchaser: LIMITED LICENSE: AmpliCycle Sequencing Kit A license under U.S. Patents 4,683,202, 4,683,195, 4,965,188, 5,407,800, 5,322,770 and 5,310,652 or their foreign counterparts, owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd ("Roche"), has an up-front fee component and a running-royalty component. The purchase price of this product includes limited, nontransferable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction ("PCR") and related processes described in said patents solely for the research and development activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up-front fee component. Rights to the up-front fee component must be obtained by the end user in order to have a complete license to use this product in the PCR process. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an Authorized Thermal Cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. Further information on purchasing licenses to practice the PCR process may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California or at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501, USA. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 23 of 23

Real-Time Quantitative PCR Instruments and Consumables

Real-Time Quantitative PCR Instruments and Consumables Index Page Sequence Detection Systems 2 Sequence Detection Systems Accessories and Softwares 3 Sequence Detection PCR Reagent Kits 4 Sequence Detection PCR Reagents 9 Sequence Detection Systems Disposables

More information

PCR Optimization: Reaction Conditions and Components Sample Volume and Reaction Tubes Vapor Barrier and Thermal Transfer Fluid Template DNA or RNA

PCR Optimization: Reaction Conditions and Components Sample Volume and Reaction Tubes Vapor Barrier and Thermal Transfer Fluid Template DNA or RNA PCR Optimization: Reaction Conditions and Components The GeneAmp PCR process is widely employed in a tremendous variety of experimental applications to produce high yields of specific DNA target sequences.

More information

Blend Taq / Blend Taq -Plus-

Blend Taq / Blend Taq -Plus- Instruction manual Blend Taq and Blend Taq -Plus- 0810 F0938K Blend Taq / Blend Taq -Plus- BTQ-101 250 U 200 reactions BTQ-201 250 U 200 reactions Contents [1] Introduction

More information

The GeneAmp PCR System 9700. Results you can trust. A PCR platform you can grow with. GeneAmp. PCR System 9700

The GeneAmp PCR System 9700. Results you can trust. A PCR platform you can grow with. GeneAmp. PCR System 9700 The GeneAmp PCR System 9700 Results you can trust. A PCR platform you can grow with. GeneAmp PCR System 9700 The GeneAmp PCR System 9700 fits your lab bench, your applications and your budget. The GeneAmp

More information

4 General PCR Methods Page

4 General PCR Methods Page Table of Contents General PCR Methods Page PCR Protocol Selection Guide...65.1 Basic PCR... 66.1.1 Hot Start PCR - The new Standard... 66.1.1.1 Reagents and Equipment Required... 66.1.1.2 General Considerations

More information

PrimeSTAR HS DNA Polymerase

PrimeSTAR HS DNA Polymerase Cat. # R010A For Research Use PrimeSTAR HS DNA Polymerase Product Manual Table of Contents I. Description...3 II. III. IV. Components...3 Storage...3 Features...3 V. General Composition of PCR Reaction

More information

PCR Product Pre-Sequencing Kit

PCR Product Pre-Sequencing Kit All goods and services are sold subject to the terms and conditions of sale of the company within USB Corporation or the group which supplies them. A copy of these terms and conditions is available on

More information

Absolute Quantification Getting Started Guide

Absolute Quantification Getting Started Guide 5 cdna Reverse Primer Oligo d(t) or random hexamer Synthesis of 1st cdna strand 3 5 cdna Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Absolute Quantification Getting Started Guide Introduction

More information

Realtime PCR Master Mix

Realtime PCR Master Mix Instruction manual Realtime PCR Master Mix 0810 F0923K Realtime PCR Master Mix Contents [1] Introduction [2] Components [3] Primer/Probe design [4] Detection [5] Specimens [6] Protocol 1. TaqMan assay

More information

CompleteⅡ 1st strand cdna Synthesis Kit

CompleteⅡ 1st strand cdna Synthesis Kit Instruction Manual CompleteⅡ 1st strand cdna Synthesis Kit Catalog # GM30401, GM30402 Green Mountain Biosystems. LLC Web: www.greenmountainbio.com Tel: 800-942-1160 Sales: Sales@ greenmountainbio.com Support:

More information

Protocol. Introduction to TaqMan and SYBR Green Chemistries for Real-Time PCR

Protocol. Introduction to TaqMan and SYBR Green Chemistries for Real-Time PCR Protocol Introduction to TaqMan and SYBR Green Chemistries for Real-Time PCR Copyright 2008, 2010 Applied Biosystems. All rights reserved. Ambion and Applied Biosystems products are for Research Use Only.

More information

Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit

Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit Product Bulletin Human Identification Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit The Quantifiler kits produce reliable and reproducible results, helping to

More information

PRODUCT BROCHURE PCR Essentials. PCR Essentials. The world s most trusted PCR solutions

PRODUCT BROCHURE PCR Essentials. PCR Essentials. The world s most trusted PCR solutions PRODUCT BROCHURE PCR Essentials PCR Essentials The world s most trusted PCR solutions Applied Biosystems helped to revolutionize molecular biology with the first commercially available thermal cyclers

More information

TaqMan Fast Advanced Master Mix. Protocol

TaqMan Fast Advanced Master Mix. Protocol TaqMan Fast Advanced Master Mix Protocol For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice. APPLIED

More information

RT-PCR: Two-Step Protocol

RT-PCR: Two-Step Protocol RT-PCR: Two-Step Protocol We will provide both one-step and two-step protocols for RT-PCR. We recommend the twostep protocol for this class. In the one-step protocol, the components of RT and PCR are mixed

More information

Reverse Transcription System

Reverse Transcription System TECHNICAL BULLETIN Reverse Transcription System Instruc ons for use of Product A3500 Revised 1/14 TB099 Reverse Transcription System All technical literature is available on the Internet at: www.promega.com/protocols/

More information

Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit

Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit USER GUIDE Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit Catalog Number 4368577, 4367659, 4367660, 4368706, 4368702, 4368708 (Master Mix) and 4368711 (RT-PCR Reagents Kit) Publication

More information

ab185916 Hi-Fi cdna Synthesis Kit

ab185916 Hi-Fi cdna Synthesis Kit ab185916 Hi-Fi cdna Synthesis Kit Instructions for Use For cdna synthesis from various RNA samples This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 1

More information

PicoMaxx High Fidelity PCR System

PicoMaxx High Fidelity PCR System PicoMaxx High Fidelity PCR System Instruction Manual Catalog #600420 (100 U), #600422 (500 U), and #600424 (1000 U) Revision B Research Use Only. Not for Use in Diagnostic Procedures. 600420-12 LIMITED

More information

Super One-step RT-PCR Kit for Virus Detection

Super One-step RT-PCR Kit for Virus Detection Super One-step RT-PCR Kit for Virus Detection For fast and sensitive one-step RT-PCR with highly specificity www.tiangen.com/en SD121221 Super One-step RT-PCR Kit Kit Contents Contents Cat. no. SD103 SD103

More information

Path-ID Multiplex One-Step RT-PCR Kit

Path-ID Multiplex One-Step RT-PCR Kit USER GUIDE Path-ID Multiplex One-Step RT-PCR Kit TaqMan probe-based multiplex one-step real-time RT-PCR detection of RNA targets Catalog Numbers 4428206, 4428207, 4440022 Publication Part Number 4440907

More information

The world s most trusted PCR solutions

The world s most trusted PCR solutions PCR Essentials The world s most trusted PCR solutions Invitrogen Applied Biosystems Invitrogen offers you the best selection of high-quality PCR products that you ve relied on for years. And now, with

More information

User Bulletin #2 ABI PRISM 7700 Sequence Detection System

User Bulletin #2 ABI PRISM 7700 Sequence Detection System User Bulletin #2 ABI PRISM 7700 Sequence Detection System December 11, 1997 (updated 10/2001) SUBJECT: Relative Quantitation of Gene Expression Introduction Amplification of an endogenous control may be

More information

Taq98 Hot Start 2X Master Mix

Taq98 Hot Start 2X Master Mix Taq98 Hot Start 2X Master Mix Optimized for 98C Denaturation Lucigen Corporation 2905 Parmenter St, Middleton, WI 53562 USA Toll Free: (888) 575-9695 (608) 831-9011 FAX: (608) 831-9012 lucigen@lucigen.com

More information

Absolute Quantitation Using Standard Curve Getting Started Guide

Absolute Quantitation Using Standard Curve Getting Started Guide Applied Biosystems 7900HT Fast Real-Time PCR System Absolute Quantitation Using Standard Curve Getting Started Guide Introduction Designing an AQ Experiment Preparing the Samples and Reaction Plate Creating

More information

SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit

SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit USER GUIDE SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit Catalog Number 4309155 (Master Mix) and 4306736 (RT-PCR Reagents Kit) Publication Part Number 4310251 Rev. G Revision Date September

More information

Reagents for PCR. Expertise in Amplification

Reagents for PCR. Expertise in Amplification Reagents for PCR Expertise in Amplification Selection Guide Hot Start Enzymes for Specific Requirements PCR Application: Routine PCR Fast PCR Hot Start Multiplex/ Sequencing Wide Variety/ Easy Access High

More information

High-Capacity cdna Reverse Transcription Kits For 200 and 1000 Reactions

High-Capacity cdna Reverse Transcription Kits For 200 and 1000 Reactions High-Capacity cdna Reverse Transcription Kits For 200 and 1000 Reactions Protocol Copyright 2006,. All rights reserved. For Research Use Only. Not for use in diagnostic procedures. Information in this

More information

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-)

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE Lucigen Corporation 2905 Parmenter St, Middleton, WI 53562 USA Toll Free: (888) 575-9695 (608)

More information

FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE.

FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE. Instruction manual KOD -Plus- 0902 F0934K KOD -Plus- Contents [1] Introduction [2] Components [3] Quality testing [4] Primer design [5] Cloning of PCR products [6] Protocol 1. Standard reaction setup 2.

More information

Real-time quantitative RT -PCR (Taqman)

Real-time quantitative RT -PCR (Taqman) Real-time quantitative RT -PCR (Taqman) Author: SC, Patti Lab, 3/03 This is performed as a 2-step reaction: 1. cdna synthesis from DNase 1-treated total RNA 2. PCR 1. cdna synthesis (Advantage RT-for-PCR

More information

Megaplex Pools For microrna Expression Analysis

Megaplex Pools For microrna Expression Analysis Megaplex Pools For microrna Expression Analysis Protocol Copyright 2008, 2010 Applied Biosystems. All rights reserved. For Research Use Only. Not for use in diagnostic procedures. Information in this document

More information

First Strand cdna Synthesis

First Strand cdna Synthesis 380PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name First Strand cdna Synthesis (Cat. # 786 812) think proteins! think G-Biosciences

More information

PicoMaxx High Fidelity PCR System

PicoMaxx High Fidelity PCR System PicoMaxx High Fidelity PCR System Instruction Manual Catalog #600420 (100 U), #600422 (500 U), and #600424 (1000 U) Revision C Research Use Only. Not for Use in Diagnostic Procedures. 600420-12 LIMITED

More information

RevertAid Premium First Strand cdna Synthesis Kit

RevertAid Premium First Strand cdna Synthesis Kit RevertAid Premium First Strand cdna Synthesis Kit #K1651, #K1652 CERTIFICATE OF ANALYSIS #K1651 Lot QUALITY CONTROL RT-PCR using 100 fg of control GAPDH RNA and GAPDH control primers generated a prominent

More information

SYBR Green Realtime PCR Master Mix -Plus-

SYBR Green Realtime PCR Master Mix -Plus- Instruction manual SYBR Green Realtime PCR Master Mix -Plus- 0810 F0925K SYBR Green Realtime PCR Master Mix -Plus- Contents QPK-212T 1mLx1 QPK-212 1mLx5 Store at -20 C, protected from light [1] Introduction

More information

MOLECULAR BIOLOGY OVERVIEW NUCLEIC ACIDS: THE BASICS

MOLECULAR BIOLOGY OVERVIEW NUCLEIC ACIDS: THE BASICS MOLECULAR BIOLOGY OVERVIEW NUCLEIC ACIDS: THE BASICS Richard L. Hodinka, Ph.D. University of South Carolina School of Medicine Greenville Greenville Health System, Greenville, SC hodinka@greenvillemed.sc.edu

More information

One Nucleus Members BIOLINE PRICING 2015

One Nucleus Members BIOLINE PRICING 2015 One Nucleus Members BIOLINE PRICING quote JAN15-1NUCLEUS-GOLD or JAN15-1NUCLEUS-SILVER see prices below for GOLD and SILVER members cannot be compbined with other offers or promotions SHIPPING CHARGES:

More information

HiPer RT-PCR Teaching Kit

HiPer RT-PCR Teaching Kit HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The

More information

Put the odds in your favor

Put the odds in your favor Reverse Transcription Put the odds in your favor SuperScript III Reverse Transcriptase Reverse Transcription Engineered for performance SuperScript III Reverse Transcriptase (RT) is engineered for reduced

More information

User Manual. CelluLyser Lysis and cdna Synthesis Kit. Version 1.4 Oct 2012 From cells to cdna in one tube

User Manual. CelluLyser Lysis and cdna Synthesis Kit. Version 1.4 Oct 2012 From cells to cdna in one tube User Manual CelluLyser Lysis and cdna Synthesis Kit Version 1.4 Oct 2012 From cells to cdna in one tube CelluLyser Lysis and cdna Synthesis Kit Table of contents Introduction 4 Contents 5 Storage 5 Additionally

More information

Oligo d(t) Primers. Product Specification. Reverse Transcriptase Primers, cdna Cloning Primers, T7 RNA Amplification Primers

Oligo d(t) Primers. Product Specification. Reverse Transcriptase Primers, cdna Cloning Primers, T7 RNA Amplification Primers Product Specification Reverse Transcriptase Primers, cdna Cloning Primers, T7 RNA Amplification Primers Oligo d(t) Primers Shipped at ambient temperature. Store at -20 o C For research use only. Not for

More information

Reference Dye N/A ROX & ROX II (as separate tubes) ROX & ROX II (as separate tubes)

Reference Dye N/A ROX & ROX II (as separate tubes) ROX & ROX II (as separate tubes) Comparison of TaKaRa s and qrt-pcr Kits SYBR Premix Ex Taq (Tli RNase H+) Premix Ex Taq (Probe qpcr) SYBR Premix Ex Taq II (Tli RNase H+) Cat.# RR420A RR390A RR820A Reactions/Kit 200 (50 µl reaction size)

More information

Certificate of Analysis

Certificate of Analysis Certificate of Analysis SMART-Seq v4 3 DE Kit Components Catalog No(s). Amount Lot Number 635042 (Not sold separately; Sold as a part of 635040) 96 rxns Specified on product label. 635043 (Not sold separately;

More information

ProtoScript Taq RT-PCR Kit

ProtoScript Taq RT-PCR Kit DNA AMPLIFICATION & PCR ProtoScript Taq RT-PCR Kit Instruction Manual NEB #E6400S Store at 20 C ProtoScript Taq RT-PCR Kit Table of Contents: Introduction... 2 Quality Controls.... 2 Important Factors

More information

Table of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3

Table of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3 Table of Contents I. Description... 2 II. Kit Components... 2 III. Storage... 2 IV. 1st Strand cdna Synthesis Reaction... 3 V. RT-PCR, Real-time RT-PCR... 4 VI. Application... 5 VII. Preparation of RNA

More information

Dengue Virus subtypes 1,2 3 and 4. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... 3 Untranslated Region (3 UTR) 150 tests

Dengue Virus subtypes 1,2 3 and 4. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... 3 Untranslated Region (3 UTR) 150 tests TM Primerdesign Ltd TM Primerdesign Ltd Dengue Virus subtypes 1,2 3 and 4 3 Untranslated Region (3 UTR) genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory

More information

Řekněte si o vzorky zdarma!

Řekněte si o vzorky zdarma! strana 1 z 6 Ceník platí v souladu s Obchodními podmínkami LAB MARK od 15.10.2015 Core Reagents for Molecular Biology www.bioline.com Řekněte si o vzorky zdarma! PCR ENZYME & MIXES DNA Polymerases - For

More information

Epstein Barr Virus (Human Herpes virus 4) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere...

Epstein Barr Virus (Human Herpes virus 4) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... TM Primerdesign Ltd TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere...

More information

Genomic DNA Clean & Concentrator Catalog Nos. D4010 & D4011

Genomic DNA Clean & Concentrator Catalog Nos. D4010 & D4011 Page 0 INSTRUCTION MANUAL Catalog Nos. D4010 & D4011 Highlights Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)dna, etc.)

More information

RNA-related Products

RNA-related Products RNA-related Products TranscriptME RNA kit: Ideal choice for obtaining high yields of full-length cdna for RT-qPCR assays Suitable for as low RNA amount as 10 pg p p Convenient, reliable and cost-effective

More information

RNA-related Products

RNA-related Products RNA-related Products TRANSCRIPTME RNA kit: Ideal choice for obtaining high yields of full-length cdna for RT-qPCR assays Suitable for as low RNA amount as 10 pg p p Convenient, reliable and cost-effective

More information

Thermal cycler comparison: the ProFlex 96-Well PCR System demonstrates consistent, reliable performance

Thermal cycler comparison: the ProFlex 96-Well PCR System demonstrates consistent, reliable performance APPLICATION NOTE ProFlex 96-Well PCR System Thermal cycler comparison: the ProFlex 96-Well PCR System demonstrates consistent, reliable performance Introduction The GeneAmp PCR System 9700 was first introduced

More information

Technical Bulletin #176. Avoiding DNA Contamination in RT-PCR

Technical Bulletin #176. Avoiding DNA Contamination in RT-PCR 1 of 7 7/12/2007 8:27 PM Shop My Account View Cart nmlkji Products nmlkj Documents Technical Resources > Help Desk > Technical Bulletins Technical Bulletin #176 Avoiding DNA Contamination in RT-PCR A frequent

More information

Essentials of Real Time PCR. About Sequence Detection Chemistries

Essentials of Real Time PCR. About Sequence Detection Chemistries Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected

More information

AmpliTaq Gold DNA Polymerase, LD Hot Start, Strong Finish

AmpliTaq Gold DNA Polymerase, LD Hot Start, Strong Finish AmpliTaq Gold DNA Polymerase, LD Hot Start, Strong Finish Protocol Copyright 2007, 2010 Applied Biosystems. All rights reserved. For Research Use Only. Not for use in diagnostic procedures. Information

More information

Agilent SureCycler 8800 SURELY BETTER PCR SOLUTIONS CHOOSING THE RIGHT THERMAL CYCLER HAS NEVER BEEN EASIER

Agilent SureCycler 8800 SURELY BETTER PCR SOLUTIONS CHOOSING THE RIGHT THERMAL CYCLER HAS NEVER BEEN EASIER Agilent SureCycler 8800 SURELY BETTER PCR SOLUTIONS CHOOSING THE RIGHT THERMAL CYCLER HAS NEVER BEEN EASIER SURELY BETTER PCR FUNCTIONALITY FOR YOUR LAB Remote access Use a web-enabled device to monitor

More information

PrimeScript RT Master Mix (Perfect Real Time) Sample

PrimeScript RT Master Mix (Perfect Real Time) Sample Cat. # RR036Q For Research Use PrimeScript RT Master Mix (Perfect Real Time) Sample Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Materials Required but not Provided...

More information

Relative Quantification Applied Biosystems 7300/7500 Real Time PCR System

Relative Quantification Applied Biosystems 7300/7500 Real Time PCR System 5 3 5 cdna Reverse Primer Oligo d(t) or random hexamer 3 5 cdna Getting Started Guide Relative Quantification Applied Biosystems 7300/7500 Real Time PCR System Introduction and Example RQ Experiment Designing

More information

MystiCq microrna cdna Synthesis Mix Catalog Number MIRRT Storage Temperature 20 C

MystiCq microrna cdna Synthesis Mix Catalog Number MIRRT Storage Temperature 20 C microrna cdna Synthesis Mix Catalog Number MIRRT Storage Temperature 20 C Product Description The microrna cdna Synthesis Mix has been designed to easily convert micrornas into cdna templates for qpcr

More information

SEQUENCING SERVICES. McGill University and Génome Québec Innovation Centre JANUARY 21, 2016. Version 3.4

SEQUENCING SERVICES. McGill University and Génome Québec Innovation Centre JANUARY 21, 2016. Version 3.4 JANUARY 21, 2016 SEQUENCING SERVICES McGill University and Génome Québec Innovation Centre Sanger Sequencing User Guide Version 3.4 Copyright 2010 McGill University and Génome Québec Innovation Centre

More information

PCR and qpcr solutions

PCR and qpcr solutions A T C C C G C A T C C C G C A T C C C G C A T C C C G C A T C C G A T A T C C C G C A T C C C G C A T C C C G C A T C C C G C A T C C A T C C C G C A T C C C G C A T C C C G C A T C C C G C A T C C G C

More information

MMLV High Performance Reverse Transcriptase

MMLV High Performance Reverse Transcriptase MMLV High Performance Reverse Transcriptase Cat. Nos. RT80110K and RT80125K Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio).

More information

RT31-020 20 rxns. RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 µl 2 x 50 µl 5 x 40 µl

RT31-020 20 rxns. RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 µl 2 x 50 µl 5 x 40 µl Components RT31-020 20 rxns RT31-050 50 rxns RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 µl 2 x 50 µl 5 x 40 µl 2x RT Master Mix (2) 200 µl 2 x 250 µl 5 x 200 µl RNase H (E. coli) 20 µl 2 x 25 µl

More information

Enzymes & Reagents. ...for PCR, Real Time PCR and RT-PCR

Enzymes & Reagents. ...for PCR, Real Time PCR and RT-PCR Enzymes & Reagents...for PCR, Real Time PCR and RT-PCR PCR DNA Polymerases EuroClone recombinant DNA Polymerases are produced and purified from E.coli. Severe and rigorous production procedures ensure

More information

Human Herpes Virus 1 (Herpes simplex type 1) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere...

Human Herpes Virus 1 (Herpes simplex type 1) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... TM Primerdesign Ltd TM Primerdesign Ltd Human Herpes Virus 1 (Herpes simplex type 1) Capsid assembly and DNA maturation gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere...

More information

GoTaq Amplification Maximum performance, flexibility, control and convenience

GoTaq Amplification Maximum performance, flexibility, control and convenience GoTaq Amplification Maximum performance, flexibility, control and convenience GoTaq products and formulations designed to improve your amplification needs Reverse Transcription GoScript Reverse Transcriptase

More information

BigDye Terminator v3.1 Cycle Sequencing Kit. Protocol. DRAFT August 27, 2002 12:32 pm, 4337035A_v3.1Title.fm

BigDye Terminator v3.1 Cycle Sequencing Kit. Protocol. DRAFT August 27, 2002 12:32 pm, 4337035A_v3.1Title.fm BigDye Terminator v3.1 Cycle Sequencing Kit Protocol August 27, 2002 12:32 pm, 4337035A_v3.1Title.fm Copyright 2002, Applied Biosystems. All rights reserved. For Research Use Only. Not for use in diagnostic

More information

Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene. genesig Advanced Kit. DNA testing

Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene. genesig Advanced Kit. DNA testing TM Primerdesign Ltd TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere...

More information

360 Master Mix. , and a supplementary 360 GC Enhancer.

360 Master Mix. , and a supplementary 360 GC Enhancer. Product Bulletin AmpliTaq Gold 360 Master Mix and 360 DNA Polymerase AmpliTaq Gold 360 Master Mix AmpliTaq Gold 360 DNA Polymerase 360 Coverage for a Full Range of Targets AmpliTaq Gold 360 Master Mix

More information

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual F- 470S 20 cdna synthesis reactions (20 µl each) F- 470L 100 cdna synthesis reactions (20 µl each) Table of contents 1. Description...

More information

Quantification of Mycobacterium Tuberculosis. 150 tests

Quantification of Mycobacterium Tuberculosis. 150 tests Quantification of Mycobacterium Tuberculosis rep 13E12 For general laboratory and research use only 150 tests Introduction to Mycobacterium Tuberculosis Mycobacterium tuberculosis is the bacterium that

More information

Agilent SureCycler 8800

Agilent SureCycler 8800 Agilent SureCycler 8800 CHOOSING THE RIGHT THERMAL CYCLER HAS NEVER BEEN EASIER SURELY BETTER PCR FUNCTIONALITY FOR YOUR LAB Remote access experiment from across the room or across town hardware purchase

More information

Real-Time PCR Vs. Traditional PCR

Real-Time PCR Vs. Traditional PCR Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives

More information

AmpliTaq Gold with GeneAmp 10X PCR Buffer or GeneAmp 10X PCR Buffer II & MgCl 2 Solution or GeneAmp 10X PCR Gold Buffer

AmpliTaq Gold with GeneAmp 10X PCR Buffer or GeneAmp 10X PCR Buffer II & MgCl 2 Solution or GeneAmp 10X PCR Gold Buffer 58000935-01.qxd 12/07/2000 10:32 AM Page 1 AmpliTaq Gold with GeneAmp 10X PCR Buffer or GeneAmp 10X PCR Buffer II & MgCl 2 Solution or GeneAmp 10X PCR Gold Buffer & MgCl 2 Solution FOR RESEARCH USE ONLY

More information

AxyPrep TM Mag PCR Clean-up Protocol

AxyPrep TM Mag PCR Clean-up Protocol AxyPrep TM Mag PCR Clean-up Protocol Intro The AxyPrep Mag PCR Clean-up kit utilizes a unique paramagnetic bead technology for rapid, high-throughput purification of PCR amplicons. Using this kit, PCR

More information

Human Herpes Virus 4 (Epstein Barr)

Human Herpes Virus 4 (Epstein Barr) Techne qpcr test Human Herpes Virus 4 (Epstein Barr) nonglycosylated membrane protein (BNRF1) gene 150 tests For general laboratory and research use only 1 Introduction to Human Herpes Virus 4 (Epstein

More information

Herculase Hotstart DNA Polymerase

Herculase Hotstart DNA Polymerase Herculase Hotstart DNA Polymerase INSTRUCTION MANUAL Catalog #600310 (100 U), #600312 (500 U), and #600314 (1000 U) Revision A.01 For In Vitro Use Only 600310-12 LIMITED PRODUCT WARRANTY This warranty

More information

POLYMERASE CHAIN REACTION (PCR)

POLYMERASE CHAIN REACTION (PCR) POLYMERASE CHAIN REACTION (PCR) The polymerase chain reaction (PCR) is a technique used to amplify specific segments of DNA that may range in size from ca. 200-2000 or more base pairs. Two recent papers

More information

Troubleshooting Sequencing Data

Troubleshooting Sequencing Data Troubleshooting Sequencing Data Troubleshooting Sequencing Data No recognizable sequence (see page 7-10) Insufficient Quantitate the DNA. Increase the amount of DNA in the sequencing reactions. See page

More information

SYBR Premix Ex Taq (Tli RNaseH Plus)

SYBR Premix Ex Taq (Tli RNaseH Plus) Cat. # RR420A For Research Use SYBR Premix Ex Taq (Tli RNaseH Plus) Product Manual Table of Contents I. Description... 3 II. Principle... 3 III. Components... 4 IV. Materials Required but not Provided...

More information

All in One First Strand cdna Synthesis Kit For reliable first strand cdna synthesis from all RNA sources

All in One First Strand cdna Synthesis Kit For reliable first strand cdna synthesis from all RNA sources All in One First Strand cdna Synthesis Kit For reliable first strand cdna synthesis from all RNA sources Cat. No. AORT 0020 Cat. No. AORT 0050 (20 synthesis reactions) (50 synthesis reactions) User Manual

More information

AmpliTaq DNA Polymerase with GeneAmp 10X PCR Buffer or GeneAmp 10X PCR Buffer II & MgCl 2 Solution

AmpliTaq DNA Polymerase with GeneAmp 10X PCR Buffer or GeneAmp 10X PCR Buffer II & MgCl 2 Solution 58000954-02.qxd 12/07/2000 10:11 AM Page 1 AmpliTaq DNA Polymerase with GeneAmp 10X PCR Buffer or GeneAmp 10X PCR Buffer II & MgCl 2 Solution FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PROCEDURES

More information

Absolute Quantitation Using Standard Curve Getting Started Guide

Absolute Quantitation Using Standard Curve Getting Started Guide 5 cdna Reverse Primer Oligo d(t) or random hexamer Synthesis of 1st cdna strand 3 5 cdna Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Absolute Quantitation Using Standard Curve Getting Started

More information

Genomic DNA Extraction Kit INSTRUCTION MANUAL

Genomic DNA Extraction Kit INSTRUCTION MANUAL Genomic DNA Extraction Kit INSTRUCTION MANUAL Table of Contents Introduction 3 Kit Components 3 Storage Conditions 4 Recommended Equipment and Reagents 4 Introduction to the Protocol 4 General Overview

More information

Fast MicroSeq D2 LSU rdna Fungal Identification Kit

Fast MicroSeq D2 LSU rdna Fungal Identification Kit Fast MicroSeq D2 LSU rdna Fungal Identification Kit Protocol Copyright 2007, 2010 Applied Biosystems. All rights reserved. For Research Use Only. Not for use in diagnostic procedures. Information in this

More information

Fundamentals of Real-Time PCR. Poupak Farahani, Ph.D. Senior Product Specialist

Fundamentals of Real-Time PCR. Poupak Farahani, Ph.D. Senior Product Specialist Fundamentals of Real-Time PCR Poupak Farahani, Ph.D. Senior Product Specialist Polymerase Chain Reaction (PCR) Unamplified DNA Strand Denaturation Primer annealing PCR Primer extension Dreischrittreaktion

More information

Hepatitis B Virus. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Core Protein Region. 150 tests.

Hepatitis B Virus. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Core Protein Region. 150 tests. TM Primerdesign Ltd TM Primerdesign Ltd Hepatitis B Virus Core Protein Region genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use

More information

Precision OneStep TM qrt-pcr Mastermix

Precision OneStep TM qrt-pcr Mastermix Precision OneStep TM qrt-pcr Mastermix Instructions for use of Primerdesign Precision OneStep TM Mastermix for real-time RT-PCR ONE OneStep MasterMix Contents Introduction 3 Kit Contents 5 Recommended

More information

Idaho Technology Inc. Kit No. HRLS-ASY Reactions Kit No. HRLS-ASY Reactions

Idaho Technology Inc.  Kit No. HRLS-ASY Reactions Kit No. HRLS-ASY Reactions Idaho Technology Inc. www.idahotech.com Kit No. HRLS-ASY-0002 100 Reactions Kit No. HRLS-ASY-0003 500 Reactions Kit Contents Quantity 1 x 100 Reactions OR 5 x 100 Reactions Description 2.5X Master Mix

More information

TaqMan MicroRNA Assays

TaqMan MicroRNA Assays TaqMan MicroRNA Assays Protocol December 23, 2005 11:22 am, mirna_title.fm Copyright 2005-6, Applied Biosystems. All rights reserved. For Research Use Only. Not for use in diagnostic procedures. Information

More information

TECHNICAL BULLETIN. REDExtract-N-Amp Seed PCR Kit. Catalog Numbers XNASS, XNAS, and XNASR

TECHNICAL BULLETIN. REDExtract-N-Amp Seed PCR Kit. Catalog Numbers XNASS, XNAS, and XNASR REDExtract-N-Amp Seed PCR Kit Catalog Numbers XNASS, XNAS, and XNASR TECHNICAL BULLETIN Product Description The REDExtract-N-Amp Seed PCR Kit contains all the reagents needed to rapidly extract and amplify

More information

illustra puretaq Ready-To-Go PCR Beads

illustra puretaq Ready-To-Go PCR Beads GE Healthcare illustra puretaq Ready-To-Go PCR Beads Product Booklet Codes: 27-9557-01 (0.2 ml tubes/plate of 96) 27-9557-02 (0.2 ml tubes/5 plates of 96) 27-9558-01 (0.5 ml tubes, 100 reactions) 27-9559-01

More information

Lyme Disease. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... RecA gene. 150 tests. Primerdesign Ltd.

Lyme Disease. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... RecA gene. 150 tests. Primerdesign Ltd. TM Primerdesign Ltd TM Primerdesign Ltd Lyme Disease RecA gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use only 1 Introduction

More information

E.Z.N.A. Plant Seed Direct PCR Kit

E.Z.N.A. Plant Seed Direct PCR Kit E.Z.N.A. Plant Seed Direct PCR Kit TQ2900-00 TQ2900-01 20 preps 100 preps June 2013 E.Z.N.A. Plant Seed Direct PCR Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Suggested

More information

ABI PRISM BigDye Primer Cycle Sequencing Ready Reaction Kit With AmpliTaq DNA Polymerase, FS

ABI PRISM BigDye Primer Cycle Sequencing Ready Reaction Kit With AmpliTaq DNA Polymerase, FS ABI PRISM BigDye Primer Cycle Sequencing Ready Reaction Kit With AmpliTaq DNA Polymerase, FS Protocol Copyright 2003, 2010 Applied Biosystems. Printed in the U.S.A. For Research Use Only. Not for use in

More information

RIBOPROTECT. RNase Inhibitor RT33-020, RT33-100

RIBOPROTECT. RNase Inhibitor RT33-020, RT33-100 RIBOPROTECT RT33-020, RT33-100 RT33-020, RT33-100 RIBOPROTECT The RIBOPROTECT is a recombinant protein isolated and purified from Escherichia coli. It inhibits ribonuclease (RNase) activity of enzymes

More information

Quantification of Hepatitis B Virus. Core Protein Region. 150 tests

Quantification of Hepatitis B Virus. Core Protein Region. 150 tests Quantification of Hepatitis B Virus Core Protein Region For general laboratory and research use only 150 tests Introduction to Hepatitis B Virus Originally known as serum hepatitis, hepatitis B has only

More information

QUANTITATIVE RT-PCR. A = B (1+e) n. A=amplified products, B=input templates, n=cycle number, and e=amplification efficiency.

QUANTITATIVE RT-PCR. A = B (1+e) n. A=amplified products, B=input templates, n=cycle number, and e=amplification efficiency. QUANTITATIVE RT-PCR Application: Quantitative RT-PCR is used to quantify mrna in both relative and absolute terms. It can be applied for the quantification of mrna expressed from endogenous genes, and

More information

BacReady TM Multiplex PCR System

BacReady TM Multiplex PCR System BacReady TM Multiplex PCR System Technical Manual No. 0191 Version 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 2 V Simplified Procedures. 2 VI Detailed Experimental

More information