Řekněte si o vzorky zdarma!

Size: px
Start display at page:

Download "Řekněte si o vzorky zdarma!"

Transcription

1 strana 1 z 6 Ceník platí v souladu s Obchodními podmínkami LAB MARK od Core Reagents for Molecular Biology Řekněte si o vzorky zdarma! PCR ENZYME & MIXES DNA Polymerases - For Regular Applications BIO MyTaq DNA Polymerase 500 units BIO MyTaq DNA Polymerase 2500 units BIO MyTaq DNA Polymerase 5000 units BIO MyTaq Mix, 2x 200 Reactions BIO MyTaq Mix, 2x 1000 Reactions BIO MyTaq Red DNA Polymerase 500 units BIO MyTaq Red DNA Polymerase 2500 units BIO MyTaq Red DNA Polymerase 5000 units BIO MyTaq Red Mix, 2x 200 Reactions BIO MyTaq Red Mix, 2x 1000 Reactions BIO BIOTAQ DNA Polymerase 500 Units Regular TAQ, Red BIO BIOTAQ DNA Polymerase 2500 Units Regular TAQ BIO BioMix, 2x 500 Reactions Ready-to-go pre-mix BIO BioMix Red, 2x 500 Reactions Ready-to-go pre-mix with red dye BIO MangoTaq DNA Polymerase 1000 Units Regular TAQ, with a two-dye reaction buffer BIO MangoTaq DNA Polymerase 2000 Units Regular TAQ, with a two-dye reaction buffer BIO MangoTaq DNA Polymerase 5000 Units Regular TAQ, with a two-dye reaction buffer BIO MangoMix, 2x 250 Reactions Ready-to-go pre-mix with red and yellow dye BIO MangoMix, 2x 1000 Reactions Ready-to-go pre-mix with red and yellow dye DNA Polymerases - For Hot-Start Applications BIO MyTaq HS DNA Polymerase 250 units BIO MyTaq HS DNA Polymerase 1000 units

2 strana 2 z 6 Ceník platí v souladu s Obchodními podmínkami LAB MARK od BIO MyTaq HS DNA Polymerase 2500 units BIO MyTaq HS Mix, 2x 200 Reactions BIO MyTaq HS Mix, 2x 1000 Reactions BIO MyTaq HS Red DNA Polymerase 250 units BIO MyTaq HS Red DNA Polymerase 1000 units BIO MyTaq HS Red DNA Polymerase 2500 units BIO MyTaq HS Red Mix, 2x 200 Reactions BIO MyTaq HS Red Mix, 2x 1000 Reactions BIO MyTaq Blood PCR Mix, 2x 100 Reactions BIO MyTaq Blood PCR Mix, 2x 250 Reactions BIO IMMOLASE DNA Polymerase 250 Units Hot-Start BIO IMMOLASE DNA Polymerase 500 Units Hot-Start BIO IMMOLASE DNA Polymerase 5000 Units Hot-Start BIO ImmoMix, 2x 500 Reactions Heat-Activated, Ready-to-go pre-mix BIO ImmoMix Red, 2x 500 Reactions Heat-Activated, Ready-to-go pre-mix with red dye DNA Polymerases - For Proof-Reading/Difficult Applications BIO Ranger DNA Polymerase 250 Units BIO Ranger DNA Polymerase 500 Units BIO Ranger DNA Polymerase 2500 Units BIO Ranger Mix, 2x 100 Reactions BIO Ranger Mix, 2x 500 Reactions BIO BIO-X-ACT Short DNA Polymerase 500 Units Short-range, difficult templates BIO BIO-X-ACT Short Mix, 2x 500 Reactions Short-range, difficult templates, Ready-to-go pre-mix DNA Polymerases - For High-Fidelity Applications BIO MyFi DNA Polymerase 250 Units BIO MyFi DNA Polymerase 500 Units BIO MyFi DNA Polymerase 2500 Units BIO MyFi Mix, 2x 100 Reactions BIO MyFi Mix, 2x 500 Reactions BIO VELOCITY DNA Polymerase 250 Units High-speed, high fidelity DNA polymerase BIO VELOCITY DNA Polymerase 500 Units High-speed, high fidelity DNA polymerase BIO ACCUZYME DNA Polymerase 250 Units High-Fidelity BIO ACCUZYME DNA Polymerase 500 Units High-Fidelity BIO ACCUZYME Mix, 2x 500 Reactions High Fidelity, Ready-to-go pre-mix DNA Polymerases - Specialised Applications BIO MyTaq Extract-PCR Kit 100 Reactions BIO MyTaq Extract-PCR Kit 500 Reactions Modifying Enzymes BIO Uracil DNA-Glycosilase (UDGase) 500 Units Removal of Uracil from DNA RNA ANALYSIS cdna Synthesis BIO Tetro cdna Synthesis Kit 30 Reactions BIO Tetro cdna Synthesis Kit 100 Reactions BIO MyTaq One-Step RT-PCR Kit 25 Reactions BIO MyTaq One-Step RT-PCR Kit 100 Reactions BIO SensiFAST cdna Synthesis Kit 50 Reactions BIO SensiFAST cdna Synthesis Kit 250 Reactions RNA Reagents BIO BioScript 10,000 Units (MMLV RNAse H minus) BIO BioScript 4 x 10,000 Units (MMLV RNAse H minus) BIO Tetro Reverse Transcriptase Enzyme 10,000 Units

3 strana 3 z 6 Ceník platí v souladu s Obchodními podmínkami LAB MARK od BIO Tetro Reverse Transcriptase Enzyme 4 x 10,000 Units BIO RiboSafe RNase Inhibitor 2500 Units Inhibition of RNases (A,B,C) BIO RiboSafe RNase Inhibitor 10,000 Units Inhibition of RNases (A,B,C) BIO Random Hexamer Primer 25µg Priming ssrna or ssdna for extension BIO Oligo (dt) 18 27µg Primer for first strand cdna synthesis Ready-to-use reagent for isolation of total RNA from cells and BIO TRIsure 100ml tissues Ready-to-use reagent for isolation of total RNA from cells and BIO TRIsure 200ml tissues BIO BIO NUCLEOTIDES Sets dntp Set, 100mM dntp Set, 100mM 4 x 25µmol, (4 x 250µl) 4 x 100µmol, (4 x 4 x 250µl) Set of ready-to-use molecular grade dntp solutions Set of ready-to-use molecular grade dntp solutions BIO dntp Set, 100mM 4 x 100µmol, (4 x 1ml) Set of ready-to-use molecular grade dntp solutions Mixes BIO dntp Mix, 10mM Final Conc. 10µmol, (1ml vol.) Ready=to-go molecular grade dntp Mix BIO dntp Mix, 10mM Final Conc. 100µmol, (10 x 1ml) Ready-to-go molecular grade dntp Mix BIO dntp Mix, 40mM Final Conc. 20µmol, (1 x 500µl) Ready=to-go molecular grade dntp Mix BIO dntp Mix, 100mM Final Conc. 50µmol, (1 x 500µl) Ready=to-go molecular grade dntp Mix BIO dntp Mix, 100mM Final Conc. 200µmol, (4 x 500µl) Ready=to-go molecular grade dntp Mix BIO dutp Mix, 50mM Final Conc. 25µmol, (1 x 500µl) Ready=to-go molecular grade dntp Mix Invididuals BIO dutp, 100mM 25µmol, (1 x 250µl) 2'-Deoxyuridine 5'Triphosphate BIO datp, 100mM 25µmol, (1 x 250µl) 2' Deoxyadenosine 5'-Triphosphate BIO dgtp, 100mM 25µmol, (1 x 250µl) 2'-Deoxyguanosine 5' -Triphosphate BIO dctp, 100mM 25µmol, (1 x 250µl) 2'-Deoxycytidine 5'- Triphosphate BIO dttp, 100mM 25µmol, (1 x 250µl) 2'-Deoxythymidine 5'-Triphosphate BIO Hydroxymethylated dctp, 100mM 25µmol, (1 x 250µl) MOLECULAR WEIGHT MARKERS Červeně označené položky jsou uvedeny s akčními cenami platnými do DNA Markers Sleva 20 %! BIO HyperLadder 1kb, formally HyperLadder I 100 Lanes BIO HyperLadder 1kb, formally HyperLadder I 200 Lanes Quantitative, ranging from 200 to10kb BIO HyperLadder 1kb, formally HyperLadder I 500 Lanes Quantitative, ranging from 200 to10kb BIO HyperLadder 50bp, formally HyperLadder II 100 Lanes BIO HyperLadder 50bp, formally HyperLadder II 200 Lanes Quantitative, from 50bp to 2kb BIO HyperLadder 50bp, formally HyperLadder II 500 Lanes Quantitative, ranging from 50bp to 2kb BIO HyperLadder 100bp, formally HyperLadder IV 100 Lanes BIO HyperLadder 100bp, formally HyperLadder IV 200 Lanes Quantitative, ranging from 100 to 1,000bp BIO HyperLadder 100bp, formally HyperLadder IV 500 Lanes Quantitative, ranging from 100 to 1,000bp BIO HyperLadder 25bp, formally HyperLadder V 100 Lanes BIO HyperLadder 25bp, formally HyperLadder V 200 Lanes Quantitative, ranging from 25 to 500bp BIO HyperLadder 25bp, formally HyperLadder V 500 Lanes Quantitative, ranging from 25 to 500bp BIO EasyLadder I 100 Lanes BIO EasyLadder I 200 Lanes Quantitative, ranging from 100bp to 2000bp BIO EasyLadder I 500 Lanes Quantitative, ranging from 100bp to 2000bp Protein Marker BIO HyperPAGE Prestained Protein Marker 500µl Loading Buffers, DNA BIO DNA Loading Buffer Blue, 5x 2 x 1ml BIO DNA Loading Buffer Red, 5x 2 x 1ml BIO DNA Loading Buffer TriColour, 5x 2 x 1ml CLONING Cloning Reagents BIO Quick-Stick Ligase 50 Reactions DNA Ligation in 5 minutes BIO Quick-Stick Ligase 100 Reactions DNA Ligation in 5 minutes

4 strana 4 z 6 Ceník platí v souladu s Obchodními podmínkami LAB MARK od Competent Cells BIO Competent Cells, Bronze Efficiency 2ml (10 x 200µl) BIO Competent Cells, Silver Efficiency 2ml (10 x 200µl) Chemically competent cells for routine cloning BIO Competent Cells, Gold Efficiency 1ml (20 x 50µl) Chemically competent cells for routine cloning BIO Competent Cells, Electrocompetent 1ml (10 x 100µl) Electrocompetent cells for routine cloning BIO BioBlue, 109 1ml (20 x 50µl) Chemically competent cells for preparation of high-quality plasmid DNA BIO Electro-SHOX 1ml (10 x 100µl) Electrocompetent cells for cloning methylated DNA and transformation of large plasmids BIO CH3-Blue 109 1ml (20 x 50µl) Chemically competent cells for cloning methylated DNA (ideal for FlipFlop Mutagenesis Kit) DNA/RNA PURIFICATION KITS DNA Kits BIO ISOLATE Fecal DNA Kit 50 preps BIO ISOLATE Fecal DNA Kit 100 preps (2 x BIO-52082) Column-free Isolation BIO SureClean Plus 1 x 5ml PCR clean-up BIO SureClean Plus 2 x 12.5ml PCR clean-up BIO ISOLATE II Plasmid Mini Kit 10 preps BIO ISOLATE II Plasmid Mini Kit 50 preps BIO ISOLATE II Plasmid Mini Kit 250 preps BIO ISOLATE II PCR & Gel Kit 10 preps BIO ISOLATE II PCR & Gel Kit 50 preps BIO ISOLATE II PCR & Gel Kit 250 preps BIO ISOLATE II Blood Kit 10 preps BIO ISOLATE II Blood Kit 50 preps BIO ISOLATE II Blood Kit 250 preps BIO ISOLATE II Genomic DNA Kit 10 preps BIO ISOLATE II Genomic DNA Kit 50 preps BIO ISOLATE II Genomic DNA Kit 250 preps BIO ISOLATE II Plant Kit 10 preps BIO ISOLATE II Plant Kit 50 preps BIO ISOLATE II Plant Kit 250 preps BIO ISOLATE II RNA Mini Kit 10 preps BIO ISOLATE II RNA Mini Kit 50 preps BIO ISOLATE II RNA Mini Kit 250 preps BIO ISOLATE II RNA Micro Kit 10 preps BIO ISOLATE II RNA Micro Kit 50 preps BIO ISOLATE II RNA Plant Kit 10 preps BIO ISOLATE II RNA Plant Kit 50 preps BIO ISOLATE II Plasmid Buffer Set 1 Set (300 preps) BIO ISOLLATE II RNA Lysis Buffer RLY 50ml BIO ISOLATE II RNA Micro Clean-up 10 preps BIO ISOLATE II RNA Micro Clean-up 50 preps BIO ISOLATE II mirna Kit 25 preps BIO ISOLATE II Plant mirna Kit 25 preps BIO ISOLATE II RNA/DNA/Protein Kit 50 preps BIO ISOLATE II Biofluids RNA Kit 50 preps BIO ISOLATE II FFPE RNA/DNA Kit 50 preps REAGENTS FOR MOLECULAR BIOLOGY Agaroses BIO Agarose, Molecular Grade 500g For DNA/RNA electrophoresis BIO Agarose, Molecular Grade 100g For DNA/RNA electrophoresis BIO Agarose Tablets, DNase/RNase Free 300g For DNA/RNA electrophoresis, No-Mess Reagents

5 strana 5 z 6 Ceník platí v souladu s Obchodními podmínkami LAB MARK od BIO X-GAL 1g For blue/white screening BIO IPTG 5g For induction BIO Proteinase K 100mg Protein digestion BIO Proteinase K 1000mg Protein digestion BIO Proteinase K Solution 20mg/ml Protein digestion BIO Proteinase K Solution 5 x 20mg/ml Protein digestion BIO Co-Precipitant, Pink 1.5ml, 5mg/ml Visible DNA Pellets PCR Buffers & Additives BIO NH4 Buffer, 10x 3 x 1.2ml BIO MgCl2 Solution, 50mM 3 x 1.2ml BIO MangoTaq Buffer 5x, Colourless 10ml BIO MangoTaq Buffer 5x, Coloured 10ml BIO Hi-Fi Reaction Buffer, 5x 2 x 1.5ml BIO MyTaq reaction Buffer Colourless, 5x 4 x 1ml Buffer for use with MyTaq DNA Polymerase BIO MyTaq Reaction Buffer Red, 5x 4 x 1ml Buffer for use with MyTaq Red DNA Polymerase BIO RANGER Reaction Buffer, 5x 3 x 1.25ml BIO MyFi Reaction Buffer, 5x 3 x 1.25ml Genomic DNA & Controls BIO Human Genomic DNA 200ng/µl Highly-purified genomic DNA for southern blotting, library construction and as a control template BIO DNA Extraction Control 670, (Red Cap) 500 rxns BIO DNA Extraction Control 670, (Red Cap) 2000 rxns BIO DNA Extraction Control 560 (Orange Cap) 500 rxns BIO DNA Extraction Control 560 (Orange Cap) 2000 rxns BIO DNA Extraction Control 610 (Yellow Cap) 500 rxns BIO DNA Extraction Control 610 (Yellow Cap) 2000 rxns BIO RNA Extraction Control Reactions BIO RNA Extraction Control Reactions BIO RNA Extraction Control Reactions BIO RNA Extraction Control Reactions BIO RNA Extraction Control Reactions BIO RNA Extraction Control Reactions BIO RNA Extraction Control Reactions BIO RNA Extraction Control Reactions New Generation DNA Kits BIO SensiFAST SYBR Hi-ROX Kit 200 rxns 2ml BIO SensiFAST SYBR Hi-ROX Kit 500 rxns 5ml BIO SensiFAST SYBR Hi-ROX Kit 2000 rxns 20ml BIO SensiFAST SYBR Lo-ROX Kit 200 rxns 2ml BIO SensiFAST SYBR Lo-ROX Kit 500 rxns 5ml BIO SensiFAST SYBR Lo-ROX Kit 2000 rxns 20ml BIO SensiFAST SYBR No-ROX Kit 200 rxns 2ml BIO SensiFAST SYBR No-ROX Kit 500 rxns 5ml BIO SensiFAST SYBR No-ROX Kit 2000 rxns 20ml BIO SensiFAST SYBR & Fluorescein Kit 200 rxns 2ml BIO SensiFAST SYBR & Fluorescein Kit 500 rxns 5ml BIO SensiFAST SYBR & Fluorescein Kit 2000 rxns 20ml BIO SensiFAST Probe Hi-ROX Kit 200 rxns 2ml BIO SensiFAST Probe Hi-ROX Kit 500 rxns 5ml BIO SensiFAST Probe Hi-ROX Kit 2000 rxns 20ml BIO SensiFAST Probe Lo-ROX Kit 200 rxns 2ml

6 strana 6 z 6 Ceník platí v souladu s Obchodními podmínkami LAB MARK od BIO SensiFAST Probe Lo-ROX Kit 500 rxns 5ml BIO SensiFAST Probe Lo-ROX Kit 2000 rxns 20ml BIO SensiFAST Probe No-ROX Kit 200 rxns 2ml BIO SensiFAST Probe No-ROX Kit 500 rxns 5ml BIO SensiFAST Probe No-ROX Kit 2000 rxns 20ml BIO SensiFAST Genotyping Lo-ROX Mix 200 rxns BIO SensiFAST Genotyping Lo-ROX Mix 500 rxns BIO SensiFAST Genotyping Lo-ROX Mix 2000 rxns BIO SensiFAST Genotyping Hi-ROX Mix 200 rxns BIO SensiFAST Genotyping Hi-ROX Mix 500 rxns BIO SensiFAST Genotyping Hi-ROX Mix 2000 rxns DNA Kits BIO SensiMix II Probe No-ROX Kit 500 rxn BIO SensiMix II Probe No-ROX Kit 2000 rxn BIO SensiMix II Probe Hi-ROX Kit 500 rxn BIO SensiMix II Probe Hi-ROX Kit 2000 rxn BIO SensiMix II Probe Lo-ROX Kit 500 rxn BIO SensiMix II Probe Lo-ROX Kit 2000 rxn QT SensiMix SYBR No-ROX Kit 500 rxn 12.5ml QT SensiMix SYBR No-ROX Kit 2000 rxn 50ml QT SensiMix SYBR Low-ROX Kit 500 rxn 12.5ml QT SensiMix SYBR Low-ROX Kit 2000 rxn 50ml QT SensiMix SYBR Hi-ROX Kit 500 rxn 12.5ml QT SensiMix SYBR Hi-ROX Kit 2000 rxn 50ml QT SensiMix SYBR & Fluorescein Kit 500 rxn 12.5ml QT SensiMix SYBR & Fluorescein Kit 2000 rxn 50ml New Generation RNA Kits BIO SensiFAST SYBR No-ROX One-Step Kit 100 rxns 1ml BIO SensiFAST SYBR No-ROX One-Step Kit 500 rxns 5ml BIO SensiFAST SYBR Hi-ROX One-Step Kit 100 rxns 1ml BIO SensiFAST SYBR Hi-ROX One-Step Kit 500 rxns 5ml BIO SensiFAST SYBR Lo-ROX One-Step Kit 100 rxns 1ml BIO SensiFAST SYBR Lo-ROX One-Step Kit 500 rxns 5ml BIO SensiFAST SYBR & Fluorescein One-Step Kit 100 rxns 1ml BIO SensiFAST SYBR & Fluorescein One-Step Kit 500 rxns 5ml BIO SensiFAST Probe No-ROX One-Step Kit 100 rxns 1ml BIO SensiFAST Probe No-ROX One-Step Kit 500 rxns 5ml BIO SensiFAST Probe Hi-ROX One-Step Kit 100 rxns 1ml BIO SensiFAST Probe Hi-ROX One-Step Kit 500 rxns 5ml BIO SensiFAST Probe Lo-ROX One-Step Kit 100 rxns 1ml BIO SensiFAST Probe Lo-ROX One-Step Kit 500 rxns 5ml Next Generation HRM Kits BIO SensiFAST HRM 200 rxns 2ml BIO SensiFAST HRM 500 rxns 5ml BIO SensiFAST HRM 2000 rxns 20ml Epigenetics BIO EPIKTM Amplification Kit 200 Rn BIO EPIKTM Amplification Kit 500 Rn BIO SensiMix II Probe No-ROX Kit 500 rxn BIO SensiMix II Probe No-ROX Kit 2000 rxn

Reverse Transcription System

Reverse Transcription System TECHNICAL BULLETIN Reverse Transcription System Instruc ons for use of Product A3500 Revised 1/14 TB099 Reverse Transcription System All technical literature is available on the Internet at: www.promega.com/protocols/

More information

Genomic DNA Clean & Concentrator Catalog Nos. D4010 & D4011

Genomic DNA Clean & Concentrator Catalog Nos. D4010 & D4011 Page 0 INSTRUCTION MANUAL Catalog Nos. D4010 & D4011 Highlights Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)dna, etc.)

More information

HiPer RT-PCR Teaching Kit

HiPer RT-PCR Teaching Kit HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The

More information

CompleteⅡ 1st strand cdna Synthesis Kit

CompleteⅡ 1st strand cdna Synthesis Kit Instruction Manual CompleteⅡ 1st strand cdna Synthesis Kit Catalog # GM30401, GM30402 Green Mountain Biosystems. LLC Web: www.greenmountainbio.com Tel: 800-942-1160 Sales: Sales@ greenmountainbio.com Support:

More information

RevertAid Premium First Strand cdna Synthesis Kit

RevertAid Premium First Strand cdna Synthesis Kit RevertAid Premium First Strand cdna Synthesis Kit #K1651, #K1652 CERTIFICATE OF ANALYSIS #K1651 Lot QUALITY CONTROL RT-PCR using 100 fg of control GAPDH RNA and GAPDH control primers generated a prominent

More information

New products in the UD- GenoMed Ltd.'s offer!

New products in the UD- GenoMed Ltd.'s offer! Plastic prducts: New prducts in the UD- GenMed Ltd.'s ffer! All assrtments f the SARSTEDT plastic prducts Cnsumable plastic labratry tls fr cell and tissue culture Bld cllectin and diagnstic prducts Medical

More information

Table of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3

Table of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3 Table of Contents I. Description... 2 II. Kit Components... 2 III. Storage... 2 IV. 1st Strand cdna Synthesis Reaction... 3 V. RT-PCR, Real-time RT-PCR... 4 VI. Application... 5 VII. Preparation of RNA

More information

PrimeSTAR HS DNA Polymerase

PrimeSTAR HS DNA Polymerase Cat. # R010A For Research Use PrimeSTAR HS DNA Polymerase Product Manual Table of Contents I. Description...3 II. III. IV. Components...3 Storage...3 Features...3 V. General Composition of PCR Reaction

More information

RT-PCR: Two-Step Protocol

RT-PCR: Two-Step Protocol RT-PCR: Two-Step Protocol We will provide both one-step and two-step protocols for RT-PCR. We recommend the twostep protocol for this class. In the one-step protocol, the components of RT and PCR are mixed

More information

5PCR, real-time PCR, reverse transcription, and cloning

5PCR, real-time PCR, reverse transcription, and cloning 5PCR, real-time PCR, reverse transcription, and cloning 166 www.qiagen.com QIAGEN Product Guide 2007 PCR, real-time PCR, reverse transcription, and cloning 5 5.1 PCR and RT-PCR www.qiagen.com/pg/pcr Selection

More information

PCR Instruments and Consumables

PCR Instruments and Consumables Index Page GeneAmp PCR Instrument Systems 2 GeneAmp PCR System 9700 Components 3 Accessories and Software for GeneAmp PCR Instrument Systems 4 Disposables 5 PCR Enzymes 7 GeneAmp PCR Kits 12 RNA PCR Kits

More information

All-in-One First-Strand cdna Synthesis Kit

All-in-One First-Strand cdna Synthesis Kit All-in-One First-Strand cdna Synthesis Kit For reliable first-strand cdna synthesis from all RNA sources Cat. No. AORT-0020 (20 synthesis reactions) Cat. No. AORT-0050 (50 synthesis reactions) User Manual

More information

FastLine cell cdna Kit

FastLine cell cdna Kit 1. FastLine cell cdna Kit For high-speed preparation of first-strand cdna directly from cultured cells without RNA purification www.tiangen.com RT100701 FastLine cell cdna Kit Cat. no. KR105 Kit Contents

More information

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-)

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE Lucigen Corporation 2905 Parmenter St, Middleton, WI 53562 USA Toll Free: (888) 575-9695 (608)

More information

biotechrabbit Product Catalog

biotechrabbit Product Catalog biotechrabbit Product Catalog 2014 2015 biotechrabbit GmbH Neuendorfstr. 24a 16761 Hennigsdorf, Germany info@biotechrabbit.com www.biotechrabbit.com Office: +49 3302 20754 1 0 Fax: +49 3302 20754 1 1 Commercial

More information

mircute mirna qpcr Detection Kit (SYBR Green)

mircute mirna qpcr Detection Kit (SYBR Green) mircute mirna qpcr Detection Kit (SYBR Green) For detection of mirna using real-time RT-PCR (SYBR Green I) www.tiangen.com QP110302 mircute mirna qpcr Detection Kit (SYBR Green) Kit Contents Cat. no. FP401

More information

BacReady TM Multiplex PCR System

BacReady TM Multiplex PCR System BacReady TM Multiplex PCR System Technical Manual No. 0191 Version 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 2 V Simplified Procedures. 2 VI Detailed Experimental

More information

Taq98 Hot Start 2X Master Mix

Taq98 Hot Start 2X Master Mix Taq98 Hot Start 2X Master Mix Optimized for 98C Denaturation Lucigen Corporation 2905 Parmenter St, Middleton, WI 53562 USA Toll Free: (888) 575-9695 (608) 831-9011 FAX: (608) 831-9012 lucigen@lucigen.com

More information

First Strand cdna Synthesis

First Strand cdna Synthesis 380PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name First Strand cdna Synthesis (Cat. # 786 812) think proteins! think G-Biosciences

More information

RT31-020 20 rxns. RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 µl 2 x 50 µl 5 x 40 µl

RT31-020 20 rxns. RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 µl 2 x 50 µl 5 x 40 µl Components RT31-020 20 rxns RT31-050 50 rxns RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 µl 2 x 50 µl 5 x 40 µl 2x RT Master Mix (2) 200 µl 2 x 250 µl 5 x 200 µl RNase H (E. coli) 20 µl 2 x 25 µl

More information

User Manual. CelluLyser Lysis and cdna Synthesis Kit. Version 1.4 Oct 2012 From cells to cdna in one tube

User Manual. CelluLyser Lysis and cdna Synthesis Kit. Version 1.4 Oct 2012 From cells to cdna in one tube User Manual CelluLyser Lysis and cdna Synthesis Kit Version 1.4 Oct 2012 From cells to cdna in one tube CelluLyser Lysis and cdna Synthesis Kit Table of contents Introduction 4 Contents 5 Storage 5 Additionally

More information

Real-time quantitative RT -PCR (Taqman)

Real-time quantitative RT -PCR (Taqman) Real-time quantitative RT -PCR (Taqman) Author: SC, Patti Lab, 3/03 This is performed as a 2-step reaction: 1. cdna synthesis from DNase 1-treated total RNA 2. PCR 1. cdna synthesis (Advantage RT-for-PCR

More information

Updated: July 2005. 5' End label RNA markers 18.113 (18mer) and 44.12 (24mer) with Kinase and 32P-gamma-ATP. Gel purify labeled markers.

Updated: July 2005. 5' End label RNA markers 18.113 (18mer) and 44.12 (24mer) with Kinase and 32P-gamma-ATP. Gel purify labeled markers. RNA Cloning Method Flowchart Extract total RNA containing small RNAs. Check quality on denaturing gel with EtBr staining 5' End label RNA markers 18.113 (18mer) and 44.12 (24mer) with Kinase and 32P-gamma-ATP.

More information

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual F- 470S 20 cdna synthesis reactions (20 µl each) F- 470L 100 cdna synthesis reactions (20 µl each) Table of contents 1. Description...

More information

Biotechnology and Recombinant DNA (Chapter 9) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College

Biotechnology and Recombinant DNA (Chapter 9) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Biotechnology and Recombinant DNA (Chapter 9) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Primary Source for figures and content: Eastern Campus Tortora, G.J. Microbiology

More information

ab185916 Hi-Fi cdna Synthesis Kit

ab185916 Hi-Fi cdna Synthesis Kit ab185916 Hi-Fi cdna Synthesis Kit Instructions for Use For cdna synthesis from various RNA samples This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 1

More information

Application Guide... 2

Application Guide... 2 Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue Application Guide... 2 I. Description... 2 II. Product Components... 2 III. Materials to be Supplied

More information

Mir-X mirna First-Strand Synthesis Kit User Manual

Mir-X mirna First-Strand Synthesis Kit User Manual User Manual Mir-X mirna First-Strand Synthesis Kit User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories, Inc.

More information

SOLIDscript Solid Phase cdna Synthesis Kit Instruction Manual

SOLIDscript Solid Phase cdna Synthesis Kit Instruction Manual Toll Free: 866-252-7771 752A Lincoln Blvd. Phone: 732-469-7771 Fax: 732-469-7782 Middlesex, NJ 08846 Web: www.purebiotechllc.com SOLIDscript Solid Phase cdna Synthesis Kit Instruction Manual Product: SOLIDscript

More information

QUANTITATIVE RT-PCR. A = B (1+e) n. A=amplified products, B=input templates, n=cycle number, and e=amplification efficiency.

QUANTITATIVE RT-PCR. A = B (1+e) n. A=amplified products, B=input templates, n=cycle number, and e=amplification efficiency. QUANTITATIVE RT-PCR Application: Quantitative RT-PCR is used to quantify mrna in both relative and absolute terms. It can be applied for the quantification of mrna expressed from endogenous genes, and

More information

Global MicroRNA Amplification Kit

Global MicroRNA Amplification Kit Global MicroRNA Amplification Kit Store kit at -20 C on receipt (ver. 3-060901) A limited-use label license covers this product. By use of this product, you accept the terms and conditions outlined in

More information

GenScript BloodReady TM Multiplex PCR System

GenScript BloodReady TM Multiplex PCR System GenScript BloodReady TM Multiplex PCR System Technical Manual No. 0174 Version 20040915 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 2 V Simplified Procedures. 2 VI

More information

Real-Time Quantitative PCR Instruments and Consumables

Real-Time Quantitative PCR Instruments and Consumables Index Page Sequence Detection Systems 2 Sequence Detection Systems Accessories and Softwares 3 Sequence Detection PCR Reagent Kits 4 Sequence Detection PCR Reagents 9 Sequence Detection Systems Disposables

More information

POLYMERASES & AMPLIFICATION. OneTaq RT-PCR Kit. Instruction Manual. NEB #E5310S 30 reactions

POLYMERASES & AMPLIFICATION. OneTaq RT-PCR Kit. Instruction Manual. NEB #E5310S 30 reactions POLYMERASES & AMPLIFICATION OneTaq RT-PCR Kit Instruction Manual NEB #E5310S 30 reactions ISO 9001 Registered Quality Management ISO 14001 Registered Environmental Management ISO 13485 Registered Medical

More information

PrimeScript High Fidelity RT-PCR Kit

PrimeScript High Fidelity RT-PCR Kit For Research Use PrimeScript High Fidelity RT-PCR Kit Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Materials Required but not Provided... 4 IV. Storage... 4 V. Fidelity

More information

TIANquick Mini Purification Kit

TIANquick Mini Purification Kit TIANquick Mini Purification Kit For purification of PCR products, 100 bp to 20 kb www.tiangen.com TIANquick Mini Purification Kit (Spin column) Cat no. DP203 Kit Contents Contents Buffer BL Buffer PB Buffer

More information

Hepatitis B Virus Genemer Mix

Hepatitis B Virus Genemer Mix Product Manual Hepatitis B Virus Genemer Mix Primer Pair for amplification of HBV Specific DNA Fragment Includes Internal Negative Control Primers and Template Catalog No.: 60-2007-12 Store at 20 o C For

More information

AffinityScript QPCR cdna Synthesis Kit

AffinityScript QPCR cdna Synthesis Kit AffinityScript QPCR cdna Synthesis Kit INSTRUCTION MANUAL Catalog #600559 Revision C.01 For In Vitro Use Only 600559-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this

More information

Dongsheng Biotech Co., Ltd. was established in 2005 in Guangzhou China.

Dongsheng Biotech Co., Ltd. was established in 2005 in Guangzhou China. Dongsheng Biotech Co., Ltd. was established in 2005 in Guangzhou China. We focus on R&D and produce fundamental molecular biology reagents. In the past 5 years, Dongsheng Biotech has successfully grown

More information

Terra PCR Direct Polymerase Mix User Manual

Terra PCR Direct Polymerase Mix User Manual Clontech Laboratories, Inc. Terra PCR Direct Polymerase Mix User Manual Cat. Nos. 639269, 639270, 639271 PT5126-1 (031416) Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Avenue, Mountain

More information

All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna

All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna All-in-One TM mirna First-Strand cdna Synthesis Kit AMRT-0020 (20 RT reactions), AMRT-0060 (60 RT reactions) Used in combination

More information

Description Wako Catalog No. Page # microrna Purification Kits based on Immunoprecipitation method using antibody against Argonaute protein

Description Wako Catalog No. Page # microrna Purification Kits based on Immunoprecipitation method using antibody against Argonaute protein Wako microrna Products Wako Pure Chemical Industries, Ltd. microrna Cloning Ago IP microrna Purification Target mrna Screening micrornas (mirnas) are endogenous RNAs, approximately 22 nucleotides in length,

More information

SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit

SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit USER GUIDE SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit Catalog Number 4309155 (Master Mix) and 4306736 (RT-PCR Reagents Kit) Publication Part Number 4310251 Rev. G Revision Date September

More information

Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION

Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION Susan Carson Heather B. Miller D.Scott Witherow ELSEVIER AMSTERDAM BOSTON HEIDELBERG LONDON NEW YORK OXFORD PARIS SAN DIEGO SAN

More information

Bioline, your partner for virology research

Bioline, your partner for virology research Bioline, your partner for virology research Discover why scientists at the world s leading virological research institutes and reference laboratories*, have come to trust and depend upon the reliability

More information

DP419 RNAsimple Total RNA Kit. RNAprep pure Series. DP501 mircute mirna Isolation Kit. DP438 MagGene Viral DNA / RNA Kit. DP405 TRNzol Reagent

DP419 RNAsimple Total RNA Kit. RNAprep pure Series. DP501 mircute mirna Isolation Kit. DP438 MagGene Viral DNA / RNA Kit. DP405 TRNzol Reagent Overview of TIANGEN Products DP419 RNAsimple Total RNA Kit DP430 RNAprep pure Kit(For Cell/Bacteria) DP315/DP315-R TIANamp Virus DNA/RNA Kit DP431 RNAprep pure Kit (For Tissue) Silica-membrane Technology

More information

SYBR Green Realtime PCR Master Mix -Plus-

SYBR Green Realtime PCR Master Mix -Plus- Instruction manual SYBR Green Realtime PCR Master Mix -Plus- 0810 F0925K SYBR Green Realtime PCR Master Mix -Plus- Contents QPK-212T 1mLx1 QPK-212 1mLx5 Store at -20 C, protected from light [1] Introduction

More information

Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit

Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit USER GUIDE Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit Catalog Number 4368577, 4367659, 4367660, 4368706, 4368702, 4368708 (Master Mix) and 4368711 (RT-PCR Reagents Kit) Publication

More information

All-in-One mirna qrt-pcr Detection System Handbook

All-in-One mirna qrt-pcr Detection System Handbook All-in-One mirna qrt-pcr Detection System Handbook For quantitative detection of mature mirna All-in-One mirna First-Strand cdna Synthesis Kit Cat. No. AMRT-0020 (20 mirna reverse transcription reactions)

More information

PCR Reagents. The Highest Level of PCR Performance. PCR Enzymes RT-PCR Reagents QPCR and QRT-PCR Reagents dntps

PCR Reagents. The Highest Level of PCR Performance. PCR Enzymes RT-PCR Reagents QPCR and QRT-PCR Reagents dntps A m p l i f i c a t i o n PCR Reagents The Highest Level of PCR Performance PCR Enzymes RT-PCR Reagents QPCR and QRT-PCR Reagents dntps Stratagene Has Yo High-Fidelity Application General Cloning TA/UA

More information

MMLV High Performance Reverse Transcriptase

MMLV High Performance Reverse Transcriptase MMLV High Performance Reverse Transcriptase Cat. Nos. RT80110K and RT80125K Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio).

More information

Introduction To Real Time Quantitative PCR (qpcr)

Introduction To Real Time Quantitative PCR (qpcr) Introduction To Real Time Quantitative PCR (qpcr) SABiosciences, A QIAGEN Company www.sabiosciences.com The Seminar Topics The advantages of qpcr versus conventional PCR Work flow & applications Factors

More information

Wide range of high-quality enzymes and proteins for molecular biology

Wide range of high-quality enzymes and proteins for molecular biology Enzymes & Proteins Wide range of high-quality enzymes and proteins for molecular biology ENZYMES & PROTEINS We offer a wide range of high-quality enzymes and proteins for molecular biology including proteases,

More information

SOP Title: Multiplex-PCR check of genomic DNA isolated from FFPE tissue for its usability in array CGH analysis

SOP Title: Multiplex-PCR check of genomic DNA isolated from FFPE tissue for its usability in array CGH analysis SOP Title: Multiplex-PCR check of genomic DNA isolated from FFPE tissue for its usability in array CGH analysis The STORE processing methods were shown to be fit-for purpose for DNA, RNA and protein extraction

More information

GENOTYPING ASSAYS AT ZIRC

GENOTYPING ASSAYS AT ZIRC GENOTYPING ASSAYS AT ZIRC A. READ THIS FIRST - DISCLAIMER Dear ZIRC user, We now provide detailed genotyping protocols for a number of zebrafish lines distributed by ZIRC. These protocols were developed

More information

Recombinant DNA & Genetic Engineering. Tools for Genetic Manipulation

Recombinant DNA & Genetic Engineering. Tools for Genetic Manipulation Recombinant DNA & Genetic Engineering g Genetic Manipulation: Tools Kathleen Hill Associate Professor Department of Biology The University of Western Ontario Tools for Genetic Manipulation DNA, RNA, cdna

More information

Whole genome Bisulfite Sequencing for Methylation Analysis Preparing Samples for the Illumina Sequencing Platform

Whole genome Bisulfite Sequencing for Methylation Analysis Preparing Samples for the Illumina Sequencing Platform Whole genome Bisulfite Sequencing for Methylation Analysis Preparing Samples for the Illumina Sequencing Platform Introduction, 2 Sample Prep Workflow, 3 Best Practices, 4 DNA Input Recommendations, 6

More information

How To Write An Ipa

How To Write An Ipa LIBRARY PREPARATION NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) Instruction Manual NEB #E7300S/L 24/96 reactions Sign up for the NEBNext e-newsletter Scan this code or visit www.neb.com/

More information

Plasmid DNA, Gel Extraction & PCR Products Purification Kits. Code Description Size. BS363 EZ-10 Spin Column PCR Products Purification Kit 50 preps

Plasmid DNA, Gel Extraction & PCR Products Purification Kits. Code Description Size. BS363 EZ-10 Spin Column PCR Products Purification Kit 50 preps Plasmid DNA, Gel Extraction & PCR Products Purification Kits Code Description Size BS363 EZ-10 Spin Column PCR Products Purification Kit BS364 EZ-10 Spin Column PCR Products Purification Kit 100 preps

More information

qstar mirna qpcr Detection System

qstar mirna qpcr Detection System qstar mirna qpcr Detection System Table of Contents Table of Contents...1 Package Contents and Storage Conditions...2 For mirna cdna synthesis kit...2 For qstar mirna primer pairs...2 For qstar mirna qpcr

More information

The Techniques of Molecular Biology: Forensic DNA Fingerprinting

The Techniques of Molecular Biology: Forensic DNA Fingerprinting Revised Fall 2011 The Techniques of Molecular Biology: Forensic DNA Fingerprinting The techniques of molecular biology are used to manipulate the structure and function of molecules such as DNA and proteins

More information

Thermo Scientific Phusion Site-Directed Mutagenesis Kit #F-541

Thermo Scientific Phusion Site-Directed Mutagenesis Kit #F-541 PRODUCT INFORMATION Thermo Scientific Phusion Site-Directed Mutagenesis Kit #F-541 Lot _ Store at -20 C Expiry Date _ www.thermoscientific.com/onebio CERTIFICATE OF ANALYSIS The Phusion Site-Directed Mutagenesis

More information

Nucleic Acid Techniques in Bacterial Systematics

Nucleic Acid Techniques in Bacterial Systematics Nucleic Acid Techniques in Bacterial Systematics Edited by Erko Stackebrandt Department of Microbiology University of Queensland St Lucia, Australia and Michael Goodfellow Department of Microbiology University

More information

Reduced Representation Bisulfite Sequencing for Methylation Analysis Preparing Samples for the Illumina Sequencing Platform

Reduced Representation Bisulfite Sequencing for Methylation Analysis Preparing Samples for the Illumina Sequencing Platform Reduced Representation Bisulfite Sequencing for Methylation Analysis Preparing Samples for the Illumina Sequencing Platform Introduction, 3 Sample Prep Workflow, 4 Best Practices, 5 DNA Input Recommendations,

More information

Technical Manual No. 0173 Update Date 10112010

Technical Manual No. 0173 Update Date 10112010 TissueDirect TM Multiplex PCR System Technical Manual No. 0173 Update Date 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 3 V Simplified Procedures. 3 VI Detailed

More information

Catalogue. Catalogue. www. genemarkbio.com

Catalogue. Catalogue. www. genemarkbio.com Catalogue Catalogue 2 0 1 4 www. genemarkbio.com Company Profile Company Profile GMbiolab Co., Ltd. was founded in 2001 in Taiwan. We provide a variety of molecular biology kits and reagents, including

More information

Validating Microarray Data Using RT 2 Real-Time PCR Products

Validating Microarray Data Using RT 2 Real-Time PCR Products Validating Microarray Data Using RT 2 Real-Time PCR Products Introduction: Real-time PCR monitors the amount of amplicon as the reaction occurs. Usually, the amount of product is directly related to the

More information

ISOLATE II PCR and Gel Kit. Product Manual

ISOLATE II PCR and Gel Kit. Product Manual ISOLATE II PCR and Gel Kit Product Manual 2 Product Manual www.bioline.com/isolate PCR and Gel Kit ISOLATE II PCR and Gel Kit ISOLATE II PCR and Gel Kit 1 Kit contents 04 2 Description 04 3 Storage 04

More information

DyNAmo cdna Synthesis Kit for qrt-pcr

DyNAmo cdna Synthesis Kit for qrt-pcr DyNAmo cdna Synthesis Kit for qrt-pcr Instruction manual F- 470S Sufficient for 20 cdna synthesis reactions (20 µl each) F- 470L Sufficient for 100 cdna synthesis reactions (20 µl each) Description...

More information

Absolute Quantification Getting Started Guide

Absolute Quantification Getting Started Guide 5 cdna Reverse Primer Oligo d(t) or random hexamer Synthesis of 1st cdna strand 3 5 cdna Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Absolute Quantification Getting Started Guide Introduction

More information

HCS604.03 Exercise 1 Dr. Jones Spring 2005. Recombinant DNA (Molecular Cloning) exercise:

HCS604.03 Exercise 1 Dr. Jones Spring 2005. Recombinant DNA (Molecular Cloning) exercise: HCS604.03 Exercise 1 Dr. Jones Spring 2005 Recombinant DNA (Molecular Cloning) exercise: The purpose of this exercise is to learn techniques used to create recombinant DNA or clone genes. You will clone

More information

Při objednávce alespoň 5 stejných položek poskytneme množstevní slevu 10%. Červeně označené ceny jsou bez nákladu na dopravu do ČR.

Při objednávce alespoň 5 stejných položek poskytneme množstevní slevu 10%. Červeně označené ceny jsou bez nákladu na dopravu do ČR. strana 1 z 42 Ceník platí v souladu s Obchodními podmínkami LAB MARK od 17.6.2015 Při objednávce alespoň 5 stejných položek poskytneme množstevní slevu 10%. Červeně označené ceny jsou bez nákladu na dopravu

More information

Real-time monitoring of rolling circle amplification using aggregation-induced emission: applications for biological detection

Real-time monitoring of rolling circle amplification using aggregation-induced emission: applications for biological detection Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 215 Supplementary Information Real-time monitoring of rolling circle amplification using aggregation-induced

More information

2.1.2 Characterization of antiviral effect of cytokine expression on HBV replication in transduced mouse hepatocytes line

2.1.2 Characterization of antiviral effect of cytokine expression on HBV replication in transduced mouse hepatocytes line i 1 INTRODUCTION 1.1 Human Hepatitis B virus (HBV) 1 1.1.1 Pathogenesis of Hepatitis B 1 1.1.2 Genome organization of HBV 3 1.1.3 Structure of HBV virion 5 1.1.4 HBV life cycle 5 1.1.5 Experimental models

More information

HighPure Maxi Plasmid Kit

HighPure Maxi Plasmid Kit HighPure Maxi Plasmid Kit For purification of high pure plasmid DNA with high yields www.tiangen.com PP120109 HighPure Maxi Plasmid Kit Kit Contents Storage Cat.no. DP116 Contents RNaseA (100 mg/ml) Buffer

More information

IMBB 2013. Genomic DNA purifica8on

IMBB 2013. Genomic DNA purifica8on IMBB 2013 Genomic DNA purifica8on Why purify DNA? The purpose of DNA purifica8on from the cell/8ssue is to ensure it performs well in subsequent downstream applica8ons, e.g. Polymerase Chain Reac8on (PCR),

More information

RevertAid First Strand cdna Synthesis Kit (#K1621 for 10 reactions) COMPONENTS OF THE KIT

RevertAid First Strand cdna Synthesis Kit (#K1621 for 10 reactions) COMPONENTS OF THE KIT 3 RevertAid First Strand cdna Synthesis Kit (#K1621 for 10 reactions) Kit is designed for preparation of full-length fi rst strand cdna from RNA templates. The RevertAid fi rst strand cdna synthesis kit

More information

Biotechnology: DNA Technology & Genomics

Biotechnology: DNA Technology & Genomics Chapter 20. Biotechnology: DNA Technology & Genomics 2003-2004 The BIG Questions How can we use our knowledge of DNA to: diagnose disease or defect? cure disease or defect? change/improve organisms? What

More information

Essentials of Real Time PCR. About Sequence Detection Chemistries

Essentials of Real Time PCR. About Sequence Detection Chemistries Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected

More information

How many of you have checked out the web site on protein-dna interactions?

How many of you have checked out the web site on protein-dna interactions? How many of you have checked out the web site on protein-dna interactions? Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Find and be ready to discuss

More information

Cloning Blunt-End Pfu DNA Polymerase- Generated PCR Fragments into pgem -T Vector Systems

Cloning Blunt-End Pfu DNA Polymerase- Generated PCR Fragments into pgem -T Vector Systems Promega Notes Number 71, 1999, p. 10 Blunt-End Pfu DNA Polymerase- Generated PCR Fragments into pgem -T Vector Systems By Kimberly Knoche, Ph.D., and Dan Kephart, Ph.D. Promega Corporation Corresponding

More information

FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE.

FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE. Instruction manual for KOD FX Neo 1103 F1100K KOD FX Neo KFX-201 200 U 200 reactions Store at -20 C Contents [1] Introduction [2] Components [3] Quality testing [4] Primer design [5] Cloning of PCR products

More information

VLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J10

VLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J10 Topic J10+11: Molecular-biological methods + Clinical virology I (hepatitis A, B & C, HIV) To study: PCR, ELISA, your own notes from serology reactions Task J10/1: DNA isolation of the etiological agent

More information

STRUCTURES OF NUCLEIC ACIDS

STRUCTURES OF NUCLEIC ACIDS CHAPTER 2 STRUCTURES OF NUCLEIC ACIDS What is the chemical structure of a deoxyribonucleic acid (DNA) molecule? DNA is a polymer of deoxyribonucleotides. All nucleic acids consist of nucleotides as building

More information

Genomic DNA Extraction Kit INSTRUCTION MANUAL

Genomic DNA Extraction Kit INSTRUCTION MANUAL Genomic DNA Extraction Kit INSTRUCTION MANUAL Table of Contents Introduction 3 Kit Components 3 Storage Conditions 4 Recommended Equipment and Reagents 4 Introduction to the Protocol 4 General Overview

More information

Speed Matters - Fast ways from template to result

Speed Matters - Fast ways from template to result qpcr Symposium 2007 - Weihenstephan Speed Matters - Fast ways from template to result March 28, 2007 Dr. Thorsten Traeger Senior Scientist, Research and Development - 1 - Overview Ạgenda Fast PCR The Challenges

More information

Molecular Cloning Laboratories

Molecular Cloning Laboratories Molecular Cloning Laboratories Catalog 2012-2013 TABLE OF CONTENTS ABOUT US Established in 1998, Molecular Cloning Laboratories (MCLAB) is a leader in providing genomic research consumables and services

More information

Improved methods for site-directed mutagenesis using Gibson Assembly TM Master Mix

Improved methods for site-directed mutagenesis using Gibson Assembly TM Master Mix CLONING & MAPPING DNA CLONING DNA AMPLIFICATION & PCR EPIGENETICS RNA ANALYSIS Improved methods for site-directed mutagenesis using Gibson Assembly TM Master Mix LIBRARY PREP FOR NET GEN SEQUENCING PROTEIN

More information

restriction enzymes 350 Home R. Ward: Spring 2001

restriction enzymes 350 Home R. Ward: Spring 2001 restriction enzymes 350 Home Restriction Enzymes (endonucleases): molecular scissors that cut DNA Properties of widely used Type II restriction enzymes: recognize a single sequence of bases in dsdna, usually

More information

Troubleshooting the Single-step PCR Site-directed Mutagenesis Procedure Intended to Create a Non-functional rop Gene in the pbr322 Plasmid

Troubleshooting the Single-step PCR Site-directed Mutagenesis Procedure Intended to Create a Non-functional rop Gene in the pbr322 Plasmid Troubleshooting the Single-step PCR Site-directed Mutagenesis Procedure Intended to Create a Non-functional rop Gene in the pbr322 Plasmid Lina Jew Department of Microbiology & Immunology, University of

More information

RIBOPROTECT. RNase Inhibitor RT33-020, RT33-100

RIBOPROTECT. RNase Inhibitor RT33-020, RT33-100 RIBOPROTECT RT33-020, RT33-100 RT33-020, RT33-100 RIBOPROTECT The RIBOPROTECT is a recombinant protein isolated and purified from Escherichia coli. It inhibits ribonuclease (RNase) activity of enzymes

More information

Lecture 13: DNA Technology. DNA Sequencing. DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology

Lecture 13: DNA Technology. DNA Sequencing. DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology Lecture 13: DNA Technology DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology DNA Sequencing determine order of nucleotides in a strand of DNA > bases = A,

More information

PicoMaxx High Fidelity PCR System

PicoMaxx High Fidelity PCR System PicoMaxx High Fidelity PCR System Instruction Manual Catalog #600420 (100 U), #600422 (500 U), and #600424 (1000 U) Revision C Research Use Only. Not for Use in Diagnostic Procedures. 600420-12 LIMITED

More information

U.S. Patent No. 9,051,563 and other pending patents. Ver. 1.1.3

U.S. Patent No. 9,051,563 and other pending patents. Ver. 1.1.3 INSTRUCTION MANUAL Direct-zol RNA MiniPrep Catalog Nos. R050, R05, R05, & R053 Highlights Quick, spin column purification of high-quality (DNA-free) total RNA directly from TRIzol, TRI Reagent and other

More information

UltraClean Soil DNA Isolation Kit

UltraClean Soil DNA Isolation Kit PAGE 1 UltraClean Soil DNA Isolation Kit Catalog # 12800-50 50 preps New improved PCR inhibitor removal solution (IRS) included Instruction Manual (New Alternative Protocol maximizes yields) Introduction

More information

UltraClean Forensic DNA Isolation Kit (Single Prep Format)

UltraClean Forensic DNA Isolation Kit (Single Prep Format) UltraClean Forensic DNA Isolation (Single Prep Format) Catalog No. Quantity 14000-10 10 preps 14000-20 20 preps 14000-S 1 prep Instruction Manual F Please recycle Version: 06152009 1 Table of Contents

More information

DNA Isolation Kit for Cells and Tissues

DNA Isolation Kit for Cells and Tissues DNA Isolation Kit for Cells and Tissues for 10 isolations of 00 mg each for tissue or 5 x 10 7 cultured cells Cat. No. 11 81 770 001 Principle Starting material Application Time required Results Benefits

More information

Qubit dsdna HS Assay Kits

Qubit dsdna HS Assay Kits Qubit dsdna HS Assay Kits For use with the Qubit 2.0 Fluorometer Table 1. Contents and storage information. Material Amount Concentration Storage Stability Qubit dsdna HS Reagent (Component A) 250 µl or

More information

Taq PCR Handbook. Sample & Assay Technologies. Taq DNA Polymerase Taq PCR Core Kit Taq PCR Master Mix Kit

Taq PCR Handbook. Sample & Assay Technologies. Taq DNA Polymerase Taq PCR Core Kit Taq PCR Master Mix Kit Third Second Edition December October 2010 2005 Taq PCR Handbook Taq DNA Polymerase Taq PCR Core Kit Taq PCR Master Mix Kit For standard and specialized PCR applications with minimal optimization Sample

More information

User Bulletin #2 ABI PRISM 7700 Sequence Detection System

User Bulletin #2 ABI PRISM 7700 Sequence Detection System User Bulletin #2 ABI PRISM 7700 Sequence Detection System December 11, 1997 (updated 10/2001) SUBJECT: Relative Quantitation of Gene Expression Introduction Amplification of an endogenous control may be

More information