1 Degradation of Aspartyl Peptides Studies on the isomerization and enantiomerization of aspartic acid in model peptides by capillary electrophoresis and high performance liquid chromatography Dissertation zur Erlangung des akademischen Grades Doctor rerum naturalium (Dr. rer. nat.) vorgelegt dem Rat der Biologisch Pharmazeutischen Fakultät der Friedrich Schiller Universität Jena von Diplom Pharmazeutin Silvia De Boni geboren am 23. Juli 1975 in Conegliano (Italien)
2 Gutachter: 1. Prof. Dr. G. Scriba 2. Prof. Dr. J. Lehmann 3. Prof. Dr. G. Blaschke Tag der mündlichen Prüfung: Tag der öffentlichen Verteidigung:
3 For Jan
4 Contents I Contents 1. Introduction Instability of aspartyl peptides Analytical techniques to investigate aspartic acid degradation Aim of the work 4 2. Synthesis of reference substances Solid phase peptide synthesis of tripeptides with free terminal carboxyl group Synthesis of succinimidyl peptides 7 3. Incubation of model peptides Incubation of Phe-Asp-Gly 2 and Gly-Asp-Phe Incubation of Phe-Asu-Gly 9 4. Analysis of degradation products by capillary electrophoresis Basic principles of peptide analysis by CE Analysis by CE in achiral buffer Analysis by CE using cyclodextrins Analysis of Gly-α-L-Asp-L-Phe 2 and Gly-α-D-Asp-L-Phe Analysis of L-Phe-β-L-Asp-Gly 2, L-Phe-α-L-Asp-Gly and L-Phe-α-D-Asp-Gly Analysis of the diketopiperazine derivatives cyclo(phe-asp) and cyclo(gly-asp) Analysis of the degradation products after incubation of Phe-Asu-Gly Analysis of degradation products by RP-PLC Analysis by RP-PLC in water/acetonitrile Analysis by RP-PLC in phosphate buffer Comparison between CE and PLC Identification of degradation products by on-line mass spectrometry CE-MS/MS PLC-MS Quantification of degradation products Selection of internal standard Calibration of reference substances Precision 32
5 Contents II 8. Kinetics of degradation reactions Kinetics of the degradation of Phe-Asp-Gly 2 and Gly-Asp-Phe Incubations at p Incubations at p Kinetics of the degradation of Phe-Asu-Gly Conclusions Materials and methods Fmoc-solid phase peptide synthesis Synthesis of succinimidyl peptides Incubations Capillary electrophoresis with UV detection RP-PLC with UV detection CE-MS/MS RP-PLC-MS Calibration and validation Materials, chemicals and instrumentation General instrumentation Chemicals Zusammenfassung References Appendix Calibration of reference substances and validation of the method Calibration and validation for CE-system 1 and CE-System Calibration and validation for CE-System Calibration and validation for CE-System Incubation of Phe-Asp-Gly 2 at p Incubation of Phe-Asp-Gly 2 at p Incubation of Gly-Asp-Phe 2 at p Incubation of Gly-Asp-Phe 2 at p Incubation of Phe-Asu-Gly at p List of publications 88
6 Abbreviations III Abbreviations AA AC API 3-AP Asn Asp Asu BGE Boc Bzl CD CE CM-β-CD CPA CZE DCM DIPEA DMF DA EF ESI FAB Fmoc GC Gly BTU is P-β-CD PLC ID LD LQ M-β-CD MS MS/MS D amino acid acetonitrile atmospheric pressure ionization 3-aminopyridine asparagine aspartic acid aminosuccinimidyl background electrolyte tert-butyloxycarbonyl benzyl cyclodextrin capillary electrophoresis carboxymethyl-β-cylodextrin corrected peak area capillary zone electrophoresis dichlormethane diisopropylethylamine dimethylformamide desoxyribonucleic acid electroosmotic flow electrospray ionization fast atom bombardment 9-fluorenylmethoxycarbonyl gas chromatography glycine -[(1-benzotriazol-1-yl)(dimethylamino)methylene]--methylmethanaminium hexafluorophosphate -oxide histidine hydroxypropyl-β-cyclodextrin high performance liquid chromatography internal diameter limit of detection limit of quantitation methyl-β-cyclodextrin mass spectrometry tandem mass spectrometry outer diameter
7 Abbreviations IV paba pamba Phe PIMT RP RSD SD Ser SPPS S-β-CD t 1/2 tbu TFA TLC Trp UV para-aminobenzoic acid para-aminomethylbenzoic acid phenylalanine protein L-isoaspartyl--methyltransferase reversed phase relative standard deviation standard deviation serine solid phase peptide synthesis sulphated β-cyclodextrin half-life tert-butylester trifluoroacetic acid thin layer chromatography tryptophane ultraviolet
8 1. Introduction 1 1. Introduction 1.1 Instability of aspartyl peptides Peptides and proteins are used as pharmaceuticals for many indications and as diagnostics. Advantages of this class of substances are the high activity and high specificity, which correlates with relatively low systemic toxicity. The development of recombinant DA technology allowed the industrial production of these substances and dramatically increased the number of peptides and proteins on the pharmaceutical market. During the years , a total of 64 biopharmaceuticals was approved for human use in orth America and Europe, all of them being protein-based drugs . This represents over a quarter of all new drug approvals of the same period of time. Moreover, in 2003 approximately further 500 candidate biopharmaceutical substances were undergoing clinical evaluation . f the proteins thus far approved within the European Union, hormones and cytokines represent the largest product categories, additional classes are recombinant blood coagulation factors, subunit vaccines and monoclonal antibodies . In addition, many medical agents are synthetic peptides and peptidomimetics. Degradation pathways of peptides and proteins comprise chemical and physical instability (figure 1). Chemical instability involves covalent modifications, such as hydrolysis, deamidation, beta-elimination, oxidation, enantiomerization and disulfide exchange . In contrast, physical instability refers to changes in the higher order structure of the proteins, such as denaturation, aggregation, precipitation and adsorption. These processes can lead to loss of structure and physiological activity as well as to the formation of toxic degradation products. Physical Instability Chemical Instability Deamidation Denatured ative xidation ydrolysis Adsorption Aggregation Racemization Isomerization Beta-Elimination Precipitation Disulfide Exchange Figure 1: Degradation pathways of peptides and proteins
9 1. Introduction 2 R R R L-Asp peptide L-Asu peptide β-l-asp peptide R R R D-Asp peptide D-Asu peptide β-d-asp peptide Figure 2: Spontaneous isomerization and enantiomerization of L-Asp in peptides. Asparagine (Asn) and aspartic acid (Asp) are among the most unstable amino acids in peptides and proteins. Both of them are susceptible to isomerization and enantiomerization and Asn can additionally undergo side chain deamidation [3-5]. The initial event in these nonenzymatic reactions is the formation of an aminosuccinimidyl (Asu) intermediate (figure 2). The mechanism of succinimide formation involves deprotonation of the carboxyl-side backbone amide followed by attack of the anionic nitrogen on the side chain carbonyl group. The rate of the succinimide formation depends on the primary sequence of the peptide as well as temperature, p, concentration and ionic strength of the solution [3-8]. eighbouring amino acid residues that allow for chain flexibility and hydrogenbonding interactions such as Gly or Ser facilitate the formation of the succinimidyl intermediate. The succinimide is subject to spontaneous hydrolysis generating either the native L-aspartyl residue (α-asp) or L-isoaspartyl residue (iso-asp or β-asp), in which the peptide backbone chain is connected via the β-carboxyl group of the Asp side chain (figure 2). In addition, enantiomerization of the L-succinimide to D-succinimide may occur, due to the increased acidity of the succinimidyl α-carbon compared to Asp . It has been demonstrated that the rate of Asp enantiomerization in peptides is about 10 5 times faster than that of Asp itself under similar conditions [9, 10]. Successive hydrolysis of the D-succinimide leads to the formation of D-Asp and D-iso-Asp (D-β-Asp) residues (figure 2). Beside being side reactions in solution and solid phase peptide synthesis [11, 12], deamidation, isomerization and enantiomerization of Asn and Asp are degradation reactions of natural proteins and of peptide and protein drugs [3, 4]. Spontaneous deamidation and isomerization of Asn were found in prion proteins . abuchi and coworkers studied the stability of human parathyroid hormone under acidic and alkaline conditions and found deamidation and isomerization of Asn residues after incubation at p 9 . Fibrillar deposits of β-amyloid proteins containing β-asp occur in Alzheimer s
10 1. Introduction 3 disease [15, 16]. Succinimidyl peptides were found in heat stressed solutions of the human amylin synthetic analog pramlintide . Enantiomerization and formation of D-Asp peptides could be detected by RP-PLC after incubation of the peptidomimetic klerval . Age-dependent accumulation of D-Asp was observed in numerous human tissues, such as tooth dentine, skin, bones and ocular lens  and has been used to date paleontological material . To minimize the accumulation of damaged aspartyl residues in cellular proteins, all mammalians tissues possess the protein L-isoaspartyl--methyl-transferase (PIMT) . This enzyme uses S-adenosyl-L-methionine to methylate L-β-Asp residues but not normal L-α-Asp residues. on-enzymatic deesterification of the methylated residues returns them to the succinimide form much more rapidly than in the absence of methylation, resulting in the eventual conversion of most of the damaged residues to the L-Asp form. owever, this protection reaction is less efficient in presence of D-β-Asp residues causing accumulation of this form of the amino acid. In addition, proteins containing D-amino acids can normally not be metabolized and β-asp proteins have been found to be immunogenic . Thus, it is clear that degradation reactions of Asp play a major role in the instability of natural proteins as well as peptide and protein pharmaceuticals. They are relatively rapid reactions and were observed in a number of compounds. Any of these reactions can occur during production, isolation, purification, delivery and storage of peptide and protein drugs. The investigation of the mechanism of these degradation reactions is important for understanding the pathogenesis of some diseases as well as for optimizing processing and formulation conditions of proteins drugs. 1.2 Analytical techniques to investigate aspartic acid degradation Chemical instability of peptides and proteins has been investigated by different analytical techniques. Changes in mass, size, charge, hydrophobicity as well as in UV absorption and fluorescence are used to monitor the degradation reactions . Replacement of an amide by a carboxylic acid as consequence of deamidation causes changes in hydrophobicity, polarity and charge. Such changes can be studied by chromatographic methods, such as ion-exchange chromatography, reversed phase high performance liquid chromatography (RP- PLC), hydrophobic and affinity chromatography as well as by electrophoretic techniques, such as isoelectrofocusing, gel electrophoresis and capillary electrophoresis (CE) [4, 22]. Moreover, the mass change ( m = +1 m/z) can be detected by mass spectrometry. Isomerization of Asp to β-asp can be observed through blockage of Edman sequencing, because the extra carbon in the backbone of the β-asp residue prevents cyclization to form the anilinothiazolone derivative . A direct assay for β-asp residues involves the use of PIMT for selectively labeling β-asp sites with a 3 - or 14 C-methyl group . Tritiated methanol is released from the methylated intermediate and can be detected by scintillation counting. To obviate the need for radioactive
11 1. Introduction 4 materials, an alternative method using PIMT analyzes S-adenosyl-L-homocystein, which like methanol is produced in stoichiometric amounts by PIMT-dependent methylation of β-asp residues. UV detection is carried out after separation by RP-PLC or strong cation-exchange PLC [24-26]. Recently Cloos and Fledelius  developed immunoassays to determine the level of Asp isomerization in collagen. Enantiomerization of amino acids in peptides was first analyzed after hydrolysis of the peptide in 6 M Cl at 100 C for 6h . Aspartic acid was separated from the other amino acids by anion exchange chromatography and was analyzed by chiral gas chromatography (GC) or by RP-PLC after derivatisation to give diastereoisomers. This method is time-consuming and, because of the acidcatalyzed enantiomerization occurring during hydrolysis [29-31], does not provide reproducible results. Moreover, the presence of α- and β-asp linkages in the peptide can not be distinguished by the amino acids analysis and has to be established indirectly through the β-asp resistance to Edman degradation. Enantiomerization of an amino acid in peptides results in the formation of diastereomers which can be distinguished from one other based on differences in physico-chemical properties. Differences in hydrophobicity and polarity can be exploited relatively easily by RP-PLC. Because RP-PLC is a well-established technique, most of the studies on the degradation of Asp were conducted with this method [6, 8, 10, 32]. 1.3 Aim of the work While many studies were published on the factors that influence the formation of the succinimidyl intermediate and the isomerization of Asp [3-8, 33], only few investigated Asp enantiomerization in peptides. Geiger and Clarke demonstrated extensive enantiomerization of Asp in model hexapeptides . liyai and Borchardt detected in p 10 incubations of Asp hexapeptides additional peaks by RP- PLC that were assigned as the corresponding D-Asp peptides based on the retention time in relation to a standard and by off-line fast atom bombardment mass spectrometry as well as enzymatic cleavage studies . D-Asp peptides could be detected by RP-PLC after incubation of the peptidomimetics klerval  and of human αa-crystallin . To date only the work of Geiger and Clarke reported on the half-life (t 1/2 ) of the hydrolysis and enantiomerization of a model hexapeptide , but the kinetic of the degradation reaction has not been extensively investigated. Moreover, no report on the kinetic of the succinimidyl intermediate has been published. Most of the studies on Asp degradation were conducted by RP-PLC with UV detection, while CE was not tested as analytical method. The present study was conducted in order to investigate the degradation products arising from the degradation of aspartyl peptides in acidic and alkaline media, specifically with respect to the isomerization and enantiomerization of the Asp residue. The tripeptides Phe-Asp-Gly 2 and Gly-Asp-Phe 2 were used as model compounds in order to compare the influence of the steric
12 1. Introduction 5 hindrance of the Asp-following amino acid on the degradation. For this purpose, the following points were evaluated: 1. Synthesis of α/β-d/l-aspartyl peptides as reference substances 2. Identification of the degradation products arising after incubation of the model tripeptides 3. Development and validation of an analytical method for the separation of the degradation products 4. Quantification of the degradation products and investigation of the kinetics of the degradation reactions 5. Investigation of the degradation of a succinimidyl model peptide.
13 2. Synthesis of reference substances 6 2. Synthesis of reference substances In order to investigate the degradation of the model aspartyl peptides Phe-Asp-Gly 2 and Gly-Asp- Phe 2, pure degradation products were required as reference substances. When available, amino acids and dipeptides were purchased from commercial sources. Tripeptides with amidated C-terminal end were provided by Dr. Sabbah and were synthesised according to . Tripeptides with a free terminal carboxylic function were synthesised by manual solid phase peptide synthesis (SPPS) according to standard procedures . 2.1 Solid phase peptide synthesis of tripeptides with free terminal carboxyl group In SPPS a polymer matrix serves simultaneously as solid support for the peptide and as permanent C-terminal protecting group. Synthesis starts with the attachment of the first α -protected amino acid to the linker functionality of the resin. After deprotection of the amino function another activated protected amino acid is added. Chain elongation is accomplished by consecutive deprotection and coupling steps. Finally, the -terminal free peptide is cleaved from the resin with concomitant deprotection of all side chain functionalities. Advantages of SPPS compared to solution synthesis are: (I) the easy and fast washing and filtration steps, (II) the possibility of using reagents in excess thus achieving practically quantitative reactions, (III) the minimization of physical losses as the peptide remains attached to the support throughout the synthesis and (IV) the possibility of automation. The two most extensively used protecting combinations in SPPS are the Merrifield strategy (Boc/Bzlchemistry) and the Sheppard strategy (Fmoc/tBu-chemistry). In the Merrifield system tertbutyloxycarbonyl (Boc) is used as α -protecting group. This can be quantitatively removed using 20-50% trifluoroacetic acid (TFA) in dichloromethane (DCM). Benzyl esther derived (Bzl) side-chain protecting groups as well as the C-terminal linker to the solid support are removed simultaneously employing acids like hydrofluoric acid. ther than in the classical Merrifield system, the Sheppard strategy uses 9-fluorenylmethoxycarbonyl (Fmoc) as α -protecting group. This group can be rapidly and quantitatively removed in alkaline medium in presence of piperidine. Permanent protection of side-chain groups is provided by tert-butyl derivatives (tbu), which can be cleaved in acidic medium under relatively moderate conditions such as 95% TFA in DCM. Because of its milder reaction conditions, the Fmoc strategy is nowadays well established as method of choice in SPPS. Tripeptides with free terminal carboxylic function were synthesised by Fmoc-based SPPS. A reaction scheme is depicted in figure 3. Polystyrene with a 2-chlorotrityl chloride linker was used as resin. The Fmoc-protecting group was removed treating the peptidyl-resin with piperidine in dimethylformamide (DMF). Amide bond formation involved in situ chemical activation of the carboxy group by -[(1-
14 2. Synthesis of reference substances 7 R Cl Piperidine/DMF Repeat 2 R Cl Fmoc-AA/BTU/DIPEA R R Cl i) Piperidine/DMF ii) TFA/ 2 2 R R Cl Figure 3: Fmoc-based solid phase peptide synthesis. benzotriazol-1-yl)(dimethylamino)methylene]--methylmethanaminium hexafluorophosphate -oxide (BTU). Cleavage of the products from the resin was achieved with 95% TFA in water. The crude peptides were purified by preparative PLC and their identity was confirmed by mass spectrometry. 2.2 Synthesis of succinimidyl peptides Formation of succinimidyl derivatives is a well known side reaction during both in solution and solid phase synthesis of aspartyl peptides [35-37]. Schön and Kisfaludy  investigated the formation of Asu peptides during removal of the tbu aspartyl side-chain protecting group under different reaction conditions. While no cyclization occurred in TFA, over 95% transformation of the protected peptide to the Asu peptide was estimated by thin layer chromatography (TLC) after treatment with 3.6 Cl in acetic acid. A scheme of the reaction is depicted in Figure 4. This reaction was used to synthesise the succinimidyl peptides Phe-Asu-Gly 2, Phe-Asu-Gly, Gly-Asu-Phe 2 and Gly-Asu-Phe by
15 2. Synthesis of reference substances 8 R R Cl/Ac 15 min R R C 3 3 C C 3 R R Cl/Ac 5 days R R Figure 4: Synthesis of succinimidyl peptides according to . prolonged incubation of the corresponding aspartyl peptides in Cl/acetic acid. The peptides were purified by preparative PLC and their identity was confirmed by mass spectrometry.
16 3. Incubation of model peptides 9 3. Incubation of model peptides 3.1 Incubation of Phe-Asp-Gly 2 and Gly-Asp-Phe 2 The purpose of this work was to study the degradation of aspartic acid in peptides with special regard to the enantiomerization reaction. The model tripeptides Phe-Asp-Gly 2 and Gly-Asp-Phe 2 were selected in order to compare the influence of steric hindrance of the Asp-following amino acid because the initial succinimide formation resulting in the corresponding Asu peptides is known to depend on the amino acid sequence [7, 39, 40]. As it has been demonstrated that different degradation products are formed depending on the p of the solution [6, 8, 14], the aspartyl peptides were incubated at two different p values (p 2 and 10). In addition to isomerization and enantiomerization, further degradation reactions can be expected [3, 5]. Deamidation of the C-terminal amide and hydrolysis of the peptide amide backbone can occur. A diketopiperazine derivative can be the result of the nucleophilic attack of the -terminal nitrogen on the amide carbonyl between the second and the third amino acid [41, 42]. Previous studies have shown that hydrolysis of the peptide backbone is predominant at acidic p while isomerization occurs in alkaline media [6, 14]. Moreover, the succinimide is relatively stable at low p while it is rapidly hydrolysed under neutral and alkaline conditions [43, 44]. As the enantiomerization of Asp occurs rather slowly, the incubation was performed at 80 C, in order to accelerate the reaction rate. 3.2 Incubation of Phe-Asu-Gly After analysis of the incubations of Phe-Asp-Gly 2 and Gly-Asp-Phe 2 it was clear that various reactions contribute to the degradation of the Asp peptides (see chapter 4). In order to investigate the enantiomerization reaction independently from other degradation pathways, the model compound Phe-Asu-Gly was incubated. In this way, the hydrolysis reaction of the Asu ring and the consequent formation of α- and β- D/L-Asp products could be selectively studied. After analysis of the incubation of Phe-Asp-Gly 2 it was shown that no ring opening occurs at p 2 (see paragraph 4.2). For this reason, the Asu peptide was incubated only in alkaline medium (p 10). A preliminary experiment was conducted incubating Phe-Asu-Gly at a temperature of 80 C. Under these conditions the succinimidyl hydrolysis proceeds too fast to allow detection of the degradation of Phe-Asu-Gly in reasonable time intervals. An incubation temperature of 30 C proved to be convenient for the analysis.
17 4. Analysis of degradation products by CE Analysis of degradation products by capillary electrophoresis 4.1 Basic principles of peptide analysis by CE CE is one of the most important techniques for the separation of charged substances. In capillary zone electrophoresis (CZE) analytes are separated due to their different mobility in a fused-silica capillary filled with an electrolyte solution when an electric field is applied. Equation (1) describes the mobility µ of a compound in terms of the physical parameters q (ion charge), η (solution viscosity) and r (ion radius): µ = q 6 π η r (1) From this equation it is evident that small, highly charged species have high mobilities whereas large, minimally charged species have low mobilities. Electrophoretic mobility depends on: (I) properties of the analyte itself such as charge, size, shape, (II) properties of the background electrolyte (BGE) such as composition, p, ionic strength, viscosity, temperature and (III) interactions between analytes and components of the BGE such as solvatation, dissociation and complex formation. Another parameter that influences the migration time is the electroosmotic flow (EF). The EF is the bulk flow of liquid in the capillary as a consequence of the surface charge on the interior capillary wall. The EF results from the effect of the applied electric field on the solution double-layer at the wall. Changes in the chemistry of the capillary wall can affect the velocity of the EF profoundly and subsequently the migration time. Moreover, the EF causes movement of nearly all species, regardless of charge, in the same direction. Thus, cations, neutral species and anions can be detected in a single run since they all migrate in one direction. Differences in the primary structure of peptides cause differences in their properties such as electric charge, size, shape and hydrophobicity. Because of its ability to exploit these physico-chemicals properties, CE is a convenient, fast and simple analytical technique for the separation of peptides. In the last years many application of CE for the separation of peptides have been demonstrated [45-47]. In a previous study a CE and a RP-PLC method for the analysis of Asn peptides were compared. CE showed better resolution in shorter analysis time . Moreover, it has been shown that CE can separate Asp and β-asp peptides and the corresponding D-Asp and β-d-asp diastereomers [49-51]. This is due to small differences of the pk a values of both the α-asp and β-asp isomers as well as the respective diastereomers.
18 4. Analysis of degradation products by CE Analysis by CE in achiral buffer Analysis of the incubation solutions was first performed by CE with UV detection. An aqueous phosphate buffer, p 3.0, was used as background electrolyte (BGE). Using reference substances it has been previously shown that the diastereomeric pairs L-Phe-α-L-Asp-Gly 2 /L-Phe-α-D-Asp- Gly 2 and L-Phe-β-L-Asp-Gly 2 /L-Phe-β-D-Asp-Gly 2 can be separated under these conditions . Identification of the degradation products was carried out by co-injection of reference substances. Potential degradation products that were available as reference substances were injected. Most of the degradation products could be identified in this way. Unequivocal identification was performed by online mass spectrometry (MS) detection (see chapter 6). By MS, identification of peaks that were previously not assigned by co-injection was achieved. Subsequently, missing reference substances were synthesised and co-injected. As it can be seen in Figure 5, a phosphate buffer, p 3.0 (CE-System 1, see Materials and Methods, paragraph 10.4), was suitable for the analysis of most degradation products in the presence of p-aminomethylbenzoic acid (pamba) as internal standard. Most of the expected degradation products could be identified in the solutions after incubation at p 2 (see Figures 5A and 5C). Deamidation of the C-terminal amide resulted in the free acid form of the tripeptides. The dipeptides Phe-Asp and Gly-Asp and the amino acid amides Phe 2 and Gly 2 were the result of hydrolysis of the peptide amide backbone. The Asu peptides could be detected due to their relative stability in acidic solution. Peptides containing D-Asp were not observed as the acidity of the succinimidyl α-carbon is not sufficiently high to be deprotonated at p 2, so that no significant epimerization occurred. As expected, incubations at p 10 resulted in different degradation patterns compared to p 2 (see Figure 5B and 5D). Cleavage of the backbone amide was not significant while isomerization and enantiomerization via the cyclic imide dominated. Deamidation of the C-terminal amide to yield the corresponding free acids was observed for all isomers of the tripeptides. The intermediate Asupeptides were not detected, due to their low stability under these incubation conditions. As predicted from the pk a values for the α- and β-asp free carboxyl groups, the β-asp peptides showed longer migration times than the corresponding α-asp peptides. In fact, studies on glycin-asp peptides showed that the α-asp side chain carboxyl group is less acidic than the α-carboxyl group of β-asp, having pk a values of 4.0 and 3.4, respectively [43, 44]. Interestingly, the succinimidyl peptides Phe-Asu-Gly and Gly-Asu-Phe migrated slower than the corresponding aspartyl peptides Phe-Asp-Gly and Gly-Asp-Phe, despite the fact that the Asp peptides possess two carboxyl groups and a higher molecular mass compared to the Asu peptides. This may be explained by an increased acidity of the C-terminal carboxyl group due to succinimide formation, so that the succinimidyl peptides carry a lower overall positive charge than the Asp peptides. Alternatively, this behaviour may be attributed to different hydrodynamic volumes of the compounds.
19 4. Analysis of degradation products by CE 12 A: Phe-Asp-Gly 2 p 2 B: Phe-Asp-Gly 2 p 10 Gly 2 pamba Phe-Asu-Gly 2 L-Phe-α-L-Asp-Gly 2 L-Phe-α-L-Asp-Gly Phe-Asp Phe-Asu-Gly Phe pamba L-Phe-α-D-Asp-Gly 2 L-Phe-α-L-Asp-Gly 2 L-Phe-α-L/D-Asp-Gly L-Phe-β-L-Asp-Gly 2 L-Phe-β-D-Asp-Gly 2 L-Phe-β-L-Asp-Gly L-Phe-β-D-Asp-Gly Time (min) Time (min) C: Gly-Asp-Phe 2 p 2 D: Gly-Asp-Phe 2 p 10 Phe 2 Gly-Asu-Phe 2 pamba Gly-α-L-Asp-L-Phe 2 Gly-Asp Gly-α-L-Asp-L-Phe Gly-Asu-Phe Phe pamba Gly-α-L/D-Asp-L-Phe 2 Gly-β-L-Asp-L-Phe 2 Gly-β-D-Asp-L-Phe 2 Gly-α-L-Asp-L-Phe Gly-α-D-Asp-L-Phe Gly-β-L-Asp-L-Phe Gly-β-D-Asp-L-Phe Time (min) Time (min) Figure 5: Electropherograms of Phe-Asp-Gly 2 incubated at p 2 for 12 h (A) and at p 10 for 24 h (B) and Gly-Asp-Phe 2 incubated at p 2 for 36 h (C) and at p 10 for 96 h (D). Analysis conditions: 50 mm phosphate buffer, p 3.0. For details see Materials and Method, CE-System 1, paragraph Analysis by CE using cyclodextrins Under the conditions used in CE-system 1 (phosphate buffer, p 3.0), resolution of all analytes could not be achieved. As it can be seen in Figures 5D and 5B, the diastereomeric pair Gly-α-L-Asp-L- Phe 2 and Gly-α-D-Asp-L-Phe 2 was not resolved and only partial separation of L-Phe-β-L-Asp- Gly 2, L-Phe-α-L-Asp-Gly and L-Phe-α-D-Asp-Gly was possible. An additional selectivity can be reached by addition of a chiral compound into the BGE. Cyclodextrins (CDs) are the most popular chiral selectors for peptide enantioseparations in CE [52, 53]. CDs are dissolved in the BGE and form with analytes host-guest inclusion complexes. These transient diastereomeric complexes differ in their physico-chemical properties and can thus be separated. Similarly to enantiomers, diastereomers can
20 4. Analysis of degradation products by CE 13 form complexes with CDs which have different properties and thus electrophoretic mobilities. Consequently, separations that can not be achieved in an achiral medium can be often observed in the presence of CDs. Resolution of Gly-α-L/D-Asp-L-Phe 2 and of L-Phe-β-L-Asp-Gly 2 and L-Phe-α-L/D-Asp-Gly was attempted by addition of cyclodextrins to the BGE. Various uncharged and negatively charged CDs such as β-cd, methyl-β-cd (M-β-CD), carboxymethyl-β-cd (CM-β-CD), hydroxypropyl-β-cd (P-β-CD) and sulfated-β-cd (S-β-CD) were tested. Concentrations of the selector, p of the BGE and applied voltage were optimized for every sample by injection of reference substances Analysis of Gly-α-L-Asp-L-Phe 2 and Gly-α-D-Asp-L-Phe 2 Figure 6 A shows the separation of the diastereomeric pair Gly-α-L-Asp-L-Phe 2 and Gly-α-D-Asp- L-Phe 2. Base-line resolution was obtained by the addition of 3 mg/ml S-β-CD to 50 mm phosphate buffer, p 3.0 (CE-System 2, Materials and Methods, paragraph 10.4). The DL diastereomer migrates faster than the LL diastereomer which can be of advantage because of the low concentration of the DL diastereomer in the incubation solutions. In fact, it is always desirable that the minor substance migrates ahead of the principal component, in order to avoid the minor peak to be obscured by eventual tailing of the major peak. A negative consequence of the use of CDs is that the internal standard pamba can not be detected. This is probably due to the strong interaction between CDs and pamba. egatively charged CDs, such as S-β-CD, migrate towards the anode. Substances which interact strongly with the chiral selector form negatively charged complexes which migrate towards the anode as well. Because analyte detection is carried out at the cathodic end of the capillary, these negatively charged complexes can not reach the detection window and can consequently not be detected. Assuming that the UV-absorption properties of the diastereomers are identical, the total concentration of Gly-α-L- Asp-L-Phe 2 and Gly-α-D-Asp-L-Phe 2 were calculated from the area of the peak corresponding to both peptides in the analysis in the achiral BGE (CE-system 1). After separation with CDs (CEsystem 2) the peak-area ratio of the two analytes and consequently the amount of Gly-α-L-Asp-L- Phe 2 and Gly-α-D-Asp-L-Phe 2 were determined Analysis of L-Phe-β-L-Asp-Gly 2, L-Phe-α-L-Asp-Gly and L-Phe-α-D-Asp-Gly The best separation of L-Phe-β-L-Asp-Gly 2, L-Phe-α-L-Asp-Gly and L-Phe-α-D-Asp-Gly was achieved by the addition of CM-β-CD to the phosphate buffer. As already discussed for the use of S-β-CD, the internal standard was not detected under these conditions. While L-Phe-α-L-Asp-Gly
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EXPERIMENT 5: DIPEPTIDE RESEARCH PROJECT Pre-Lab Questions: None. 64 I. Background Information DIPEPTIDE RESEARCH PROJECT Methods developed by organic chemists for the synthesis of biopolymers have had
IV. -Amino Acids: carboxyl and amino groups bonded to -Carbon A. Acid/Base properties 1. carboxyl group is proton donor! weak acid 2. amino group is proton acceptor! weak base 3. At physiological ph: H
Amino Acids, Peptides, Proteins Functions of proteins: Enzymes Transport and Storage Motion, muscle contraction Hormones Mechanical support Immune protection (Antibodies) Generate and transmit nerve impulses
Application ote A098 Small μmol Scale Synthesis of a Labeled Antimicrobial Peptide Page 1 Small μmol Scale Synthesis of a Labeled Antimicrobial Peptide using Biotage Initiator+ Alstra Introduction Labeled
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ChemActivity 46 Amino Acids, Polypeptides and Proteins 1 ChemActivity 46 Part A: Amino Acids and Peptides (Is the peptide IAG the same as the peptide GAI?) Model 1: The 20 Amino Acids at Biological p See
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an ABC Laboratories white paper Correlation of the Mass Spectrometric Analysis of Heat-Treated Glutaraldehyde Preparations to Their 235nm / 280 nm UV Absorbance Ratio A. Sen, R. Dunphy, L. Rosik Analytical
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Peptides: Synthesis and Biological Interest Therapeutic Agents Therapeutic peptides approved by the FDA (2009-2011) 3 Proteins Biopolymers of α-amino acids. Amino acids are joined by peptide bond. They
SPE and HPLC Dr Iva Chianella Lecturer in Analytical Chemistry Cranfield Health +44 (0) 1234 758322 email@example.com Solid-Phase Extraction- SPE Simple, fast and efficient sample preparation
Application Notes # LCMS-35 esquire series Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water An LC-APCI-MS/MS method using an ion trap system
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Monoclonal ntibody Fragment Separation and haracterization Using Size Exclusion hromatography oupled with Mass Spectrometry uthors Haiying hen Katherine McLaughlin Sepax Technologies, Inc. 5 Innovation
Combinatorial Chemistry and solid phase synthesis seminar and laboratory course Topic 1: Principles of combinatorial chemistry 1. Introduction: Why Combinatorial Chemistry? Until recently, a common drug
Insulin Lispro C 257 H 383 N 65 O 77 S 6 Mol. Wt. 5808 28 B -L-Lysine-29 B -L-proline insulin (human) Insulin Lispro is a 2-chain peptide containing 51 amino acids. The A- chain is composed of 21 amino
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Peptide Library Synthesis Jamie M. R. Moore Guy Laboratory UCSF I. verview.. page 2 II. Reagents and Apparatus. page 4 III. Flow Chart. page 6 IV. Protocol. page 7 IV. Tables A. List of Fmoc Amino. page
ORIGINAL SCIENTIFIC PAPER 269 Determination of the Enantiomers of Methionine and Cyst(e)ine in the Form of Methionine-sulphon and Cysteic Acid After Performic Acid Oxidation by Reversed Phase High Performance
The Essential CHROMacademy Guide Mobile Phase Optimization Strategies for Reversed Phase HPLC Webcast Notes Type your questions in the Submit Question box, located below the slide window You can enlarge
52 May/June 2012 Intelligent use of Relative Response Factors in Gas Chromatography-Flame Ionisation Detection by Karen Rome and Allyson McIntyre, AstraZeneca, Macclesfield, SK10 2NA, UK Quantitative analysis
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CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY 9.1 POLICY This test method may be used to confirm the presence of zolpidem (ZOL), with diazepam-d 5 (DZP-d 5 ) internal standard, in
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Purification of reaction mixtures using flash chromatography. This technical note details the use of ISOLUTE Flash chromatography columns for the purification of reaction mixtures. What is flash chromatography?
Aspects of industrial purification of peptides using large-scale chromatography Introduction By Lars Andersson and Jonas Persson PolyPeptide Laboratories (Sweden) AB PO Box 30089 SE-200 61 LIMHAMN SWEDEN
Protein physics, Lecture 5 Peptide bonds: resonance structure Properties of proteins: Peptide bonds and side chains Proteins are linear polymers However, the peptide binds and side chains restrict conformational
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Isomers ave same molecular formula, but different structures Constitutional Isomers Differ in the order of attachment of atoms (different bond connectivity) Stereoisomers Atoms are connected in the same
TE EMIAL SYTESIS F PEPTIDES Peptides are the long molecular chains that make up proteins. Synthetic peptides are used either as drugs (as they are biologically active) or in the diagnosis of disease. Peptides
Guidance for Industry Q2B Validation of Analytical Procedures: Methodology November 1996 ICH Guidance for Industry Q2B Validation of Analytical Procedures: Methodology Additional copies are available from:
A ovel Approach to Quantify Unbound Cisplatin, Carboplatin, and xaliplatin in Human Plasma Ultrafiltrate by Measuring Platinum-DDTC Complex Using LC/M/M Min Meng, Ryan Kuntz, Al Fontanet, and Patrick K.
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Application Note Determination of Amino acids by UHPLC with automated PA- Derivatization by the Autosampler Category Bio Analysis Matrix - Method UHPLC Keywords Proteinogenic Amino acids, Canonical Amino
October 2006 RESTRICTED LUMEFANTRINE Draft proposal for The International Pharmacopoeia (October 2006) DRAFT FOR DISCUSSION World Health Organization 2006 All rights reserved. This draft is intended for
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CHEM CHEMISTRY Note: See beginning of Section F for abbreviations, course numbers and coding. CHEM 1041 General Chemistry I 3 ch (3C 1T) Introductory course designed primarily for B.Sc. students. Topics
Peptide Bond Peptide Bond Amino acids are linked together by peptide bonds to form polypepetide chain. + H 2 O 2 Peptide bonds are strong and not broken by conditions that denature proteins, such as heating.
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Analysis of proteins Western blot Protein seperation (liqiuid chromatography) Mass spectrometry Assaying of protein in... Blood (e.g. viral infections, pregnancy test) Cells Tissue Urin (bladder infection)
KMS-Specialist & Customized Biosimilar Service 1. Polyclonal Antibody Development Service KMS offering a variety of Polyclonal Antibody Services to fit your research and production needs. we develop polyclonal
CONTENTS I. INTRODUCTION II. SAMPLE PRE-TREATMENT a. Biological Samples b. Solid Samples: Soil, Whole Foods, Tissue c. Aqueous Samples: Water, Beverages d. Non-Aqueous Liquid III. SOLID PHASE EXTRACTION
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