Quality. Now Certified to ISO 9001:2008

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1 Quality Now Certified to ISO Quality Policy It is Peptides International's goal is to achieve complete customer satisfaction by addressing customer needs and delivering what we promise. The company is committed to the Quality Management System and through review of quality objectives and metrics, is working towards its improvement and effectiveness. Specifications and Purity Assays Each product is accompanied by a detailed lot-specific analytical data sheet containing all specifications. In addition to specification data, additional test results can be made available upon customer request. Product integrity is assured using techniques that include thin layer chromatography, high performance liquid chromatography, optical rotation, nuclear magnetic resonance, IR spectroscopy, and mass spectrometry. Peptide Institute Products from the Peptide Institute of Osaka, Japan are manufactured under strict quality control standards and are accompanied by analytical data sheets directly from the Institute. Peptide International products are designated by a -PI suffix to distinguish them from those produced by the Peptide Institute. x Order Hotline

2 Net Peptide Weight Some products from the Peptide Institute are distributed in premeasured vials. These are indicated by the suffix -s, or -v in their catalog number. The net peptide weight is precisely determined by amino acid analysis after acid hydrolysis, HPLC, and/or UV absorption, and the value is indicated clearly on the vial label. The indicated weight is only for the net peptide molecule, and the weights of any constituents (water or acetic acid) are excluded from the quantity. The amount of usable peptide meets and may exceed its advertised quantity. Stability and Storage Complex peptides and antisera should generally be kept dry and frozen to prevent unnecessary deterioration, although they are stable at ambient temperature for several days. Almost all biologically active peptides are readily soluble in water or any suitable buffer solution. Stability of peptides in solution is relatively low even below -20 C. Therefore a peptide solution, once prepared, should be used as soon as possible. If the solution must be stored overnight, it should be kept in a freezer at temperatures lower than -20 C. When prolonged storage has occured, homogeneity of the peptide in the solution should be reconfirmed prior to use. Please refer to the following table and to our website for additional information regarding stability and storage conditions. Order Hotline xi

3 Product Storage Conditions Product Series Number Storage Condition L- & D-Amino Acids 2700 & 2800 RT Boc-Amino Acids 2000 & C Fmoc-Amino Acids 1700, 1800, & C Unusual Amino Acids C Reagents 1000 Varies RT, 4 C, or -20 C Unsubstituted Resins 1000 & 9000 RT Substituted Resins 1000, 10000, 1100, 11000, 4 C 1200, 12000, 1300, 1400, & 9000 All CLEAR Resins 1200, C Peptides 3000 & C Inhibitors 3000 & C Substrates C Antisera 8000, 80000, & C Carbohydrates C RT =Room Temperature4 4 C = Refrigerator -20 C = Freezer All products (except RT) are stored in freezers for inventory purposes. xii Order Hotline

4 All of the products listed in this catalog are rigorously tested and the purity of the products is guaranteed according to the following criteria 1000 Series These reagent grade chemicals are of a quality confirmed by our chemists in Japan and the USA to be acceptable for peptide synthesis. 1100, 1200, 1300,1400, 10000, and Series These substituted resins are manufactured in our Louisville, Kentucky facility. The parameters, described on the analytical data sheet included with each resin, are substitution, percent racemization (where appropriate), bead size, and percent cross-linking Series Fmoc-Amino Acids of the 1700 Series No free amino acid is detectable by TLC when 100 micrograms is applied to the plate. Purity determined by HPLC analysis is higher than 98% Series Amino acids of the 2000 Series The homogeneity of the amino acids is monitored by thin layer chromatography (TLC) and is guaranteed to yield a single spot when 50 micrograms is applied to the plate. The optical purity of amino acids is higher than 99.9% except in the case of serine, which is frequently contaminated by more than 1% enantiomeric serine; data are available on request. Optical purity is determined by Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) using chiral derivatizing reagents. Order Hotline xiii

5 Amino Acid Esters and Side Chain Derivatives of the 2000 Series The following letters are used to designate the different grades AA A single spot is always detectable by two different TLC systems when 200 micrograms is applied to the plate. Homogeneity higher than 99% as determined by paper electrophoresis is guaranteed. A A trace of impurity might be detectable by TLC, but the maximum range is less than 2%. B Contamination of less than 3% might be detectable. Boc- and Z-Amino Acids of the 2000 Series The optical purity of the component amino acids is always higher than 99.9%, and contamination by the sec-butyl group is guaranteed to be less than 0.05%. The following letters are used to designate the different grades AA A single spot is detectable by TLC when 200 micrograms is applied to the plate. The amount of contamination detectable by HPLC, if any, is less than 0.5%. A A trace of impurity might be detectable by TLC, but the maximum range determined by HPLC is less than 1%. B Contamination of less than 2% might be detectable by HPLC. AA-A This compound is synthesized as material of grade AA, but may gradually decompose to grade A within one year, even under cold storage conditions. A-B This compound is synthesized as material of grade A, but may gradually decompose to grade B within one year, even under cold storage conditions. xiv Order Hotline

6 3000 Series Amino Acids and Peptide Derivatives of the 3000 Series The following letters are used to designate the different grades AA A single spot is detectable by TLC when 100 micrograms is applied to the plate. The amount of contamination detectable by HPLC is less than 1%. A A trace of impurity might be detectable by TLC when 100 micrograms is applied to the plate. The amount of contamination detectable by HPLC is less than 2%. B Contamination of less than 4% might be detectable by HPLC. A-B This compound is synthesized as material of grade A, but may gradually decompose to grade B within one year, even under cold storage conditions Series Biologically Active Peptides of the 4000 Series All of these peptides are chemically synthesized using carefully selected amino acid derivatives from our products, and strictly purified to come up to our quality standards. Impurities detectable by ordinary gradient HPLC systems are less than 1% and a single TLC spot upon application of 50 micrograms is detected in almost all of our peptides, except Met-containing peptides. Some additional minor peaks on HPLC or minor spots on TLC are detectable with Met-containing peptides since they may be easily oxidized to Met-sulfoxide even during analysis. The peptides which are followed by the words Purity Information and letter symbols, such as Purity Information Q P in this catalog, can be explained as follows Order Hotline xv

7 Q P Q E Q X Q Z These compounds have some inherent instability. During storage, even under freezer conditions, a trace of impurity may become visible by TLC analysis. The amount of impurity determined by HPLC is less than 1%. These compounds have some inherent instability. Their purity is guaranteed to be higher than 97% by HPLC. The purity of these compounds is guaranteed to be higher than 97% by HPLC. The purity of these compounds is guaranteed to be higher than 95% by HPLC. 5000, 6000, and Series These unusual amino acids, amino acid derivatives, and other offerings represent an expansion of quality products from Peptides International and are synthesized under appropriately high purity criteria Series Peptide Antisera are sold in vials as amorphous powders which are obtained after lyophilization from M Phosphate Buffer (ph 7.0). The specificity and sensitivity are determined by the enzyme immunoassay technique specified; the data for each vial are given on an attached sheet. Bacterial Enzyme Inhibitors The microbial enzyme inhibitors (Microbial Products) listed in this catalog are isolated from cultured media of microorganisms. Many of these compounds are inherently unstable and thus difficult to categorize using our normal standards of purity. The homogeneity of these compounds cannot be guaranteed except for their specific inhibitory activities. Additional data for these series of compounds are available upon request. xvi Order Hotline

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