AIO Network Analytical Chemistry



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AIO Network Analytical Chemistry basic course "Molecular Spectroscopy" May 26, 28, and June 4, 5, 2009 Wageningen University, University of Utrecht, Free University Amsterdam & University of Groningen Topic The Molecular Spectroscopy course is one of the Basic Modules of the ANAC programme. It intends to provide a level of background knowledge adequate for any analytical chemist working at the PhD/AIO level. Obviously, molecular spectroscopy covers a wide range of research and applications in the analytical chemistry world. In fact, it includes a variety of rather independently operating and developing disciplines. In this course, a selection of 4 topics is made which are considered to be of major importance in analytical chemistry, now and in the future. Specialists are invited as lecturers. Course format The course is intended for students in analytical chemistry at the graduate student (AIO) level. However, scientists from other fields, industry or from other research institutes are welcome to attend as well. The various methods and techniques will be covered in overview lectures given by experts in the different fields. Handouts will be made available giving summaries of the topics discussed. On some days a laboratory tour is foreseen. The course language will be English. Dates and venue The 4-day course will be held on May 26, May 28, June 4 and June 5, 2009 at Wageningen University, Utrecht University, VU University Amsterdam and Groningen University, respectively. Lectures will be given from approx. 10:00 to 16:30 h. Detailed programmes with the exact locations and itineraries are given on pages 4-8. Programme Day 1: Tuesday May 26, Wageningen University NMR spectrometry, with emphasis on analytical aspects and use in analytical practice. Lecturer: Dr. T.A. van Beek, Dr P. de Waard (WU) Day 2: Thursday May 28, Utrecht University IR spectrometry, with emphasis on practical aspects and up-to-date applications. Lecturer: Dr. T. Visser (UU) Day 3: Thursday June 4, Free University Amsterdam Laser spectroscopy, with emphasis on fluorescence and Raman techniques. Lecturers: Dr. F. Ariese and Prof. Dr. C. Gooijer (VUA) Day 4: Friday June 5, Groningen University The course will give a general introduction into mass spectrometry, with emphasis on applications in proteomics and biochemistry. Lecturer: Prof. Dr. R. Bischoff & Dr. P. Bruins (RUG)

Examination and certificates An examination will be organised after the course in the form of take-home assignments to be completed by the participants. Two types of certificate (with or without examination) will be made available. Further information Information on ANAC and on this course can be found at http://www2.kncv.nl/ following Vakgebieden, Analytische Chemie and ANAC (AIO netwerk) links or using the direct link: http://www2.kncv.nl/analytischesectie/nl/mainindex.asp?pageid=112&color=32. For further information concerning the course content, please contact Dr. Freek Ariese, Department of Analytical Chemistry and Applied Spectroscopy, Vrije Universiteit Amsterdam; e- mail ariese@few.vu.nl; phone 020 5987524; fax 020 5987543. Registration The registration fee is: free of charge for PhD students of the ANAC groups (see table below) 200,- for other PhD students 800,- for other participants 1 and includes coffee/tea breaks and lunches. The attached registration form should be returned to the ANAC secretariat (Dr. P. Horvatovich, University of Groningen; fax 050 36333; p.l.horvatovich@rug.nl) before 15 May 2009. After registration the participants will receive detailed information on the programme and, if required, an invoice. ANAC groups University KUN RuG TUD UL UT UU UvA VUA WUR Group(s) Analytical Chemistry Analytical Biochemistry Pharmaceutical Analysis Inorganic Chemistry BioTechnology Analytical Biosciences MESA+ Research Institute Inorganic Chemistry and Catalysis Biomedical Analysis Life Science Technologies Polymer Analysis Analytical Chemistry and Applied Spectroscopy Agrotechnology and Food Sciences 1 For these participants there is also the possibility to attend single days (at 200 per day).

AIO Network Analytical Chemistry basic course "Molecular Spectroscopy" May 26, 28, and June 4, 5, 2009 Wageningen University, University of Utrecht, Free University Amsterdam & University of Groningen REGISTRATION FORM Name:... First name:... Univ./Company:... Department:... Address:....... Fax number:... E-mail address:... Position: O ANAC-AIO/OIO O AIO/OIO other groups O other Registration fee:... Examination: O Yes O No Date:... Please e-mail or fax this form to the ANAC secretariat: c/o Dr. Péter Horvatovich Analytical Biochemistry University of Groningen Antonius Deusinglaan 1, 9713 AV Groningen Tel.: 050 363 3341 Fax: 050 363 7582 e-mail: p.l.horvatovich@rug.nl

SCHEDULE DAY 1 Tuesday 26 May 2009; Wageningen University Lecturers (alphabetically): Dr. T.A. van Beek (contact person) and Dr. P. de Waard. Location: lecture hall C3, Mathematics Building (building 308), Dreijenlaan 4, Wageningen. NMR spectrometry, with emphasis on analytical aspects and use in analytical practice. 9.30-10.00: arrival and coffee. 10.00-10.45: Dr. T.A. van Beek: Introduction to NMR. 10.45-11.00: break 11.00-11.45: Dr. P. de Waard: The role of 2-dim. NMR techniques in modern structure elucidation. 11.45-12.00: break 12.00-12.45: Dr. T.A. van Beek and Dr. P. de Waard: problem solving session 1. 12.45-13.30: lunch 13.30-13.45: visit to 400 MHz NMR, recording of an NMR spectrum 13.45-14.30: Dr. P. de Waard and Dr. T.A. van Beek: problem solving session 2. 14.30-14.45: break 14.45-15.30: Dr. P. de Waard and Dr. T.A. van Beek: LC-NMR, an introduction. 15.30-15.45: tea break 15.45-16.15: visit of 600 MHz LC-NMR of Biqualys and large scale MRI (Magnetic resonance imaging) facility 16.20-16.40: evaluation, hand-out of take-home exam, end of course. During breaks, there is opportunity to ask questions to the lecturers. See route description at next page.

SCHEDULE DAY 2 Thursday 28 May 2009; University of Utrecht Lecturers: Dr. T. Visser. Location: Lecture room OC103 IR spectrometry, with emphasis on practical aspects and up-to-date applications. The course includes the following subjects: theory (vibrations, frequency & intensity, selection rules, interactions,...) instrumentation (spectrometers and accessories) sampling techniques (transmission, reflection, emission...) quantitative analysis (Beer's law, single- and multi-component,...) data manipulation (resolution enhancement, library search,...) spectrum interpretation (organic & inorganic) applications (organic/inorganic, medical, bio-organic, physical,...) future developments Course period 10.00-16.30 h. Lunch, coffee and tea will be available. Directions: Lecture room OC103 is in the Went Building of the Utrecht University (UU), Sorbonnelaan 16, 3484 CA Utrecht (De Uithof). By public transport: Utrecht central Station; bus 12 or 12S to De Uithof/UMC. Leave the bus at stop Sorbonnelaan. The Went Building is at 2 min. walk and is often called de Ponskaart. By car: enter south ring Utrecht (A27) and follow signs Universitair Medisch Centrum (UMC). Further info: http://www.uu.nl/en/whowhatwhere/pages/default.aspx

SCHEDULE Day 3 Thursday 4 June 2009, Vrije Universiteit Amsterdam Lecturers: Dr. F. Ariese and Prof. Dr. C. Gooijer, Department of Analytical Chemistry & Applied Spectroscopy/ACAS Laser Centre VU Location: Lecture room F-619 (VU Science Building 6 th floor). Laser spectroscopy, with emphasis on fluorescence and Raman techniques. 10.00 11.00 Introduction to the course; molecular spectroscopy Review of photophysical principles (fundamental aspects of light, molecular orbitals, absorption/emission processes, electronic and vibrational transitions) 11.00 11.30 Break 11.30 12.45 Lasers in analytical chemistry (fundamental aspects of lasers and dye lasers, analytical applications, related detection principles including cavity ring-down spectroscopy) 12.45 13.30 Lunch break 13.30 14.40 Fluorescence and phosphorescence (laser-induced techniques, coupling to LC/CE, saturation effects, fluorescence resonance energy transfer (FRET), highresolution fluorescence at cryogenic temperatures, single-molecule fluorescence 14.40 15.00 Break 15.00 16.00 Raman spectroscopy (resonance Raman, surface-enhanced Raman, timeresolved Raman, coupling to separation techniques) 16.00 16.30 Guided tour to the Laser Centre VU Directions: Lecture room F-619 is on the 6 th floor of the Science building (W&N) of the Vrije Universiteit (VU); De Boelelaan 1081-1087, Amsterdam. Visitors can enter the Science building from the central campus through entrance 1085. By public transport: closest trainstation is Amsterdam Zuid WTC; from there it is a 10 min walk or one stop southbound (direction Amstelveen Poortwachter) with tram 5 or 51; get off at stop De Boelelaan-VU. By car: take ring road A10 Amsterdam zuid, exit at S108 direction Amstelveen and go left underneath the university hospital. Parking is usually possible at the south side of campus (A.J. Ernststraat); a day ticket costs Euro 8,40. See also www.vu.nl/en/about-vu-amsterdam/contact-info-and-route/index.asp

SCHEDULE Day 4 Friday 5 June 2009, University of Groningen Lecturers: Prof. Dr. Rainer Bischoff (Analytical Biochemistry) and Dr. Andries P. Bruins (Mass Spectrometry Core Facility) Location: Lecture room is located at building 3215 and room 0154. The course will give a general introduction into mass spectrometry, with emphasis on applications in proteomics and biochemistry. Level: no previous knowledge about mass spectrometry is required. 10:00 11:35 P. Bruins: General introduction and fundamentals: mass analyzers, resolution, isotopes, ionization techniques. 11:35 11:45 Break 11:45 12:30 P. Bruins: LC/MS coupling, tandem mass spectrometry. 12:30 13:15 Lunch Break: cafeteria Pharmacy 13:15 15:00 R. Bischoff: Mass spectrometry of peptides and proteins: peptide mass fingerprinting, MS/MS identification of proteins (includes 10 min break at 14:00). 13:15 14:00 R. Bischoff: Mass spectrometry of peptides and proteins: peptide mass fingerprinting, MS/MS identification of proteins. Part I. 14:00 14:10 Break 14:10 15:00 R. Bischoff: Mass spectrometry of peptides and proteins: peptide mass fingerprinting, MS/MS identification of proteins. Part II. 15:00 15:30 Coffee-tea break 15:30 17:00 R. Bischoff: Posttranslational modifications, stable isotope labelling, biomarker discovery. 17:00 17:30 P. Bruins and R. Bischoff Tour of the mass spectrometry facility and the analytical biochemistry labs. Participants should print handouts, and have them at hand during the lectures. Address: Building of the Medical Faculty (UMCG), Dentistry and Farmacie, Antonius Deusinglaan 1, 9713 AV Groningen, Lecture room: Building 15, first floor, room 154 Route description: When coming by train to Groningen, please take bus 3 (Lewenborg) or bus 6 (Beijum). The bus stop is 50 meters away from our building (take first bus stop after the "Grote Markt" with the Martini Tower ). We are located in the white/grey building with blue letters on the front of the building ("Medische wetenschappen, tandheelkunde en farmacie"). When coming by car to Groningen, it is best to park your car in the parking garage UMCG Noord. Our building is next to the road to the parking garage.