CURRICULUM VITAE ET STUDIORUM Personal data Name: Riccardo Di Mambro Education 2015-Present University of Rome Sapienza, Italy. Research work as PostDoc Title of the research grant: Meccanismi molecolari di interazione tra citochinine e auxina nel meristema di Arabidopsis. 2012-2014 University of Rome Sapienza, Italy. Research work as PostDoc Title of the research grant: Identificazione di segnali molecolari che controllano il differenziamento delle cellule staminali a distanza nel meristema di Arabidopsis. 2009-2012 University of Rome Sapienza, Italy. Ph.D. Studentship in Genetics and Molecular Biology Thesis Title: Novel mechanisms controlling auxin morphogenetic gradient in the Arabidopsis root meristem. 2009 University of Rome Sapienza, Italy. M.Sc. degree in Biology, major in Cell Biology. Final dissertation: Molecular mechanisms cytokinin dependent necessary in the maintenance of staminal niche of Arabidopsis thaliana root meristem. Final mark: 110 cum laude/110. 2007 University of Rome Sapienza, Italy. B.Sc. degree in Biology, major in Genetics and Molecular Biology. Final dissertation: Auxins-Cytokinins: molecular dialogue at the transition zone of Arabidopsis thaliana root meristem. Final mark: 99/110. 08/01/2015 Pag. 1 of 5
Research Experience 2015 - Present: Research work as PostDoc in the laboratory of Prof. Sabrina Sabatini, Dept. of Research project: Meccanismi molecolari di controllo del differenziamento cellulare. Title of the research grant: Meccanismi molecolari di interazione tra citochinine e auxina nel meristema di Arabidopsis. Scientific sector: BIO/11 Scientific coordinator: Prof. Sabrina Sabatini. Main focus of the work: To investigate molecular mechanisms cytokinin dependent controlling auxin gradient establishment in the Arabidopsis root by combining mathematical, genetic and molecular high-throughput 2012 2014: Research work as PostDoc in the laboratory of Prof. Sabrina Sabatini, Dept. of Research project: Sviluppo del meristema radicale di Arabidopsis. Title of the research grant: Identificazione di segnali molecolari che controllano il differenziamento delle cellule staminali a distanza nel meristema di Arabidopsis. Scientific sector: BIO/11 Scientific coordinator: Prof. Sabrina Sabatini. Main focus of the work: To investigate molecular mechanisms cytokinin dependent controlling auxin gradient establishment in the Arabidopsis root by combining mathematical, genetic and molecular high-throughput 2009-2012: PhD Research training in the laboratory of Prof. Sabrina Sabatini, Dept. of Title of the Ph.D. project thesis: Novel mechanisms controlling auxin morphogenetic gradient in the Arabidopsis root meristem. (Supervisor: Prof. Sabrina Sabatini, Dept. of Biol. and Biotec. C. Darwin - Sapienza University of Rome; Tutor: Dr. Giovanna Frugis, Institute of Biology and Agricultural Biotechnology CNR, Rome). Di Mambro R. Main focus of the work: To investigate what are the molecular mechanisms acting cell-non-autonomously to control Arabidopsis root meristem size from 08/01/2015 Pag. 2 of 5
the lateral root cap by combining genetic and molecular high-throughput 2007 2009: Master research training in the laboratory of Prof. Sabrina Sabatini, Dept. of Final dissertation: Molecular mechanisms cytokinin dependent necessary in the maintenance of staminal niche of Arabidopsis thaliana root meristem (Supervisor: Prof. Sabrina Sabatini) Main focus of the work: To investigate the molecular mechanisms that control quiescent centre (QC) activity and therefore stem cell niche function by combining genetic and molecular approaches in Arabidopsis root meristem. 2007: Undergraduate training in the laboratory of Prof. Sabrina Sabatini, Dept. of Final dissertation: Auxins-Cytokinins: molecular dialogue at the transition zone of Arabidopsis thaliana root meristem. Main focus of the work: To investigate auxin and cytokinin crosstalk in the maintenance of the Arabidopsis root meristem activity. Fellowship and Awards 2011 EMBO Short term fellowship. Receiving Institute: Laboratory of Prof. Philip Benfey, Duke University (NC, USA). 2009 2012: Fellowship for excellence in academic performance of PhD School in Genetics and Molecular Biology - XXV cycle - Sapienza University of Rome, Italy. 2012 2014: Research grant - Dept. of Biology and Biotechnology C. Darwin - Sapienza University of Rome, Italy. 2015: Research grant - Dept. of Biology and Biotechnology C. Darwin - Sapienza University of Rome, Italy. Data processing knowledge - Proficient in Microsoft Office, including Word, Power Point, Excel and Access. - Expert in ImageJ, Adobe Illustrator and Photoshop CS2, CS5 and CS6. 08/01/2015 Pag. 3 of 5
- Proficient in online database PLAZA 2.5, Arabidopsis efp Browser, Biofuel Feedstock Genomics Resource (BFGR), The Arabidopsis Information Resource (TAIR), Salk Institute Genomic Analysis Institute (SiGnAL), Plant Transcription Factor Database (PlnTFDB 3.0), The European Arabidopsis Stock Centre (unasc), ATTED-II 7.1, Promomer and BioMart Central Portal. - Expert in High-throughput Data analysis software T-MeV, Chipenrich-1.0. - Expert in Confocal microscopy, trained with Leica SP2, Zeiss LSM 500-510-700-710-780. - Skilled in software and hardware management. - Proficient with image analysis software Cell-o-Tape and Cellset. - Skilled in BioLayout Express3D and Cytoscape softwares designed for visualization, clustering, exploration and analysis of network graphs in two- and three-dimensional space derived from biological data. Participation at meetings and workshops May 2012 EMBO International Conference on Plant development and environmental Interactions, Matera (Italy). Abstract: Di Mambro R, Perilli S, Sozzani R, Pierdonati E, Benfey PN, Costantino P, Sabatini S. (2012) Tissue specific cross-talk between cytokinins and auxin is critical for Arabidopsis root meristem growth and development. Abstract: Moubayidin L, Di Mambro R, Pacifici E, Inez Terpstra, Perilli S, Dello Ioio R, Heidstra R, Costantino P and Sabatini S. (2012) SCARECROW sustains stem cell activity inhibiting cytokinin dependent cell differentiation input. Abstract: Perilli S, Perez-Perez JM, LLavata Peris C, Di Mambro R, Moubayidin L, Costantino P, Scheres B and Sabatini S. (2012) RETINOBLASTOMA-RELATED protein and cytokinin interaction during root meristem cell differentiation. December 2012 Auxin 2012, Hawai i, The Big Island (USA). Abstract: Di Mambro R, Perilli S, Sozzani R, Pierdonati E, Benfey PN, Costantino P, Sabatini S. (2012) Tissue specific cross-talk between cytokinins and auxin is critical for Arabidopsis root meristem growth and development. June 2014 International Symposium 2014 on Auxin and Cytokinins in Plant Development, Prague (Czech Republic). Abstract: Di Mambro R,, De Ruvo M, Perilli S, Sozzani R, Pacifici E, Benfey P, Wolfgang B, Costantino P and Sabatini S. (2014) Tissue specific cross-talk 08/01/2015 Pag. 4 of 5
between cytokinins and auxin is critical for Arabidopsis root meristem growth and development. Abstract: Pacifici E, Di Mambro R, Sozzani R, Benfey P, Wolfgang B, Costantino P, Sabatini S. (2014) Molecular mechanisms of interaction between cellular expansion and cytokinin signaling. Publications Moubayidin L, Di Mambro R, Sabatini S. (2009) Cytokinin-auxin crosstalk. Trends Plant Science. 14:557-62. Moubayidin L, Di Mambro R, Perilli S, Dello Ioio R, Costantino P, Sabatini S. (2010) The rate of cell differentiation controls the Arabidopsis root meristem growth phase. Current Biology. 20:1138-43. Aichinger E, Villar CB, Di Mambro R, Sabatini S, Köhler C. (2011) The CHD3 chromatin remodeler PICKLE and polycomb group proteins antagonistically regulate meristem activity in the Arabidopsis root. Plant Cell. 23:1047-60. Di Mambro R, Perilli S, Sabatini S. (2012) Growth and development of the root apical meristem. Current Opinion in Plant Biology. 15:17-23. Moubayidin L, Di Mambro R, Sozzani R, Pacifici E, Salvi E, Terpstra I, Bao D, van Dijken A, Dello Ioio R, Perilli S, Ljung K, Benfey PN, Heidstra R, Costantino P, Sabatini S. (2013) Spatial coordination between stem cell activity and cell differentiation in the root meristem. Developmental Cell. 26:405-15. Perilli S, Perez-Perez JM, Di Mambro R, Peris CL, Díaz-Triviño S, Del Bianco M, Pierdonati E, Moubayidin L, Cruz-Ramírez A, Costantino P, Scheres B, Sabatini S. (2013) RETINOBLASTOMA-RELATED protein stimulates cell differentiation in the Arabidopsis root meristem by interacting with cytokinin signaling. Plant Cell. 25:4469-78. 08/01/2015 Pag. 5 of 5