Table Title Page. 1 World petrochemical production 5. 3 Petrochemicals and its applications 9. 4 The major products ox I.P.C.L. 15

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LIST OF TABLES Table Title Page S o. i'i o 1 World petrochemical production 5 2 World Petroleum and natural Gap, Resources 7 3 Petrochemicals and its applications 9 4 The major products ox I.P.C.L. 15 5 IPCL*s share of total installed, capacity in selected products 17 6 Pollutants in petrochemical industry wastewater 20 7 Toxicity of petrochemical pollutants 22 8 Influence of structure on degradability 47 9 Microorganisms detected in different sewage treatment plants 49 10 Microorganisms utilizing petroleum hydrocar cons and petrochemicals 50 11 Major Genera of hydrocarbanoclastic bacteria and fungi from marine and brackish water 66 12 Plasmid-coded degradative pathways of soil bacteria 79 13 naturally occuring nitriles 81 14 Industrial applications of nitriles 84 15 List of different nitriles utilizing microorganisms 89 16 Accumulation of heavy metals ir. microorganisms 100 17 Threshold of inhibitory effects on activated sludge process 105 18 List of chemicals used and source 110 19 Reagents and standards used for various estimations 111 Contd.,

Tabl do. 0 Title Pacre 20 C omposition of Fogg's Medium 12B 21 Zarrauk' s Medium 129 22 Composition of Basal Salt Medium 130 23 Composition of Mac Conk ay's agar 131 24 Composition of Nutrient near 132 25 Compos! ti on of Urea Agar Medium 133 26 Compositi on of Kenkright and Kunaiers Medium 134 27 Composition of Ashby's Mannitol agar medium 135 28 Composition of Nile medium 136 29 Comoosit ion of Delta medium 137 30 C omposition ox Masurovsky' s medium 138 31 Composition of Algae medium 139 32 Composition of Luria-Bertani (IB) medium 140 33 Composition of Rose-Bengal Agar 141 34 Composition of Synthetic medium-y 142 35 Details of assay system for bacterial enzymes 149 36 Characteristics of the wastewater samples collected at different points in IPCL central wastewater treatment plant 158 37 Plate counts of bacteria and fungi in different culture media inoculated with samples collected from different points from IPCL wastewater treatment plant 160 38 Characteristics of bacteria isolated from IPCL wastewaters 162 39 Bacteria isolated from IPCL wastewater treatment plant 171 4o Screening of bacteria isolated from IPCL wastewater s for petrochemical utilization 172 Contd..

Table Title Page No, -.41 Screening of bacteria isolated from IPCL wastewaters for heavy metal tolerance 178 42 Composition of the chemical constituents in the combined IPCL wastewaters 182 43 Growth of Algae in the presence of organic chemicals 185 44 Growth of algae in the presence of heavy metals 185 45 Growth of bacteria used for combined IPCL wastewater treatment on organic chemicals 188 46 Growth of bacteria used for combined IPCL wastewater treatment in presen ce of heavy metals 188 47 IFCL combined wastewater characteristics 189 48 Treatment of combined IPCL wastewater by selected cultures of bacteria and algae 190 49 Composition of IMT wastewater 192 50 Growth of bacteria used for EMT plant effluent treatment on organic chemicals 193 51 Growth of bacteria used for HIT plant effluent treatment in presence of heavy metals 193 52 Characteristics of HIT wastewater 1S4 53 Treatment of DMT waste in laboratory using mixed culture of bacteria ' 196 54 Effect of treated & untreated Q-IT plant wastewater on the growth of embryo during germination 199 55 Effect of treated & untreated IMP plant wastewater on protease activity during germination 200 56 Effect of treated & untreated IMT plant wastewater on protein content of attached cotyledons during the germination 201 57 ACN-ACR' wastewater characteristics 204 Contd.,

Table Title Page T,*T _ A O. 58 Growth of bacteria used for ACIJ plant effluent treatment on organic chemicals 205 59 Growth of bacteria used for ACLI plant effluent treatment in the presence of heavy metals 205 60 Treatment of ' ACKuaCR' wastewacer 206 61 Effect of treated & untreated acn plant wastewater on protease activity during germination 209 62 Effect of treated & untreated acetonitrile-acrylonitrile plant waste water on protein content of attached cotyledons during gemination 210 63 Effect of untreated & treated nctt plant wastewater on the growth of embryo during germination 212 64 Bacteria, utilizing acetonitrile 213 65 Grower of bacteria isolated from IPCL effluents at different concentrations of Acetonitrile 215 66 Stoichiometry ox acetonitrile hydrolysis by cellfrae extract 218 67 Stoichiometry of acetamide hydrolysis by cell free extract 220 68 Effect of period of incubation on the breakdown of acetonitrile and production of nitrilase and am id use by Azotobacter sp. P23-105 222 69 Effect of initial pk of the medium on growth and production of nitrilase and amidase by Azotobacter sp. P23-105 224 70 Effect ox temperature of incubation on bacterial growth and nitrilase and amidase production by Azotobacter sp. P23-105 225 71 Effect of amount ox inoculum on growth and nitrilase and amidase production by Azotobacter sp. P23-1C5 226 Contd

Table Title Page 72 Effect of shaking during cultivation on growth and production of nitrilase and amilase by A zotobacter sp. P23-105 73 Induction of nitrilase and amidase in the cells of azotobacter sp. P23-105 Ik Growth and enzyme production by Azotobacter sp. P23-105 on various nitriles ard amides 75 Effect of acetonitrile concentration on growth and nitrilase and amidase oroduction by A zotobacter so. P23-105 76 Effect of ph: on nit rilase activity1 77 Effect of enizyme CO. n cant rat ion on nitrilase activit 78 Effect of :ri od of incubation on nitrilase activit 79 Effect of su bst rate cone ent ration on nitrilase activity 80 Effect of temperature on nitrilase activity 81 Effect of heat inactivation or. nitrilase activity 82 Effect of various inhibitors cn nitrilase activity 83 Comparison of the characteristics of nitrilase from various sources 84 Effect of ph on araidase activity 85 Effect of temperature on amidase activity 86 Effect of heat inactivation on amidase activity 87 Effect of enzyme concentration on amidase activity 88 Effect of period of incubation on amidase activity 89 Effect of substrate concentration on amidase activity 90 Effect of various inhibitors cn amidase activity 227 229 230 231 233 235 237 239 243 247 249 251 252 254 256 260 262 264 268 Contd

Table Ho. 1 i 1 -i""1 tj. Page Ho. 91 activities of the enzymes involved in ammonia assimilation in Azotobacter sr. P23-10 5 grown on different carbon and ni'trogen sources 272 92 Activities of the enzymes involved in ammonia assi&ilation in Azotobacter sp. P23-105 grown on acetonitrile as sole source of carbon and nitrogen 273 93 Resistance of Azot obacter sp. P23-105 to heavy metals and anttbyotics 277 94 Properties of wild and cured strains of Azot obacter so. P23-105 " 281 95 Characteristics of yeast isolate 283 96 Growth of Rhodotorula sp. Pxy on organic chemicals 234 97 Growth of Rhodotorula sp. Pxy in presence of Heavy metals 285 98 Toxicity of heavy metals on Rhodotorula sp. Pxy 237 99 Growth of Rhodotorula sp. Pxy in the presence of lead salts 239 100 Growth of Rhodotorula sp. Pxy in the presence of lead acetate 291