Product Knowledge Filter Vials PTFE PVDF Nylon PES.2µm.45µm.2µm.45µm.2µm.45µm.2µm.45µm Pat. US #7,790,117 Worldwide patents pending Thomson Instrument Company Toll Free 800 541.4792 Email folks@htslabs.com Local 760 757.8080 Fax 760 757.9367 Online www.htslabs.com Address 1121 South Cleveland Street Oceanside, CA 92054
Background Information Background on Chromatography: One takes a dirty sample, places into the outer shell, then presses the plunger. Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound s partition coefficient result in differential retention on the stationary phase and thus changing the separation. Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for further use (and is thus a form of purification). Analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture. The two are not mutually exclusive. liquid. Liquid chromatography can be carried out either in a column or a plane. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC). Gas chromatography For more details on this topic, see Gas chromatography. Gas chromatography (GC), also sometimes known as Gas- Liquid chromatography, (GLC), is a separation technique in which the mobile phase is a gas. Gas chromatography is always carried out in a column, which is typically packed or capillary (see below). Youtube section: Chromatography Basics Learning the basics on HPLC Learning the basics on GC How an autosampler works? UHPLC guide They are used on HPLC (high pressure liquid chromatography), UHPLC ( ultra high pressure liquid chromatography), and by GC. Liquid chromatography (LC) is a separation technique in which the mobile phase is a Thomson Instrument Company is not affiliated with YouTube or any of their products.
00.5 HPLC Through-put Look at the Pump as a flowing river of clean water or solvent The Auto Sampler takes a sample from the Filter Vial (or HPLC vial) Passes the sample through a Column The sample is detected here A colection is made from the sample run and waste is seperated a b c d Pump flows 50-5000µL/min) Auto Sampler w/ Filter Vial Detector Fraction Collector Waste 300 1 2 1.Fucose 2.Gaal ctosamine 3.Gul cosamni e 4.Galactose 5.Gul cose 6.Mannose HPLC Column in Oven mv 3 4 5 6 0.00 Minutes 20.00 Control & Data Processing
Specifications Filter Vial is like a French press for on-line sample preparation for HPLC. Filter vial is a simple tool to do extraction of solids out of a liquid before injecting your liquid onto a chromatography system. The solids are needed to filter out because just like an engine small parts can clog the total machine and cause problems multiple places. An oil filter catches particles before they go into an engine, a filter vial catches dirty particulates before it goes onto the HPLC, or GC. With the addition of UHPLC ( ultra high pressure liquid chromatography) the lines of tubing are 1/32 thick, and the HPLC columns are 2um, so any particles can clog the machine much more easily. Filter vial composition: Material composition made of the filter vial is Polypropelene, lc/ms tested by Pfizer and Takeda for leechables. 32 MM SET NEEDLE DEPTH TO 5 MM FROM BOTTOM FILL LINE VOLUME 450 UL DEAD VOLUME 120 UL 12 MM NOTES: All Tolerances +/-.005. Unless otherwise noted. Size: A Scale = 1:1 PROJECT: TITLE: REV: APPROVED: FILTER VIAL.45µL 8-11-A
Membranes: PTFE PVDF Nylon PES.2µm.45µm.2µm.45µm.2µm.45µm.2µm.45µm PTFE (Teflon) Used for Organic material. Anything that is using 80% organic or above. This is used in medicinal chemistry, paint, power lines. PVDF (Polyvinylidene Fluoride) Organic and Aqueous 2nd most chemically resistant to Teflon. Used in food, corn applications, proteins from microbes (Microbes defined as e.coli, fungi, yeast), pesticides Nylon Organic and Aqueous. Most used for environmental labs. Older membrane technology PES (Polyethersulfone) Aqueous mostly Used for secreted material, such as antibody, and other secreted proteins. Secreted means the protein is spit out of the cell into the soup. Mammalian cell (CHO and HEK293), and insect cells secret proteins. Type Glass Microfiber (GMF) Nylon (NYL) Polyethersulfone (PES) Polypropylene (Dp PP) Polypropylene (PP) Polytetrafluoroethylene (PTFE) Polyvinylidene difluoride (PVDF) Regenerated Cellulose (RC) Applications High loading capacity, binder-free, glass microfiber depth filter meduim Membrane for aqueous and organic samples within a ph range of 3-10. For aqueous-based samples. Good flow rates. Aqueous and oraganic samples; high loading capacity depth filter. For solvent-based samples, low water breakthrough values. For aggressive samples. Great chemical compatibiltiy Low nonspecific protein binding membrane for samples in aqueous solutions or organic solvents. Suitable for use with either aqueous or organic solvents. Very low nonspecific protein binding membrane. Thomson Instrument Company is not affiliated with Bodum or any of their products.
Caps Some are not like the others Low evaporation cap Silicone/PTFE: No Slit, good for GC, Pesticides, solubility testing, degradation testing. Does give low evaporation.2-.3% per day. Septa tested for MAss spec. Pre-slit cap Silicone /PTFE: Easier for waters system, great for open access mass spec. for medicinal chemistry labs, and aqueous applications. Gives higher evaporation 3-4% per day. Not usable on GC or low boiling solvents (methanol), and also tests running for a long time. Septa tested for Mass spec.
Replace syringe filter, syringe, hplc vial, and cap Takes 15 seconds, versus syringe filters which can take 3 minutes to do Filter vials allow dirt or particulates to go to the bottom Syringe filters you are using force to push the liquid through. Clogging occurs and you can have explosions of a precious sample Confines aerosols from filter vials Easy to press with hand Caps are color coded for easy identification Eco-friendly by using a lot less parts Disposal and neutralization of a lot less parts, hazardous chemical waste is expensive Lab space is minimized. Lab space is a premium!!! Minimizes sample loss
Syringe Filters Emit Dangerous Aerosols! Customers Are Mostly Concerned About Safety Priming Syringe Holds 450µl, with 120µL of dead volume Has pre-slit cap for easy needle piercing (these come standard) Available in multiple membranes.2µm and.45µm Comes in packs of 200 and 500 pieces Syringe Filter Blockage & Explosions The needle depth on the autosampler must be set to 5mm. If it is set lower it could bottom out and damage the needle. µl= micro liter It is 12mmx32mm which is a standard 2mL autosampler vial size
Filter Vials Come in packs of Patent No. 7,790,117 200 & 500 pieces.5 Molded together with Elastic Living Hinge 2 Patent No. 7,790,117 Patent No. 7,790,117 Inset Into Texture 7 5.5 Inset Into Texture
Machine Capability: Waters Acquity has a color cap issue with their UHPLC. If they can not see the color cap then you need to talk to Waters and they will send out a fix. Waters Acquity Agilent /Waters is 90% of the autosamplers. It works on both. Waters prefers pre-slit because their needles are thicker and can clogg the septa. Shimadzu, Thermo, Perkin elmer, etc. compatible as long as they take 12mmx32mm vials in the autosampler GC is agilent systems. Agilent Technologies Thomson Instrument Company is not affiliated with Waters or their product the Acquity, or Agilent Technologies or their product the 1200 Series.
Optimized Products Hand pressure to push down is 50% less than GE Mini-Uniprep, our competitor Color coded caps for different membranes Safety versus syringe filter confining aerosols, and minimizing explosions Eco-friendly by using a lot less parts Disposal and neutralization of less parts, hazardous chemical waste is expensive Lab space is minimized. Takes 15 seconds, versus syringe filters which can take 3 minutes to do.
Think Green Filter Vials Minimize Waste Replaces: Syringe filter Syringe HPLC vial & cap Additional Parts Needed: Syringe + HPLC Vial + HPLC Cap Lab Space Waste Containers Minimal Parts Needed: No Exrtra Parts Needed Lab Space Waste Containers Filter Vial Time=$ Timing is $100 an hour for labor in chemical industry. Time is more expensive than parts. Thomson Instrument Company is not affiliated with The Price is Right or any of their products.
Colleagues Recommend This Product Look at all the fancy names in this list! We work closely with and receive data from Takeda Pfizer -Antibody Data Astra Zeneca Roche (Genentech )-Real Time Fermentation APnote GSK Thomson Instrument Company is not affiliated with Pfizer, Roche-Genentech, Astra Zeneca, GSK, Takeda or any of their products.