Empower 3 Control of Instruments 3rd Party Control

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Empower 3 Control of Instruments 3rd Party Control Mogens Hallas Nordic User Training 2011 Långvik, September 6 th to 9th 2011 Waters Corporation 1

2011 Waters Corporation 2

Empower 3 Obsoleted support of detectors The following instrumentation is reaching its end of life, is on limited support status and will no longer be supported in Empower 3: - 410 RI Detector - 486 TUV Detector - 996 PDA Detector - 474 Fluorescence Detector Support for the A5890S and 7673S under serial control (Using LAN Serial Control) But not serial buslac/e control, will remain in Empower 3. 2011 Waters Corporation 3

Equinox Serial 8-Port Card Equinox Serial Card is only supported on Windows XP 32-bit Not in Windows 7. Current serial instrument support using the Equinox 8-port card is: - Agilent 5890 GC (5890S) - Agilent 7673 GC autosampler (7673S) - Agilent 6890 GC (all models, including 6890N) - Agilent 6850 GC (serial number <=US00003200) - Agilent 7694 Headspace Sampler - Waters bussat/in module - Waters 474 fluorescence detector - Agilent A1100 LC modules (not A1200 modules) A new LAN based serial device is replacing the Equinox Serial Card 2011 Waters Corporation 4

Prior to Empower 3 Previously discontinued instrumenys As a reminder, support for the following instruments has been previously been discontinued: Not supported as of Empower 2 - Integrity TMD - ISA BusLAC/E card - GPC 2000-5890 GC (under buslac/e control) - 7673 (under buslac/e control) Not supported as of Empower build 1154-700, 710, and 712 Injectors - 715 Autosampler - LC Module 1 Not supported as of Millennium32 v4.0 - Waters 490 Variable UV 2011 Waters Corporation 5

Instrument Control Provide a comprehensive, reliable and robust feature set for each instrument we support. Waters works closely with its customers to ensure the instruments supported with Empower Software provided scientist the capabilities to realize the most value from their instrument investment. Instrument Driver Approach - Open Instrument Portal (OIP) Similar to the Microsoft Printer driver approach Allows creation of software plug-ins to add hardware control capabilities Waters uses and other instrument vendors to create instrument control 2011 Waters Corporation 6

What is OIP Technology? Open Interface Portal (OIP) technology is a software architecture that allows for a standardized specification of the interaction between the Data System and an instrument. This allows for the development of an instrument driver that can be added to Empower Software without changing the core application. OIP technology is separate from the physical connectivity (serial, ethernet, USB, IEEE-488) OIP technology takes advantage of other software specifications: COM, ActiveX, XML 2011 Waters Corporation 7

Extended Instrument Control Using OIP with Empower Software One software solution for all chromatographic systems Reduced training, validation and support 16 vendors with over 235+ instruments Instrument control available from major LC Vendors including Waters, Agilent, Shimadzu, Hitachi and Dionex *) Instrument Control Available from major GC Vendors including Agilent, Shimadzu, PerkinElmer and Varian New instrument control Waters ACQUITY H-Class Waters Thar SFC Beckman CE WFC III *) some are still in testing 2011 Waters Corporation 8

Instrument control via OIP Created by Waters HPLC systems and module, ACQUITY UPLC, Single-quad & Triple-quad MS, A/D Converter, Agilent 6850 GC, Agilent 7694 Headspace Sampler, Agilent 1100, 1200 and 1260/1290 series LC modules Created by Other Instrument Vendors CTC PAL Autosamplers Varian 3900 GC Perkin-Elmer GC Tekmar HT3 Headspace Sampler Dissolution Baths o Hanson SR8-Plus, Distek (2100C, Evolution 6100 & 6300), Sotax and Erweka DT800 Online System Selerity Polaratherm 9000 Shimadzu Prominence LC 20 System Hitachi LaChrom Elite Systems Dionex 2011 Waters Corporation 9

Complete list of all controlled systems 2011 Waters Corporation 10

Third Party Instrument Control in Empower 3 Waters policy is to notify their software partners when a new informatics software product is under development. The Empower 3 team has notified all of our partners about Empower 3 and has offered beta software for use in testing /development of ICS software, for Empower 3. To date not all vendors have requested Empower 3 beta or released software. We have recently reminded our partners that the release of Empower 3 software and we would like to offer our mutual support of their products. We will contact our partners again. Please refer users who are requesting third party instrument control to the appropriate vendors to request support of these instruments in Empower 3. 2011 Waters Corporation 11

Instrument control Consistent instrument interface assist in training new software users as new techniques such as Gas Chromatography (GC) or Ultra Performance Liquid Chromatography (UPLC) are introduce to the laboratory. 2011 Waters Corporation 12

Instrument Control Once instrument parameters are entered into the Empower software they can be completely managed by the software to ensure that these same parameters are used for all future analysis. If changes are required, all instrument parameters are completely documented in Empower's embedded relational database with complete audit trail including what changes were made, who made the changes, when the changes were made and why. 2011 Waters Corporation 13

Waters HPLC s Full control of Waters binary and quaternary pumps. Full control of Multiple wavelength, Photodiode array, Fluorescence, and RI detectors. Full Control of sample compartment and column oven temperatures Full control of autosamplers. Full control of Waters single quad mass detectors. 2011 Waters Corporation 14

Waters UPLC Full control of Waters binary and quaternary pumps. Full control of Multiple wavelength, Photodiode array, Fluorescence, and RI detectors. Full Control of sample compartment and column oven temperatures Full control of autosamplers. Full control of Waters Mass Spec detectors. 2011 Waters Corporation 15

Waters Mass Detectors Data acquisition and instrument control of Mass Detectors. Greater specificity of analysis. Easily use MS and MS/MS in QC and Development lab environments. 1st Chromatography Software to offer Tandem control and data processing in a network or regulated environment 2011 Waters Corporation 16

Mass Detection Waters SQD & TQD Data acquisition and instrument control of Mass Detectors Greater specificity of analysis Easily use MS and MS/MS in QC and Development lab environments 1 st Chromatography Software to offer Tandem control and data processing in a network or regulated environment. 2011 Waters Corporation 17

Supercritical Fluid Chromatography (SFC) Thar Method Station & SFC-MS Resolution Systems Analytical to semi-preparative purification solutions Control includes: Fluid Delivery Module and co-solvent selection Photodiode Array, UV & ELSD detectors Column oven and column selection Automated Back Pressure Regulator Thar Autosampler Waters 3100 MS detectors Waters Fraction Collector III 2011 Waters Corporation 18

ACQUITY UPSFC System 2011 Waters Corporation 19

Dissolution Baths Integrity of your HPLC dissolution testing methods and results, helping increase lab productivity. Combined software and hardware solution. Hanson SR8-Plus Distek 2100C, Evolution 6100 & 6300 Sotax Erweka DT800 Online Dissolution System Vankel VK 7000 Vankel VK 7010 2011 Waters Corporation 20

Agilent 1100/1200/1260/1290 LC Full control of Isocratic, Binary and Quatenary pumps. Full control of Autosampler. Full control of UV, MWV, RI, FLD and DAD detectors Full control of column oven. 2011 Waters Corporation 21

Agilent 5890 Series II GC Control with either 7673 or G1512 Controller Dual Tower capability (With serial card only) Detector Supported FID,NPD,TCD,ECD Sampling Rates of 1.25, 2.5, 5, 10, 20 points per second. Fully Programmable temperatures: limits, ramps, and hold times. Injector inlet zone temperature setting. 2011 Waters Corporation 22

Agilent 6890 / 6890N GC 6890 supported in both Single and Dual Tower mode. Sampling Rates of 0.1, 0.2, 0.5, 1, 2, 5, 10, 20, 50, 100, and 200 points per second. Fully Programmable temperatures: limits, ramps, and hold times Detector options: Unknown (Other detectors) FID (Flame Ionization) TCD (Thermal Conductivity) NPD (Nitrogen Phosphorous) ECD (Electron Capture) uecd (Micro Electron Capture) FPD ( Flame Photometric) 2011 Waters Corporation 23

Agilent 6850 GC Inlet: Split/Splitless & Purged Pack limits, ramps, and hold times Cool On-Column inlet project in progress Detector options: FID (Flame Ionization) TCD (Thermal Conductivity) 2011 Waters Corporation 24

Agilent HS7694 Headspace Sampler Introduced July, 2003 The first Headspace/GC system control option in a compliance-friendly CDS G1289B and G1290B 2011 Waters Corporation 25

Agilent G1888 Headspace Sampler Headspace Control Option Will continue support for the 7694 Will add support for the G1888 Will allow remote operation through a LAC/E module 2011 Waters Corporation 26

Tekmar HT3 GC Headspace Tekmar HT3 Static and Dynamic Headspace System driver available from Teledyne Tekmar Static Headspace analysis: robust technique for volatile compounds analysis of in almost any matrix. Dramatic improvements in sensitivity by new Dynamic Headspace option, while maintaining ruggedness and reliability of traditional Static Headspace. 2011 Waters Corporation 27

Varian 39XL GC Drivers available from Varian Product Sales Single 1177 split/splitless or 1041 injector equipped with Injector Electronic Flow Control, FID or TCD detector with Electronic Flow Control Option: keypad and Ethernet communications 2011 Waters Corporation 28

CTC PAL Injection Systems LC PAL, GC PAL and Combi PAL drivers available from CTC Analytics Automated GC and LC injection systems Outstanding performance and maximum flexibility in every LC sample processing system Provides powerful working capabilities and maximum flexibility in every GC sample processing system Combi PAL, the only GC sample injection system combining liquid, large volume, headspace, in tube extraction in a single instrument. 2011 Waters Corporation 29

Beckman CE Systems Beckman P/ACE MDQ & PA 800 Capillary Electrophoresis Systems Control includes photo diode array and selectable wavelength UV/Vis (with 200, 214, 254 and 280 nm filters) detector, UV source optics, temperature-controlled sample storage module and LIF Detector Beckman CE Control License includes instrument control software, license, documentation, and an IEEE-to-Ethernet converter to connect Beckman CE (IEEE-488) via Ethernet Support for the LIF (Laser-Induced Fluorescence) detector 2011 Waters Corporation 30

Selerity Polaratherm 9000 Total Temperature Controller Selerity Polaratherm 9000 Total Temperature Controller o Temperature programmed HPLC is taken to extremes with the Polaratherm Series 9000 Total Temperature Controller with mobile phase preheating, and optional cryo cooling. 2011 Waters Corporation 31

Shimadzu Prominence LC 20 System Prominence LC 20 drivers available from Shimadzu Availability expected 3Q, 2007 Supported Modules System Controller - CBM-20A, CBM-20A lite Pump - LC-20AD, LC-20AT, LC-20AB Autosampler - SIL-20A, SIL-20AC, Rack Changer, Rack Changer/C Column Oven - CTO-20A, CTO-20AC Detector - SPD-20A, SPD-20AV, SPD-M20A (PDA) Solenoid valve - LPGE unit built in LC-20AD/20AT Rotary valve - FCV-12AH installed in CTO-20A/20AC Rotary valve - FCV-14AH installed in CTO-20A/20AC Analog input - PC-55N 2011 Waters Corporation 32

Shimadzu GC GC System Shimadzu GC-2010 GC System Shimadzu GC-2010 Plus GC System Shimadzu GC-2014 GC Autosampler Shimadzu AOC-20i GC Autosampler Shimadzu AOC-20i/s 2011 Waters Corporation 33

PerkinElmer Clarus GC PerkinElmer Clarus 400 GC PerkinElmer Clarus 500 GC PerkinElmer Clarus 600 GC 2011 Waters Corporation 34

Perkin Elmer GC and HS TurboMatrix Headspace samplers 2011 Waters Corporation 35

Hitachi LaChrom Elite Systems LaChrom Elite System drivers available from Hitachi Availability expected 4Q, 2007 Supported Modules L-2100 and L-2130 - Pump L-2200 - Autosampler L-2300 and L-2350 - Column Oven L-2400 - UV Detector L-2420 - UV / Vis Detector L-2450 and L-2455 - Diode Array Detector L-2480 and L-2485 - Fluorescence Detector L-2490 - Refractive Index Detector AID - Analog Input Device (Single Channel) 2011 Waters Corporation 36

Hitachi LaChromUltra Hitachi LaChromUltra L-2160U Pump Hitachi LaChromUltra L-2200U Autosampler Hitachi LaChromUltra L-2400U UV Detector Hitachi LaChromUltra L-2420U UV-Vis Detector Hitachi LaChrom L-2455U Diode Array Detector Hitachi LaChromUltra L-2485U Fluorescence Detector 2011 Waters Corporation 37

ESA Biosciences ESA pump, ESA Coulochem III electrochemical detector, ESA Corona, Corona Plus, Corona Ultra CAD detectors, CAD v1.23 Detector Corona Plus V1.23 Corona Ultra V1.05 2011 Waters Corporation 38

Dionex UltiMate 3000/ UltiMate 3000/3000 RSLC LPG-3400SD Quaternary Analytical pump UltiMate 3000/3000 RSLC LPG-3400BM Quaternary Biocompatible Micro pump UltiMate 3000/3000 RSLC DGP-3600 pumps UltiMate 3000/3000 RSLC DGP-3600RS rapid separation dual low-pressure gradient pump UltiMate 3000/3000 RSLC DGP-3600SD analytical dual low-pressure gradient pump UltiMate 3000/3000 RSLC DGP-3600BM biocompatible dual low-pressure gradient pump UltiMate 3000/3000 RSLC HPG-3x00 pumps UltiMate 3000/3000 RSLC HPG-3x00RS rapid separation high-pressure gradient pump UltiMate 3000/3000 RSLC HPG-3x00SD analytical high-pressure gradient pump UltiMate 3000/3000 RSLC ISO-3100 pumps UltiMate 3000/3000 RSLC ISO-3100SD analytical isocratic pump UltiMate 3000/3000 RSLC ISO-3100BM biocompatible isocratic micro pump UltiMate 3000/3000 RSLC FLM-3x00 flow managers and termostatted column compartments UltiMate 3000/3000 RSLC TCC-3X00(RS) thermostatted column compartments UltiMate 3000/3000 RSLC ACC-3000 autosampler column compartment UltiMate 3000/3000 RSLC WPS-3000(RS) autosamplers UltiMate 3000/3000 RSLC DAD-3000(RS) diode-array detector UltiMate 3000/3000 RSLC FLD-3x00(RS) fluorescence detector UltiMate 3000/3000 RSLC MWD-3000(RS) multi-wavelength detector UltiMate 3000/3000 RSLC VWD-3x00(RS) variable-wavelength detectors 2011 Waters Corporation 39

Dionex IC systems Control of Dionex range of IC systems under development ICS-5000 Reagent-Free IC System ICS-2100 Integrated Reagent-Free IC System ICS-1600 Standard Integrated IC System ICS-1100 Basic Integrated IC System ICS-900 Starter Line IC System 2011 Waters Corporation 40

Extended Instrument Control with Empower Software Benefit to the Users: One Software solution for all chromatographic systems Reduced Training, Validation and Support Instrument Control Available Today 16 Vendors with 235+ Instrument modules Major LC and GC Vendors New Techniques Supercritical Fluid Chromatography (SFC) Capillary Electrophoresis (CE) 2011 Waters Corporation 41

2011 Waters Corporation 42