Cedex Bio HT Bioprocess Analyzer Be Confident. Be Assured.
Next Generation Technology for Bioprocess Analytics Analyze Your Samples with Confidence. Be Assured of the Data Integrity. Metabolite Screening Substrate Analysis Fermentation Analysis At-Line Monitoring Quality Reliable photometric technology based on 40 years of experience High accuracy and precision for entire assay range Broad dynamic range and sensitivity Performance superior to membrane technology 5% variability between instruments and reagent lots Speed Automated daily maintenance Pre- and post-dilution capabilities Long reagent on-board stability Reagent loading capacity of 32 cassettes 90 samples loaded at one time Average maintenance is just about 100 minutes per year Value Versatile menu tailored to today s workload Expandable menu for tomorrow s requirements One instrument for multiple parameters of mammalian, bacterial, and yeast culture systems Minimal hands-on time: a true walk-away analyzer Consistent results using the Cedex Bio and Cedex Bio HT Bioprocess Analyzers CEDEX and ISE are trademarks of Roche. For use in quality control/manufacturing processes only.
Performance Characteristics Process Control with Maximal Reliability. Comparison of Cedex Bio HT and Membrane Technology in Glucose Analysis Cedex Bio HT instrument data demonstrate greater accuracy and precision (1, 2), sensitivity (3) and linearity (4) compared to instruments using membrane-based amperometric technology. Measuring Ranges of the Tests Analyte Test range With automatic dilution up to Acetate Ammonia (NH3) 0.17 25.4 mmol/l 10 1500 mg/l 0.28 13. 9 mmol/l 4.73 236 mg/l 2.54 mol/l 150 g/l 1.39 mol/l 23.6 g/l Recovery [%] 140 130 120 110 (3) (4) 100 90 80 (1,2) Calcium Cholesterol Galactose Glucose 0.2 5 mmol/l 8 200 mg/l 0.01 2.07 mmol/l 3.87 800 mg/l 0.06 39 mmol/l 0.01 7 g/l 0.11 41.6 mmol/l 20 7500 mg/l 500 mmol/l 20 g/l 207 mmol/l 80 g/l 3.9 mmol/l 700 g/l 4.16 mol/l 750 g/l 70 60 50 40 0 1000 2000 3000 4000 5000 6000 7000 Target Concentration [mg/l] Membrane analyzer Cedex Bio HT Figure 1: Recovery of glucose concentration was determined in parallel using the Cedex Bio HT System and a membrane analyzer. Recovery is calculated as percentage of the measured value to the respective target value of a standard solution. A total of 250 measurements of standards with different concentrations was made over a six month period. Glutamate Glutamine Glycerol IgG Iron Lactate 0.1 10 mmol/l 15 1500 mg/l 0.1 10 mmol/l 15 1500 mg/l 0.1 10 mmol/l 9.21 921 mg/l 0.067 10.7 µmol/l 10 1600 mg/l 0.9 179 μmol/l 0.05 10.0 mg/l 0.04 15.5 mmol/l 4 1400 mg/l 1 mol/l 150 g/l 1 mol/l 150 g/l 1 mol/l 92.1 g/l 1.07 mmol/l 160 g/l 17.9 mmol/l 1 g/l 1.55 mol/l 140 g/l High Correlation for the Cedex Bio and Cedex Bio HT Analyzer Cedex Bio HT 9000 8000 glucose [mg/l] 7000 6000 5000 4000 y = 1.002x 3000 R 2 = 0.998 2000 1000 0 0 1000 2000 3000 4000 5000 6000 7000 8000 9000 Cedex Bio Figure 2: The Cedex Bio and Cedex Bio HT Systems show the same recovery of glucose. LDH 20 1000 U/L 100 ku/l Magnesium Phosphate Potassium (ISE) Pyruvate Sodium (ISE) 0.15-2.5 mmol/l 3.65-60.8 mg/l 0.1-8.36 mmol/l 9.5-795 mg/l 0.2-30 mmol/l 0.008-1.17 g/l 0.1-8 mmol/l 8.7-696 mg/l 20-250 mmol/l 0.46-5.75 g/l 250 mmol/l 6.08 g/l 836 mmol/l 79.5 g/l 800 mmol/l 69.6 g/l Total Protein 2-120 g/l max. solubility
System Specifications High-level System Robustness. Test Throughput (Theoretical Max) Sample Types Sample Handling Up to 320 tests/hour (including ISE) mammalian cell culture; microbial fermentation Up to 90 sample tubes in 6 racks on-board, samples can be replaced during operation Cooled rack position for controls and calibrators Automated sample dilution Barcode reading via laser scanner, with immediate STAT recognition Sample Container Types Sample Volume Sampling tubes: 5 10 ml; 16 100; 16 75; 13 100; 13 75 mm Standard cup: 1.5 ml Micro Micro cup: 650 μl Cup on tube definable Minimal sample volume of 100 µl for several tests, 2 20 µl sample consumption per test Sample Barcode types Code 128, Codabar, Code 2 of 5 interleaved, code 39 Reagents Onboard Capacity Reaction Cells Control Unit System Interfaces Electrical Requirements Physical Dimensions Weight Water Requirements Certification Up to 32 Cedex Bio HT reagent cassettes in 8 racks on-board, 50 800 tests per cassette, Cooled reagent compartment (10 15 C), on-board stability up to 3 months, Calibration typically once per lot Holds 1000 disposable cuvettes with 5 mm path length and 120 240 μl reaction volume HP workstation running Windows XP, Intel Pentium IV with 512 MB RAM, Dual 40 GB hard drives, CD Rom, floppy drive and built-in modem RS 2332 serial interface, bidirectional, built-in modem for remote access 100 125/200 240 volts AC, 50 or 60 Hz, consumption 1200 VA Width: 135 cm (53.1 in.) Depth: 66 cm (25.9 in.) Height: 75 cm (28.5 in.) 320 kg (506 Ibs) Up to 2 liters per hour in operation mode, Type 1 NCCLS CE, UL, C-UL For use in quality control/manufacturing processes only.
System Overview and Capabilities Minimal Hands-on Time: A True Walk-away Analyzer Low maintenance Automated daily maintenance reduces operator intervention Robustness assures minimal downtime Result security Precision Technology Precise temperature controlled rotor ensures reliability Sensitive photometric and turbidmetric measurements Highly sensitive pressure sensors detect incorrect pipetting, even at 2 µl volume Sample probe debris removed upon detection ISE module Rapid measurement of sodium and potassium Flexible change of onboard tests Refrigerated storage for long on-board and calibration curve stability Easy reagent cassette changes while system operates Continuous sample loading Up to 90 samples on-board supported by continuous reloading Automated pre- and post-dilution options
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