Samba Preclin Samba 200. Product Presentation

Samba Preclin Samba 200 Product Presentation

Samba Preclin 420/360 Transducer Ultra-miniature fiber optic pressure sensor no bigger than a grain of salt on a hair! Highly sophisticated, accurate, fast and sensitive instrument designed to measure pressure in gas or liquid. Constructed with patented state-of-the-art micro mechanic and fiber optic technology. A first-class tool for building knowledge and solving problems in numerous application areas. Samba Preclin moves science into new territories! 2

3 Samba Sensors Technology

The Sensor Sensor Low pressure 0.42mm High pressure 0.36mm Optical fiber 0.25mm 4

Measuring Hydrostatic Pressure Space filled with GAS or LIQUID Hydrostatic pressure 5

6 The Sensor on a Finger Tip

X-ray Visibility Radiopaque coating 0.40mm 7

Samba Products

Main Products Ultra-miniature optical pressure transducers in different versions. For preclinical and industrial use. Control units with analog and serial ports. Samba Data Collection software for PC. For research use. Not FDA approved 9

Preclinical Transducers Samba Preclin 420 LP Miniature optical pressure transducers for research in vitro and in vivo. Low pressure range (-50 to +350 mbar). Also with radiopaque coating. 4m long. Samba Preclin 420/360 MR Miniature optical pressure transducers for research in vitro and in vivo. Extra long for use in MRI facilities. Low and high pressure ranges. 10m long. Samba Preclin 360 HP Miniature optical pressure transducers for research in vitro and in vivo. Three high pressure ranges (-0.1 to 5 bar, - 0.1 to 10 bar, -0.1 to 17 bar). Also with radiopaque coating. 4m long. Protective sensor covers are available for special applications 10

Samba Preclin Transducer Pressure can be measured in very small cavities or directly in the tissue of small (or large) animals. The ultra-miniature size minimizes insertion trauma. Insensitive to EM radiation and RF fields (MRI scanner, microwaves etc). Direct measurements with very high precision are made at the sensor location. The transducer does not affect the measuring environment itself. Low risk no electricity or bio-hazardous material. Long transducer possible (up to 10m). 11

Samba 201 Control Unit One fiber-optic port Battery operation Internal memory Absolute or relative measurement Internal barometer 3.2 inch display USB 2.0, analog and RS-232 ports Analogue output: 0-5V Sampling frequency: 1 Hz 40 khz Resolution: 0.1 mbar (~1mm H 2 O) Numerical resolution: 15 bit Numerical res.: 0.1 mbar Operating temp.: 15 35 C Size: 215x110x45mm (8.5x4.3x1.8 in) Weight 950g (2.1 lbs) 12

Samba 202 Control Unit Two fiber-optic ports Battery operation Internal memory Absolute or relative measurement Internal barometer 3.2 inch display USB 2.0, analog and RS-232 ports Analogue output: 0-5V Sampling frequency: 1 Hz 40 khz Resolution: 0.1 mbar (~1mm H2O) Numerical resolution: 15 bit Numerical res.: 0.1 mbar Operating temp.: 15 35 C Size: 215x110x45mm (8.5x4.3x1.8 in) Weight 950g (2.1 lbs) 13

Fast and Simple Start-Up Procedure 1. Insert the Samba transducer The transducer is factory calibrated - No further calibration is needed! 2. Turn on your Samba 200 Control Unit Allow internal circuitry temperature to stabilize (1-2 minutes) 3. Set the zero baseline against ambient air pressure The transducer is factory calibrated - No further calibration is needed! Simply follow instructions on the screen ( ZERO or PREVIOUS ) 4. If needed set measurement rate, Absolute/Relative mode, pressure unit etc. or load pre-defined Profile 5. Connect to PC or Data Acquisition System (DAS) When connected calibrate DAS by pressing ANALOG CALIB. 6. Remove protective cover, apply sensor in object and start measuring! 14

Samba vs. Competition The Samba system 1. has a simple and fast start-up procedure (~3 min) just Plug n Play! 2. is factory calibrated and doesn t need further calibration, only an automatic zero baseline set against the atmospheric pressure. 3. is extremely fast (up to 40 khz data sampling rate) and can record high-speed trauma events, for example ICP events caused by collisions. 4. is fully MRI compatible and completely insensitive to any electromagnetic waves radio waves or electrical noise. Real-time measurements and simultaneous recording of both visual and physiological effects of for example a drug is possible. Gating (MRI scanner triggering) for improved MR image quality and movie sequences is also feasible. 5. can measure pressure both in gas and liquid, which allows a very broad use. 15

Examples of Applications

17 Inserting Transducer into a Mouse Heart

Monitoring Pressure in a Mouse Heart Right Ventricular Pressure also possible! Transducer insertion Am J Physiol Heart Circ Physiol, Vol. 285, pp. H2233-H2239, 2003 18

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Pulse Wave Analysis (PWA) PWA, i.e. the analysis of the pulse pressure wave, is a method for studying vascular stiffness PWA provides valuable information for the characterization of cardiovascular disease, atherosclerosis and endothelial function The analysis is preferably made on a number of pulse wave cycles selected from a specific time period 22

Parameters in Samba PWA Software Pdiast = end-diastolic pressure Psyst = inflection point in systole Paug = max pressure during entire cycle RRint = heart cycle length Tsyst = time between pdiast and psyst PP = paug pdiast Taix = time from pdiast to paug Aug = Paug Psyst AIx (augmentation index) = Aug/(Paug-Pdiast) Further parameters related to the Dicrotic Notch will be available in future software version 23

24 Transducer Introduction into Rat Tail Artery

Triggering the MR Scanner (Gating) 100.00 90.00 Respiration 80.00 Triggering point: Exhalation & min. diastolic blood pressure 70.00 Pressure [mmhg] 60.00 50.00 40.00 30.00 20.00 10.00 Sharper MR images 0.00 & movie sequences! 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 Time [s] B. Bjelke, MD, PhD, Karolinska Institute, Sweden 2003 25

Gating on Samba Signal (IBP) Image of rat heart MRI scanner triggered on rat tail IBP signal Advantages Improved image quality (less artifacts than ECG) Real-time IBP monitoring Volume & CO Movie sequences 26

27 MRI Movie Sequence

Intracranial Monitoring on a Rat Samba transducer 28

ICP in Fluid Percussion Model 3500 3000 Intracranial Pressure Pressure [mbar] 2500 2000 1500 Water Pressure 1000 500 0 0,00 0,10 0,20 Time [s] Acta Neurochir Vol. 147, No. 7, pp. 775-780, 2005 29

Rabbit CNS Trauma Model Eur J Neurosci. Vol. 21, No. 10, pp. 2876-82, 2005 30

Rabbit CNS Trauma Model 1.5 1.4 30000 1.3-20000 1.2 Pressure [bar] 1.1 1 0.9-70000 -120000 Rot. acc.(rad/s^2) 0.8 0.7-170000 0.6 0.5-220000 0.08 0.085 0.09 0.095 0.1 0.105 0.11 Time (s) Occipital lobe pressure transducer Parietotemporal lobe pressure transducer Rotational acceleration Eur J Neurosci. Vol. 21, No. 10, pp. 2876-82, 2005 31

Spinal Disc Measurements Proceedings SPIE 3570, 1998 Samba transducer inserted into disc via a cannula 32

33 Proceedings SPIE 3570, 1998

34 Urodynamic Investigation Transurethral introduction of transducer into bladder

Urodynamic Investigation Transurethral introduction of transducer into bladder Courtesy of Prof. Ulla Sillén, Dept. of Pediatrics Sahlgrenska University Hospital, Sweden 35

Airway Pressure Monitoring Endotracheal tube Samba sensor Ventilator pressure sensor 36

Pressure Measurement in Pediatric ETT Transducer inserted through tube into lower trachea Relative pressure [cmh2o] 30 25 20 15 10 5 0 Samba Respiratory 1 51 101 151 201 251 301 Samples [25 Hz] 37

Intra-folliclular Pressure (during open surgery) Time [sec] P Dahm-Kähler et al. Poster Läkarstämman, 2004 38

Clinical Samba Projects 1. Urodynamic investigations 2. Respiratory pressures 3. Intracranial pressures 4. Intervertebral disc pressure (spinal surgery) 5. Cardiovascular blood pressures 6. Gastro-intestinal pressures 7. Intra-follicle pressure (gynecology) 39

Samba s unique offering High-precision pressure measurements can be performed where never before possible. New science and knowledge can be revealed leading to more publications. APPLICATION AREAS: - Heart and Circulation - Brain and Nervous System - Lungs and Breathing - Gastro-intestinal System - Kidney and Urinary System - Reproduction System - Muscles - Diseases like Cancers, Stroke, Spinal Disorders and more 40

Contact Harvard Apparatus, Ltd Fircroft Way, Edenbridge Kent TN8 6HE England United Kingdom Phone: (44) 1732-864001 Fax: (44) 1732-863356 E-Mail: sales@harvardapparatus.co.uk Internet: www.harvardapparatus.co.uk 41