HPLC Solvets ad mobile phases
CARLO ERBA Reagets A ew reality for laboratory ad idustry CARLO ERBA Reagets, a emiet brad with a wellkow history, operates at a Iteratioal level i Aalytical ad Idustrial Chemistry for laboratory, idustry, o the Italia market, ad eve i Molecular ad Cellular Biology sectors. CARLO ERBA Reagets ows advaced maufacturig plats icludig three busiess premises i Italy, Frace ad Spai.
HPLC Leader o the market of solvets for chromatography ad trace aalysis, CARLO ERBA Reagets exteded its rage of solvets for HPLC i order to satisfy the ever icreasig demad requiremets i terms of equipmet ad detectio methods, particularly for impurities which, by iteractio, ca affect the result s reliability. Our solvets for HPLC meet perfectly the requiremets of this aalytical techique by guarateeig the optimal specificatios o the followig elemets: Purity No volatile residue cotet UV trasmissio CARLO ERBA Reagets Grades RS HPLC Preparative RS HPLC RS HPLC Gradiet RS HPLC Ultra Gradiet / Gold RS HPLC/MS Method of aalysis HPLC HPLC HPLC Preparative Isocratic Gradiet LC-MS I this easy-to-cosult documet, we propose to you a choice of products specially adapted to the preparatio ad aalysis of your samples i HPLC: Solvets ad bleds for LC-MS Solvets for HPLC gradiet Solvets for HPLC isocratic Solvets for preparative HPLC Mobile phases Io pair reagets
Solvets ad bleds for LC-MS LC-MS requires the use of solvets with a miimum impurities which could ifluece the baselie ad thus reduce the aalysis sesitivity. CARLO ERBA Reagets iovates by filterig its solvets for LC-MS at 0.1 μm to satisfy the best purity, optical trasmissio ad o particle criteria. The presece of iorgaic ios ca make the idetificatio ad quatificatio of impurities impossible by formig clusters, givig false peaks, damagig the device. They also have a impact o the service life of the HPLC colums. Water LC-MS Water is ofte used i LC-MS as a solvet or a premixed additive for mobile phases. Mior impurities preset i the water load the LC colums as soo as the aalysis starts ad ca be salted out i gradiet mode. CARLO ERBA Reagets proposes a gradiet quality LC-MS water which ca be used both for UV ad MS detectio. CARLO ERBA Reagets proposes solvets with a very high purity whose specificatios satisfy the requiremets of LC-MS. Reserpie test => tested specifically for a LC-MS applicatio Abscece of a peak greater tha that of the reserpie (609 amu) betwee 50 ad 2 000 amu for a cocetratio of 50 ppb Low metal io cotet (<50 ppb) Purity better tha 99.95 % High UV trasmissio Excellet baselie quality i gradiet, low backgroud oise No volatile residue less tha 2 ppm Filtratio at 0.1 μm Coditioed uder itroge ad a cotrolled atmosphere Quality suitable for use i ULC-MS Zoom of 150 to 2000 m/z of the mass spectrum of acetoitrile 412340 obtaied by TIC aalysis (50 2000 m/z) ESI(+)
Solvets ad bleds for LC-MS LC-MS bleds CARLO ERBA Reagets elarges its offer ad proposes ready-to-use solutios amog the most used mobile phases, which brig you: Produced from LC-MS quality solvets ad specifically tested for LC-MS couplig, these solutios guaratee you : A time savig Test i LC gradiet A precise compositio High UV trasmissio The assurace of a LC-MS quality Solvet purity > 99.95 % Precise additive cotet Low metal io cotet We propose a wide rage of reagets for LC-MS: from «classic» solvets up to the dissolvig of the most used additives i your mobile phases: Product Quality Pkg Code Acetoitrile LC/MS 1 L 412341 2,5 L 412342 Ethyl acetate LC/MS 1 L 448383 Methaol LC/MS 1 L 414831 2,5 L 414832 Propaol-2 LC/MS 1 L 415183 Water LC/MS 1 L 412111 2,5 L 412112 Acetic acid LC/MS 10 x 1 ml 401411 10 x 2,5 ml 401412 50 ml 401413 Formic acid LC/MS 10 x 1 ml 405821 10 x 2,5 ml 405822 50 ml 405823 Trifluoroacetic acid LC/MS 10 x 1 ml 411541 10 x 2,5 ml 411542 50 ml 411543 Acetoitrile + 0.1% v/v formic acid LC/MS 1 L 412331 2,5 L 412332 Acetoitrile + 0.1% v/v trifluoroacetic acid LC/MS 1 L 412321 2,5 L 412322 Water + 0.1% v/v formic acid LC/MS 1 L 412121 2,5 L 412122 Methaol + 0.1% v/v formic acid LC/MS 1 L 414861 2,5 L 414862 Methaol + 0.1% v/v trifluoroacetic acid LC/MS 1 L 414871 2,5 L 414872
Solvets for HPLC gradiet We propose several rages for the gradiet mode aalysis: Gold Ultragradiet, Plus Gradiet. The gradiet cotrol of elutio ad drift AT with critical wavelegths of our HPLC solvets Gold ad Plus guaratee a peak free baselie. Their optimal sesitivity allows you to evaluate i the best possible way the impurities of your samples. To make sure that o particle i the mobile phase will hider your aalyses, we carry out a microfiltratio of our GOLD solvets at 0.1 μm ad for HPLC Gradiet Plus at 0.2 μm. The GOLD quality is adapted to the aalysis by UPLC-MS. Product Quality Pkg Code Acetoitrile HPLC - Gold 1 L 412371000 2,5 L 412372000 5 L 412375 HPLC Plus Gradiet ACS-Reag.Ph.Eur.-Reag.USP 1 L 412391000 2,5 L 412392000 Water HPLC Plus 1 L 412141 2,5 L 412142 Ethaol HPLC Plus 1 L 412701 2,5 L 412702 Isopropaol HPLC Plus 1 L 412711000 2,5 L 412712000 Methaol HPLC - Gold 2,5 L 412722 5 L 412725 HPLC Plus Gradiet 1 L 412381 2,5 L 412383
Solvets for HPLC gradiet Comparative table Specificatios Characteristics Uits Acetoitrile Methaol LC MS GOLD HPLC Plus GOLD HPLC Plus Ultragradiet Gradiet LC MS Ultragradiet Gradiet Purity (GC) % mi 99.95 mi 99.9 mi 99.9 mi 99.95 mi 99.9 mi 99.9 Water cotet % max 0,01 max 0,01 max 0,01 max 0.02 max 0.02 max 0.02 No volatil residue ppm max 2 max 2 max 2 max 2 max 5 max 5 Acidity meq/g max 0,0005 max 0,0003 max 0,0008 max 0,0003 max 0,0003 max 0,0005 Titrable base meq/g max 0,0002 max 0,0002 max 0,0002 max 0,00006 max 0,00006 max 0,0002 Absorbace at 190 m A.U. max 0,6 max 1,00 at 200 m A.U. max 0,03 max 0,05 at 220 m A.U. max 0,007 max 0,05 at 254 m A.U. max 0,005 max 0,01 Trasmissio UV (1cm - Réf : eau) at 195 m % mi 80 mi 80 mi 79 at 197 m % at 200 m % mi 95 mi 95 mi 90 at 210 m % mi 95 mi 30 mi 30 mi 30 at 220 m % mi 98 mi 98 mi 55 mi 50 at 225 m % mi 99 mi 65 mi 65 from 230 m % mi 99 mi 99 at 235 m % mi 85 mi 85 mi 80 at 240 m % mi 90 at 250 m % mi 95 mi 95 From 255 % mi 98 from 260 m % mi 98 mi 98 mi 98 Fluorescece (quiie) at 254 m ppb max 1 max 1 max 1 max 1 max 1 max 1 Fluorescece(quiie) a 365 m ppb max 0.5 max 0.5 max 0.5 max 1 max 1 max 1 Fluorescece (quiie) at 450 m ppb max 0.5 max 0.5 HPLC gradiet test (1) - Coform Coform Coform Coform Coform Coform HPLC gradiet (1) at 210 m mau max 1 max 1 max 2 at 235 m mau max 2 max 2 max 2 at 254 m mau max 0.2 max 0.2 max 0,8 max 1 max 1 max 1 Drift at 210 m mau max 12 (1) Gradiet test : biary gradiet H2O/acetoitrile from 5 to 90% i acetoitrile for 16 miutes at 1.8 ml/mi LC/MS test TIC ( 50-2000 m/z) ESI (+) Sesitive impurities (i reserpie) ppb max 50 max 50 Metal cotet (2) : Alumiium ppb max 50 max 50 Iro ppb max 50 max 50 Sodium ppb max 50 max 50 Calcium ppb max 50 max 50 Magesium ppb max 50 max 50 Potassium ppb max 50 max 50 (2) Cocetratios measured at batch release
Solvets for HPLC isocratic Our solvets for HPLC combie chemical purity with optimal optical trasmissio. Available i glass bottles (1L ad 2.5L) or stailess steel shuttle drums (5 to 1000L), their characteristics satisfy the requiremets of the most advaced HPLC techiques. Product Pkg Code Acetic Acid 1 L 401431 2,5 L 401432 Acetoe 1 L 412501 2,5 L 412502 Acetoitrile 1 L 412411000 2,5 L 412412000 Butaol 1 L 412511000 1-Chlorobutae 1 L 431821 Chloroform stabilized with amylee 1 L 412571 2,5 L 412572 Chloroform stabilized with ethaol 1 L 412652 2,5 L 412653 Cyclohexae 1 L 412431000 2,5 L 412432000 Dichloromethae stabilized with amylee 1 L 412621000 2,5 L 412622000 Dichloromethae stabilized with ethaol 1 L 412662 2,5 L 412661 Ethaol absolute ahydrous 2,5 L 412522 Ethyl ether 1 L 412671 2,5 L 412672 Ethyle acetate 1 L 412611000 2,5 L 412612000 -Heptae 1 L 412591000 2,5 L 412592000 -Hexae 1 L 412601000 2,5 L 412602000 Hexae 99 % 1 L 412691 2,5 L 412692 Isohexae 2,5 L 445151 Isooctae 1 L 412441000 2,5 L 412442000 Methaol 1 L 412533 2,5 L 412532 Propaol-1 2,5 L 412542000 Propaol-2 1 L 412421000 2,5 L 412422000 Ter-butylmethylether 1 L 432031 2,5 L 432032 Tetrahydrofura 1 L 412451000 2,5 L 412452000 Toluee 1 L 412641000 2,5 L 412642000 Triethyl Ami 1 L 489631 2,5 L 489633
Solvets for preparative HPLC Our rage of solvets for HPLC preparative have bee desiged to satisfy the requiremets of separatios ad purificatios. Their low o volatile residue cotet (from 5 to 10 ppm maximum) allows optimizig the operatio coditios ad to make impurity-free preparatios. All these solvets are available i 2.5 L bottles ad i stailess steel shuttle drums from 5 to 1 000 L. Product Code 2,5L Acetoitrile 412409 Chloroform (stabilized with ethaol) 438641 Dichloromethae (stabilized with amylee) 463281 Dichloromethae (stabilized with ethaol) 463291 Ethyl acetate 448211 Propaol-2 415112 Tetrahydrofura 487352 Toluee 488531 Ter-butylmethylether 432022000 All our solvets for HPLC ad eluet phases ca be delivered i adapted packagigs: glass bottles of 1 L or 2.5 L, or i stailess steel shuttle drums from 5, 10, 25, 200 litres with samplig systems adapted to your eeds. (special documetatio available o request)
Mobile phases If you regularly use a eluet phase, we ca prepare it for you accordig to your specificatios. Your mobile phase is prepared from HPLC quality solvets accordig to procedures (respect of the GMPs) ad with validated equipmet i compliace with the pharmacopeia. The ready-to-use eluet phase esures you: Importat time savig for preparatio Reductio of risks liked to the hadlig of toxic or hazardous products Guarateed ph Possibility of large sized homogeeous batches Labelig coformig to legislatio ad to BPLs It is supplied with: Certificate of aalysis of the batch Safety data sheet Examples Acetoitrile + methaol + buffered ph 5 Acetoitrile + water Ethyl acetate + toluee Water + TFA Water + THF Bacth umber of the mix Expiry date Compositio Specificatios defied by customer Date
Io pair reagets The separatio of ioic ad highly polar substaces o reversed phases HPLC colums is facilitated by io pair reagets. Their purity ad UV absorptio test are very importat as mobile phase additives. CARLO ERBA Reagets proposes a rage of most used io pair reagets. Product Code Sodium 1-Heptaesulfoate 25 g 405851 Sodium 1-hexaesulfoate 25 g 405621 Sodium 1-octaesulphoate 25 g 405861 Sodium 1-petaesulphoate 25 g 405841 Sodium dodecylbezeesulfoate 10 g 405351 Sodium p-tolueesulfoate 100 g 411504 Tetrabutylammoium hydrogesulfate 250 g 487101 Trimethylcetylammoium bromide 50 g 489833
HPLC CER HPLC 2013/01 ALL PICTURES AND SPECIFICATIONS ARE PURELY INDICATIVE AND MAY VARY WITHOUT NOTICE. WWW.CARLOERBAREAGENTS.COM ITALY CARLO ERBA Reagets S.r.l. VIA R. MERENDI, 22 20010 CORNAREDO (MI) TEL. +39 02 93 99 190 FAX +39 02 93 991 001 FRANCE CARLO ERBA Reagets SAS CHAUSSÉE DU VEXIN 27 106 VAL DE REUIL TEL. +33 2 32 09 20 00 FAX +33 2 32 59 11 89 SPAIN CARLO ERBA Reagets S.A. CALLE FILADORS 08 208 SABADELL TEL. +34 93 693 37 35 FAX +34 93 724 31 68