Artificial Insemination PRODUCERS can choose to use natural or artificial means of breeding their animals. Technology has advanced in the last 30 to 40 years to allow animal producers to use means other than natural mating of the male and the female. Artificial means have their advantages and disadvantages. These must be weighed before deciding whether to use the techniques in an animal breeding system. Objectives: 1. Explain the process of artificial insemination. 2. Identify the advantages and disadvantages of artificial insemination. Key Terms: artificial insemination estrus detection sexed semen standing heat The Process of Artificial Insemination Artificial insemination (AI) is the act of collecting semen from the male, preserving it until use, and depositing it in the reproductive tract of the female when she expresses estrus. This process is used for many different reasons and has become quite popular in the animal production industry. It is commonly used with beef, dairy, swine, horses, and fish. Also, it is used sparingly with dogs, sheep, and wildlife. Research continues into the development of its use with other animal species. The techniques and technology of artificial insemination have advanced and been altered for ultimate efficiency. Page 1 www.mycaert.com
SEMEN COLLECTION For artificial insemination to be used, semen has to be collected from males. It is collected in different ways, depending on the species. Semen can be collected using electronic stimulation, causing an unaroused ejaculation. It can be collected with the use of an artificial vagina. When this procedure is used, the male typically has to be aroused by a teaser animal. Manual or hand collecting is also used with some species. It involves allowing the male to mount a dummy animal, erect its penis, and expel semen. When using the artificial vagina or manual collection technique, it is best to allow the animal to become as aroused as possible before it ejaculates. This permits collection of the best samples. Once the semen is collected, it is evaluated for quality, diluted, mixed with an extender, and cooled for storage. The semen is diluted and mixed with an extender to allow for more units of product from one collection without sacrificing quality to conceive a pregnancy. With some species, the semen can be stored in a refrigerator for three to five days without losing any great amount of quality. With other species, it is slowly cooled to 320 F ( 195.6 C) using liquid nitrogen. If properly maintained, the semen will stay viable for 30 to 50 years, possibly longer. SEXED SEMEN One of the newest techniques in the artificial insemination industry is sorting semen by sex. Sexed semen is semen in which the male and female chromosomes have been sorted so that when the semen is used, a male or female offspring will be conceived. The technique has proven to be 90 to 98 percent accurate and is very beneficial for breeding some species. For example, in the dairy industry, heifer calves are much more valuable for replacements than bull calves. This is especially important for breeding cows with extremely high milk production. Sexed semen is collected in the same manner as nonsexed semen. After collection, it is sorted using a highly sophisticated machine that can determine the difference between chromosomes. ESTRUS SYNCHRONIZATION Animal producers have found it most beneficial to use artificial insemination when multiple animals are in estrus at the same time. Much research has been dedicated to synchronizing estrus of a group of animals. Estrus synchronization is the act of manipulating the estrous cycle of animals to stimulate them into FIGURE 1. Producers regularly use artificial insemination in the dairy industry. (Courtesy, USDA) Page 2 www.mycaert.com
experiencing estrus at a predetermined time. This allows the producer to have all equipment prepared when the animals are ready to breed as well as when they are ready to give birth. Basically, the breeding and birthing season is shortened, allowing for better labor utilization and uniform offspring size and age. To manipulate the estrous cycle, a producer injects natural and artificially created hormones into an animal or feeds them to the animal. The hormones stay in the body only a short time and have no effect on meat or animal safety. ESTRUS DETECTION To achieve high conception rates with artificial insemination, producers must become proficient in recognizing estrus and secondary signs of estrus. The most common indicator is a female standing to be mounted. This is called a standing heat because the animal is allowing another animal to mount her in a manner that would facilitate natural breeding. Secondary signs of estrus include mucus discharge from the vagina, swollen or reddish color of the vulva, licking of the coat or loving-like manners toward other females, and continued vocalization. Estrus detection requires checking the animals several times throughout the day, for extended periods. Technology can also be used to detect estrus. Patches can be placed on the rump of a female to record whether the animal has been mounted, and blood can be tested for hormone levels. Both these procedures are very expensive and are not always practical for every situation. INSEMINATION The final step in artificial insemination is depositing the semen into the female, or performing insemination. The timing and the exact placement of the semen vary by species. In general, the semen needs to be appropriately prepared for deposit to maximize conception. If frozen, it HELP WANTED ON THE JOB CAREER CONNECTION: Artificial Insemination Technician Many technicians are trained each year to inseminate animals. Typically, an inseminator specializes in one species but is not necessarily restricted to one if properly trained. Inseminating animals can be either a full-time or part-time career, depending on the area in which the technician works. Individuals must like to work with animals, be willing to work long hours during the prime breeding season, and be flexible with travel. To become a technician requires many hours of training, followed by several weeks or months of skill development. To get the proper training, an individual will need to attend an organized school for artificial insemination, then have access to many animals in estrus to develop the proper skills. Compensation for an inseminator varies from $5 to $30 per animal or up to $300 or $400 a day. Page 3 www.mycaert.com
Cervix Rectum Catheter Vulva Pelvic bone Ovaries Uterine horns Bladder FIGURE 2. Cows are artificially inseminated by palpating the animal through the rectum and passing the insemination tool through the cervix. should be thawed at 98 F (36.7 C) for not less than 1 minute or more than 10 minutes. The semen is deposited in the female by using an artificial insemination gun or catheter. The tool is passed into the reproductive tract to the point where the semen should be deposited. In some species, such as the cow and the horse, the animal must be palpated with an arm to facilitate insemination. The procedure is fairly fast. Substantial training is needed to gain proficiency. Artificial insemination is not recommended for individuals who have had little to no professional instruction. Advantages and Disadvantages of Artificial Insemination Artificial insemination has its advantages and disadvantages. Animal producers should carefully evaluate the positives and negatives before fully implementing AI as their breeding system. Without exploring all possibilities and aspects involved with the process, a producer could cause severe damage to his or her animal production system. ADVANTAGES Some producers will praise artificial insemination to the point that it appears to be the only method to use. That is because it does possess some real advantages. Page 4 www.mycaert.com
Superior males or sires can be utilized across a greater number of animals. Semen can be shipped all over the world at minimal cost, allowing one male to service thousands of females each year. After each collection, semen can be divided into 30 to 50 units, whereas if the male naturally serviced the female, only 1 unit would be utilized. Artificial insemination also eliminates the danger of housing males. Males can be extremely aggressive during the breeding season, causing injury or death. Without the need to house or care for males, costs are reduced. Costs of obtaining units of semen are much less than those associated with the original investment in a male, lifetime management of the animal, and regular care. Also, several different sires can be utilized in one group of animals. Each female can be matched and bred to the most suitable male to achieve desired results. For example, a female might have a high-quality trait in one area but be lacking in another. She could be mated with a male that corrects her faults and still capitalize on her high-quality traits. DISADVANTAGES Other producers will be very discriminating toward artificial insemination to the extent that it would appear to be a worthless procedure. That is because it does have its disadvantages. Artificial insemination requires the skill and training of a technician. In large production operations, several individuals may need to be trained to service females. Training and skill development can be very time consuming and result in additional costs, including compensation of the trainer. The original investment in equipment can also be quite costly. AI requires several expensive specialty tools that must be maintained on a regular basis. Producers using frozen semen must have nitrogen added to their tanks every 30 to 60 days at a charge from the distribution company. Synchronization can also be expensive when used in a large group of animals. The hormones involved can cost as much as $6 to $14 per animal. Also, the animals must be restrained for injections and insemination and must be handled in a way that prevents injury to them and to people working around them. To achieve high conception rates, management must be increased. Not only does estrus detection have to occur several times throughout the day, but careful records must be kept to prevent accidental matings and mistakes. Summary: Artificial insemination (AI) is the act of collecting semen from the male, preserving it until use, and depositing it in the reproductive tract of the female when she expresses estrus. The semen may be sexed if desired. Much research has gone into estrus synchronization, the stimulating of a group of females to experience estrus at a predetermined time. Producers must become proficient in recognizing estrus and secondary signs of estrus. Page 5 www.mycaert.com
AI has its advantages and disadvantages. It allows for better utilization of sires, reduces the hazards and costs of keeping males, and allows for the utilization of multiple sires. However, it requires extensive training and skill development, has a high initial investment, has maintenance costs, and increases requirements for management. Animal producers should weigh the positives and the negatives to determine if AI is appropriate for their production systems. Checking Your Knowledge: 1. What is artificial insemination? 2. Why might an animal producer want to use sexed semen in artificial insemination? 3. How does estrus synchronization benefit an animal producer? 4. What are some signs of estrus? 5. List the advantages and disadvantages of artificial insemination. Expanding Your Knowledge: Take a field trip to the facility of an animal producer in your location who is utilizing artificial insemination. Ask to view all parts of the AI process that take place there. These could include semen collection, processing, and storage and the insemination of animals. Ask why the producer uses or does not use estrus synchronization. Be sure to explore the advantages and disadvantages the producer is receiving from using AI. Web Links: Artificial Insemination http://www.showdogsupersite.com/kenlclub/breedvet/nvai.html Semen Collection http://www.equine-reproduction.com/articles/collection.htm Successful Insemination Through Estrus Synchronization http://msucares.com/pubs/publications/p2207.htm Agricultural Career Profiles http://www.mycaert.com/career-profiles Page 6 www.mycaert.com