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12 th INTERNATIONAL SYMPOSIUM ON STEM CELL THERAPY CARDIOVASCULAR INNOVATIONS & www.cardiovascularcelltherapy.com Symposium Director: Francisco Fernández Avilés PRELIMINARY SCIENTIFIC AGENDA MADRID. SPAIN, MAY 28 th - 29 th 2015 AULA MAGNA. PABELLÓN DOCENTE. HOSPITAL GENERAL UNIVERSITARIO GREGORIO MARAÑÓN. C/ IBIZA, 43. 28007 MADRID

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12 th INTERNATIONAL & SYMPOSIUM ON STEM CELL THERAPY CARDIOVASCULAR INNOVATIONS www.cardiovascularcelltherapy.com 1 SCIENTIFIC AGENDA 2 ORGANIZATION 3 EDUCATIONAL OBJECTIVES 4 ACCREDITATION 5 GENERAL INFORMATION 6 WEBSITE 7 FACULTY 8 COLLABORATION AND SPONSORSHIP www.cardiovascularcelltherapy.com 3

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THURSDAY, 28 th May 2015 SCIENTIFIC AGENDA 1 7.45 8.30 REGISTRATION 8.30 9.00 WELCOMING 9.00 10.00 OPENING LECTURES CHALLENGES IN REGENERATIVE CARDIOVASCULAR MEDICINE Chairs: Andreas Zeiher (Frankfurt, Germany) John Martin (London, UK) 9.00-9.20 Learning from development Nadia Rosenthal (Melbourne, Australia) 9.20-9.40 Challenging targets for cardiac stem cell therapy. The cell, the niche, anything else? André Terzic (Rochester, USA) 9.40-10.00 Is there a role for cardiac regeneration in advanced heart disease? Overcoming frontiers to move forward. Valentin Fuster (New York, USA) 10.00 10.30 Coffee Break and Poster Exhibition 10.30 12.00 CARDIOVASCULAR REGENERATIVE MEDICINE. CRISIS, WHAT CRISIS? Chairs: José Luís de la Serna (El Mundo Salud) (Madrid, Spain) Cristina Garmendia (Genetrix) (Madrid, Spain) Panel Discussants: Timothy Henry Dominique Charron Stefanie Dimmeler André Terzic (Los Angeles, USA) (Paris, France) (Frankfurt, Germany) (Rochester, USA) 10.30 (12 ) Importance of the cells. What are we using? Piero Anversa (Boston, USA) 10.42 (12 ) Can we improve the regenerative power of transplanted or native cells? Proteins, genes, what else? Atta Behfar (Rochester, USA) 10.54 (12 ) Local milieu in cardiac regeneration. Barriers and opportunities in acute and chronic settings Philippe Menasché (Paris, France) 11.06 (12 ) Lights and shadows of clinical trials. Should we continue? In which setting? How? Bernard Gersh (Rochester, USA) 11.18 (12 ) Cell based therapies to prevent and cure heart failure. More than an adventure? Douglas W. Losordo (Boston, USA) 11.30 (12 ) Cardiac regenerative medicine beyond scientists and clinicians. Are we doing well? John Martin (London, UK) 11.42 12.00 Panel Discussion 5

THURSDAY, 28 th May 2015 12.00 12.45 LUNCH AND POSTER EXHIBITION Chairs and jury: Philippe Menasche (Paris, France) Felipe Prosper (Pamplona, Spain) In this Poster Exhibition, research teams involved in the topic of the Symposium will have the opportunity of presenting selected parts of their work to the audience. The Poster Review Committee will select which the best posters of the session are. At the end of the session, the verdict will be communicated. The winners of the Posters Awards will have to present their work (oral presentation) the following day in the morning, in the session named Best Poster Presentation, when they will receive their prize. For more information regarding Poster Exhibition and Poster Awards, please contact us via e-mail at posters@cardiovascularcelltherapy.com 12.45 14.45 WHAT S NEW IN STEM CELL THERAPY FOR CARDIAC REPAIR? Chairs : Timothy Henry (Los Angeles, USA) André Terzic (Rochester, USA) Panel Discussants: Roberto Fdez-Viña Kai C. Wollert Stefan Janssens Jerome Roncalli 12.45 (12 ) Cardiac valve repair. Beyond myocardium. Amir Lerman 12.57 (12 ) What s new in induced pluripotent stem cells. Eric A. Adler 13.09 (12 ) Combinations of stem cells. Which ones to choose? Joshua Hare (Buenos Aires, Argentina) (Germany) (Leuven, Germany) (Toulouse, France) (Rochester, USA) (San Diego, USA) (Miami, USA) 13.21 (12 ) Role of cardiopoiesis in the second-generation stem cells era. Almudena Martínez-Fernández (Rochester, USA) 13.33 (12 ) Cell based therapies: Novel developments in the arena of delivery strategy Steven Chamuleau (Utrecht, The Netherlands) 13.45 (12 ) Stemless biological therapies for cardiac repair. Stefanie Dimmeler 13.57 (12 ) Latest clinical news: the Dream-HF experience Emerson Perin 14.09 (12 ) Future clinical news: Trials in perspective Thomas J. Povsic (Frankfurt, Germany) (Houston, USA) (Durham, USA) 14.21 14.45 Panel Discussion 6

SCIENTIFIC AGENDA 1 14.45 15.15 Coffee break and poster exhibition 15.15 15.25 Posters Award - Winners communication Chairs: Philippe Menasché (Paris, France) Felipe Prosper (Pamplona, Spain) 15.25 17.35 THIS YEAR WE DEBATE Chairs: Bernard Gesh (Rochester, USA) Pedro Luís Sánchez (Salamanca, Spain) Panel discussants: Andreas Zeiher Timothy Henry Philippe Menasché Andreu Climent André Terzic (Frankfurt, Germany) (Los Angeles, USA) (Paris, France) (Madrid, Spain) (Rochester, USA) 15.25-16.16 The Clinical Debate: Why metanalyses are contradictory 15.25 (12 ) Meta-analyses and systematic reviews results on hold. Factual discrepancies. Alexandra N. Nowbar (London, UK) 15.37 (12 ) How individual patient data change messages. The ACCRUE experience Maryann Gyongyosi (Vienna, Austria) 15.49 (12 ) Meta-analyses and systematic reviews results are valid and still offering evidences. Enca Martí Rendon (Oxford, UK) 16.01-16.16 Discussion 16.16-16.55 How to improve: the cardiac stem cells debate 16.16 (12 ) Native and enhanced CSC are the real workhorse cells. Mark A. Sussman. (San Diego, USA) 16.28 (12 ) Do we really know what CSC are and how to use them? Bernardo Nadal-Ginard (London, UK) 16.40-16.55 Discussion 16.55-17.34 How to improve: The bioengineering debate: matrixes and scaffolds. 16.55 (12 ) Bioartificial matrixes. Available and versatile. Felipe Prosper (Pamplona, Spain) 17.07 (12 ) Cadaveric matrixes. Native myocardial architecture is mandatory. Doris Taylor (Houston, USA) 17.19-17.34 Discussion 7

Notes 8

FRIDAY, 29 th May 2015 SCIENTIFIC AGENDA 1 9.00 9.30 BEST POSTERS PRESENTATION Chairs: Philippe Menasche (Paris, France) Felipe Prosper (Pamplona, Spain) 9.30 10.30 RECENT CONTRIBUTIONS PRESENTED BY YOUNG INVESTIGATORS Chairs: Philippe Menasche (Paris, France) Felipe Prosper (Pamplona, Spain) Panel discussants: David G. Dorado Javier Bermejo Joshua Hare Stefan Janssens (Barcelona, Spain) (Madrid, Spain) (Miami, USA) (Leuven, Belgium) 9.30 9.40 Cardioprotection by telomerase treatment Christian Bar (Maria Blasco s group. Madrid, Spain) 9.40 9.50 Natura killer cell crosstalk with allogenic human cardiac-derived stem/progenitor cells control persistence Reem Al Daccak (Dominique Charron s group. Paris, France) 9.50 10.00 Myocardial infarct scar is formed by interacting resident epicardial-derived fibroblastsand recruited bone marrow cells Adrian Ruiz Villalba (Felipe Prosper s group. Pamplona, Spain) 10.00 10.10 Novel CMR based techniques to detect cardiac fibrosis Johannes M.I.H. Gho (Steven Chamuleau s group. Utrecht, The Netherlands) 10.10 10.30 Panel Discussion 10.30-11.00 Coffee Break 9

FRIDAY, 29 th May 2015 11.00 12.50 DISCOVERIES AND INVENTIONS Chairs: Dominique Charron (Paris, France) Alberto San Román (Valladolid, Spain) Panel discussants: Juan Carlos Chachques Raquel Yotti Angel Arenal Giulio Pompilio (Paris, France) (Madrid, Spain) (Madrid, Spain) (Milan, Italy) 11.00 11.45 Genes and cells to fight against pulmonary hypertension. Duncan Stewart (Otawa, Canada) 11.45 12.00 High-density optical mapping in a monolayer of ipsc-derived cardiomyocytes as a tool for understanding arrhythmic disorders. Andreu M. Climent (Madrid, Spain) 12.00 12.15 Patient-specific stem cell manufacturing for the development of dedicated cardiovascular drugs. Joseph Wu (Los Angeles, USA) 12.15 12.30 Bioprosthetic Carmat Total Artificial Heart: from a dream to the first-in-human implantation. Christian Latremouille (Paris, France) 12.30 12.50 Panel Discussion 12.50 13.30 Break 13.30 13.50 SPECIAL LECTURE Chairs: Rafael Matesanz (Madrid, Spain) Arturo Fdez-Cruz (Madrid, Spain) Interspecies genetic manipulation for organ manufacturing. What about the heart? Juan Carlos Izpisua (La Jolla, USA) 13.50 14.20 CLOSING SESSION. SECOND MADRID CARDIAC REGENERATION AWARDS Chairs: John Martin (London, UK) Rafael Matesanz (Madrid, Spain) 14.20 ADJOURN Francisco Fernández-Avilés (Madrid, Spain) 10

11 SCIENTIFIC AGENDA 1

Notes 12

ORGANIZATION 2 DIRECTION Francisco Fernández-Avilés Servicio de Cardiología Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM) Hospital General Universitario Gregorio Marañón (Madrid, Spain) CO-DIRECTION Mª Eugenia Fernández Santos Instituto de Investigación Sanitaria Gregorio Marañón Hospital General Universitario Gregorio Marañón (Madrid, Spain) Bernard Gersh Mayo Clinic (Rochester, USA) John F. Martin Centre for Cardiovascular Biology & Medicine (London, United Kingdom) José Alberto San Román Hospital Clínico-Universitario de Valladolid (Valladolid, Spain) Pedro L. Sánchez Hospital Clínico Universitario de Salamanca (Salamanca, Spain) Ricardo Sanz Ruiz Instituto de Investigación Sanitaria Gregorio Marañón Hospital General Universitario Gregorio Marañón (Madrid, Spain) Doris A. Taylor Texas Heart Institute (Houston, USA) Andreas W. Zeiher University of Frankfurt (Frankfurt, Germany) 13

TECHNICAL DIRECTION Ana Fernández Baza Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM) Servicio de Cardiología Hospital General Universitario Gregorio Marañón INTERNATIONAL SCIENTIFIC ADVISORY BOARD Jozef Bartunek (Aalst, Belgium) Nabil Dib (San Diego, USA) Stefanie Dimmeler (Frankfurt, Germany) Valentín Fuster (New York, USA) Bernard Gersh (Rochester, USA) Juan Carlos Izpisúa (La Jolla, USA) Stefan Janssens (Leuven, Belgium) Anthony Mathur (London, United Kingdom) John Martin (London, United Kigdom) Philippe Menasche (Paris, France) Bernardo Nadal-Ginard (London UK) Emerson Perin (Houston, USA) Doris Taylor (Houston,USA) William Wijns (Aalst, Belgium) James T. Willerson (Houston, USA) EXECUTIVE LOCAL COMMITTEE Lucía Alvaredo Olmos Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM) - Hospital G. Universitario Gregorio Marañon, Madrid Carmen Glez. Morante Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM) - Hospital G. Universitario Gregorio Marañon, Madrid Enrique Gutiérrez Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM) - Hospital G. Universitario Gregorio Marañon, Madrid Mª Eugenia Vázquez Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM) - Hospital G. Universitario Gregorio Marañon, Madrid Adolfo Villa Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM) - Hospital G. Universitario Gregorio Marañon, Madrid ORGANIZATION Instituto de Investigación Sanitaria Hospital Gregorio Marañón. Servicio de Cardiología. Hospital General Universitario Gregorio Marañón, Madrid. Red Temática de Investigación Cardiovascular del Instituto de Salud Carlos III (RIC) Red Temática de Terapia Celular del Instituto de Salud Carlos III (TERCEL) Grupo de Terapia Celular Aplicada al Miocardio (TECAM, Hospital General Universitario Gregorio Marañón -Madrid-, Hospital Clínico-Universitario de Valladolid, Hospital Río Hortega - Valladolid-, Instituto de Biología y Genética Molecular - Universidad de Valladolid-) Task Force on Stem Cell Regeneration of the Heart (European Society of Cardiology) 14

EDUCATIONAL OBJECTIVES 3 This symposium is aimed at both medical and non-medical scientists interested in the study and application of stem cell therapy and other biotechnological advances for the prevention and treatment of cardiovascular failure. For this reason the audience comprises: a. Preclinical researchers from all areas wishing to deepen their knowledge of cardiovascular biotechnological developments with therapeutic potential. b. Doctors specialising in the care of patients suffering from cardiovascular diseases, and who are interested in finding out and discussing the current and the future prospects of these new therapeutic methods c. Interventional cardiologists and cardiac surgeons interested in the results and perspectives of: gene vehiculization techniques, stimulation of endogenous progenitors, cell transplantation delivery systems or electromechanical devices to fight against cardiovascular diseases. d. Medical or no medical professionals interested in the development of units and networks aimed at the translation of cardiovascular knowledge from bench to bedside and vice versa. e. Medical or no medical professionals interested in envisaging the opportunities for competition of biotechnology in the cardiovascular field At the end of the conference the attendees will have acquired the following skills: 1. Knowledge and ability to discuss the role of in the role of progenitor cells in the integrity of the cardiovascular system of a healthy individual. 2. Knowledge and ability to discuss the latest experimental advances on cardiovascular repair, through adult or embryonic stem cell transplantation, or by stimulation of endogenous progenitors 3. Knowledge and ability to objectively debate the current state of feasibility, safety and efficacy with regards to the clinical application of cell therapy and other new biotechnological treatments. 4. Knowledge and ability to debate objectively on the efficiency of these types of treatment in patients with acute or chronic cardiovascular diseases. 5. Ability to predict the possibilities of scientific and professional attitude with regards to offering these therapeutic methods. 6. Ability to evaluate and decide one s own ability to participate in the application of these therapies for healthcare, clinical, and preclinical purposes. 7. Ability to identify those institutions and groups with the highest performance capabilities in these fields, and to establish contacts for educational, scientific, and healthcare purposes. 15

ACCREDITATION 4 Spanish Society of Cardiology. Continuing Medical Education Accreditation Program. EBAC (European Board for Accreditation in Cardiology). Activity accredited by the European Board for Accreditation in Cardiology Comisión de Formación Continuada. Sistema Nacional de Salud.. 16

GENERAL INFORMATION 5 MEETING VENUE Aula Magna. Pabellón Docente. Hospital General Universitario Gregorio Marañón. C/ Ibiza, 43. 28007 Madrid, Spain RETIRO DATE May 28 th and 29 th, 2015 OFFICIAL LANGUAGES English and Spanish (simultaneous translation will be available) TECHNICAL SECRETARIAT Viajes Orbiazul C/ Alonso Pesquera, 2 47002 Valladolid Tf. 00 34 983 30 91 92 Fx. 00 34 983 29 53 51 housing@cardiovascularcelltherapy.com info@cardiovascularcelltherapy.com 17

WEBSITE 6 The website www.cardiovascularcelltherapy.com is a virtual place which contains all the information related to this Symposium and its previous editions It is possible through this web-site to access the scientific program, to register and to reserve accommodation. It is also a priority for this web site to offer updated information about Stem Cell Therapy for Cardiovascular Diseases, so appropriate access to related sites has been created. You can also join us on Facebook and Twitter. @cardiostemcell #TECAM2015 18

FACULTY 7 Eric A. Adler (San Diego, USA) Reem Al Daccak (Paris, France) Piero Anversa (Boston, USA) Angel Arenal (Madrid, Spain) Christian Bar (Madrid, Spain) Atta Behfar (Rochester, USA) Javier Bermejo (Madrid, Spain) Juan Carlos Chachques (Paris, France) Steven Chamuleau (Utrecht, The Netherlands) Dominique Charron (Paris, France) Andreu M. Climent (Madrid, Spain) Stefanie Dimmeler (Frankfurt, Germany) David G. Dorado (Barcelona, Spain) Francisco Fdez-Aviles (Madrid, Spain) Arturo Fdez-Cruz (Madrid, Spain) Roberto Fdez-Viña (Buenos Aires, Argentina) Valentin Fuster (New York, USA) Cristina Garmendia (Madrid, Spain) Rafael Matesanz (Madrid, Spain) Philippe Menasché (Paris, France) Bernardo Nadal-Ginard (London, UK) Alexandra N. Nowbar (London, UK) Giulio Pompilio (Milan, Italy) Felipe Prosper (Pamplona, Spain) Jerome Roncalli (Toulouse, France) Nadia Rosenthal (Melbourne, Australia) Alberto San Román (Valladolid, Spain) José Luís Serna (Madrid, Spain) Duncan Stewart (Otawa, Canada) Mark A. Sussman (San Diego, USA) Doris Taylor (Houston, USA) André Terzic (Rochester, USA) Kai C. Wollert (Germany) Joseph Wu (Los Angeles, USA) Raquel Yotti (Madrid, Spain) Andreas Zeiher (Frankfurt, Germany) Bernard Gersh (Rochester, USA) Mariann Gyongyosi (Viena, Austria) Joshua Hare (Miami, USA) Timothy Henry (Los Angeles, USA) Juan Carlos Izpisua (La Jolla, USA) Stefan Janssens (Leuven, Belgium) Christian Latremouille (Paris, France) Amir Lerman (Rochester, USA) Douglas W. Losordo (Boston, USA) Johannes M.I.H. Gho (Utrecht, The Netherlands) Enca Martí Rendon (Oxford, UK) John Martin (London, UK) Almudena Martínez-Fernández (Rochester, USA) 19

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DIRECTION Francisco Fernández-Avilés Instituto de Investigación Sanitaria Gregorio Marañón Hospital General Universitario Gregorio Marañón (Madrid, Spain) CO-DIRECTION Mª Eugenia Fernández Santos Instituto de Investigación Sanitaria Gregorio Marañón Hospital General Universitario Gregorio Marañón (Madrid, Spain) Bernard Gersh Mayo Clinic (Rochester, USA) John F. Martin Centre for Cardiovascular Biology & Medicine (London, United Kingdom) José Alberto San Román Hospital Clínico-Universitario de Valladolid (Valladolid, Spain) Pedro L. Sánchez Hospital Universitario de Salamanca (Salamanca, Spain) Ricardo Sanz Ruiz Instituto de Investigación Sanitaria Gregorio Marañón Hospital General Universitario Gregorio Marañón (Madrid, Spain) Doris A. Taylor Texas Heart Institute (Houston, USA) Andreas W. Zeiher University of Frankfurt (Frankfurt, Germany) TECHNICAL DIRECTION Ana Fernández Baza Hospital General Universitario Gregorio Marañón C/ Doctor Esquerdo, 46. 28007 Madrid T. 00 34 91 426 58 82 / F. 00 34 91 586 82 76 anafdezbaza@cardiovascularcelltherapy.com TECHNICAL SECRETARIAT Viajes Orbiazul C/ Alonso Pesquera 2. 47002 Valladolid T. 00 34 983 30 91 22 / F. 00 34 983 29 53 51 housing@cardiovasculacelltherapy.com ACCREDITATION 22